Objective To investigate the treatment effect of daily low dose of sildenafil treating interstitial cystitis .Methods 68 patients with interstitial cystitis in our hospital from June , 2012 to December, 2013 were divided into observation group and con-trol group by digital randomized method, 34 cases in each group.Observation group adopted daily dose of sildenafil 0.25 g treatment every day, and control group adopted the same dose of starch placebo every day.Two groups were treated for 3 months.Curative effect, micturition number a day before and after the treatment, the maximum bladder capacity, interstitial bladder inflammation shape index and problem index score ( ICSI, ICPI) , pelvic pain and urinary frequency score ( PUF) and quality of life scores ( QOL) of two groups were compared.Results The rate of significant curative effect and total effective rate of observation group were significantly higher than that of the control group, and the difference was statistically significant (p<0.01).Micturition number every day, the maximum bladder capacity, ICSI, ICPI, PUF and QOL scores of the two groups before treatment had no statistical significant differ-ences(p>0.05).Maximum bladder capacity and QOL scores of observation group after treatment were (265.44 ±43.85) ml and (44.15 ±4.69) respectively, which were higher than the (92.45 ±25.71) ml and (22.14 ±2.97) of the control group after treat-ment and (94.12 ±23.95)ml and (22.38 ±3.18) of observation group before treatment;Urine per day, ICSI, ICPI and PUF of ob-servation group after treatment is lower than that of the observation groups after treatment and that of the control group before treat-ment, and the difference had statistically significant (p<0.05).Conclusion Daily low dose of sildenafil for treating women with in-terstitial cystitis is worthy of clinical use for it can effectively relieve clinical symptoms and improve patient's life quality.

Objective To study the clinical features and diagnostic analysis of 30 cases with refractory myco-plasma pneumonia.Methods 60 patients with mycoplasma pneumonia treated in our hospital during 2012.03 to 2014.03 were selected, and 30 cases were diagnosed as normal mycoplasma pneumonia and the others as refractory mycoplasma pneumonia.The clinical manifestations, percentage of neutrophils, peripheral blood leukocytes , C -re-active protein (CRP) level, and chest radiographic were retrospectively analyzed , and the clinical judging indicators of refractory mycoplasma pneumonia were obtained by multivariate logistic regression analysis .Results Considering the time of fever, the difference between two groups was statistically significant (χ2 =12.27, p <0.01).The differ-ence of percentage of neutrophils , peripheral blood leukocytes and CRP between the two groups were all significant (p <0.05); Logistic regression analysis showed that time of fever >10 d, CRP >40 mg /L, appearance of large high -density lung opacities were the judging indicators of mycoplasma pneumonia refractory .Conclusion Time of fever, CRP level and imaging Findings are the judging indicators of mycoplasma pneumonia refractory .

The changes of the spinal cord blood flow(SCBF),the content of TXB2 and 6-keto-PGF1a of the injured segment of the cord and other neurological manifestations were observed on a rat model of spinal cord injury(SCI)established with Allen's weight drop(50g-cm)method and the effects of indomethacin on these changes were studied.It was found that SCBF was significantly reduced in the first 2 hours after SCI and further reduced in the 4th ~ 8th hour.Increase of TXB2 was observed in the 1st hour and reached the peak in the 4th hour.The level of 6-keto-PGF1a was also increased in the 1st hour and maintained at that level for 24 hours.The changes of TXB2/6-keto-PGF1a was similar to those of TXB2.There was a negative correlation of SCHF with TXB2 content and TXB2/6-keto-PGF1a ratio.The intravenous injection of 10mg/kg indomethacin could inhibit the increase of TXB2 content and increase 6-keto-PGF1a content relatively.It could also alleviate or retard the decrease of SCBF after SCI and improve the motar function of the hind limbs of the rats.These findings suggest that indomethacin can improve SCBF and promote the recovery of neurological functions through its regulatory effects on the levels of TXB2 and 6-keto-PGF1a

To explore the characteristics of hematological abnormalities in patients with systemic lupus erythematosus (SLE) through retrospective analysis.A total of 445 SLE patients were recruited and divided into 5 groups of low white blood cell,immune thrombopenic purpura,anemia,pancytopenia and control without hematological abnormalities.Among them,351 (78.9％) had hematological abnormalities and 94 (21.1％) without hematological abnormities.The prevalence of anemia,leucocytopenia,thrombocytopenia and pancytopenia were 56.0％ (246/445),27.6％ (123/445),13.5％ (60/445) and 8.5％ (38/445) respectively.The incidence of renal damage in anemia group was significantly higher than that in control group (P ＜ 0.01).SLE patients with hematological abnormalities had lower complement C3 levels (P ＜ 0.05) and higher systemic lupus erythematosus disease activity index (SLEDAI) (P ＜ 0.01).Anemia is the most common findings in SLE patients with hematological abnormalities who have lower complement C3 levels and higher SLEDAI.

This study was aimed to establish an identification method between leaflets of Dimocarpus longan and leaflets of Litchi chinensis. The leaflet morphological-venation pattern (LMVP) and quantitative analysis were reliable identification method for Chinese herbs. The results showed that the main differences of leaflets of Dimocarpus lon-gan were the eucamptodromous camptodromous pinnate venation; the secondary veins gradually changed into little near margin and a few brochidodromous; the type of tertiary vein was percurrent or reticulate. The main differences of leaflets of Litchi chinensis were brochidodromous camptodromous pinnate venation; the course of the secondary veins was sinuous or zigzag, and abruptly curved then linked; the type of tertiary vein was reticulate. With three groups of key differences mentioned above, both plants can be successfully identified from each other. The accuracy of identification results (AC) was from 98.1% to 100%. The agreement rate for observation (ARO) was from 98.5% to 100%. And the Kappa value was from 0.97 to 1.00. It was concluded that the established LMVP is simple, rapid, e-conomic and reliable in the identification between leaflets of Dimoc arp us longan and leaflets of its confused herb Litchi chinensis.

Objeetive To investigate the willingness to family care bed service and its influencing factors among the elderly with chronic diseases. Methods A three-stage random sampling method was adopted to carry out face-to-face questionnaire investigation in 652 community elderly patients with chron-ic diseases. Results The willingness to family care bed service was 57.2%.Its influencing factors includ-ed age,educational level,family economic income,activity of daily living,whether or not received treatment within 2 weeks and hospital stay within 1 year,whether or not understood the meaning of family care bed service or received it or similar at home service. Conclusions More than a half of the elderly with chronic diseases were willing to accept family care bed service.The community health service center or hos-pital should attaeh importance to and actively carry out family care bed service.They should enhance propanda work to induct the utilization of family care bed service by edlder people with chronic disease and tackle and satisfy their health requirement.

BACKGROUND: The expression of matrix metalloproteinases (MMPs) is enhanced in fibroblasts of aging skin; tripterine and ginsenoside Rd possess immunosuppression and anti-aging effects. Arotinoid ethyl ester can used to treat sun-induced aging skin, which accompanied by many side effects, OBJECTIVE: To discuss the regulation role of ginsenoside Rd, celastrol, arotinoid ethyl ester on MMP-1 and MMP-3 of the cultured fibroblasts of the human dermis in vitro. METHODS: The human dermis circumcision by surgical excision on newborn was obtained from Department of Surgery, Luzhou Medical College, and the informed consent obtained from patients. Culture of fibroblasts were as follow: ①No treatment in the normal control group. ②Radiated with 80 kJ/m~2 ultraviolet and treated by 100mg/L8-methoxypsoralen in the positive control group. ③Treated by ultraviolet radiation+8-methoxypsoralen+arotinoid ethyl ester in the arotinoid ethyl ester group. ④Treated by ultraviolet radiation +8-methoxypsoralen+triptolide in the triptolide group. ⑤Treated byultraviolet radiation+8-methoxypsoralen+ginsenoside Rd in the ginsenoside Rdgroup. The triptolide group was divided into 3 groups with doses of 10, 20, and 40 mg/L. The ginsenoside Rd group was divided into 3 groups with doses of 20, 50 and 100 mg/L. The immunohistochemistry staining of MMP-1 and MMP-3 of the fibroblasts in each group was observed. RESULTS and CONCLUSION: The expressions of MMP-1 and MMP-3 were significantly increased in the positive control group, which had a significant difference to the normal control group (P < 0.05). Compared to the positive control group, the expression of MMP-1 and MMP-3 significantly decreased in the treatment groups (P < 0.05); however, the differences among each treatment groups had no significance (P > 0.05). The results demonstrated that:①Ginsenoside Rd and tripterine exhibit comparably effect on prevent and cure sun-induced aging skin.②The wide ranges of effective concentration lead to little side effect to the body. ③The action mechanisms of ginsenoside Rd, celastrol and arotinoid ethyl ester in preventing and curing sun-induced aging skin is adjusting the expressions of MMP-1 and MMP-3.

Objective To observe the ultrastructure location of Huntingtin-associated protein 1(HAP1) in rat adrenal medulla and the effect of cold stress on the expression of HAP1 in rat adrenal medulla. Methods Fourteen healthy male Wistar rats were used in the present study and among them two rats were used for immune electron microscopy and twelve rats for cold stress experiment. In the cold stress experiment, animals were divided into control and cold groups randomly with six rats in each group. During the experiment, rats were housed in a room at the temperature of 4℃ for 12 hours and then immunohistochemistry and Western blotting were used to measure the expression of HAP1 in adrenal medulla. Results By using the immune electron microscopy, the results showed HAP1 was located on the external membrane of secretory vesicles of adrenal medulla and their membranous organelles. The expression of HAP1 in adrenal medulla of cold group significantly decreased as compared with that of control group ( P <0.01). Conclusion HAP1 might be related with the adrenal medulla cells endocrine granula and involved in the transmission and release of adrenaline or noradrenaline in secretory granule.

Objective The purpose of this study was to investigate the relationship of p185 and p53 protein overexpression with pathological types and recurrence.Antibodies against p185 and p53 proteins were used to measure expression of these proteins in the nuclei or cell membrane of cells from sections of the giant cell tumor of bone GCT.It showed that 11 out of 52 tumors overexpressed p185 protein and 14 out of 52 tumors had abnormally high levels of p53 protein,4 cases had abnormally high levels of both p185 and p53 proteins,positive expression rates of p185 and p53 in cases with recurrence and cases without reccarrence were 46 2%,20 5% and 38 5%,15 4% respectively.However,there was no association between p185 and p53-positive cases and pathological degree.There was significant correlation between the expression of p185 and p53 protein in the giant cell tumor of bone and recurrence.(? 2=6 125,P=0 047).However,there was no statitically significance between the expression of p185 and p53 protein in the giant cell tumor of bone and pathological types.So that,we consider that the clinical significance for p185,p53 overexpression in GCT to be researched further.

Objective To evaluate the biologic characteristics of a new type femoral stems either coated with gradient hydroxyapatite (HA) or additionally loaded with rhBMP-2. Methods Fifteen dogs were divided into 3 groups according to the implanted hip prosthesis. Each animal received hemiarthroplasty in the right hip with prosthesis made of pure Ti-6Al-4V femoral stem (Ti group), the new gradient HA-coated femoral stem (HA group) with 100 to 150 ?m hydroxyapatite coating, and the new gradient HA-coated femoral stem loaded with rhBMP-2 of 9 mg (BMP-HA group) respectively. The dogs were sacrificed at 12 weeks after operation, and labeled with tetracycline 3 of 30 mg/kg days prior to sacrifice, and then the histological changes of the tissue surrounding the prosthesis were evaluated. The radiographic examinations were taken before and after sacrifice. Results On radiographic examination, local radiolucent line around the prosthesis was observed only in one specimen in Ti group. The new bone formation was found both in HA group and BMP-HA group more obviously than in Ti group. Under microscopy, it was noted that this new gradient HA-coating was intact and no obvious biodegradation in most section, whereas a fibrous membrane was found in Ti group. The average rates of interface osteointergration in Ti group, HA group and BMP-HA group were 4.05%?7.66%, 71.04%?9.81% and 88.86%?6.56%, respectively, those of HA and BMP-HA group were significantly higher than that of Ti group (P