Objective To evaluate the effects of continued grateful emotion after discharge on the long-term positive experience and care burden of spouses of patients with brain tumors.Methods Totally 114 patients with brain tumor were selected as the research objects from October 2015 to October 2016. Random number table was used and all patients were randomly divided into the control group (n=56) and the observation group (n=58). Patients in the control group received routine health education and guidance, and two emotional emotional interventions were given to patients during the hospital; while patients in the observation group received increased emotional interventions according to the care burden of spouses of patients. The positive experience and care burden of two groups of spouses were evaluated by Caregiver Positive Experience Scale (PAC) and Caregiver's Burden Scale (CBI) 2 months, 3 months, and 6 months after discharge.Results There was no significant difference in positive experience and care burden of spouses between two groups (P>0.05); 3 months and 6 months after discharge, the scores of positive experience and care burden of spouses in the observation group were [(36.76±4.13), (36.71±4.21)]and [(27.07±4.62), (27.62±4.47)], and they were significantly better than those of the control group [(54.5±3.25),(25.36±3.17)and (54.75±6.27), (54.01±6.21)], the differences were statistically significant (t=7.847-8.587,P<0.05).Conclusions Emotional intervention during hospitalization can effectively improve the positive experience and care burden of spouses of patients. After discharge, emotional intervention can provide outpatient nursing care for the spouses of patients. There is a better long-term effect in improving the positive experience and care burden of spouses of brain tumor patients.