1.Application and development of non contact angle - wide viewing system in vitreous retinal surgery
Rong-Hua, HE ; Guo-Hong, ZHOU ; Li, KONG ; Jing, LI
International Eye Science 2016;16(7):1295-1297
Wide- angle viewing system as an important auxiliary device can clearly observe the whole fundus field of vision in vitreous surgery, which enable vitreoretinal surgery more efficient, safer and more effective. So it has very high application value in ophthalmologic operation. In this paper, we studied the development and application of wide - angle viewing system in vitreoretinal surgery in recent years, from which we summed up the advantage of non - contact wide - angle viewing system in clinical field, and pointed out the shortcomings. The ultimate goal is to make the non -contact wide - angle viewing system better applied in vitreous surgery.
3.The effect of HMGB1 on mitochondrial biogenesis in anoxia environment
Rong XU ; Xingbo HE ; Zongrong HUA ; Yao LIU
Journal of Medical Postgraduates 2017;30(3):245-250
Objective High mobility group box-1 protein ( HMGB1) plays an essential role in regulating energy metabolism of tumor cells via affecting mitochondrial autophagy .The aim of this study was to explore the effect of HMGB 1 on mitochondrial biogene-sis and cell energy metabolism in anoxia environment . Methods HepG2 cells were divided into normoxia control group ( cells were cultured in a culture box containing 5%CO2) , hypoxia control group ( cells were cultured in a culture box containing 1%O2+5%CO2+94%N2 ) , hypoxia HMGB1 siRNA group ( cells were cultured in a culture box containing 1% O2+5% CO2+94% N2 after transfected with HMGB1 siRNA) and hypoxia NC siRNA group ( cells were cultured in a culture box containing 1%O2+5%CO2+94%N2 after trans-fected with negative control siRNA ) .MTS assay was carried out to measure cell proliferation rate .The alterations of mitochondrial bio-genesis associated proteins were detected by RT-PCR and western blot.Mitochondrial density and morphology were determined by transmission electron microscopy (TEM).The ATP content in whole cell extracts was determined with a colorimetric ATP detection kit . Results Compared with the hypoxia control and hypoxia NC siR-NA group, the proliferationof hypoxia HMGB1 siRNA group was significantly inhibited, especially in 48 h and 72 h(P<0.05).Com-pared with hypoxia control group and hypoxia NC siRNA group , the expression of PGC 1α, NRF1and TFAM in hypoxia HMGB1 siRNA group were decreased significantly ( P<0.05) .Western blot results showed that , compared with normoxia control group , the expressions of PGC1α(0.494±0.210 vs 0.090±0.020), NRF1(1.080±0.470 vs 0.581±0.190)and TFAM(1.585±0.340 vs 0.792±0.350) protein in hypoxia 24 h group were increased obviously ( P<0.05) .Compared with hypoxia control group and hypoxia NC siRNA group , the expres-sions of PGC 1α, NRF1 and TFAM protein in hypoxia HMGB1 siRNA group were significantly decreased (P<0.05).Compared with hypoxia control group , the content of ATP in the HMGB 1 siRNA hypoxia group was significantly decreased , and hypoxia 12 h and 24 h were the most obvious ( P<0.05) . Conclusion HMGB1 could maintain cell energy metabolism by regulating mitochondrial biogene-sis so that cells could continue to proliferate in adverse anoxia environment .
4.Imageology and significances of rotational axes of distal femur on Chinese adults with total knee arthroplasty.
Bing-hua SONG ; Jun-ying SUN ; Zeng-liang NI ; Bin HE ; Jian-qun HE ; Rong SHAO
China Journal of Orthopaedics and Traumatology 2016;29(1):41-47
OBJECTIVETo explore clinical significance of rotational axis of distal femur on Chinese adults in total knee arthroplasty (TKA).
METHODSThere were 86 Chinese adults (106 normal knees) including 47 males (53 knees) and 39 females (53 knees), 54 knees were on left and 52 on right. The CT scan was employed in the distal femur. The scan direction was aligned to be on the plane perpendicular to the mechanical axis of the femoral. The CT images of cross sections across lateral and medial femoral epicondyle were moved to personal computer,lateral angle between anterior posterior line (APL) and surgical transepicondylar axis (STEA) (ATA),lateral angle between posterior condylar line (PCL) and APL (APA), angle between perpendicularity of APL and PCL (A-PA), posterior condylar angle (PCA), condylar twist angle (CTA), angle between clinical transepicondylar axis (CTEA) and STEA (CSA) were measured. These values were divided into different groups according to gender and side, the values of CTA, PCA, A-PA, angle PT (varus of tibia plateau), constant 3, ATA, APA and constant 90° were compared by statistically. A-PA and PCA, and CTA were analysed statistically with the liner regression, the relationship among CTEA, STEA ,PCL, APL and PLP were performed to assess by liner regression.
RESULTSATA was (89.79 ± 1.22)°, APA was (84.84 ± 1.83)°, A-PA was (5.16 ± 1.83)°, PCA was (4.80 ± 1.23)°, CTA was (8.23 ± 1.40), CSA was (3.45 ± 0.68)°. All the parameters had no differences on sex and side,but CSA had difference on male and female. There was no difference among angle PT, PCA, A-PA. There was significant difference in CAT, constant 30 and angle PT, PCA,A-PA. There was no difference between ATA and constant 90°, but there was difference between APA and constant 90°. There was relativity between PCA and CTA, and also PCA and A-PA, CTA and A-PA. There was significant relativity between STEA and CTEA, between STEA and APL, between STEA and PCL, and also between APLP, APL and PCL, but there was no significant relativity between PCL and CTEA.
CONCLUSIONTKA for Chinese, the section of femoral posterior condyle should be external 5° to obtain the optimum rotational orientation. The property is different entirely between STEA and CTEA, the rotational alignment is not performed according to parallel to the CTEA in distal femur. Among STEA, APL, PCL, the STEA is the most reliable mark ofrotational alignment of the distal femur, and the PCL is the less reliable mark.
Adult ; Arthroplasty, Replacement, Knee ; Female ; Femur ; anatomy & histology ; diagnostic imaging ; Humans ; Male ; Middle Aged ; Rotation ; Tomography, X-Ray Computed
6.Analysis of varieties and standards of labiatae medicinal plants used in Tibetan medicine.
Jun-wei HE ; Lan CAO ; Hua-rong ZHOU ; Wei-hong ZHONG ; Guo-yue ZHONG
China Journal of Chinese Materia Medica 2015;40(7):1419-1424
In this paper, an analysis was made on the varieties and standards of labiatae medicinal plants used in Tibetan medicine. The results showed 71 species of labiatae plants in 21 genera (including varieties) recorded in relevant literatures, involving 44 varieties of medicinal materials. Specifically, seven species (9.9%) were intersected with traditional Chinese medicines (TCM), 19 varieties (43%) were recorded in Chinese medicinal material standards at all levels, and 27 species (38%) were source plants. In Tibetan medicine standards and literatures, there are great differences between Tibetan names and translated Chinese names and among varieties of source plants. Apart from a few of varieties intersected with traditional Chinese medicines had complete standards and regulations in Chinese Pharmacopoeia, most of species only had characters, microscopic, physical and chemical identifications in Standards Issued by Ministry of Health-Tibetan Medicine, Tibetan Medicine Standard and local standards. Therefore, the Tibetan medicinal material variety-source specification and quality standard system shall be promoted on the basis of literatures research, investigations for resources and current applications and modern pharmaceutical studies.
Drugs, Chinese Herbal
;
chemistry
;
pharmacology
;
Humans
;
Lamiaceae
;
chemistry
;
classification
;
Medicine, Tibetan Traditional
;
standards
;
Phytotherapy
;
standards
;
Plants, Medicinal
;
chemistry
;
classification
7.The synergistic effect of lidamycin and rituximab on human B cell lymphoma.
Yi-Ran SUN ; Sheng-Hua ZHANG ; Rong-Guang SHAO ; Hong-Wei HE
Acta Pharmaceutica Sinica 2014;49(2):198-203
This study aimed to investigate the synergistic effect of lidamycin (LDM) and rituximab on human B cell lymphoma Ramos cells. Cell proliferation was measured using MTS assay, cell apoptosis was analyzed by Annexin V-FITC/PI assay, the expression of apoptosis related proteins was analyzed by Western blotting, and the in vivo lymphoma inhibition was verified using BALB/c mice inoculated via tail vein using Ramos cells which stably expressed pEGFP-N1 plasmid. The results showed that, after the pretreatment with rituximab for 48 h, rituximab and LDM showed significantly synergistic effects on cell proliferation. Cells in combined treatment group had a higher apoptosis rate than that in LDM treatment group. Compared with the LDM treatment group, the expression of apoptosis-related proteins such as Cleaved caspase-3, Cleaved caspase-7, Cleaved caspase-9 and Cleaved PARP in combined treatment groups increased, and expression of cIAP-2 and Bcl-2 decreased. The result of in vivo experiment showed that, in the combined treatment group, the survival time of BALB/c mice was significantly longer than the mice in control group and LDM treatment group, and the degree of tumor accumulation and metastasis to lymph nodes and spleen was lower.
Aminoglycosides
;
pharmacology
;
Animals
;
Antibiotics, Antineoplastic
;
pharmacology
;
Antineoplastic Agents
;
pharmacology
;
Apoptosis
;
drug effects
;
Caspase 3
;
metabolism
;
Caspase 7
;
metabolism
;
Caspase 9
;
metabolism
;
Cell Line, Tumor
;
Cell Proliferation
;
drug effects
;
Drug Synergism
;
Enediynes
;
pharmacology
;
Humans
;
Inhibitor of Apoptosis Proteins
;
metabolism
;
Lymphoma, B-Cell
;
metabolism
;
pathology
;
Mice
;
Mice, Inbred BALB C
;
Mice, Nude
;
Neoplasm Metastasis
;
Neoplasm Transplantation
;
Poly(ADP-ribose) Polymerases
;
metabolism
;
Proto-Oncogene Proteins c-bcl-2
;
metabolism
;
Random Allocation
;
Rituximab
;
pharmacology
8.Significance and distribution of lymphopenia and anti-lymphocyte antibody in systemic lupus erythematosus
Chun LI ; Xiaoyan LU ; Jing LI ; Jing HE ; Hua YE ; Rong MU ; Zhanguo LI
Chinese Journal of Rheumatology 2009;13(5):316-319,前插2
Objective To investigate the significance and distribution of lymphopenia and antilymphocyte antibody(ALA) in systemic lupus erythematosus (SLE) and to explore the role of ALA in lympho penia.Methods One hundred and ten in-patients who were admitted during February 2003 to February 2008 were retrospectively reviewed.Indirect immunofluorescence test was used to detect ALA.Results ① Lymphopenia (<1.5×109/L) was observed in 68.2% patients.Lymphopenia was associated with skin rashes,serositis,renal involvement,NPSLE,leucopenia,ANA,ds-DNA antibody,ESR and IgG levels.The lymphocyte count and SLEDAI scores was more closely correlated than total white blood cell counts.②ALA was positive in 51/110 (46.4%) patients.ALA was associated with renal involvement,NPSLE,WBC count,C3 level decrease,ANA,dsDNA antibody and SLEDAI scores.Conclusion Lymphopenia is significantly associated with SLEDAI scores.ALA is possibly one cause of lymphcytopenia.In addition,ALA is also a parameter for disease activity,and is associated with organ involvement and outcomes.
9.Treating Persistent Henoch-Schonlein Purpura Nephritis by Injecting Methylprednisolone into Intra-Renal Capsule in Children
zhi-hui, LI ; jin-hua, HE ; yan, YIN ; cui-rong, DUAN
Journal of Applied Clinical Pediatrics 2006;0(17):-
Objective To evaluate the results of treating the persistent Henoch-Schonlein purpura nephritis(HSPN) by injecting methylprednisolone into intra-renal capsule in children.Methods Twenty-two patients(aged from 6 to 13 years) with persistent HSPN were randomly divided into 3 groups.Group I was treated with prednisone;group Ⅱ was treated with high dosage methylprednisolone by venous injection,while group Ⅲ was treated by injecting methylprednisolone into intra-renal capsule.The 24 h urinary protein excretion,the levels of serum albumin and creatinine,or the blood cholesterole in children with persistent HSPN were detected at the beginning,4 weeks and 8 weeks of the study.The blood pressure,body weight were detected in the study duration.Results The values of 24 h urinary protein excretion were(2.35?1.09),(0.97?0.37),and(0.99?0.52) g(P
10.Angiotensin Ⅱ Stimulate the Expression of Integrin-Linked Kinase in Rat Glomerular Mesangial Cell
zhi-hui, LI ; tian-hui, WU ; cui-rong, DUAN ; jin-hua, HE ; yan, YIN
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To investigate the effect of angiotensin Ⅱ(Ang Ⅱ)on the expression of integrin-linked kinase(ILK)in rat glomerular mesangial cells.Methods SD rat glomerular mesangial cells were separated and cultured.They were treated with 10-7 mol/L Ang Ⅱ in various time-point(0,12,24,48,72 h),and then cultured rat glomerular mesangial cells were treated 48 h with Ang Ⅱ in various concentration(0,10-11,10-9,10-7,10-5 mol/L).Then total protein expression in rat glomerular mesangial cells stimulated by Ang Ⅱ at every time-point and every concentration-point was extracted.The expression of ILK protein was measured by Western blot.Results 1.Expression of ILK protein in rat glomerular cells stimulated by Ang Ⅱ in various time-point,or various concentration was significantly increased.In group of 10-7 mol/L Ang Ⅱstimulated cells,the protein semiquantity of ILK at 0,12,24,48,72 h respectively was 0.18?0.02,0.37?0.07,0.90?0.10,1.73?0.12,and 1.72?0.13(F=166.78 P