1.Comparison of multi-slice helical CT cholangiography and MR cholangiopancreatography in diagnosis of pancreaticobiliary obstructive diseases
Keyang WANG ; Xin DONG ; Wen HE
Chinese Journal of Medical Imaging Technology 2010;26(3):521-524
Objective To compare the diagnostic value of 64-slice helical CT cholangiography and MR cholangiopancreatography (MRCP) for pancreaticobiliary obstructive diseases. Methods Thirty-six patients with pathologically proved pancreaticobiliary obstruction or endoscopic retrograde cholangiopancreatography (ERCP) were examined with MRCP and routine enhanced CT scanning. CT row data of portal venous phase were reconstructed with 0.625 mm thickness and intervals. Then multiplanar reformation (MPR) of intra- and extrahepatic biliary duct, gallbladder and pancreas was generated, and curved planar reformation (CPR) was performed when necessary. The accuracy of MPR (and CPR) and MRCP in evaluating the site and nature of obstruction was compared. Results The accuracy of MPR and MRCP was 97.22% and 94.44% in evaluating the site of obstruction, respectively. In evaluating the nature of obstruction, the accuracy of MPR and MPCP was 83.33% and 80.56%, respectively, and the accuracy of MPR increased to 88.89% in combination with CPR in some patients. There was no statistical difference between the accuracy of MPR and MRCP in evaluating the site and nature of obstruction, while their diagnostic consistency was medium (Kappa=0.471). Conclusion Both MSCT cholangiography and MRCP have high diagnostic value in pancreaticobiliary obstruction, while the former gets some advantages in images review for clinicians.
2.Study on the competence of master of public health
Shihao WEN ; Weimin REN ; Fang XU ; Dong ZHOU ; Xiang HE
Chinese Journal of Medical Education Research 2013;(10):989-992
A questionnaire was used to survey the perspective of the 22 mentors,24 students, 27 graduate students and 27 public health units on the competence of MPH student. The result showed that the practice skill,analyzing skill,program plan and practice skill,and emergency reaction abil-ity got more attention. This suggested that to the training of MPH,the training objective should focus on the ability to resolve problems; the curriculum should focus on the characters of public health and on the practice training.
3.Isolation and Screening of Endophytic Antifungal Bacteria from Mangroves
Zhen-Ming CHEN ; Jin-Jian HE ; Hong HE ; Xing-Feng ZHANG ; Wen-Dong SONG ;
Microbiology 1992;0(03):-
The endophytic bacteria of the mangroves were studied in this paper. The results show that there are 1.728 (0. 195 -4.225)?104cfu/g (fw) bacterial endophytes in the variety of mangroves, the most population of the endophytic bacteria was found in Rhizophora stylosa, the figure was4. 225?104cfu/g (fw) , the next was Bruguiera gymnorrhiza, Aegiceras corniculatum, Kandelia candel and Avicennia marina. In parts of the mangroves, the contents of the bacteria in stem was the most, the figure was 1. 649?10 cfu/g (fw) , then the root and the leaf. Of the bacteria, about 43. 53% strains expressed the antagonism against the growth of the plant pathogens, such as Fusarium oxyspontm f. sp. cubense , Colletotrichum sp. and Rahtonia solanaceance etc. and these bacteria were identified as Bacillus sp. . The results also showed that 9 of the 13 strains (69. 23% ) could promote the growth of the tomato, while 4 strains (30. 77% ) restrained the tomato's growth.
5.Expression of Beclin1, LC3 and mTOR and their significance in colorectal carcinoma
Shuhua WU ; Jinlong HU ; Shuang HE ; Feifei WEN ; Chenbo SUN ; Dong TIAN
Chinese Journal of Clinical and Experimental Pathology 2014;(12):1333-1338
Purpose To investigate the expression of Beclin1, LC3 and mTOR in colorectal cancer ( CRC) and their significance. Methods Immunohistochemistry, Western blot and real-time PCR were employed to detect the expression of Beclin1, LC3 and mTOR in CRC. Results The positive expression rate of Beclin1, LC3 and mTOR in 242 cases of CRC was 90. 50%, 87. 19% and 46. 28%, respectively, which were higher than that in adjacent tissues ( P<0. 05 ) . Moreover, the expression of LC3 in moderately and poorly differentiated CRC was higher than that in well differentiated CRC, and the positive rate of LC3 in CRC with lymph node metastasis was higher than that in CRC without lymph node metastasis. The overexpression of mTOR was related to lymph node metasta-sis (P<0. 05), but both differentiation degree and lymph node metastasis were not associated with Beclin1 (P>0. 05). The expres-sion of LC3 was positively correlated with Beclin1 and negatively correlated with mTOR in colorectal cancer (rs =0. 593, P<0. 01, rs= -0. 165, P<0. 01), and the expression of Beclin1 was not associated with mTOR (P>0. 05). Kaplan-Meier survival analysis re-vealed that the five-year survival rate of patients without nodal metastasis, positive expression of Beclin1, LC3 and negative expression of mTOR was higher than those with nodal metastasis, negative expression of Beclin1 and LC3, and positive expression of mTOR. Cox survival analysis results revealed that LC3, mTOR and lymphnode metastasis were independent prognostic factors. The results of IHC, real-time PCR and Western blot in fresh CRC tissues indicated that the expression of Beclin1, LC3 and mTOR in colorectal cancer was significantly higher than that in adjacent tissues (P<0. 05). Conclusions The aberrant expression of Beclin1, LC3 and mTOR may be associated with the development and progression of colorectal cancer. The simultaneous detection of Beclin1, LC3 and mTOR genes in colorectal cancer may be helpful for the evaluation of the progressive degree and the judgment of prognosis.
6.Ultrasonic evaluation of superficial temporal artery-middle cerebral artery anastomosis in moyamoya disease
Lishu WANG ; Wen HE ; Jian SUN ; Dong ZHANG ; Dongying XIANG ; Hongxia ZHANG ; Huizhao LIU
Chinese Journal of Ultrasonography 2008;17(12):1030-1033
Objective To evaluate the value of ultrasound in assessing superficial temporal arterymiddle cerebral artery(STA-MCA)anastomosis in patients with moyamoya disease.Methods Ultrasonic Doppler studies were made pre-and postoperatively in 25 patients with moyamoya disease undergoing STAMCA anastomosis.The peak systolic velocity(PSV),end diastolic velocity(EDV),pulsatility index(PI)and resistance index(RI)were measured in external carotidartery(ECA) and superficial temporal artery (STA)pre-and postoperatively.The pattern of the collateral circulation through the bypass was categorized based on postoperative external carotid angiograms.Thus three categories were determined as extensive,moderate and poor.The patients were divided into three groups based on the pattern of the collateral circulation through the bypass,which was determined based on the angiographic findings.PSV,EDV,PI,RI of STA was compared between groups.Results ①The values of the PSV,EDV of anastomosed STA were significantly higher and the values of PI,RI were significantly lower after operation.②The values of the PSV,EDV of the anastomosed ECA tended to be higher and the values of PI,RI tended to be lower in postoperation patients,but the differences were not significant within preoperation and postoperation group.③The Values of the PSV,EDV of the anastomosed STA were significantly different among the groups.The PSV.EDV tended to be higher and the PI,RI tended to be lower in patients with more extensive bypass flow.④The blood flow direction of STA was from extracranial to intracranial.Conclusions The PSV,EDV,PI,RI of STA can predicting the extent of collateral flow through an STA-MCA bypass.Color Doppler ultrasonography is a noninvasive method of assessing the function of STA-MCA bypass in patients with moyamoya disease.
7.Application of ultrasound in assessing multiple burr-hole operations for moyamoya disease
Lishu WANG ; Wen HE ; Jian SUN ; Dong ZHANG ; Huizhao LIU ; Dongying XIANG ; Hongxia ZHANG
Chinese Journal of Ultrasonography 2009;18(8):698-701
more coincidence with ultrasound than SPECT. ConclusionsUltrasonography is a noninvasive method for assessing the effect of multiple burr-hole operation in patients with moyamoya disease.
8.Clinical analysis of Staphylococcus aureus resistance to methicillin in patients with coal worker's pneumoconiosis complicated by lung cancer.
Si-hai LIU ; Pei-yue LIU ; Wen FENG ; Jun-he DAI ; Cheng-dong QI ; Fang QIAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(5):391-392
9.Effect of yiqi huaju recipe combined with routine therapy in treating hypertension patients with metabolic syndrome: a clinical study.
Yi CHEN ; De-yu FU ; Xiao-dong FU ; Yan-ming HE ; Wen-jian WANG
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(6):680-687
OBJECTIVETo investigate the therapeutic effect of Yiqi Huaju Recipe (YHR) combined with routine therapy on the blood pressure, the blood pressure variability and other cardiovascular risk factors in hypertension patients complicated with metabolic syndrome (MetS).
METHODSTotally 43 hypertension patients complicated with MetS were recruited in this study and randomly assigned to the treatment group (22 cases, treated with basic routine treatment +YHR) and the control group (21 cases, treated with basic routine treatment + placebo). The treatment course was 12 weeks. Detected were parameters such as 24-h ambulatory blood pressure monitoring (ABPM), body mass index (BMI), waist circumference (WC), waist to hip ratio (WHR), homeostatic model assessment for insulin resistance (HOMA-IR), fasting glycosylated hemoglobin A1c (HbA1c), fasting plasma glucose (FPG), 2 h postprandial plasma glucose (2 h PPG), fasting plasma insulin (FPI), serum lipid, etc.
RESULTSThe anthropometric parameters and plasma glucose levels (except HbAlc) were obviously lowered after treatment than before treatment in the treatment group (P < 0.01, P < 0.05). Besides, better effects were obtained in the WC, WHR, 2 h PPG, FPI and HOMA-IR (P < 0.05). The average blood pressure amplitude, the blood pressure variability, and blood pressure load at any time point were more obviously improved in the two groups after treatment than before treatment (P < 0.01, P < 0.05). Besides, partial indices were better in the treatment group than in the control group (P < 0.01, P < 0.05).
CONCLUSIONSYHR combined with routine therapy exhibited better effect on reducing the blood pressure amplitude, the blood pressure variability, and the blood pressure load in hypertension patients complicated with MetS. It could also effectively decrease the risk of other vascular disease.
Adult ; Blood Pressure ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Hypertension ; complications ; drug therapy ; Male ; Metabolic Syndrome ; complications ; Middle Aged
10.Clinical applications of molecular biomarkers in urothelial carcinoma of bladder.
Liang CHENG ; Jia-wen XU ; Jia-jia HE ; Jing ZHAO ; Xiao-dong TENG
Chinese Journal of Pathology 2011;40(10):714-717
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Basigin
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analysis
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Biomarkers, Tumor
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analysis
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genetics
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Carcinoma, Transitional Cell
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drug therapy
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genetics
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metabolism
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Gene Expression Profiling
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Humans
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In Situ Hybridization, Fluorescence
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Inhibitor of Apoptosis Proteins
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analysis
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Mutation
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Neoplasm Recurrence, Local
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metabolism
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Nuclear Proteins
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analysis
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Receptor, Fibroblast Growth Factor, Type 3
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analysis
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genetics
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Tumor Suppressor Protein p53
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analysis
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genetics
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Urinary Bladder Neoplasms
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drug therapy
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genetics
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metabolism