Objective To investigate the treatment effect of daily low dose of sildenafil treating interstitial cystitis .Methods 68 patients with interstitial cystitis in our hospital from June , 2012 to December, 2013 were divided into observation group and con-trol group by digital randomized method, 34 cases in each group.Observation group adopted daily dose of sildenafil 0.25 g treatment every day, and control group adopted the same dose of starch placebo every day.Two groups were treated for 3 months.Curative effect, micturition number a day before and after the treatment, the maximum bladder capacity, interstitial bladder inflammation shape index and problem index score ( ICSI, ICPI) , pelvic pain and urinary frequency score ( PUF) and quality of life scores ( QOL) of two groups were compared.Results The rate of significant curative effect and total effective rate of observation group were significantly higher than that of the control group, and the difference was statistically significant (p<0.01).Micturition number every day, the maximum bladder capacity, ICSI, ICPI, PUF and QOL scores of the two groups before treatment had no statistical significant differ-ences(p>0.05).Maximum bladder capacity and QOL scores of observation group after treatment were (265.44 ±43.85) ml and (44.15 ±4.69) respectively, which were higher than the (92.45 ±25.71) ml and (22.14 ±2.97) of the control group after treat-ment and (94.12 ±23.95)ml and (22.38 ±3.18) of observation group before treatment;Urine per day, ICSI, ICPI and PUF of ob-servation group after treatment is lower than that of the observation groups after treatment and that of the control group before treat-ment, and the difference had statistically significant (p<0.05).Conclusion Daily low dose of sildenafil for treating women with in-terstitial cystitis is worthy of clinical use for it can effectively relieve clinical symptoms and improve patient's life quality.

Objective To study the clinical features and diagnostic analysis of 30 cases with refractory myco-plasma pneumonia.Methods 60 patients with mycoplasma pneumonia treated in our hospital during 2012.03 to 2014.03 were selected, and 30 cases were diagnosed as normal mycoplasma pneumonia and the others as refractory mycoplasma pneumonia.The clinical manifestations, percentage of neutrophils, peripheral blood leukocytes , C -re-active protein (CRP) level, and chest radiographic were retrospectively analyzed , and the clinical judging indicators of refractory mycoplasma pneumonia were obtained by multivariate logistic regression analysis .Results Considering the time of fever, the difference between two groups was statistically significant (χ2 =12.27, p <0.01).The differ-ence of percentage of neutrophils , peripheral blood leukocytes and CRP between the two groups were all significant (p <0.05); Logistic regression analysis showed that time of fever >10 d, CRP >40 mg /L, appearance of large high -density lung opacities were the judging indicators of mycoplasma pneumonia refractory .Conclusion Time of fever, CRP level and imaging Findings are the judging indicators of mycoplasma pneumonia refractory .

The changes of the spinal cord blood flow(SCBF),the content of TXB2 and 6-keto-PGF1a of the injured segment of the cord and other neurological manifestations were observed on a rat model of spinal cord injury(SCI)established with Allen's weight drop(50g-cm)method and the effects of indomethacin on these changes were studied.It was found that SCBF was significantly reduced in the first 2 hours after SCI and further reduced in the 4th ~ 8th hour.Increase of TXB2 was observed in the 1st hour and reached the peak in the 4th hour.The level of 6-keto-PGF1a was also increased in the 1st hour and maintained at that level for 24 hours.The changes of TXB2/6-keto-PGF1a was similar to those of TXB2.There was a negative correlation of SCHF with TXB2 content and TXB2/6-keto-PGF1a ratio.The intravenous injection of 10mg/kg indomethacin could inhibit the increase of TXB2 content and increase 6-keto-PGF1a content relatively.It could also alleviate or retard the decrease of SCBF after SCI and improve the motar function of the hind limbs of the rats.These findings suggest that indomethacin can improve SCBF and promote the recovery of neurological functions through its regulatory effects on the levels of TXB2 and 6-keto-PGF1a

This study was aimed to establish an identification method between leaflets of Dimocarpus longan and leaflets of Litchi chinensis. The leaflet morphological-venation pattern (LMVP) and quantitative analysis were reliable identification method for Chinese herbs. The results showed that the main differences of leaflets of Dimocarpus lon-gan were the eucamptodromous camptodromous pinnate venation; the secondary veins gradually changed into little near margin and a few brochidodromous; the type of tertiary vein was percurrent or reticulate. The main differences of leaflets of Litchi chinensis were brochidodromous camptodromous pinnate venation; the course of the secondary veins was sinuous or zigzag, and abruptly curved then linked; the type of tertiary vein was reticulate. With three groups of key differences mentioned above, both plants can be successfully identified from each other. The accuracy of identification results (AC) was from 98.1% to 100%. The agreement rate for observation (ARO) was from 98.5% to 100%. And the Kappa value was from 0.97 to 1.00. It was concluded that the established LMVP is simple, rapid, e-conomic and reliable in the identification between leaflets of Dimoc arp us longan and leaflets of its confused herb Litchi chinensis.

To explore the characteristics of hematological abnormalities in patients with systemic lupus erythematosus (SLE) through retrospective analysis.A total of 445 SLE patients were recruited and divided into 5 groups of low white blood cell,immune thrombopenic purpura,anemia,pancytopenia and control without hematological abnormalities.Among them,351 (78.9％) had hematological abnormalities and 94 (21.1％) without hematological abnormities.The prevalence of anemia,leucocytopenia,thrombocytopenia and pancytopenia were 56.0％ (246/445),27.6％ (123/445),13.5％ (60/445) and 8.5％ (38/445) respectively.The incidence of renal damage in anemia group was significantly higher than that in control group (P ＜ 0.01).SLE patients with hematological abnormalities had lower complement C3 levels (P ＜ 0.05) and higher systemic lupus erythematosus disease activity index (SLEDAI) (P ＜ 0.01).Anemia is the most common findings in SLE patients with hematological abnormalities who have lower complement C3 levels and higher SLEDAI.

Antigens, CD34 ; metabolism ; Choriocarcinoma ; pathology ; Diagnosis, Differential ; Female ; Hemangioma ; metabolism ; pathology ; Humans ; Keratin-18 ; metabolism ; Mesenchymoma ; pathology ; Placenta ; pathology ; Placenta Diseases ; metabolism ; pathology ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism ; Pregnancy ; Pregnancy Complications, Neoplastic ; metabolism ; pathology ; Young Adult

Objective To investigate the effect of left lateral segmentectomy combined with fiber choledochoscope for hepatobiliary calculus.Methods Eighty-seven patients with hepatobiliary calculus who underwent hepatectomy from December,1998 to December,2008 were reviewed retrospectively.Forty-six patients underwent the section of common bile duct to remove calculus combined with left lateral segmentectomy and fiber choledochoscope( Group A),41 patients underwent left hepatectomy and section of common bile duct to remove calculus( Group B).The postoperative stone residual rate,complications and long-term outcome were compared between the two groups.Results The postoperative stone residual rate of either group was zero.Intraoperative bleeding ( 401.1 ± 180.4ml) ,operative times ( 202.5 ±36.6 rmin) of group A were significantly lower than those of the group B (515.9 ± 200.6ml and 257.3 ± 42.9min) ( P ＜ 0.001 and 0.01,respectively) .Hospital stays of group A was lower than that of group B,but this is no statistical significance ( P ＞ 0.05 ) .The incidence of postoperative complications were similar in the two groups( 15.2% v.s.24.4% ,P ＞ 0.05 ).The effective rates of the two groups were similar (91.3 % VS 92.7 %,P ＞ 0.05 ).Conclusions The long-term and short-term outcome of left lateral segmentectomy combined with fiber choledochoscope for hepatobiliary calculus is similar to that of left hepatectomy ,and can successfully shorten the hospital stay and reduce the hospital fee.

OBJECTIVE:To improve the management of automatic medicine packaging machine(ATC),so as to improve drug dispensing management. METHODS:The short-plate effect of“cask theory”was used to analyze the deficiency of ATC in soft-ware,hardware and management;pertinent improvement was conducted and its effects were evaluated. RESULTS & CONCLU-SIONS:Considering the deficiency of ATC software function,automatic form export function had been set up additionally,involv-ing form of adding and dismounting drug information,form of repeated medical order. Some improvement were carried out for fre-quent machine halt in hardware,such as adjusting the height of drug box and using spare drug groove;some carried out for incon-sistency between the accounts and drugs due to management defects,such as fixing post,improving adding drug information input. Compared with before improvement,daily times of machine halt during packaging had cut from 13.8 times to 2.3 times(decreased by 83.3%);daily total time of machine halt had shortened from 97.4 minutes to 9.6 minutes(shortened by 90.14%);the rate of packaging had increased from 14.3 packages/min to 23.33 packages/min(increased by 62.9%);the management of drug stock be-came more standard. The application of“cask theory”in the improvement of ATC can promote the quality of drug dispensing man-agement.

New antibiotics with novel modes of action and structures are urgently needed to combat the emergence of multidrug-resistant bacteria. Bacterial signal peptidase I (SPase I) is an indispensable enzyme responsible for cleaving the signal peptide of preprotein to release the matured proteins. Increasing evidence suggests that SPase I plays a crucial role in bacterial pathogenesis by regulating the excretion of a variety of virulent factors, maturation of quorum sensing factor and the intrinsic resistance against beta-lactams. Recently, breakthrough has been achieved in the understanding of three-dimensional structure of SPase I as well as the mechanism of enzyme-inhibitors interaction. Three families of inhibitors are identified, i.e. signal peptide derivatives, beta-lactams and arylomycins. In this article, we summarize the recent advance in the study of structure, activity and structure-activity relationship of SPase I inhibitors.

Purpose To explore the value of three vessels and trachea view in rapid screening of aortic arch malformations. Materials and Methods 51 fetal subjects who were prenatally diagnosed with aortic arch abnormalities were enrolled, and their diagnoses were confirmed by postnatal echocardiography or autopsy results after induction. Targeted fetal cardiac examinations were conducted under obstetric ultrasound examination conditions, ultrasound images were restored and compared with sonographic features of normal aorta. Results Among the 51 fetus cases with aortic arch anomalies, right-oriented aortic arch with left subclavian artery vagus was shown in 17 cases, left-oriented aortic arch with right subclavian artery vagus in 2 cases, double aortic arch in 2 cases, aortic arch disarticulation in 6 cases, and aortic arch coarctation in 24 cases. Diagnostic accuracy of ultrasound was 94.1%. Sonographic changes were displayed in three-vessel or three vessels and trachea view in all cases. Conclusion Three vessels and trachea view plays an important prompt role in ultrasound diagnosis for fetal aortic arch abnormalities. But when it is manifested as the“1oo”sign, it will be rather difficult for the differential diagnosis of aortic arch disarticulation and severe aortic arch coarctation.