AIM: To identify the changes in microRNA ( miRNA ) profile of human lens epithelium ( HLE) induced by H2 O2 and the role of miRNA in oxidative stress induced apoptosis.
METHODS: HLE cell line HLE - B3 was treated by 100μmol/L H2 O2 for 24h and the total RNA were isolated by Trizol reagent. miRNA profile was generated by miRCURYTM LNA microRNA Array. The target genes of differentially expressed miRNAs were predicted by bioinformatics software.
RESULTS:Twenty-eight miRNAs showed significantly differential expression after H2 O2 treatment, 18 miRNAs upregulated and 10 miRNAs downregulated. The differentially expressed miRNAs may involve in apoptosis of lens epithetium and development of cataract through targeting BCL2L2 and MIP.
CONCLUSION: H2 O2 can induce dramatically changes in miRNA profile of HLE, which may play a pivotal role in the pathogenesis and development of cataract.

Objective To study the chronology of development within puparia to determine the postmortem interval(PMI).Methods The necrophagous fly Lucilia cuprina was reared successively in constant temperatures(16℃,20℃,24℃,28℃,32℃).Sample,dissecte,and observe the pupal morphologic changes with the stereomicroscope.Results 7 morphological changes within the puparium can be used as age-markers and the pupal period of Lucilia cuprina can be divided into 9 developmental stages.The time required for each pupal stage was inversely proportional to the temperature.Conclusion Pupal developmental stages can be used as reference to the estimation of PMI.

AIM To study the flavonoids from Morus nigra L.leaves.METHODS The n-butanol fraction of 80％ ethanol extract from M.nigra leaves was isolated and purified by macroporous adsorption resin and preparative HPLC column,then the structures of obtained compounds were identified by spectral data.RESULTS Eleven compounds were isolated and identified as quercetin (1),icariside Ⅱ (2),rhodionin (3),rhodiosin (4),quercetin-3-O-β-(2-O-acetyl) galactopyranoside-7-O-α-arabia pyran glucosides (5),salidroside (6),kaempferol-3-O-β-(2-O-α-L-rhamnopyranosyl)-glucuronide (7),quercetin-3-O-α-rhamnopyranosyl-(1 →2)-β-glucopyranosiduronide (8),rhamnetin-3-O-galactoside (9),quercetin-3'-O-β-glucoside (10),quercetin-3-O-β-D-glucopyranoside (11).CONCLUSION Compounds 1-10 are isolated from this plant for the first time.

OBJECTIVE:To review economics of budesonide/formoterol and fluticasone/salmeterol for asthma systematically, and to provide evidence-based basis for rational selection of drug to treat asthma. METHODS:Retrieved from Medline,EMBase, EBM reviews (HTA,NHSEED),CBM,CJFD,Wanfang database,researches about pharmacoeconomics of budesonide/formoterol (test group) versus fluticasone/salmeterol (control group)for asthma were collected,and analyzed with qualitative systematic re-view method. RESULTS:A total of 7 researches and 13 comparative data were included,containing 6 682 patients. The incidence of severe acute asthma of test group were lower than those of control group,with significant difference(P<0.05). The total costs and direct costs of test group were lower than those of control group. Good effect had been achieved in test group with low cost. CONCLUSIONS:Budesonide/formoterol is more cost-effective than fluticasone/salmeterol in teenagers patients and adults who fail to control asthma. Due to limitation of included studies,large-scale and high-quality studies are required for further validation of the conclusion.

As a result of the increasing use of radiologic contrast medium in patients, especially the elderly in severe or clinical condition with attendant comorbidities, such as diabetes mellitus, renal failure, cardiac failure, and volume depletion. Contrast induced nephropathy(CIN)is now the third most common cause of hospital-aquired renal failure and accounts for approximately 11% of the cases. This article mainly introduces the evaluation of laboratory investigations providing insights into the pathophysiology of this disorder and the examination of the important clinical data of CIN including risk factors and diagnosis. The last section deals with renal protection and preventive strategies.

Objective To explore the impact of environment factors and social economic factors(environment interference factors for short)and control measures on the endemic status of schistosomiasis in Poyang Lake Eco?economic Region. Meth?ods The grey relational analysis model was applied to analyze the relationships between the key indexes of schistosomiasis epi?demic status and the environment interference factors and control measures in Jiujiang City of Poyang Lake Eco?economic Re?gion. Results Six environment factors,which included the annual average water level of Poyang Lake,average annual tempera?ture,storm frequency,annual average relative humidity,annual sunshine duration,and annual precipitation,had the most closed relationship with the indexes of schistosomiasis epidemic status(all ri>0.9). Among the socioeconomic factors,the num?ber of health technicians and beds of health facilities were most associated with the indexes of schistosomiasis epidemic status. Among the control measures of schistosomiasis,the number of cattle treated with extending chemotherapy,chemically killing of Oncomelania hupensis snails and eco?renovation were most associated with the indexes of schistosomiasis epidemic status. Con?clusion The various environment interference factors and their interaction should be considered in formulating the comprehen?sive control strategy for schistosomiasis,and the control strategy should be adjusted according to the epidemic dynamic and schistosomiasis?focused targets,so as to further strengthen the scientificity and validity of the control strategy.

Objective:To explore the postoperative complications of different incisions of parotidectomy in benign parotid tumor and the impact on life quality.Methods:62 patients with benign parotid ttmor underwent improved parotidectomy in our hospital from January 2010 to January 2015 were selected and randomly divided into group A and group B.The patients in group A were using improved S incision,and the patients in group B were using postauricular concealing incision.Then the perioperative indexes,complications after surgery and the influences to life quality of 2 groups were observed and compared.Results:The surgery time,blood loss,postoperative suction drainage and hospital stays of 2 groups had no great differences (P＞0.05).The early and forward complication rate of group A was 29.03 ％ and 25.81％ respectively,of group B was 19.35 ％ and 12.90 ％ respectively.There were no differences between them (P＞0.05).The scores of pain and emotion after surgery of group A were getting better,and appearance,smell and chewing function was getting worse than before surgery with statistically significance (P＜0.05).The scores of pain and emotion after surgery of group B were getting better than before (P＜0.05).The scores of appearance and emotion of group A were worse than those of group B with statistically significance (P＜0.05).Conclusions:Using postauricular concealing incision can obtain good life quality and safety for the patients with benign parotid tumor,which is superior to improved S incision,worthy of clinical applications.

Objective To further study the value of MR T2WI fat saturation sequence in acute Spinal Trauma. Methods 109 cases of acute Spinal Trauma underwent MR imaging were analysed retrospectively. The detection rate of lesions with bone marrow edema, Vertebral fracture and spinal cord injury was compared in T2WI and FS T2WI. Results FS T2WI could detect more lesions of obscure fracture and bone marrow edema than that in T2WI,and it could accurately show the extent and the feature of anatomic structure in acute injury of bone. Conclusion FS T2WI can improve the diagnostic sensition and accuracy in Spinal Trauma, and it should be used as a routine MR sequence for detecting Spinal Trauma.

Objective　To evaluate the effect of intravascular occlusion for carotid cavernous fistula (CCF) by analying the clinical features of CCF. Methods　Twelve cases with CCF were analysed on their medical histories, clinical features in the eyes, clinical examinations and the therapeutic effects of balloon occlusion in internal carotid artery.Results　Ten cases were treated successfully(83.33%), in which 2 cases were treated once more. There was no operation complication. The visual acuity of one case increased from no light perception to finger movement within 1 week preoperatively. The visual acuity of 10 cases improved 1～4 lines (Snellen chart). Some clinical symptoms and signs such as the lid, conjunctiva swelling, diplopia, the movement of eyeball and retinal hemorrhage were decreased remarkably. Carotid fistula of 12 cases with CCF were found to be occlusioned by digital subtraction angiography after operations.Conclusion　The early diagnosis and treatment for CCF are necessary. Color doppler flow imaging, CT and DSA are useful examination methods for diagnosis of CCF. Intravascular occlusion is the best therapeutic method for CCF nowadays.

Objective To evaluate regional and global left ventricular systolic function after coronary artery bypass grafting (CABG) by speckle tracking imaging (STI).Methods Transthoracic echocardiography (TTE) was performed in 32 patients with coronary heart disease (CHD) preoperative and 3 months after CABG.The two-dimensional data were obtained in apical 4-chamble,2-chamble and long axis view.Peak systolic longitudinal strain (SLs),global longitudinal systolic strain (GLS) and average global strain(GLS-Avg) were measured using STI.30 healthy volunteers were involved as controls.Results SLs in the control group increased from the basal to the apical ventricular.SLs in the control group and CHD group apical segment were significantly higher than the basal and middle segments.Compared to the control group,SLs,GLS in part of myocardial ischemic segments were lower in CHD group before CABG (P ＜0.05).SLs and GSL in the corresponding segments showed significantly improving 3 months after CABG in CHD group (P ＜ 0.05).There was good relationship between GSL-Avg and left ventricular ejection fraction.Conclusions STI technology can be used to objectively and quantitatively evaluate the change of regional and global left ventricular systolic function.Therefore,it provide a non-invasive means for evaluation of curative effect and prognosis judgement of CHD after CABG.