OBJECTIVE: To explore the material foundation of kidney-yang-deficiency syndrome. METHODS: Two kinds of rat models of deficiency of kidney-yang were induced by adenine intragastric administration (model I) and hydrocortisone intramuscular injection (model II). One hundred rats were randomly divided into normal control group, model I group, low-dose model II group, medium-dose model II group and high-dose model II group. After model establishment, contents of serum creatinine (SCr), blood urea nitrogen (BUN), and urine creatinine (UCr) were detected by automatic biochemistry analyzer; freezing point method was used for 24-hour urinary osmotic pressure (Uosm) testing and kaolinite method was used to detect the content of urinary 17-hydroxycorticosteroid (17-OHCS). Expression of aquaporin-1 in renal tissue was observed by using real time reverse transcription polymerase chain reaction. RESULTS: The rats of model groups had the characteristics of kidney-yang deficiency syndrome. The contents of SCr and BUN were significantly higher in model I group than in normal control group and three model II groups (P<0.05), and UCr and Uosm were significantly lower (P<0.05). The level of urinary 17-OHCS and expression of aquaporin-1 in renal tissue were decreased in the model I group (P<0.05) as compared with the normal control group. Compared with the normal control group, the content of SCr was increased and urinary 17-OHCS was decreased in the medium-dose model II group, but the expression of aquaporin-1 was increased in three model II groups with a dose-dependent manner. CONCLUSION: Aquaporin-1 may be one of the material foundations of kidney-yang-deficiency syndrome.

At present, Chinese medical field faces with an important problem of how to correctly handle the relationship between medical and scientific research. Academician Li Lianda advocates doctors doing scientific research under the premise of putting the medical work first. He points out that there are many problems in the process of doctors doing scientific research at present such as paying more attention to scientific research than medical care, excessively promoting building scientific research hospital, only paying attention to training scientific talents, research direction be flashy without substance, the medical evaluation system should be improved and so on. Medical, scientific research and teaching are inseparable because improving medical standards depends on scientific research and personnel training. But not all doctors need to take into account of medical treatment, scientific research and teaching in the same degree while not all hospitals need to turn into three-in-one hospital, scientific research hospital or teaching hospital. It must be treated differently according to the actual situation.
Altitude ; Biomedical Research ; education ; manpower ; trends ; Humans ; Physicians ; psychology ; statistics & numerical data

Objective:To observe effects of Fuzheng Huoxue Recipe on blood growth hormone (GH) and insulin growth factor(IGF-1) in chronic renal failure(CRF) rats.Methods:Platt method was adopted to make 5/6 kidney excision CRF model rat,and the rats were divided into model group,Huangqi group,Enalapril group,Danshen group,Fuzheng Huoxue Recipe group and normal control group according to the blood creatinine level after modeling.Effects of Fuzheng Huoxue Recipe on GH and IGF-1 levels in CRF rats with large quantity of urinal protein were investigated.Results:GH and IGF-1 levels in the model control group were significantly higher than that in normal control group,and after treatment of Fuzheng Huoxue Recipe,blood GH and IGF-1 levels were significantly decreased.Conclusion:Fuzheng Huoxue Recipe can decrease blood GH and IGF-1 levels in CRF rats,and can delay advances of CRF.

BACKGROUND: To eliminate effectively and promptly sports fatigue and prevent excessive fatigue that are associate with the effects of training, improvement of sports results and health of athletes directly.OBJECTIVE:To explore the effects of acupoint iontophoresis on eliminating sports fatigue.DESIGN: Randomized controlled experiment was designed, in which, the athletes were taken as the objects.SETTING: Research and Teaching Room of Sport Medicine of Wuhan In stitute of Physical Education and College of Acupuncture and Massage of Shanghai University of Traditional Chinese Medicine.PARTICIPANTS:The experiment was performed in Wuhan Institute of Physical Education from June 2002 to June 2003. Totally 32 male athletes were selected as the objects,with similar level in training of kayak in Wuhan Institute of Physical Education. They were randomized into 5groups, named training group, training+ acupoint-dermal electric stimulation (dermal electric group), training + oral herbal administration (Chinese herb group), training + acupoint iontophoresis group (iontophoresis group)and training + Chinese herb + iontophoresis group(Chinese herb + iontophoresis group).There were 4 persons in the training control and 7 persons in the rest groups.METHODS:The experiment was performed in the training stage of large sports load in summer. The training methods were same in each group, totally for 8 weeks. ① Training group was taken as the control. ② In dermal electric group,after training,dermal electric stimulation was applied on shanzhong (CV 17), xinshu (BL 15), baihui (GV 20) and mingmen (GV 4).③ In Chinese herb group, after training, self-prepared shenwensan was applied for oral administration as decoction. ④ In iontophoresis group, after training, iontophoresis was used. ⑤ In Chinese herb + iontophoresis group,both self-prepared shenwensan and iontophoresis were applied.Trace observation of heart and lung functions were performed with subjective physical sensation scoring (before, after and on the 4th week of experiment), test of sports results,test of heart and lung functions and radioimmunoassay(RIA) (before and after experiment) in athletes.MAIN OUTCOME MEASURES: Subjective physical sensation scoring,test of sports results,test of heart and lung functions and determination of levels of endothelin (ET) and calcitoningene-related peptide (CGRP).RESULTS:The athletes in all of groups accomplished the training and tests of every index and the results had all entered final analysis.① Subjective sensation of athletes was all improved obviously and the effect of iontophoresis was improved significantly compared with dermal electric group and Chinese herb group (P ＜ 0.05). ② The results of rowing projects were improved to different extents in each group.The improved amplitude of 12 km training in every treating group was different significantly compared with training group (P ＜ 0.05). The result of 2 km training was improved most significantly in Chinese herb + iontophoresis group (P ＜ 0.05).③ VO2max was increased in Chinese herb group,iontophoresis group and Chinese herb + iontophoresis group to different extents. Maximal respiratory quotient (Rqmax)was in tendency of increasing in every group after experiment, HR-AT was all in tendency of decreasing and it was decreased in large amplitude in Chinese herb + iontophoresis group (P ＜ 0.05). The time of exhaustive sports was prolonged in all of groups,but the effect was the most obvious in iontophoresis group. ④ Endothelin (ET) was in tendency of increasing after experiment in each group, but the increasing amplitude was the largest in training group.The calcitoningene-relatedpeptide (CGRP) was in tendency of increasing in every group, but CGRP was increased with the best result in Chinese herb + iontophoresis group and that was with the least result in training group. The difference was no significant in comparisons among groups and before and after experiment(P ＞ 0.05).It was indicated that the training load of athletes had not reach the fatigue limitation.CONCLUSION:Cardiac sports fatigue is relevant to multiple factors.Acupoint iontophoresis can protect myocardium in multiple links and layers, improve cardiac function, reduce HR-AT, increase oxygenic endurance and promote the secretion of nervous peptide ET and CGRP; retard fatigue and promote fatigue elimination so as to delay cardiac sports fatigue.

[Objective]To discuss the effective method of idiopathic scoliosis and evaluate the result of screw-rod and screw-hook-rod system.[Method]From January 2000 to January 2004,85 cases with idiopathic scoliosis were treated by CD-Horizon instrument.Fifty-one cases were corrected with pedicle screws system only(group A),34 cases were applicated with pedicle hooks or vertebral plate hooks in thoracic vertebra,while with pedicle screws system in lumbar vertebrae(group B).The Cobb's angle of coronal plane and sagittal plane and horizontal plane malformation were compared in preoperative score,postoperative score and the score at 2 years follow-up.[Result]The mean followed-up duration of 85 cases was three years and a half.The coronal plane and sagittal plane correction rate of group A was higher than that of group B at postoperative and 2-year follow-up(P0.05).[Conclusion]Using CD-Horizon instrument is a very effective choice for patients with idiopathic scoliosis.Pedicle screw-rod system offers a significantly better curve correction compared with screw-hook-rod system.

Objective To understand the protection knowledge and practice to cope with common respiratory infection in senior middle school students in Chengdu ,and to provide basis for carrying out school health education and the establishment intervention model of respiratory infection .Methods A self-administered questionnaire was conducted among 2 237 senior one students in two senior middle schools in Chengdu .Results In terms of basic knowledge of common respiratory diseases ,47 .47% of the students had adequate total awareness scores .Respiratory diseases cognitive knowledge was not comprehensive enough .Especially for the under-standing of certain knowledge ,there was misunderstanding .The awareness rates for prevention of respiratory diseases and the treat-ment of behavior were high ,and they were 87 .54% and 71 .43% ,respectively .Conclusion The senior middle school students in Chengdu have some education demand of respiratory disease ,and schools should strengthen publicity and health education .

Objective To investigate the Reprimo and hMLH1 gene promoter methylation detection value in the diagnosis of early gastric cancer.Methods Chose patints in Shaanxi Provincial People’s Hospital from September 2013 to April 2014,50 cases of patients with chronic atrophicgastritis with intestinal metaplasia,50 cases of patients with gastricmucosal atypical hyperplasia,50 patients with gastric cancer,endoscopic gastric biopsy samples,and 30 cases of normal gastric mucosa biopsy tissues as control group.Analysis abnormal expression in Reprimo gene and hMLH1 genes promoter methylation,compared the differences.between groups of patients.Results The patients with gastric mucosatissues Reprimo and hMLH1 genes promoter methylation positive rate was significantly higher than that of normal group,the difference wasstatistically signifi-cant (P<0.05).Reprimo gene promoter methylation were:patients with chronic atrophic gastritis with intestinal metaplasia 28% (χ2 =10.18,P < 0.05).Patients with gastric mucosalatypical hyperplasia 56% (χ2 = 25.84,P < 0.05)and patients with gastric cancer 62% (χ2 = 30.36,P < 0.05).hMLH1 gene promoter methylation were:patients with chronic atrophic gastritis with intestinal metaplasia 20% (χ2 =4.39,P <0.05),patients with gastric mucosal atypical hyperplasia 44% (χ2 =15.13,P <0.05)and patients with gastric cancer 48% (χ2 = 17.41,P <0.05),high specificity of detection.Conclusion Reprimo and hMLH1 gene’s detect value in the diagnosis of early gastric cancer is very high,high specificity,it is an effec-tive way of diagnosis,treatment in clinical diagnosis of patients with broad prospects.

Objective To research the role of hypothalamocerebellar histaminergic projections in somatic‐visceral integration of rats .Methods A total of 120 SD rats were randomly divided to 6 groups :group A - F ;which separately microinjected saline (group A) ,triprolidine(selective antagonist of H1 receptor ,group B) ,2‐PyEA(selective agonist of H1 receptor ,group C) ,ranitidine (selective antagonist of H2 receptor ,group D) ,dimaprit (selective agonist of H2 receptor ,group E) and histamine (group F) to the deep nuclei of rats .The abilities of rats somatic‐visceral integration were observed when hypothalamocerebellar histaminergic pro‐jections were obstructed or enhanced compared to group A .Results The somatic‐visceral integration of rats were obstructed obvi‐ously in group D and enhanced in group E & group F compared to group A ,while group B and group C had no differences compared to group A .Conclusion Hypothalamocerebellar histaminergic projections play a key role in somatic‐visceral integration through the way of H2 receptors .

AIM: To investigate the indications and therapeutic effect of the conservative treatment and surgical treatment for vitreous hemorrhage.
METHODS: Hemorrhage reasons, degree of illness, and treatment and final results of vitreous hemorrhage were recorded from 2008 to 2013, and curative effect of different treatments was analyzed.
RESULTS:Selected 22 cases ( 24 eyes ) of conservative treatment, vision degree of 6 eyes ( 25%) had raised, 5 eyes ( 21%) occurred secondary retinal detachment without treatment, 3 eyes (12. 5%) suffered neovascular glaucoma were underwent operation, but the vision had lost completely. In 26 cases ( 31 eyes ) of control (operation) group, vision of 17 eyes (55%) had improved, 2 eyes (6. 5%) suffered the second operation, 2 eyes (6. 5%) suffered the third surgery, 3 eyes (10%) suffered eyeball atrophy. The number of ultimate vision improved with conservative treatment were significantly lower than the number of cases with surgical treatment (P<0. 05).
CONCLUSION: The surgical treatment is still visible vitreous hemorrhage treatment means of positive, but there will be eyeball atrophy and other serious complications, so we should choose a different methods in according to the different treatment objects and treatment time.

Pristimerin (Ⅰ), ?-sitosterol (Ⅱ),?-amyrin(Ⅲ),?-amyrin palmitate (Ⅳ)and 1-triacontanol acetate (Ⅴ)have been isolated from the root of Celastrus orbiculatus. I possesses strong cytotoxicity against P_(388) cell(IC_(50)=0. 267?g/ml )and LAX cell (IC_(50)= 0.018?g/ml)in vitro test. 13 CNMR assignments for I have been revised.