Objective: The aim of this study was to develop and assess the computer-assisted fabricated individual titanium mesh technique applied for reconstruction of fractured orbital walls to correct post-traumatic enophthalmus and diplopia. Methods: Twenty patients (12 males and 8 females) were inclu-ded in this study. All of patients suffered from unilateral impure orbital fractures. Group A included 8 patients who were reconstructed for the fractured orbit with computer-assisted fabricated titanium mesh technique. Group B had 12 patients treated with traditional methods (titanium meshes in 2, autogenous bone grafts in 7 and Medpor implantation in 3). CT data of group A was input into a image software to rebuild a 3-D orbit and then mirror the unaffected side onto the affected side to replace the demolished orbit. A resin model of the reshaped orbit was then produced and was used to develop custom-fabricated titanium mesh for repair of the fractured orbital wall. The fabricated mesh was used in operation to reconstruct the orbital wall. Treatment was assessed in comparison with traditional way by means of clinical examinations and CT measurements. Results: The difference between pre-operative and post-operative enophthalmos: the mean value of group A was 2.6 mm, group B was 2.0 mm;the difference between the pre-operative and post-operative enlargement volume of traumatic orbit: the mean value of group A was 4.3 mL, group B was 3.7 mL; Index of Medial orbital wall of both groups was 100% and Index of inferior wall reconstruction of group A was 84.7%, group B was 53.3%; The mean depth of implants of group A was 31.2 mm, of group B was 28.3 mm; Improvement of diplopia of group A was 1.5 quadrants, that of group B was 1 quadrant. There were no infection, gaze restriction, or graft extrusion in all patients after operation. Conclusion: Computer-assisted fabricated titanium mesh technique could provide more accurate orbital reconstruction and improve the correction of enophthalmus and diplopia.

Objective To investigate the effect of tacrolimus on the expression of nuclear factor-κB (NF-κB) in HaCaT cells stimulated by tumor necrosis factor-α(TNF-α),and on the expression of glucocorticoid receptor (GR)α and β in untreated HaCaT cells in vitro.Methods Cultured Ha CaT cells were treated with TNF-α(10μg/L) only,combination of TNF-α(10μg/L) and various concentrations (10-8mol/L, 10-7mol/L,10-6moL/L) of tacrolimus or tacrolimus of different concentrations only.After additional 12-,24-, 36- or 48-hour cnlture, Westem blot and immunofluorescenee-confocal laser scanning microscopy were used to detect the expressions of NF-κB,GRα and GRβ in HaCaT cells.Those untreated HaCaT cells served as the control.Results The relative protein expression level of NF-κB was increased in HaCaT cells after treatment with TNF-α for 24 and 48 hours zompared with untreated ceils (0.73±0.0316 and 0.8925±0.0171 vs 0.4988±0.03506,both P<0.05);however,the increase in NF-κB expression was inhibited by the combination treatment with tacrolimus,and the relative expression level of NF-κB protein was 0.6825±0.0263.0.6200±0.0163 and 0.5575±0.0299 in HaCaT cells treated with TNF-α plus tacrolimus of 10-8mol/L 10-7mol/L and 10-6mol/L,respectively;the difference was significant etween TNF-α-treated cells and those dealt with the combination of NF-α and tacrolimus of 10-7 or 10-6 mol/L (both P<0.05).No significant difference was observed in the expression of NF-κB by HaCaT cells between different time oints treated with tacrolimus of 10-8,10-7 or 10-6 mol/L.Also,there was no zignificant difference in the expression of GRα or GRβ between untreated HaCaT cells and those treated with tacrolimus of 10-8, 10-7 or 10-6 mol/L at any time point.Conclusions Tacrolimus ould inhibit the expression of NF-κB by TNF-α-stimulated HaCaT cells,but does not affect the expression of GRα or GRβ,in untreated HaCaT cells.

This paper discusses the meaning of the tutorial system on clinical practice;the qualifications,tasks and a responsibility of the tutors,expounds the role of tutors,and discusses the main problems of tutorial system of the undergraduate.

Objective To investigate the value of multi-slice spiral CT (MSCT) in diagnosis of colorectal carcinoma. Methods MSCT images of 42 cases of colorectal carcinoma proved by surgery and pathology were retrospectively analyzed. Multiplanar reconstruction (MPR) in all cases, and curved planar reconstruction (CPR), shaded surface display (SSD), CT virtual colonoscopy (CTVC) and Raysum were performed in some of the cases. MSCT findings were analyzed in comparison with operative and pathologic features. Results The detecting rate and quatitative rate of colorectal carcinomas with MSCT were 100%,respectively.The accurate rates were 82.9%(29/35) and 100%(7/7) in detection of invasion of surrounding structures and metastasis in abdominal organs respectively,the sensitivity and specificity for detetion of lymph node metastasis were 71%(22/31) and 100% with MSCT imaging.Conclusion MSCT scan with multiform reconstruction techniques can make definite diagnosis of colorectal carcinoma, which is helpful for treatment plan.

This paper aims to establish a new method of calibration and positioning in quantitative analysis of multicomponents by single marker (QAMS), using Shuanghuanglian oral liquid as the research object. Establishing relative correction factors with reference chlorogenic acid to other 11 active components (neochlorogenic acid, cryptochlorogenic acid, cafferic acid, forsythoside A, scutellarin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, baicalin and phillyrin wogonoside) in Shuanghuanglian oral liquid by 3 correction methods (multipoint correction, slope correction and quantitative factor correction). At the same time chromatographic peak was positioned by linear regression method. Only one standard uas used to determine the content of 12 components in Shuanghuanglian oral liquid, in stead of needing too many reference substance in quality control. The results showed that within the linear ranges, no significant differences were found in the quantitative results of 12 active constituents in 3 batches of Shuanghuanglian oral liquid determined by 3 correction methods and external standard method (ESM) or standard curve method (SCM). And this method is simpler and quicker than literature methods. The results were accurate and reliable, and had good reproducibility. While the positioning chromatographic peaks by linear regression method was more accurate than relative retention time in literature. The slope and the quantitative factor correction controlling the quality of Chinese traditional medicine is feasible and accurate.

Objective To optimize the processing conditions of Manchuiran Dutchmanspipe Stem with alkali. Methods The combination of radial basis function ( RBF) and response surface methodology ( RSM) was used to investigate the influence of NaHCO3 , concentration, duration and cycles of processing on the content of aristolochic acid. Results The optimal process was achieved when Manchuiran Dutchmanspipe Stem was soaked for 3 cycles in 0. 05 mol·L-1 NaHCO3 solution, for 24 hours in each cycle. The removal rate of total aristolochic acid approached to 83. 74%. Conclusion The combination of RBF and RSM provided a new method and good guidance for further toxicity attenuation for Manchuiran Dutchmanspipe Stem.

Objective To establish a UPLC method for detecting content of imperatorin and isoimperatorin in huoxiang zhengqi oral liquid. Methods ACQUITY UPLC BEH C18 (2. 1 mmí50 mm,1. 7 μm) was used and the mobile phase was methanol and water by gradient elution mode. The column temperature was 30 ℃ ,the flow rate was 0. 3 mL·min-1 and the detection wavelength was 248 nm. Results The linear range of imperatorin was 1. 305-13. 050 μg·mL-1 and the regression equation was as follow Y =13 633X+3 976 (r=0. 999 9). The linear range of isoimperatorin was 0. 596-5. 960 μg·mL-1 and the regression equation was Y=10 661X+1 073 (r=0. 999 9). The average recovery was 99. 25% (RSD=0. 74% ) and 98. 94%(RSD = 0. 63% ),respectively. Conclusion The method is accurate, rapid and reliable, and can be used to determine imperatorin and isoimperatorin in huoxiang zhengqi oral liquid.

【Objective】To observe therapeutic effect of Guishen Pill(GP) combined with acupuncture(AP) for male immune infertility(MII) and to investigate influence on acid phosphatase(ACP) in seminal plasma.【Methods】One hundred and five MII patients were equally randomized into three groups.Group A received GP combined AP,group B received GP and group C received prednisone.The therapeutic effect was evaluated in the three groups after treatment,and the changes of ACP were observed before and after treatment.【Results】The total effective rate was 91.43%,88.57% and 68.57%,and the cure rate was 45.71%,22.86% and 11.43% in groups A,B and C respectively.The difference of total effective rate was significant in groups A and B as compared with that in group C(P

Objective To study the levels of serum C -reactive protein (CRP),plasma fibrinogen (Fib),D-dimmer(DD)in the first onset young patients with acute progressive cerebral infarction.Methods 42 first onset young patients with acute progressive cerebral infarction(PIS group),50 cases of non -acute progressive cerebral infarction(N -PIS group)and 90 healthy people(health control group)were enrolled.The levels of serum CRP, plasma Fib and DD were detected and compared.Results PIS group:CRP (3.764 ±0.832)mg /L,Fib (3.994 ± 0.851)g/L,DD (1.560 ±0.225)μg/mL;N -PIS group:CRP (2.573 ±0.657)mg/L,Fib (2.468 ±0.739)g/L, DD (0.740 ±0.162)μg/mL;health control group:CRP (1.725 ±0.326)mg/L,Fib (2.103 ±0.584)g/L,DD (0.450 ±0.131)μg/mL.The levels of serum CRP,plasma Fib and DD of PIS group were higher than the other two groups(CRP:PIS group vs.N -PIS group t =8.89,PIS group vs.health control group t =13.99,N -PIS vs.health control group t =8.55,all P <0.01;D -D:PIS group vs.N -PIS group t =23.82,PIS group vs.health control group t =29.46,N -PIS group vs.health control group t =12.59,all P <0.01;FIB:N -PIS group vs.health control group t =2.85,P <0.05,PIS group vs.N -PIS group t =10.06,PIS group vs.health control group t =13.48,all P <0.01).Conclusion The levels of serum CRP,plasma Fib and DD are related to acute progressive cerebral infarction.

Objective To evaluate the treatment effect of Lobaplatin combined with Aidi Injection on patients with advanced NSCLC, and observe the influence on the level of serum VEGF by the treatment. Methods 60 patients, with advanced NSCLC and malignant pleural effusion, were divided into two groups (observation group and control group) randomly, with 30 cases in each group. All patients were treated with thoracentesis and inserted central venous catheter for the drainage of pleural effusion. After exhaustion of pleural effusion, the control group was treated with Lobaplatin by pleural perfusion, and the observation group was treated with Aidi Injection in addition to the treatment on control group. The treatment, side effects and quality of life and the level of serum VEGF were compared. Results After treatment, it is significant that clinical effects, side effect and KPS score of living quality of the observation group was better than that of the control group (P<0.05). The level of serum VEGF was significant lower than that before treatment in both groups (P<0.05). The decrease of VEGF serum level in the observation group was significantly lower than the decrease in the control group (P <0.05). Conclusion Lobaplatin combined with Aidi Injection was one of the effective method for the treatment of NSCLS with malignant pleural effusion, and was worthy of clinical application.