1.Advances in research of heat shock factor 1 and its relation with expression of heat shock protein.
Zhen-yu ZHANG ; Jun-jie CHEN ; Rong YU ; Ying CEN
Chinese Journal of Burns 2012;28(1):78-80
This article reviews the advances in the research of the structural characteristics and the activating process of heat shock factor 1 (HSF-1), the factors that influence the expression of HSF-1, and the relationship between HSF-1 and the expression levels of NF-κB and heat shock protein (HSP). The expression of HSF-1 could be regulated in several stages in the course of interconversion between its active and inactive status. Unusual expression of HSF-1 mediated by NF-κB can lead to the quantitative and functional change of HSP. The quantitative and functional variation of HSP caused by regulation of HSF-1 could influence the normal synthesis and the pathological changes induced by excessive synthesis of protective proteins in cells under stress. We expect that further research would help elucidate novel pathways about the genesis and evolution mechanism of keloid, and it may finally help to find a novel strategy in the treatment of keloid.
Animals
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DNA-Binding Proteins
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metabolism
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Heat Shock Transcription Factors
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Heat-Shock Proteins
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metabolism
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Humans
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Transcription Factors
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metabolism
2.Identification of heat stress transcription factors gene family in Setcreasea purpurea and analysis of its expression pattern under Cu2+ stress.
Guoying PENG ; Shan LU ; Kun YANG ; Wei WAN ; Changgan HUANG
Chinese Journal of Biotechnology 2022;38(1):238-251
Heat stress transcription factors (Hsf) family is one of the most important transcription factor families in plants, and plays an important role in the growth and development of plants when encountering abiotic stresses such as heat, drought, and heavy metals. In this study, 20 SpbHsf genes were identified from the full-length transcriptome database of Setcreasea purpurea, and the structure and function of the Hsf gene family were analyzed using bioinformatics tools and qRT-PCR. The results showed that all SpbHsf proteins were hydrophilic. There were 12 SpbHsf proteins located in the nucleus, and the content of α-helix and random coil in the secondary structure of all SpbHsf proteins was high. The SpbHsf genes are divided into three subfamilies, each of which contains unique conserved motifs. All SpbHsf proteins contain DBD and HR-A/B domains. Phylogenetic analysis showed that OsHsf in Oryza sativa protein had the highest homology with SpbHsf protein. All the 20 SpbHsf genes were expressed in the root tissues of S. purpurea. Among them, 8 were significantly up-regulated while 8 were significantly down-regulated under Cu2+ stress. This study may help better understand the function and expression pattern of the S. purpurea Hsf gene family.
Droughts
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Gene Expression Regulation, Plant
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Heat Shock Transcription Factors/metabolism*
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Humans
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Phylogeny
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Plant Proteins/metabolism*
3.Heat shock transcription factor family in plants: a review.
Nan ZHANG ; Yinghong WANG ; Zhimin WANG ; Zhenyu YUE ; Yi NIU
Chinese Journal of Biotechnology 2021;37(4):1155-1167
With the constant change of global climate, plants are often affected by multiple abiotic stresses such as heat stress, drought stress, cold stress and saline-alkali stress. Heat shock transcription factors (HSFs) are a class of transcription factors widely existing in plants to respond to a variety of abiotic stresses. In this article, we review and summarize the structure, signal regulation mechanism of HSFs and some research in plants like Arabidopsis thaliana, tomato, rice and soybean, to provide reference for further elucidating the role of HSFs in the stress regulation network.
Arabidopsis/metabolism*
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Droughts
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Gene Expression Regulation, Plant
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Heat Shock Transcription Factors/genetics*
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Plant Proteins/genetics*
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Stress, Physiological
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Transcription Factors/metabolism*
4.Effects of hsf1 genotype on the constitutive expression of alphaB-crystallin in mice myocardium.
Li LIU ; Hong-Hui ZHANG ; Guo-Xian DING ; Yun-Lin CHENG ; Liang-Jun YAN ; I J BENJAMIN
Chinese Journal of Applied Physiology 2004;20(1):30-33
AIMTry to clarify the effects of HSF1 gene on the constitutively expressed alphaBC.
METHODSTo investigate the levels of constitutively expressed alphaB-Crystallin (alphaBC) in hsf1 knockout (hsf1 -/-) and hsf1 wild type (hsf1 +/+) mice myocardium by Western blot and immunohistochemistry.
RESULTSThe alphaBC levels in hsf1 -/- and hsf1 +/+ were 68.42% +/- 4.16%, 100% +/- 7.58%, respectively (P < 0.05, cytosolic fraction), and 20.53% +/- 1.01%, 37.55% +/- 1.91%, respectively (P < 0.05, pellet fraction). The alphaBC signals decreased significantly in hsf1 -/- myocardium compared with hsf1 +/+ myocardium stained with fluorescence immunohistochemistry.
CONCLUSIONhsf1 is the important, but not the only factor, which mediates the constitutively expressed alphaBC.
Animals ; DNA-Binding Proteins ; genetics ; Female ; Genotype ; Heat Shock Transcription Factors ; Male ; Mice ; Mice, Knockout ; Myocardium ; metabolism ; Transcription Factors ; genetics ; alpha-Crystallin B Chain ; genetics ; metabolism
5.The relationship between the polymerization of HSF1 and the expression of IL-1beta, TNF-alpha mRNA of monocytes in fever rabbits.
Chinese Journal of Applied Physiology 2006;22(4):445-449
AIMFor further investigating of heat shock transcription factor 1 (HSF1) action in thermoregulation and its physiological mechanism.
METHODSThe relationship among the expression of HSF1 and interleukin 1beta (IL-1beta) mRNA and tumor necrosis factor alpha (TNF-alpha) mRNA in monocytes was studied during the different fever stages in rabbit fever model induced by LPS. The expressions of IL-1beta and TNF-alpha mRNA were measured by RT-PCR assay; HSF1 expression was measured by Western blot.
RESULTS(1) Intravenous injection of LPS produced a double-peak temperature arisen at 60 min and 180 min. (2) The expression of IL-1beta mRNA in monocytes had a peak at 160 min, while the peak of TNF-alpha mRNA expression occurred at 80 min after intravenous injection of LPS. (3) The content of the HSF1 trimer increased gradually after 160 min intravenous injection of LPS. The results indicated that the content of the HSF1 trimer was negative correlation with the expressions of IL-1beta mRNA and TNF-alpha mRNA, and the change in body temperature was a positive correlation with IL-1beta mRNA.
CONCLUSIONIt is possible that HSF1 limits the rise in body temperature by repressing the gene expressions of the endogenous pyrogen, IL-1beta and TNF-alpha.
Animals ; DNA-Binding Proteins ; metabolism ; Fever ; metabolism ; Heat Shock Transcription Factors ; Interleukin-1beta ; metabolism ; Male ; Monocytes ; metabolism ; RNA, Messenger ; genetics ; Rabbits ; Transcription Factors ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism
6.Influence of heat shock factor 1 gene transfection on the expression of inflammatory mediators in macrophages induced by burn serum.
Cheng-qun LUO ; Chao-qi YIN ; Jian-da ZHOU ; Quan-yong HE ; Jie ZHU ; Ping LI ; Tie-fu CHEN ; Hao PENG ; Yang-cheng XU ; Jia CHEN
Chinese Journal of Burns 2007;23(5):331-334
OBJECTIVETo investigate the influence of heat shock factor1 (HSF1) on gene expression of inflammatory mediators in RAW264.7 murine macrophage cells induced by burn serum.
METHODSSera were separated from blood of normal rats and rats with severe burns, and the recombinant vector pcDNA3. 1/HSF1 was constructed. RAW264.7 macrophages were divided into non-transfection group, vacant vector group (with burn and normal sera stimulation, respectively after vacant vector transfection) and recombinant vector group (with burn and normal sera stimulation, respectively after recombinant vector transfection). Some recombinant vector transfected macrophages without serum stimulation were prepared for the determination of HSF 1 expression with Western blotting. The mRNA expressions of TNF-alpha, HMGB 1 and IL-10 were determined with RT-PCR.
RESULTSThe cell line attained after recombinant vector transfection was comparatively stable,with partial activation of HSF 1. Burn sera markedly upregulated TNF-alpha, HMGB1 mRNA expression (0.910 +/- 0.100, 0.860 +/- 0.020, respectively), but downregulated IL-10 expression (0.430 +/- 0.010, respectively) in normal macrophages, while these genes maintained in a very low level in normal macrophages with normal serum stimulation . macrophages with recombinant vector transfection and burn serum stimulation could obviously inhibit the expression of TNF-alpha and HMGB 1, but enhance the IL-10 gene expression (0.130 +/- 0.100, 0.450 +/- 0.020 , 0.450 +/- 0.020, respectively )when compared with that with vacant vector transfection and burn serum stimulation (0.800 +/- 0.050, 0.880 +/- 0.030, 0.420 +/- 0.010, respectively).
CONCLUSIONHSF1 can inhibit the expression of some pro-inflammatory mediators in macrophages after a severe burns, indicating that appropriate upregulation of anti-inflammatory mediators might exert protective effects on the organism.
Animals ; Burns ; metabolism ; Cell Line ; DNA-Binding Proteins ; genetics ; Female ; Gene Expression ; HMGB1 Protein ; metabolism ; Heat Shock Transcription Factors ; Heat-Shock Response ; genetics ; Inflammation Mediators ; metabolism ; Interleukin-10 ; metabolism ; Macrophages ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Serum ; Transcription Factors ; genetics ; Transfection ; Tumor Necrosis Factor-alpha ; metabolism
7.Expression of heat-shock transcription factor 1 and X-linked inhibitor of apoptosis protein-associated factor-1 in gastrointestinal cancer.
Ting LI ; Cun-long CHEN ; Ji-de WANG ; Sheng-da CUI ; Dan-yu CUI ; Wen GUO
Journal of Southern Medical University 2008;28(3):487-490
OBJECTIVETo investigate the expressions of X-linked inhibitor of apoptosis protein (XIAP)-associated factor-1 (XAFI) and heat-shock transcription factor 1 (HSF1) and their relationship in human gastrointestinal cancers.
METHODSImmunoblotting was used to analyze the expressions of HSF1 and XAF1 in gastric and colon cancer tissues and in gastrointestinal cancer cells. The gastrointestinal cancer cells were tranfected with a eukaryotic expression vector containing HSF1 gene fragment or subjected to RNA interference to induce up- or down-regulation of HSF1 expression, and the consequence changes in XAF1 expression in the cells was measured. XAF1 expression was also assayed in the cells after stress stimulation for HSF1 expression.
RESULTSThe expression of HSF1 was higher in gastrointestinal cancer tissues than in normal tissues. The expression of XAF1 and HSF1 was inversely correlated in the cancer cell lines, and stress stimuli of the cells up-regulated the expression of HSF1 but down-regulated XAF1 expression.
CONCLUSIONHSF1 expression is increased in gastrointestinal cancer tissues to result in suppressed expression of XAF1, which may be one of the reasons for the low expression of XAF1 in association with the defect of the apoptosis mechanisms in the cancer cells
Cell Line, Tumor ; Colonic Neoplasms ; genetics ; metabolism ; pathology ; DNA-Binding Proteins ; biosynthesis ; genetics ; Heat Shock Transcription Factors ; Humans ; Immunoblotting ; Intracellular Signaling Peptides and Proteins ; Neoplasm Proteins ; biosynthesis ; genetics ; RNA Interference ; Stomach Neoplasms ; genetics ; metabolism ; pathology ; Transcription Factors ; biosynthesis ; genetics ; Transfection
8.Expression of HSF1 and XAF1 in gastro-intestinal cancer.
Ting LI ; Cun-long CHEN ; Ji-de WANG ; Sheng-da CUI ; Dan-yu CUI ; Wen GUO
Journal of Southern Medical University 2007;27(9):1447-1450
OBJECTIVEX-linked inhibitor of apoptosis protein (XIAP) To gastrointestinal (GI) investigate the expression of XAF1 and heat-shock transcription factors 1 èHSF1éand their relationship in human gastrointestinal cancers.
METHODSImmunoblotting was used to analyze the expression of HSF1 and XAF1 in either gastric or colon cancer tissue and GI cancer cell line. Transient expression of the HSF1-containing vector in GI cell lines and RNA interference were used to up/down-regulae the expression of the HSF1, and the subsequent expression of XAF1 was measured.
RESULTSThe expression of HSF1 was higher in GI cancers than in normal tissues. The expression of XAF1 and HSF1 was negatively correlated in GI cancer cell lines. Stress stimuli up-regulated the expression of HSF1 while the alteration of XAF1 expression was negatively correlated with HSF1 expression.
CONCLUSIONThe high expression of HSF1 in GI cancers is associated with suppressed expression of XAF1, which can be one of the mechanisms for low-expression of XAF1 and insufficient apoptosis in GI cancers.
Animals ; Base Sequence ; Cell Line, Tumor ; DNA-Binding Proteins ; genetics ; Gastrointestinal Neoplasms ; genetics ; metabolism ; pathology ; Gene Expression Regulation, Neoplastic ; Heat Shock Transcription Factors ; Humans ; Intracellular Signaling Peptides and Proteins ; Neoplasm Proteins ; genetics ; Oxidative Stress ; genetics ; Temperature ; Transcription Factors ; genetics
9.Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical cord-derived mesenchymal stem cells.
Jun LU ; Hui-Yue DONG ; Ling-Jing LIN ; Qing-Hua WANG ; Liang-Hu HUANG ; Jian-Ming TAN
Acta Physiologica Sinica 2013;65(6):600-606
The saturated free fatty acid (FFA), palmitate, could induce apoptosis in various cell types, but little is known about its effects on human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). Here, we investigated whether palmitate induced apoptosis and endoplasmic reticulum (ER) stress in hUC-MSCs. hUC-MSCs were stained by labeled antibodies and identified by flow cytometry. After administration with palmitate, apoptotic cell was assessed by flow cytometry using the Annexin V-FITC/7-AAD apoptosis detection kit. Relative spliced XBP1 levels were analyzed using semi-quantitative RT-PCR. The mRNA of BiP, GRP94, ATF4 and CHOP were analyzed by real-time PCR. Relative BiP and CHOP protein were analyzed using Western blot analysis. The results showed that hUC-MSCs were homogeneously positive for MSC markers; palmitate increased apoptosis of hUC-MSCs and activated XBP1 splicing, BiP, GRP94, ATF4 and CHOP transcription. These findings suggest that palmitate induces apoptosis and ER stress in hUC-MSCs.
Activating Transcription Factor 4
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metabolism
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Apoptosis
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DNA-Binding Proteins
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metabolism
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Endoplasmic Reticulum Stress
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Heat-Shock Proteins
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metabolism
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Humans
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Membrane Glycoproteins
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metabolism
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Mesenchymal Stromal Cells
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cytology
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drug effects
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Palmitates
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pharmacology
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Regulatory Factor X Transcription Factors
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Transcription Factor CHOP
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metabolism
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Transcription Factors
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metabolism
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Umbilical Cord
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cytology
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X-Box Binding Protein 1
10.Screen of inflammatory genes regulated by heat shock factor 1 and corroboration with SOCS3 gene.
Feng-xiu YU ; Hua-li ZHANG ; Guang-wen CHEN ; Qiu-peng WANG ; Yong-zhong SHI ; Ying LIU ; Qiu-juan LIANG ; Xian-zhong XIAO
Journal of Central South University(Medical Sciences) 2006;31(2):167-173
OBJECTIVE:
To screen the inflammatory mediators genes regulated by HSF1, and explore the mechanism of downstream genes regulated by HSF1.
METHODS:
HSF-/- and HSF1+/+ mice were injected with 15 mg/kg LPS intraperitoneally (ip), respectively, and were treated as previous after HSR. The total RNA of lung tissues were extracted and filtrated by SuperArray gene Microarry. The promoter of candidate genes were analyzed by transcription element search software to search for heat shock element (HSE). Select the suppressor of cytokine signaling 3 (SOCS3) with HSE. Macrophage cells were stimulated with 400 ng/mL LPS, and were treated as previous after HSR, then the total RNA was extracted respectively. RT-PCR and northern blot assay were performed to detect the expression levels of SOCS3 mRNA.
RESULTS:
Fifteen genes were repressed by HSF1, including 9 genes with complete HSE. Eleven genes were accelerated by HSF1 possibly, including 8 genes with complete HSE. The promoter of SOCS3 gene contained one complete HSE. LPS stimulation obviously increased the levels of SOCS3 mRNA in macrophages of RAW264.7 mice, which was inhibited by HSR and over-expression of HSF1.
CONCLUSION
HSR or HSF1 inhibits LPS induced expression of SOCS3 mRNA; HSF1 might inhibit LPS-induced expression of SOCS3 mRNA by binding to HSE in the promoter of SOCS3 gene.
Animals
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DNA-Binding Proteins
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genetics
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pharmacology
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Endotoxemia
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chemically induced
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genetics
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Heat Shock Transcription Factors
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Heat-Shock Proteins
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genetics
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Inflammation
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genetics
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Lipopolysaccharides
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Macrophages
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metabolism
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Mice
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Mice, Inbred BALB C
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Mice, Knockout
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Mutation
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RNA, Messenger
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biosynthesis
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genetics
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Signal Transduction
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Suppressor of Cytokine Signaling 3 Protein
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Suppressor of Cytokine Signaling Proteins
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biosynthesis
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genetics
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Transcription Factors
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genetics
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pharmacology