Objective To research a pure capacitance sensor for acquisition of cardiac phonocardiogram. Methods Under the phantom power supply (48V), the faint vibration signal of the heart was processed into electric signal by larger pre - treatment circuit module, shielded cable and the sensor pre-amplifier of circuit module. Results With some experiments, it can be proved that the sensor won't have affect on the sound field which frequency band under 1 000Hz and can detect the level of sound field like 20dB:30dB and 150dB or higher. Conclusion So with the sensor, the precise signal which can exactly reflect the status of patients' hearts can be detected, and it suits to work in complex condition for its high sensitivity, wide frequency band and low distortion.

Objective To explore the influence of salvianolic acid B(SalB) to blood brain‐barrier(BBB) in vitro oxygen/glu‐cose deprivation and reintroduction .Methods We used the human brain microvascular endothelial cells and C6 cells to build BBB model that in vitro ,and the models was divided into normal groups ,experiment groups ,treatment groups .The Earle′s liquid and the condition of 93% N2 ,2% O2 ,5% CO2 replaced the normal culture environment to simulate environment of oxygen /glucose depriva‐tion and reintroduction .The changes of BBB permeability was detected by the horseradish peroxidase permeability test .The tight junctions(TJs) was detected by immunofluorescence and electron microscope .Results The permeability of treatment group was significantly lower than the experiment group (P < 0 .05) ;The TJs of normal group was continuous and density ;the experiment group′s TJs was interruption and osteoporosis ;and the observation of treatment group′s TJs was between of the two group above . Conclusion The SalB can directly protect the BBB during oxygen /glucose deprivation and reintroduction ,and decrease the permea‐bility .The investigation also provided the research foundation for the SalB to protect the BBB .

Objective To analyze clinical pathological characteristics of gastric cancer in elderly people,and to study the characteristics of gastric cancer incidence.Methods A retrospective analysis of clinical data of gastric cancer in elderly people which were diagnosed by gastroscopy and pathology from 2003 to 2008 was undertaken,and compared to it in young people at the same period about gender,clinical symptoms,lesion,pathological types.Results There were 70 cases of men(77.8%)and 20 cases of woman(22.2%)in elderly group,but there were 3 cases of man(25.0%)and 9 cases of woman(75.0%)in young group,there wsa significant difference between two group(P ＜0.01);The first symptoms in elderly group and young group were loss of appetite(50 cases,55.6% and 1 case,8.3%),thin and weak(38 cases,42.2% and 1 case,8.3%).difficulty swallowing(23 cases,25.6% and o case),anehia(29 cases ,32.2% and 2 cases,16.7%),upper abdominal pain(34 cases,37.1 % and 4 cases,33.3%)re spedtively.There was no significant difference between two group(P ＞ 0.05).The lesion of gastric cancer in two group were funds of stomach and cardia(39 cases,43.3% and 1 case,8.3%),antral(26 cases,28.9% and 7 cases,58.3%).body of stomach(22 cases,24.4% and 4 cases,33.3%).entire stomach(3 cases,3.3% and 0 case)respectively.There was significant difference between two group(P ＜0.05).The main of pathological types was abeno carcinoma.and the main in elderly group was moderate,high differentiation(85%),and the main in young group was moderate,low differentiation(59%).There was significant difference between two group(P ＜ 0.05).Conclusions The symptoms of gastric cancer in elderly people was non-specific,and it had more complications.We found that early diagnosis of gastric cancer in elderly people was difficult and it had poor prognosis.

Objective To discuss the clinical effect of transurethral cystitis glandularis electrocision combined with treating the diseases under bladder outlet in female.Methods One hundred and two female cases of cystitis glandularis who received transurethral electrical resection of the bladder mucosa combined with treating the diseases under bladder outlet were selected as experimental group,and another 78 female cases were selected as control group,who only received transurethral electrical resection of the bladder mucosa.The visual analogue score (VAS) was observed before and after a 12-week treatment,and the efficacy was evaluated by analysis of non parameter RIDIT.Results The operation time in experimental group and control group were 15-40 (26 ± 13) min and 8-19 (13 ± 5) min,respectively,and there was significant difference between two groups (P ＜ 0.05).Two groups cases urinated smoothly after the removal of catheter,without the occurrences of urinary fistula and incontinence.During the 12-weeks of follow-up,44 cases were cured,31 cases improved,15 cases were effective and 12 cases unimproved in experimental group; 26 cases were cured,21 cases improved,16 cases were effective and 15 cases unimproved in control group.There was significant difference between two groups (R =0.576,95％ CI0.519-0.633,P＜ 0.05).Conclusion Although the operation time is lengthened by transurethral cystitis glandularis electrocision combined with treating the diseases under bladder outlet in female,this method obviously improves curative effect.

OBJECTIVETo explore the effect of the expanded capsule on biomechanics of the transplanted autologous costal cartilage.

METHODSTen New Zealand white rabbits aged 3 months were involved and four 15 ml tissue expanders were implanted symmetrically on the back of each rabbit. After 1 month, expanded capsules were removed (experimental group) on the left side and were reserved (control group) on the right side. Meanwhile, the 6th and 7th costal cartilage without the perichondrium were taken out and each cartilage was divided into two pieces and put under the expanded capsule symmetrically. The cartilage at the upper sides and lower sides were taken out at 4 weeks and 8 weeks later, respectively. All the cartilages were sculptured for the demand of the machine text. The tension and compression property of the cartilages were assessed by the material testing machine (Instron 5967) and the results were analyzed statistically.

RESULTS8 weeks lateral, the average ultimate tension strength was (4. 954 + 2. 8) MPa in experimental group and (2.939 + 0.842) MPa in control group. The average compressive strength was (58.74 ± 13.77) MPa in experimental group, and (47.61 ± 11.41) MPa in control group. The biomechanics property of the cartilages in the experimental group is better than the control group, and the differences were statistically significant.

CONCLUSIONSRemoving the expanded capsule is benefical to maintain the biomechanics of autologous costal cartilage.

Animals ; Autografts ; Biomechanical Phenomena ; Costal Cartilage ; physiology ; transplantation ; Humans ; Rabbits ; Ribs ; Tissue Expansion ; Tissue Expansion Devices

Objective To study the immuno-protective effect against Schistosoma japonicum challenge of the positive monoclonal phages which were screened from the 12 mers-phage random peptide library by the new model rabbit serum. Methods The new model was established by injecting the Schistosoma japonicum infection rabbits with inhibitor of phenol oxidose. Positive clones immunoscreened with the new model rabbit sera were absorbed by SEA immune rabbit sera, and 14 clones selected randomly from them were compared and their antigenic ability was identified by ELISA. The two best positive monoclonal phages (No.8, No.13) recognized by the new model rabbit sera were selected to immunize Kunming mice by subcutaneously injecting 1?10~15 pfu positive phages at 0, 2nd, 4th week respectively. After 4 weeks of the last immunity,each mouse was challenged with 40?1 S.japonicum cercariae. All mice were sacrificed after 42 days and the reduction rates of adult worms and the liver eggs were investigated. Results The positive phage clones after immune absorption were weakly recognized by the SEA immune rabbit sera. The 14 monoclonal phages were recognized by the rabbit sera of the new model and the normal model. Especially No.8, No.13 were strongly recognized by the rabbit sera of the new model,while weakly recognized by the SEA immune rabbit sera. The reduction rates of adult worms and liver eggs induced by the monoclonal phage No.13, the monoclonal phage No.8 and the original peptide library were 35.81% and 63.32%, 32.09% and 52.02%, 14.90% and 30.64%, respectively. Conclusion Most clones of simulated epitopes of SEA can be removed by absorbing positive clones with SEA immune rabbit serum .The 14 monoclonal phages from the new model contain the simulated S.japonicum epitope. The two monoclonal phages have higher reduction rates of adult worms and eggs than original 12 mers-phage random peptide library and the positive polyclonal phages.[

OBJECTIVE:To establish a rapid,accurate and standardized DNA barcode identification method for the ferulic me-dicinal plants. METHODS:Genomic DNA was extracted from Ferula sinkiangensis K. M. Shen and Ferula fukanensis K. M. Shen,ITS2 sequences were amplified and sequenced,analytical similarity search method was conducted,13 species 26 samples gene ITS2 sequences of ferulic medicinal plants in GenBank database were downloaded and comparatively analyzed;interspecies and intraspecies genetic distances were calculated,and phylogenetic tree was developed for cluster analysis. RESULTS:According to calculation,species genetic distances of the 15 species ranged 0.009-0.230,average genetic distance was 0.018;results of clus-ter analysis showed DNA barcode ITS2 sequences can cluster the 32 kinds of ferulic medicinal plants into different classes. CON-CLUSIONS:The established DNA barcode ITS2 sequences identification method can accurately and rapidly identify the genuine and false samples of ferulic species.

1. The blood supply of the tensor fascia lata myocutaneous free flap comes chiefly from the ascending branch of the lateral circumflex artery (76.74％) or its ascending and the transverse branches (23.26％). In most cases the ascending branch may be served as the vascular pedicle of myocutaneous free flap in transplatation. In a few cases the transverse branch my be used instead of the ascending one.2. The projection of the origin of the lateral circumflx artery on the body surface is at a point averaging 96.5mm below, and 49.2mm medial to the anterior superior iliac spine. This point is the surface landmark of the lateral circumflex artery. 3. The branches of the lateral circumflex artery may be classified into four types. Type 1—32 cases, 74.42％; Type Ⅱ—3 cases, 6.98％; Type Ⅲ—7 cases, 16.28％; Type Ⅳ—1 cases, 2.33％.4. The tensor fascia lata is chiefly supplied by the ascending branch of the lateral circumflex artery, it divides into a short superior and a long inferior twigs. Both twigs enter the muscle and form abundant anastomoses in the subcutaneus tissues. In order to reduce the thick ness of the free flap for the use in plastics, the superior twig with its supplying muscle is cut away and the inferior one's left to supply myocutaneus flap.5. The angle between the ascending branch and the inner surface of the tensor fasca lata varies between 44? to 120?, with an average of 66.5?. The original angle must be kept in transplantation, which favours the survival of the myocutaneus free flap.

Objective:To evaluate the expression of apoptosis related gene Fas Ligand(FasL) in human hepatocellular carcinoma(HCC) cells HepG2 and its significance in apoptosis.Methods:The recombinant eukaryotic expression plasmid pcDNA3.1hisB- FasL was transfected into HCC cells HepG2 by lipofection, and then soluble FasL was examined in the supernatant of culture cells by EIA, FasL expression in HepG2 cells were detected by immune histochemistry. After stained by annexin V and propidium iodine, cells were passed throw flow cytometer and examined by fluorescence microscope and sym-focus laser scaning microscope.Results:In comparison with untransfected cells,the soluble FasL could be detected in the supernatant of transfected cells, Fas L can be expressed in the membrane and cytoplasm of transfected cells. The apoptotic cell rate in transfected cells was 36.30%, as the control, untransfected cells was 11.53%.Moreover, the different stage of apoptotic cells could be distinguished by annexin V and propidium iodine stain.Conclusion:This supports a novel pathway of HCC cells were apoptotic itself via the CD95-CD95 ligand system without involvement of immune cells.

Background As a new tonometer,it is necessary to assess the clinical value of Icare rebound tonometer.Objective This study was to compare the intraocular pressure(IOP)values measured by Icare with that measured by GAT,and discuss the clinical value of leare rebound tonometer. Methods IOP measurement was performed on 152 eyes of 78 subjects with suspicious glaucoma,glaucoma,refractive error and normal examinnee by Icare and GAT respectively.The Icare IOP was measured firstly and then the GAT IOP was carried out with the 3-or 5-minute interval.The IOP values were compared between Ieare and GAT.This study was approved by Ethic Committee of Wuhan General Hospital of Chinese PLA.Written informed consent was obtained from each subject prior to this study. Results The mean IOP values of Icare and GAT were(19.16±5.03)mmHg and(18.41±4.52)mmHg respectively.The differences between Icare IOP and CAT IOP were less than or equal to 1 mmHg in 96 of 105 eyes(63.2%).The positive correlation was found between the Icare IOP and GAT IOP(r=0.940,P<0.01).The Ieare IOP was lower than that of GAT when IOPIcare<16 mmHg,however,the IOP of Icare were higher when IOPIcare≥6 mmHg;the IOP of Icare were higher than that of GAT in the total CCT range.The correlation coefficients of IOP of Icare or CAT with CCT were 0.341(P<0.01)and 0.333(P<0.01),respectively. Conclusion Compared with GAT,Icare is more feasible in clinic because it is practicable and reliable.