OBJECTIVE:To investigate the characteristics of adverse drug reactions(ADR)of the hospitals(Level 2 and above) of Wuxi in order to promote rational use of drugs in the clinic.METHODS:2 473 cases of ADR collected from Jan.2005 to Dec.2006 in 11 hospitals(Level 2 and above)of Wuxi were retrospectively studied.RESULTS:Most patients who had ADR were old patients over 60 years and teenagers(≤10years).Most ADR were caused by anti-infection drugs(60.9%),followed by the preparations of Chinese materia medica(13.7%).The intravenous medication was the main route of medication resulting in serious adverse reactions;Most of the ADR cases were known and mild, 74 cases were severe and scarce(3.0%).CONCLU-SION:More attention should be paid to monitoring clinical ADR and the quality of ADR reports needs to be improved.

Deubiquitylases remove ubiquitin moieties from different substrates to regulate protein activity and cell homeostasis .Since this posttranslational modification plays a role in several different cellular functions ,its deregulation has been associated with different pathologies .Aberrant expression of Ubiquitin -Specific Peptidase 22(USP22)has been associated with poor cancer prognosis .This article reviews the research status of USP 22.

Objective　To evaluate the safety and efficiency of percutaneous coronary interventions(PCI) in octogenarian Chinese with coronary artery disease.　Methods　A total of 43 patients aged over 80 years old underwent 45 such procedures at Queen Mary Hospital from October 1994 to May 1998. There were 27 male and 16 female 〔 mean age (82.3±2.5), range (80-89)〕. 55.8% of these patients had multi-vessel lesions. A total of 48 lesions in 43 patients was treated 〔25 LAD(52.1%), 11 LCX(22.9%) , 11 RCA(22.9%), 1 LM (2.1%)〕. 　Results　Thirty-seven (86.1%) patients were successfully performed PCI. Sixteen of them deployed stenting. Among the 6 lesions which failed to cross, 5 of lesions were LAD, 3 of them were total occlusion. The success rate and complication during the procedure were not significant difference compared with the control in 237 patients ＜80 years of age. At 18 months follow up, 78.5% of these patients was free or much improved of chest pain. The restenosis rate was 36.0% confirmed by coronary angiogram performed in 25 patients. 　Conclusions　In octogenarians with coronary artery disease PCI was a safe and effective procedure with a high successful rate, low acceptable complication rate and a favorable immediate and long term outcomes.

Objective To evaluate the effect of PTCA in patients younger than 40 years of age. Methods Patients aged 40 years or less who underwent PTCA were recruited. Clinical and angiographic data were reviewed. Results A total of 82 patients underwent such procedures. Successful PTCA were performed in 80 patients (97.56%), 93 lesions were treated (97.89%), resulting in an improvement in diameter stenosis from 84.5%?11.8% to 4.1%?1.2%. One patient (1.22%) required bypass surgery without death and Q-wave myocardial infarction. During 42.1?6.8 months′ follow-up, 3 patients died (non-cardial cause in two cases), 3 sustained a myocardial infaraction, and 1 required bypass surgery. 87.8% of patients were free of angina. The target vessel revascularisation rate (TVR) was 12.19%. Angiographic follow up were performed in 70 patients (8.4?5.2 months). The total restenosis rate was 31.43%, 13.16% in stent group. Conclusion Coronary angioplasty has a high immediate success rate and good long-term result when performed in young patients who can be treated safely and effectively with coronary interventions.

Objective To study the clinical features and treatment on hepatocellular carcinoma(HCC) with obstructive jaundice as primary symptom. Method We collected 39 cases of patients of HCC with obstructive jaundice as primary symptom in our hospital,the clinical features and treatments were studyed.Results The incidence of such patients was 6.3% in all HCC patients and all patients were operaed;the positive diagnostic rate of B ultrasonud and T was 88.2% and 80% respectively ,it was 48.7% in cases with intraportal thrombi.Conclutions Preperative correct diagnosis depends on image test,hepatectomy with biliary duct drainage is the mainstay for treatment .

Objective To investigate the effect of PEP-1-heme oxygenase-1 (PEP-1-HO-1) fusion protein transduction on hypoxia-reoxygenation (H/R) injury in rat H9c2 cells. Methods After construction of the prokaryotic expression plasmid pET15b-PEP-1-hHO-1 containing the human heme oxygenase-1 gene, it was then transformed to make PEP-1-HO-1 fusion protein express. The H9c2 cells were cultured in high-glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum and randomly divided into 4 groups (n = 4 each): control group (group C), H/R group, low-concentration fusion protein group (group L-HO), and high-concentration fusion protein group (group H-HO). The cells were exposed to 22 h of hypoxia followed by 8 h of reoxygenation. PEP-1-HO-1 fusion protein was added to the culture medium with a final concentration of 1.0 μ mol/L (group L-HO) or 2.0 μmol/L (group H-HO) before hypoxia. The cells and supernatant of the culture medium were collected after reoxygenation to determine the activity of lactate dehydrogenase (LDH) in the supernatant and the content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in the cells. Results The SOD activity was significantly lower, while the MDA content and LDH activity were significantly higher in group H/R, L-HO and H-HO than in group C (P ＜0.05). The SOD activity was significantly higher, while MDA content and LDH activity were significantly lower in group L-HO and H-HO than in group H/R, and in group H-HO than in group L-HO ( P ＜ 0.05). Conclusion PEP-1-HO-1 fusion protein transdution can protect H9c2 cells against H/R injury in rats.

China ; Occupational Exposure ; Threshold Limit Values ; Ultraviolet Rays

Medicinal materials research and development of new drug of traditional Chinese medicine (TCM) research is the premise and foundation of new drug research and development, it throughout the whole process of new drug research. Medicinal materials research is one of the main content of the pharmaceutical research of new drug of TCM, and it is also the focus of the new medicine pharmaceutical evaluation content. This article through the analysis of the present problems existing in the development of TCM research of new drug of TCM, from medicine research concept, quality stability, quality standard, etc are expounded, including medicine research idea value medicine study should focus on the important role and from the purpose for the top-level design of new drug research problem. Medicinal materials quality stability should pay attention to the original, medicinal part, origin, processing, storage, planting (breeding), and other aspects. Aspect of quality standard of medicinal materials should pay attention to establish the quality standards of conform to the characteristics of new drug of TCM. As the instruction of TCM new drug research and development and the scientific nature of the review, and provide the basis for medicinal material standards.
Biomedical Research ; methods ; standards ; China ; Drug Stability ; Drug Storage ; Drugs, Chinese Herbal ; chemistry ; standards ; Medicine, Chinese Traditional ; methods ; standards ; Quality Control

Objective To understand the protection knowledge and practice to cope with common respiratory infection in senior middle school students in Chengdu ,and to provide basis for carrying out school health education and the establishment intervention model of respiratory infection .Methods A self-administered questionnaire was conducted among 2 237 senior one students in two senior middle schools in Chengdu .Results In terms of basic knowledge of common respiratory diseases ,47 .47% of the students had adequate total awareness scores .Respiratory diseases cognitive knowledge was not comprehensive enough .Especially for the under-standing of certain knowledge ,there was misunderstanding .The awareness rates for prevention of respiratory diseases and the treat-ment of behavior were high ,and they were 87 .54% and 71 .43% ,respectively .Conclusion The senior middle school students in Chengdu have some education demand of respiratory disease ,and schools should strengthen publicity and health education .

Objective To determine whether the fixation method of electrode of the improved pediatric video electroencephalogram (VEEG) is better than the traditional fixation method. Methods A total of 746 children patients who needed VEEG monitoring were divided into the experimental group and the control group with random digit table with 373 patients in each group. Improved fixation method and traditional fixation method of electrode were adopted during VEEG monitoring in the experimental group and the control group. The advantage and disadvantage of the two fixation method were compared through evaluation of skin injury of scalp and deltoideus triangularis, electrode loss and electrode breakage. Results The incidence of skin injury of scalp and deltoideus triangularis, electrode loss and electrode breakage in the experimental group were 7, 0, 29, and 12, which were less than 29, 6, 71, and 37 of the control group, the differences were of statistical significance (χ2=14.126, 20.371, 13.652, P<0.01 or 0.05). Conclusions Improved electrode fixation method during pediatric VEEG can avoid skin injury of scalp and deltoideus triangularis, reduce electrode loss and electrode breakage, besides, it also can reduce medical cost and discomfort of children. The modified electrode fixation method proved to be superior to the traditional method and it is worth popularization and application.