Objective To investigate the functions of microRNA-1 43 (miR-1 43)in esophageal cancer cell line ECA1 09.Methods ECA1 09 cells were transfected with negative control (NC),miR-1 43 mimics or miR-1 43 inhibitors.3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)assay was per-formed to evaluate the growth of ECA1 09 cells after transfection.Annexin V-FITC /PI apoptosis test kit was used to detect early apoptosis rate in ECA1 09 cells.Transwell migration and invasion assays were conducted to compare the migration and invasion capacity of ECA1 09 among different groups.Real-time PCR and Western blotting were used to analyze the mRNA and protein alteration after transfection.Results Three and four days after transfection,compared with NC (absorbance value:0.90 ±0.02 and 1 .09 ±0.07),miR-1 43 mimics inhibited ECA1 09 cell proliferation (absorbance value:0.66 ±0.05 and 0.80 ±0.04),while miR-1 43 inhibi-tors promoted cell proliferation (absorbance value:1 .1 3 ±0.09 and 1 .51 ±0.08),with statistical signifi-cances (F =49.1 6,P =0.000;F =1 00.34,P =0.000).Early-stage apoptosis rates of ECA1 09 transfected with NC,miR-1 43 mimics and miR-1 43 inhibitors were 3.42% ±0.72%,1 1 .63% ±1 .1 5% and 0.94% ± 0.1 0%,respectively,with statistical significance (F =1 51 .61 ,P =0.000).Meanwhile,compared with NC (migration cell number:336 ±1 3,invasion cell number:1 47 ±1 6),miR-1 43 mimics inhibited cell migration (1 48 ±1 6)and invasion (75 ±1 0),while miR-1 43 inhibitors promoted cell migration (51 0 ±1 4)and inva-sion (238 ±1 6),with statistical significances (F =470.99,P =0.000;F =90.04,P =0.000).Compared with NC (1 .00 ±0.00),miR-1 43 mimics down-regulated mRNA (relative expression level 0.22 ±0.08)and protein expression (relative expression level 0.46 ±0.08)of K-ras,whereas miR-1 43 inhibitors up-regulated mRNA (1 .55 ±0.1 2)and protein expression (1 .33 ±0.05)of K-ras (F =1 31 .36,P =0.000;F =88.1 7, P =0.000).Conclusion miR-1 43 functions as a tumor suppressor in esophageal cancer cell line ECA1 09, probably by down-regulating K-ras expression.

ABSTRACT:Objective To investigate application effect of quality care model in periopera-tive nursing in thyroid surgery patients.Methods A total of 50 cases with thyroid surgery in our hospital were divided into two groups with 25 people per group according to the willingness to re-ceive quality care.Control group implemented routine perioperative care while the experimental group applied quality care.Psychological situation,complications,relapse and satisfaction evalua-tion were evaluated.Results The SAS and SDS scores decreased significantly in the experimental group after comparison after treatment,there was significant difference (P <0.01);After treat-ment and care,SAS and SDS scores between the two groups was significant difference statistically significant (P <0.01);Wound infection,bleeding and breathing difficulties in the experimental group were significantly lower than that in the control group,there was statistically significant dif-ference (P <0.05);Patient care satisfaction rate in the experimental group was significantly better than that in the control group,there was a significant difference (P <0.01).Conclusion The ef-fect of quality care model in thyroid surgery in perioperative nursing is remarkable,and it is far-reaching significance.

ABSTRACT:Objective To investigate application effect of quality care model in periopera-tive nursing in thyroid surgery patients.Methods A total of 50 cases with thyroid surgery in our hospital were divided into two groups with 25 people per group according to the willingness to re-ceive quality care.Control group implemented routine perioperative care while the experimental group applied quality care.Psychological situation,complications,relapse and satisfaction evalua-tion were evaluated.Results The SAS and SDS scores decreased significantly in the experimental group after comparison after treatment,there was significant difference (P <0.01);After treat-ment and care,SAS and SDS scores between the two groups was significant difference statistically significant (P <0.01);Wound infection,bleeding and breathing difficulties in the experimental group were significantly lower than that in the control group,there was statistically significant dif-ference (P <0.05);Patient care satisfaction rate in the experimental group was significantly better than that in the control group,there was a significant difference (P <0.01).Conclusion The ef-fect of quality care model in thyroid surgery in perioperative nursing is remarkable,and it is far-reaching significance.