1.Up-expression of peroxisome proliferator-activated receptor-γand of retinoid X receptor-αenhances the inhibitory effect on leukemia cells
Yun GUO ; Ruicheng XU ; Tao HE
Journal of Leukemia & Lymphoma 2009;18(3):131-133
Objective To investigate the relationship between the expression of peroxisome proliferators-activated recepter-γ (PPARγ) and retinoid X receptor-or (RXRα) and the inhibitory effect of PLAB, ligand of PPARγ and 9-cisRA, ligand of RXRα on growth of human leukemia cell lines (HL-60, K562and U937) in vitro. Methods The antiproliferative effect was evaluated by MTT assay. The mRNA expression of PPARγ and RXRα was semi-quantified by RT-PCR. Results PPARγ and RXRα mRNA was both expressed in HL-60, K562 and U937 cells, and the expression in HI,-60 was significantly higher than that in K562 and U937. The significant inhibitory effect on the growth of HL-60 cells was observed in K562 and U937 cells. The combination group showed more inhibitory effect in HL-60 cells than PLAB alone(P<0.05).PLAB significantly up-regulates the expression of PPARγ in HL-60 cells, the expression of PPARγ and RXRα were higher in combination group than PLAB alone (P<0.05). Conclusion The expression of PPARγand RXRα in HL-60, K562 and U937 cell lines predicts their response to PLAB and 9-cisRA treatment, and the inhibitory effect is different in these three kinds of cell lines, which may be related to their ligandsmediated signal pathway.
2."Experience of Professor Yang Yufei Using ""Tri-Traffic Dredging Liver And Nourishing Kidney Formula""in the Treatment of Breast Cancer with Traditional Chinese Medicine Combined with Western Medicine"
Ning DING ; Yufei YANG ; Yun XU ; Bin HE ; Zhongning XU
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(4):734-737
Professor Y ang Y ufe i is the director and founder of the Oncology Department of X iyuan Hospital of China Academy of Chinese Medical Sciences. Professor Y ang Y ufei thinks highly of the treatment of breast cancer with harmonizing and dredging principle combining traditional Chinese medicine (TCM) and western medicine. She regards the basic pathogenesis of breast cancer is not only on traditional liver-kidney yin deficiency and liver-qi stagnation with spleen deficiency, but also three mechanisms which are breakdown of the normal physiological coordination be-tween the liver and kidney, heart and the kidney and Shaoyang. Professor Y ang gradually formed Tri-Traffic Dredg-ing Liver And Nourishing Kidney Formula base on tonification and purgation in combination. Y ang Y ufei thinks highly of combining treatment of TCM and western medicine, especially combined with endocrine therapy. Professor Y ang also pays much attention on immune function of patients and health education.
4.Acupuncture at ocular muscle points for 35 cases of trochlear nerve paralysis.
Ling-Yun ZHOU ; Ying HUANG ; He XU
Chinese Acupuncture & Moxibustion 2013;33(9):814-814
Acupuncture Points
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Acupuncture Therapy
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Adult
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Aged
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Eye
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physiopathology
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Female
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Humans
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Male
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Middle Aged
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Paralysis
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physiopathology
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therapy
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Trochlear Nerve Diseases
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physiopathology
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therapy
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Young Adult
5."Based on ""pulmonary gangrene"" warming yang and supporting, promoting blood circulation and Huoxuegongtan method for treatment of pulmonary alveolar proteinosis"
Xu JIAO ; Mo ZHANG ; Wanyun HE ; Wenjing LAI ; Yun LU
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2017;24(3):320-322
Pulmonary alveolar proteinosis (PAP) is a rare disease clinically, and at present no western medicine can effectively cure the disease. After the analysis of literatures related to Traditional Chinese Medicine (TCM) for treatment of the disease in the China National Knowledge Internet Database, it was discovered that the syndrome differentiation showed the disease closely associated with phlegm, blood stasis and yin deficiency. In this study, from view point of pulmonary gangrene TCM theory and treatment combined with PAP pathophysiological characteristics, it is emphasized that phlegm accumulation, blood stasis and yang deficiency are the key pathological manifestations of PAP, thus Yanghe decoction, Tounong powder, Kongxian pellet were used for treatment of one such case, the rational thinking in diagnosis and treatment of this patient being consistent with the idea learning western medicine for China use.
6.Effect of Dimethyiformamide(DMF) on Histological Structure and Enzymes Activity in Testis of Mice
Yun-He ZHENG ; Lu HUANG ; Xu-Jian HOU ; Al ET ;
Journal of Environment and Health 2007;0(09):-
Objective To explore the effect of dimethylformamide(DMF)on the histological structure and enzymes activity in the testis of mice.Methods The KM male mice were treated with DMF by gavage at the doses of 0,0.5,1 and 2 g/kg respectively, once a day,for 30 consecutive days.On day 31,the mice blood samples were collected through eyes and then the mice were killed. Two mice were randomly selected in each group and one testicle was sampled to do the pathological examination,the other one for enzyme activity determination,including succinic acid dehydrogenase(SDH),lactate dehydrogenase(LDH)and acid phosphatase (ACP).Results Compared with the control group,all treated groups showed a significant decrease in body weight(P
7.Influence of Liang-Xue-Huo-Xue (LXHX) Capsule on Apoptosis of Cultured Keratinocytes
Li-Yun SUN ; Bing-Xu DENG ; He WANG ; Kai CHEN
Chinese Journal of Dermatology 2003;0(10):-
Objective To investigate the pharmacological mechanism of LXHX capsule. Methods The skin specimens from psoriasis patients were examined with TUNEL (terminal deoxynucleotidyl trans-ferase-mediated dUTP-biotin nick end labeling) technique to detect the apoptosis of keratinocytes. PCNA (proliferating cell nuclear antigen) detecting kits was used, too. Then, the flow cytometry, with AnnexinV/PI and PI dyeing method, was used to analysis the effect of LXHX capsule on apoptosis in cultured keratinocytes. Results There was an increasing of both cell apoptosis and proliferation in psoriatic epidermis and LXHX capsule could induce apoptosis. Conclusions The keratinocyte apoptosis and proliferation both increased in psoriasis, which reach a new balance in a higher level. LXHX capsule could induce apoptosis in vitro, which may be one of the pharmacological mechanisms of LXHX in treating psoriasis.
8.Preparation of bio-derived bone and its histocompatibility
Yun NIU ; Xu HE ; Lihong ZHANG ; Ting GAO ; Hua XU ; Yulin LI
Chinese Journal of Tissue Engineering Research 2008;12(7):1385-1389
BACKGROUND: The ideal biomaterial means absence of cytotoxic effect and immunological rejection, degradation at right moment, and a well histocompatibility. Whether bio-derived bone can be used in vivo for long time and exerts functions deserves to be studied.OBJECTIVE: To investigate the local histocompatibility after bio-derived bone implanted into mouse and the effect on immunofunctions.DESIGN: A randomized controlled animal experiment. SETTING: Key Laboratory of Pathobiology, Ministry of Education, School of Basic Medical Sciences, Jilin University. MATERIALS: This study was performed at the Key Laboratory of Pathobiology, Ministry of Science, School of Basic Medical Sciences, Jilin University from March to July in 2006. Eighteen BALB/C mice (weighing 20±2 g, half male and half female), one male Kunming mouse (weighing 20 g), and one female rabbit (weighing 2.5 kg) were included in the experiment. All the experimental animals were provided by Laboratory Animal Center, School of Basic Medical Science, Jilin University. The disposal of the experimental animals in the test process accorded with ethical guidelines for the use and care of animals. Porcine cancellous bone (iliac bone) was purchased from the market. Iscove's Modified Dulbecco's Medium (IMDM, Hycolone, USA), fetal bovine serum (FBS, Gibco Co., Ltd, USA), methyl thiazolyl tetrazolium (MTT, Sigma, USA), and concanavalin A (ConA, Sigma Co., Ltd, USA) were used.METHODS: Bio-derived bone was prepared from commercial porcine bone. ① Eighteen BALB/C mice were randomly divided into three groups with 6 mice in each group: a control group (simple local muscle injury without implantation), a bio-derived bone implantation group ( implanting bio-derived bone into the lower limb), and a xenogenic bone implantation group (femoral bone from Kunming mouse was implanted into the muscle of lower limb). ② Twenty-one days after operation, the implant material and surrounding tissue were obtained for gross observation and haematoxylin-eosin staining to investigate the histocompatibility of bio-derived bone. Mouse immunofunction was assessed by complement-mediated cytotoxicity test. Absorbance was determined with an automatic ELISA reader at 570 nm to assess the cytotoxicity.MAIN OUTCOME MEASURES: ①Histocompatibility of implant surrounded tissue. ②Lymphocyte stimulation indices after induction of concanavalin A. ③ Cytotoxicity in each group after complement-dependent cytotoxicity test.RESULTS: Eighteen BALB/C mice were included in the final analysis. ①Histocompatibility of implant surrounded tissue: In the bio-derived bone implantation group, 21 days after bio-derived bone implantation, there were no presentation of congestion, degeneration, necrosis and diapyesis around the implant in gross, plenty of fibrous connective tissue invaded into the pores of the bio-derived bone, encapsulation and forming the fibrous capsule. A great quantity of neutrophils and macrophages were not detected around the implant by haematoxylin-eosin staining. Bio-derived bone was encapsulated with fibrous tissue, and part of the biomaterial began to degrade, and being replaced with fibrous tissue. Regarding xenogenic bone implantation group, necrotic tissue was detected in the cross-section of the muscle in gross. A lot of neutrophils, macrophages and necrotic tissue were detected around the implant by haematoxylin-eosin staining. ②Lymphocyte stimulation indices: The stimulation index of xenogenic bone implantation group was significantly larger than that of control group (P < 0.05). There was no statistical difference between the bio-derived bone group and the control group (P > 0.05). ③Cytotoxicity: The cytotoxicity of xenogenic bone implantaion group was significantly larger than that of control group (P < 0.05). There was no significant difference in cytotoxicity between the bio-derived bone implantation group and the control group (P > 0.05).CONCLUSION: The obtained bio-derived bone causes little immunoreactions, has no obvious cytotoxicity or inflammatory reactions, and possesses a good histocompatibility and bio-safety.
9.Bilateral traumatic hemorrhage of the basal ganglia.
Yun-Xu ZHANG ; Shu-Qin WEI ; Yan-Yun XING ; Qi LIU ; Wen-Jing HE
Chinese Journal of Traumatology 2016;19(4):247-248
Hemorrhage of the basal ganglia is common in hypertensive patients, and most of the cases are spon- taneous unilateral hemorrhage. Traumatic basal ganglia hemorrhage is uncommon, while bilateral hemorrhage of the basal ganglia after trauma is an extremely rare entity. This report described a rare case of bilateral hemorrhage of the basal ganglia after head trauma. We also analyzed the mechanisms and reviewed relative literatures.
Basal Ganglia Hemorrhage
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diagnostic imaging
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etiology
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Craniocerebral Trauma
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complications
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Female
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Humans
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Middle Aged
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Tomography, X-Ray Computed
10.Effects on activity of mouse NK cells by inhibitory Ly49 receptors blockade.
Journal of Zhejiang University. Medical sciences 2009;38(2):130-135
OBJECTIVETo observe the effects on antitumor activity of mouse NK cells and the mixed lymphocyte reaction with blocking the interaction between the inhibitory Ly49 receptor on mouse NK cell and MHC-I molecules on target cells with monoclonal antibody.
METHODSNK cells were isolated from splenocytes of C57BL/6J mouse with immunomagnetic method. YAC-1 and M1 cells were used as target cells, the cytotoxicity of NK cells was examined with mixed lymphocyte reaction before and after treatment of anti-Ly49 monoclonal antibody.
RESULTThe cytotoxicity of NK cells to M1 cell was significantly augmented (10.7% +/- 0.5% compared with 84.4 % +/- 2.9%), and the clone-forming capacity of M1 cell was inhibited remarkably (8.2 % +/- 2.2% compared with 94.0% +/- 3.3%) with the increase of concentration of the antibody (0 approximate, equals 60 microg/ml) (P <0.05). The difference of cytotoxicity to YAC-1 cells was only found between 0 microg/ml and 60 microg/ml groups (P <0.05). In mixed lymphocyte reaction, after pretreated with antibody, the proliferation of splenocytes of BALB/C mouse was inhibited (0.398 +/-0.025 compared with 0.128 +/- 0.014), and statistical difference was observed only between antibody groups of 0 microg/ml and 60 microg/ml (P<0.05).
CONCLUSIONBlocking the inhibitory Ly49 receptor on NK cells with monoclonal antibody augments the cytotoxicity to target cells.
Animals ; Antibodies, Monoclonal ; immunology ; pharmacology ; Cell Proliferation ; Cells, Cultured ; Female ; Genes, MHC Class I ; Killer Cells, Natural ; immunology ; Lymphocyte Culture Test, Mixed ; Lymphoma ; immunology ; pathology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; NK Cell Lectin-Like Receptor Subfamily A ; immunology ; Spleen ; cytology ; immunology