OBJECTIVE:To compared the efficacy and safety of doxofylline and aminophylline in the treatment of children with capillary bronchitis. METHODS:Totally 120 children with capillary bronchitis were randomly divided into observation group and control group. All children were given routine treatment,including oxygen inhalation,sputum suction and infusion supporting. Based on it,the observation group was treated by doxofylline 4 mg/kg adding into 15% glucose injection 50 ml by infusion,qd;control group was treated by aminophylline 4 mg/kg adding into 15% glucose injection 50 ml by infusion,bid. The course was 7 d. The capillary bronchitis severity scores,remission time of clinical symptoms,hospitalization time,utilization rate of glucocorticoid and the incidence of adverse reactions were observed. RESULTS:The total effective rate in observation group was significantly higher than control group,with significant difference(P<0.05). After 48 h and 72 h,the capillary bronchitis severity scores were significantly lower than before,and observation group was lower than control group after 72 h,with significant differences(P<0.05). The cough disappeared time and hospitalization time in observation group were significantly shorter than control group,and the utilization rate of glucocorticoid and the incidence of adverse reactions were significantly lower than control group,with signifi-cant differences(P<0.05). CONCLUSIONS:Based on the routine treatment,doxofylline has better efficacy and safety than amino-phylline in the treatment of children with capillary bronchitis.

AIM　To investigate protective effects and mechanism of tanshinones on ischemia-like injury models. METHODS　Six ischemia models including hypoxia, hypoglucose, oxidant injury, caffeine injury, nitric oxide neurotoxicity and excitatory amino acid injury were used to assay the anti-ischemic roles of tanshinones in cultured primary cortex neurons. The changes of injuried cortex neurons were observed by the way of morphological examination, and live neurons of crystal violet staining were measured according to absorbent index. RESULTS　It was found that tanshinones possessed obvious protective effects on primary neurons in injury models by the way of morphological examination. Crystal violet staining also indicated that tanshinones increased number of live neurons in injury models significantly. The protective effects of tanshinones on models of oxidant injury, caffeine injury and NMDA injury were superior to other injury models. CONCLUSIONS　83.0 μmol*L-1 tanshinones protected rat cortex cells from all injury models effectively in vitro.

AIM To investigate protective effects and mechanism of tanshinones on ischemia-like injury models. METHODS Six ischemia models including hypoxia, hypoglucose, oxidant injury, caffeine injury, nitric oxide neurotoxicity and excitatory amino acid injury were used to assay the anti-ischemic roles of tanshinones in cultured primary cortex neurons. The changes of injuried cortex neurons were observed by the way of morphological examination, and live neurons of crystal violet staining were measured according to absorbent index. RESULTS it was found that tanshinones possessed obvious protective effects on primary neurons in injury models by the way of morphological e~nation. Crystal violet staining also indicated that tanshinones increased number of live neurons in injury models significantly. The protective effects of tanshinones on models of oxidant injury, caffeine injury and NMDA injury were superior to other injury models. CONCLUSIONS 83.0 ?mol? L- 1 tanshinones protected rat cortex cells fm all injury models effectively in vitro.

Aim To investigate protective effects and mechanism of TPG on ischemia_like injury models. Methods Six ischemia models including hypoxia, hypoglucose, oxidant injury, calcium overload, nitric oxide neurotoxicity and excitatory amino acid injury were used to assay the anti_ischemic roles of TPG in cultured primary cortex neurons. Results It was found that TPG possessed obvious protective effects on primary neurons in injury models by the way of morphological examination. Crystal violet staining also indicated that TPG increased number of life neurons in injury models significantly. Couclusions 50～200 ?g?ml-1 TPG protected rat cortex cells from all injury models effectively in vitro.

BACKGROUND:Many in vivo and in vitro experiments indicate that implantation of exogenous basic fibroblast growth factor can significantly promote the process of bone formation, but the in vivo degradation of exogenous basic fibroblast growth factor affects the therapeutic efficacy.
OBJECTIVE:To observe the effects of basic fibroblast growth factor-transfected mesenchymal stem cells which transfected using molecular biology techniques combined with al ogeneic bone in the repair of critical-size bone defects in sheep.
METHODS:Al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells, bone marrow mesenchymal stem cells combined with al ogeneic bone material stents, al ograft bone material,β-tricalcium calcium material were respectively implanted into critical-size bone defects in sheep. After 4, 8 and 12 weeks, histological and immunohistochemical staining was performed.
RESULTS AND CONCLUSION:At 12 weeks after implantation of al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells as tissue engineering bone, there were many cartilage-like structures in the operative binding region and a large amount of osteoblast-like cells in the center of operative region, and there was more material degradation in the entire operative area as compared with other groups;there were fibrous connective tissues ful of the pores, and osteoclast-like cells were commonly seen around the implant material;bone sialoprotein and col agen type Ⅰ expression were strongly positive. In the other three groups, although the cartilage-like structure appeared in the binding region, dead bone structure was found in the central area, and bone sialoprotein and type Ⅰ col agen expression was weak. These findings indicate that al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells can basical y repair critical-size bone defects in sheep.

AIM To study the flavonoids from Morus nigra L.leaves.METHODS The n-butanol fraction of 80％ ethanol extract from M.nigra leaves was isolated and purified by macroporous adsorption resin and preparative HPLC column,then the structures of obtained compounds were identified by spectral data.RESULTS Eleven compounds were isolated and identified as quercetin (1),icariside Ⅱ (2),rhodionin (3),rhodiosin (4),quercetin-3-O-β-(2-O-acetyl) galactopyranoside-7-O-α-arabia pyran glucosides (5),salidroside (6),kaempferol-3-O-β-(2-O-α-L-rhamnopyranosyl)-glucuronide (7),quercetin-3-O-α-rhamnopyranosyl-(1 →2)-β-glucopyranosiduronide (8),rhamnetin-3-O-galactoside (9),quercetin-3'-O-β-glucoside (10),quercetin-3-O-β-D-glucopyranoside (11).CONCLUSION Compounds 1-10 are isolated from this plant for the first time.

Objective To validate the analytical performance of four LP(a)reagents with Immunoturbidimetry method used on the automatic biochemistry analyzer for preliminary clinical application.Methods The performance of four LP(a)reagents (labeled as A,B,C,D)with method from RANDOX,Zhejiang Kuake Co.,Beijing Leadman Co.and Beijing Jiuqiang Co.on Olympus AU5800 automatic biochemistry analyzer were assessed.The precision,linearity range,accuracy,disturbance (vita-min C,bilirubin,hemoglobin,TG)were assessed.Results The within-run CVs of the four reagents (A,B,C and D)were 0.64%~1.18%,3.59%~4.75%,1.33%~3.05% and 1.43%~2.01% respectively.The between-run CVs in A,B,C and D were 1.04%~1.7%,3.81%~4.93%,2.16%~4.76% and 2.33%~3.21% respectively,lower than the stated.The lin-earity range was 82~923 mg/L (r2 =0.997),130~935 mg/L (r 2 =0.996 4),120~1025 mg/L (r 2 =0.992 1)and 117~943 mg/L (r2 =0.999 5)in the four reagents,which demonstrated a sound linear correlation.For interference tests,no re-markable interferences (<±10%)of reagent A and reagent D were detected when Vitamin C≤10 mg/dl,hemoglobin≤200 mg/dl,bilirubin≤40 mg/dl and TG≤500 mg/dl.Interference of reagent B was found when VC≥5 mg/dl,TG≥250 mg/dl and when TG≥250 mg/dl reagent C was interfered significantly.The four LP(a)reagents were used to detect the lipid con-trol,and the deviations of the target value were - 8.07%,1.34%,- 8.05% and 7.38% respectively.Conclusion When used in automatic biochemical analyzer,the four LP(a)reagents showed high precision.The four reagents are all able to meet clinical test requirements,nevertheless,anti-interference capability were different.

BACKGROUND: In the researches of Ginkgo Biloba Extract(GBE) in the treatment of cerebra ischemia, because of the application of generally anaesthesia medication that may induce the alteration of cerebral temperature, the accuracy of the results may be affected.OBJECTIVE: To investigate the impact of domestic GBE on antioxidase and lipid peroxide of cerebral ischemic reperfusion tissue as well as the water content of ischemic brain tissue under normothermia.DESIGN: A randomised controlled trial.SETTING and MATERIALS: Study was conducted in the Tongji Medical University of Huazhong Science and Technology University. A total of 24 Wistar rats with a mass from 250 g to 300 g were randomly allocated into sham-operation group ( n = 8 ), cerebral ischemia control group ( n = 8) and cerebral ischemia GBE treatment group( n = 8) . The animal model of normothermia rat with left middle cerebral artery ischemia for 2 hours and reperfusion for 2 hours was prepared with the reference of Nagasawa method in the animals of control group and treatment group for contrast study.INTERVENTIONS: The cerebral temperature of the rats was reflected by the temperature of the temporal muscle. The temperature-measuring probe was embedded into the deep part of the left temporal muscle closed to osseous ectoblast. The temperature was continuously monitored by semiconductor oxide temperature sensor. The temperature of the head was heated with 60 W filament lamp and adjusted by automatic double-controlling craniocerebral cooling instrument to maintain the cerebral temperature at normothermia level of 36.5 ℃ - 37 ℃. The normothermia cerebral ischemic reperfusion injury rat model was established according to the design. GBE injection was injected respectively into abdominal cavity in the rats of cerebral ischemia GBE treatment group at the following time point: 12 hours, 8 hours and 4 hours before operation, immediately after cerebral ischemia and immediately after reperfusion, with 3 mL each time and 5 times in total. Same times and dose of normal saline was injected into the abdominal cavity in the rats of both control group and sham-operation groups.MAIN OUTCOME MEASURES: Superoxide dismutase (SOD), glutathione peroxidase(GSH-Px), reduced glutathione(GSH), malondialdehyde(MDA)and water contents in the cerebral ischemic tissue.RESULTS: The levels of SOD, GSH-Px and GSH in cerebral ischemia control group were(73.35 ± 12. 86) NU/mg, (167.37 ±54.34) μkat/g and (196. 84 ± 22.75) μg/g respectively, which significantly lower than that (96. 02± 16. 83) NU/mg, (338.57±84.02) μkat/g and(337.51± 34. 89) μg/g of sham-operation group( P ＜ 0. 01 or P ＜ 0.05) . The SOD, GSH-Px and GSH levels of cerebral isehemia GBE treatment group were (87.24± 15.03) NU/mg, (316. 56 ±93.52) μkat/g and(263.16±28.54) μg/g, which significantly higher than that of cerebral ischemia control group(P ＜ 0.01 or P ＜ 0.05) .The MDA level of cerebra ischemia control group was (308.34 ± 26.81 ) nmol/g, which significantly higher than that(101.46 ± 10.97) nmol/g of sham-operation group( P ＜ 0.01 ) .The MDA level of cerebral ischemia GBE treatment group was(125.86± 13.90) nmol/g, which was significantly lower than that of sham-operation group ( P ＜ 0.01 ) . The water content of cerebral ischemia control group was(80. 45 ± 0.44)%, which was significantly higher than that (78.20 ± 0. 25 ) % of sham-operation group ( P ＜ 0.01 ) . The water content of cerebral ischemia GBE treatment group was(79.63 ± 0.46) %, which was significantly lower than that of cerebral ischemia control group( P ＜ 0.05).CONCLUSION: Domestic GBE can inhibit the excessive production of free radicals and the lipid peroxidation during cerebral ischemia and reduce cerebral oedema and the destruction of blood-brain barrier to protect cerebral ischemic tissues under cerebral normothermia.

Objective To study the enantioselective characteristics and laws of ?-adrenoceptor antagonist isomers on Chiralcel ○R OD column. Methods The experiments were performed under the following HPLC conditions: a Chiralcel OD column (250 mm?4.6 mm, 10 ?m) as analytical column, a mixture of n-hexane/2-propanol/ triethylamine as the mobile phase, the fluorescence detection wavelengths at 275 nm (? ex) and 310 nm (? em), and the flow rate at 0.5 mL?min -1. The effect of interaction between stationary phase and 2-propanol or triethylamine concentration in mobile phase on the enantiomer resolution were investigated by the stoichiometric displacement model for retention. Results The lgI values of R-enantiomers of 3 out of 5 ?-adrenoceptor antagonist were higher than those of S-enantiomers. The 2-propanol in mobile phase would differently affect the resolution between enantiomers for each ?-adrenoceptor. With the increase of triethylamine concentration in mobile phase, the capacity factor (k′) of the enantiomers decreased but the resolution increased. Conclusion The lgI and Z values of SDM-R could be employed to characterize the resolution efficiency of Chiralcel OD column and the specific effect of enantiomers interacted with the stationary phase. Adding triethylamine in mobile phase will increase the resolution efficacy and changing column temperature can promote enantiomer resolution.

0.05 ); the positive percentage of IGFBP-2 was statistically higher in breast cancer with metastasis in lymph node than those without metastasis in lymph node (P 0.05 ,r_s= 0.271 ). CONCLUSION: The expression of IGFBP-2 may play an important role in the progression, invasion and metastasis of breast cancer, and there is a positive correlation between the expression of IGFBP-2 and ER in breast cancer. It demonstrates that over expression of IGFBP-2 may be a reference to evaluate unfavourable prognosis of breast cancer.