Objective To investigate the serum levels of vitamins and antioxidative indexes as well as theircorrelations with nonalcoholic fatty liver disease (NAFLD),and to analyze the influence of different detection methods on same index detection result.Methods Eighty NAFLD patients diagnosed by ultrasound were chosen for detecting the indexes,including the levels of serum RBP,vitamin A (VA),vitamin D (VD,ELISA and enzyme donor competition method),vitamin E (VE),vitamin B12 (VB12),and antioxidative indexes,including malondialdehyde (MDA),superoxide dismutase (SOD,pyrogallol substrate method and WST-1 method) and reduced glutathione (GSH).Results Compared with the normal reference values,the deficiency rate of serum RBP,VA,VD,VE and SOD in NAFLD patients were 11.6％,38.4％,7.9％,86.0％ and 27.9％ respectively.The serum GSH level in the NAFLD group was lower than that in the healthy control group (P＜0.01).Detecting serum VD and SOD by different detection method found that the difference among different detection methods had statistical significance (P＜0.01).Conclusion The levels of serum VA,VE,GSH and SOD in NAFLD patients are significantly reduced.These detection indicators have the important significance for clinical diagnosis of NAFLD.