Objective To analyze the effects of alprostadil combined with benazepril in the treatment of diabetes and the effect on blood glucose fluctuation.MethodsEighty cases with diabetes were randomly divided into the control group and the observation group with 40 cases in each group.The control group was treated with benazepril while the observation group was additionally treated with alprostadil.The treatment effects, the changes of blood glucose, blood lipids and renal function were compared between the two groups, and the incidence of adverse reactions was recorded.ResultsThe total effective rate in the observation group was higher than that in the control group (P<0.05).After treatment, the fasting plasma glucose (FPG), postprandial 2h plasma glucose (2hPG) and glycosylated hemoglobin (HbA1c) were reduced to the normal level but the volatility differences of FPG and 2hPG in the observation group were lower than those in the control group (P<0.05).After 4 weeks of treatment, the total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) decreased while high-density lipoprotein cholesterol (HDL-C) increased (P<0.05) but the differences between the two groups showed no statistical significance.After treatment, the improvement of renal function indexes in the observation group was better than that in the control group (P<0.05), and the incidence of adverse reactions showed no significant difference between the two groups.ConclusionThe application of alprostadil combined with benazepril in the treatment of diabetes can reduce blood glucose fluctuation, improve renal function, and reduce the risk of diabetic nephropathy.

Proteome is a discipline which researchs the composition and the dynamic alteration of proteins.As the result of the progression in the clolorcetal cancer,the proteins expression showing a dynamic change process.The technique of proteome can perform qualitative and quantitative analysis for the proteins,and perform contrastive analysis for the normal hunman proteins metabolic.So we can screen the biomarkers that are associated with coloretal cancer progression.The article aims to summarize the proteome in the researching of the colorectal cancer.And summing up the biomarkers that are associated with the diagnosis,prognosis and treatment of the coloretal cancer.

Objective:To observe the effect of overdose fluoride on the expression of bFGF in rat's dental pulps.Methods:NaF at 20 mg/(kg?d) was given to each of 10 Wister rats by stomach perfusion for 8 weeks, another 10 rats were given the same volume of distilled water as the controls.After treatment,HE staining was used to observe the morphology of the teeth, immunohistochemical staining was adopted for study the expression of bFGF in the dental pulps of the rats.Results:The enamel layer and dentin layer of the incisors in NaF treated rats were thinner than those in the controls,the interglobular dentin was increased and the expression of bFGF in dental pulp and inner dentin was inhibited in the NaF treated rats(P

Objective To investigate the long-term protective effects of the waistband onto lumbar spins.Based on the practical situation,to search for some effective and convenient methods,such as wearing waistband and mutual massage,to prevent from lumbago for the army drivers worked in plateau.Methods 372 army drivers,young males,healthy and all worked in plateau,ware involved as the treatment group in the present study.Another 545 army drivers with the similar physical qualities,also worked in plateau,were selected as the control group.For the treatment group,a kind of special waistband was employed for the drivers,and mutual massage on hack of each other was performed among them;while in control group the army drivers were given no measures for lumbar protection and lumbago pre- vention.All the drivers in the both groups ware in normal driving conditions.The therapeutic effects of the waistband and the lumbago symptoms were investigated by epidemiologic survey 16 months later,Results The epidemiologic survey results showed that the incidence of lumbago in the drivers of control group(72.8%)was significantly higher than that in the treatment group(30.9%,P

This paper introduces a novel animal behavior monitoring system, in which a acoustooptic shielded room, computer multimedia technology, image acquisition technology, signal analysis technology are involved. This system can implement long-term non-interference automatic monitoring of the experimental animal with features of accurate recording, complete and objective analysis, and convenient and effective statistics. Applied widely to the animal model for post -traumatic stress disorder (PTSD), the system is designed to be used for other biomedical studies.

AIM: To detect surface electromyogram (EMG) signal accurately.METHODS: How to reduce noise in the detection of surface EMG signal was discussed. It has been explored to design an electrode unit by using methods of differential amplification, active electrode, filter and so on , andto investigate the electrode shape and the right placing method.RESULTS: The instrumentation amplifier AD620 was adopted to design a bipolar active electrode, which has been successfully used in the surface EMG detection.CONCLUSION: Experiments show that using active electrode can improve ratio of signal and noise, reduce noise and detect surface EMG signal effectively.

To explore the model of the standardized training for health management talents by referring to the standardized training for resident-doctors.This article analyzed the necessities of the model of the standardized training for health management talents from two aspects.After that,it elaborated on the significance of standardized training.At last,it proposed some ideas about constructing the standardized training for health management talents.

Objective To detect the expression of humar telomerase reverse transcriptase (hTERT) and telomerase activity in HeLa,MCF-7,SMMC7721 PC-3m and U2OS cell lines. Methods The techniques of immunochemistry and TRAP-ELISA were employed to detect the expression of hTERT and telomerase activity in different tumor cell lines.Results The positive rate of hTERT in HeLa cells(93.75%?3.10%)was significantly higher than that in U2OS cells(2.75%?0.96%),besides the other three cell lines showed an positive rates of hTERT in MCF-7(92.50%?2.65%),SMMC7721 (53.75%?2.22%)and PC-3m(23.50%?2.89%); Meanwhile,the telomerase activity of HeLa cells (94.58%?3.49%) was also much higher than that of U2OS(3.02%?0.43%),likewise the telomerase activities of the other three cell lines (MCF-7,SMMC7721,PC-3m) were 73.90%?4.50%,66.67%?3.35% and 50.62%?1.96%, respectively. Conclusion The expression of hTERT and telomerase activity show obvious differences among five tumor cell lines,suggesting that telomerase inhibitors cannot effect on all the tumor cell lines.

OBJECTIVE To study and analyze the risk factors of surgical incision infection. METHODS All of 17 044 aseptic surgery patients from 2004 to 2006 were investigated. RESULTS Totally 36(0.21%) cases occurred infection with class Ⅰ surgical site.The incision infection ratio was 0.24%,0.22% and 0.18% in 2004,2005,and 2006,respectively.The operation time more than three hours occurred in 17 patients,two and three hours were in 12 cases,and less than two hours in the seven cases,accounted for 47.23%,33.33% and 19.44%,respectively. CONCLUSIONS The risk factors of incision infection after aseptic were as age,operation times,seasons,perioperative administration and underlying diseases.In order suroery to prevent the incition infection in aseptic surgery,we should pay more attention to management and education among medical staff.

AIM:To investigate the effects of brain-derived neurotrophic factor(BDNF)on extracellular proteolytic enzymes including matrix metalloproteinases(MMPs)and serine proteases,in particular,the urokinase-type plasminogen activator(uPA)-plasmin system in a human umbilical vein endothelial cell(HUVEC)model.METHODS:The HUVEC was activated with different doses of BDNF(25-200 ?g/L)for different time period(6-48 h).Reverse transcriptase-polymerase chain reaction(RT-PCR)was used to assay MMP-2,MMP-9,TIMP-1,and TIMP-2 mRNA in HUVEC.The cultured conditioned medium was analyzed for MMP and uPA activity by gelatin zymography and fibrin zymography,respectively.uPA,PAI-1,TIMP-1,and TIMP-2 were quantified by Western blotting analysis.RESULTS:The stimulation of serum-starved HUVECs with BDNF caused marked increase in MMP-2 and MMP-9 mRNA expression and induced the pro-MMP-2 and pro-MMP-9 activation without significant differences in proliferation.However,BDNF had no effect on TIMP-1 and TIMP-2 production.BDNF increased uPA and PAI-1 production in a dose dependent manner up to 100 ?g/L,while effects of 200 ?g/L were approximately equal to those of 100 ?g/L.BDNF stimulated uPA and PAI-1 production beyond that in control cultures from 12 h until 48 h after BDNF addition.Protease activity for uPA was also increased by BDNF in a dose dependent manner.CONCLUSION:BDNF activates MMP and uPA/PAI-1 proteolytic network in HUVEC.