Child ; Humans ; Hypertension, Pulmonary ; drug therapy ; Phosphodiesterase Inhibitors ; therapeutic use ; Piperazines ; administration & dosage ; therapeutic use ; Purines ; administration & dosage ; therapeutic use ; Sildenafil Citrate ; Sulfones ; administration & dosage ; therapeutic use

ObjectiveTo explore the nursing students'puzzles about career planning,and provide a basis for the training.MethodsA qualitative research was conducted on 14 university nursing interns by in-depth interview and observing.The whole interview was noted.The data was analyzed according to Colaizzi's phenomenological procedure.ResultsThe experience could be classified into four aspects,including hazy understanding of career planning,lack of understanding of themselves,limited ca reer identity and lack of social cognition.ConclusionNursing educators should enhance the training against the nursing students'puzzles and make sure they can make a good career planning.

Objective:To explore the combined treatment rigimen of gonococcal prostatitis.　Methods:The combined treament of improving remedy of antibiotics was used by taking orally the Chinese medicine and herb medicine demibain in 54 cases of patient with gonococcal prostatitis. compared with controlled group.　Results:The curing rate of combined treatment group was 74.1%, being higher significantly than that of controlled group (P＜0.05).　Conclusion:The combined treatment is better than the controlled group in curing gonococcal prostatitis and preventing recurrence.

Objective To assess the effect of swallowing function training on dysphagia.Methods66 patientswith dysphagia were randomly divided into training group and control group. The swallowing function of the two groupswere evaluated before and after training.ResultsThe effective rate of training group is 78.1% which is higherthan control group (P< 0.01).ConclusionSwallowing function training can significantly improve swallowing function and activity of daily living of the patients with dysphagia.

Objective To observe the effects of different gastrointestinal reconstruction methods after dis -tal subtotal gastrectomy on nutritional condition and blood glucose in patients with type 2 diabetes ( T2DM) and gastric cancer.Methods 88 patients with T2DM and gastric cancer undergoing radial distal gastrectomy were studied and among them 43 patients had Billroth I gastrointestinal reconstruction , 22 patients had Billroth II gas-trointestinal reconstruction and 23 patients had Roux-en-Y gastrointestinal reconstruction .Body mass index ( BMI) , serum albumin and prealbumin of the 3 groups were measured preoperatively and 1 year after surgery . The patients were followed up .Results There was no significant difference among the 3 groups in preoperative values(P>0.05).One year after surgery, BMI, serum albumin and peralbumin in the 3 groups had different de-grees of reduction , but showed no statistical difference ( P>0.05 ) .The effective rate of diabetes control was 18.60%in Billroth I group , 72.73%in Billroth II group , and 73.91% in Roux-en-Y group and the difference had statistical significance(χ2 =17.390 3,P<0.05).The effective rates of diabetes control in Billroth II group and Roux-en-Y group were higher than that in Billroth I group (χ2 =18.340 9,P<0.05;χ2 =19.480 4,P<0.05), and there was no evident difference between Billroth II group and Roux-en-Y group(χ2 =0.008 1,P=0.928 4).Conclusion Billroth II and Roux-en-Y gastrointestinal reconstruction can improve glycemic metabo-lism of patients with T2DM and gastric cancer without significantly reducing the nutrition status .

Objective Establishment and characterization of healthy donor's ??T cell clones.Methods ??T cells were cloned by limiting dilution after positive sorting with 60Co irradiated allogeneic PBMC as feeder cells.Flow cytometry analysis and molecular biology technique were then used to identify ??T cell clones.MTT assay was used to verify their proliferation after incubated with epitope peptides recognized by ??T cells.Results A ??T cell clone had been established.The subtype of this clone was V ?9 V ?2 without expression of CD4 and CD8.Further studies indicated that epitope peptide EP6 could not only specifically bind to ??T cell clone but also trigger the proliferation of ??T cell clone.Conclusion A healthy donor's ??T cell clone was successfully established,which laid a solid foundation for further study on ??T cells.

The expression patterns of 5-hydroxytryptamine(5-HT),gastrin(Gas),β-endorphin(End),somatostatin(SS),vasoactive intestinal peptide(VIP),glucagon(Glu) and neuropeptide Y(NPY)-positive cells in the duck thymus were studied by the immunohistochemical method associated with image analysis.The result shows that these 7 kinds of neuroendocrine cells with strong intensity were differentially detected in the duck thymus.The End-positive cells were abundantly expressed in the thymic cortex,whereas the Gas,5-HT,SS,VIP,Glu and NPY ones were more frequent in the medullary and around the corticomedullary junction.Except Glu,the other 6 kinds of neuroendocrine substances with various intensity were expressed in the cells of thymic corpuscles.The observation demonstrated that the duck thymus is not only a primary lymphatic organ,but also has important neuroendocrine functions.The End-positive cells in the thymus may play crucial roles in the development of immature T-lymphocytes.The neuroendocrine cells with a predominant distribution in the medulla and around the corticomedullary junction might facilitate exerting their influence via an endocrine,autocrine or paracrine pathways on the function of mature T-cells.The possible roles of thymic corpuscles are also discussed.

Objective To investigate the diagnosis and treatment of injury in choledocho-pancreatico-duodenal junction. Methods The clinical data of 6 patients with injury in choledocho-pancreatico-duodenal junction who had been admitted to Beijing Hospital from January 2000 to January 2008 were retrospectively analyzed. Results Of the 6 patients, 4 were diagnosed according to the intraoperative findings, cholangiography and fiber cholangioscopy. The 4 patients were cured after suture of the perforation in the choledocho-pancreatico-duodenal junction, T-tube drainage and abdominal drainage. Two patients developed severe abdominal and retroperitoneal infection and other complications after operation, and were diagnosed by cholangiography and fiber cholangioscopy. Of the 2 patients, 1 was cured and 1 died after multiple drainage procedures and debridement. Conclusions Diagnosis and treatment in the early stage are crucial for the curative purpose. Cholangingraphy and fiber cholangioscopy are effective in the diagnosis of injury in choledocho-pancreatico-duodenal junction. The suture of the perforation in the choledocho-pancreatico-duodenal junction, T-tube drainage and abdominal drainage should be chosen for patients who are diagnosed during primary operation. For patients with abdominal and retroperitoneal abscess and cellulitis, drainage and debridement should be performed, and biliopancreatic diversion and duodenal diverticularizatian are applied to patients when necessary.

Calcineurin inhibitors,such as cyclosporin A and tacroli mus,are widely-used i mmuno-suppressive drugs clinically.However,renal toxicity,hyperglycae mia and other side-effects can occur over long-term use.Isolation of novel calcineurin inhibitors fro m medicinal plants in recent years has pro-vided a new approach to the development of new immunosuppressive drugs with high efficiency and low toxicity.Recnt studies have shown that medicinal plant extracts, such as isogarcinol, quercetin, kae mpferol,phenylethanoid glycosides,ere mophilane sesquiterpenes A and B,pisiferdiol,as well as extracts fro m Smilax china L and Jasminum humile L,have obvious inhibitory effect on calcineurin.

Combined pulsed-field gradient, shaped pulses, and product operator calculations (algorithm), two-dimensional 1 H-31 P hetero-nuclear multiple quantum coherence ( HMQC) pulse sequence was established with the aid of C language. The result showed that coherent transfer path selection could be achieved by adjusting the ratio of two gradient strength ( g1 , g2 ) . The best ratio of g1 and g2 was -5:4 . The HMQC method based on the above sequence can be used for identifying the alkyl groups coupling with phosphorous nucleus. For the different alkyl groups, the sensitivity of this method decreased in the order methyl>isopropyl>ethyl>propyl. The limit of detection was about 10 mg/L. This method can be directly used for the screening and identification of phosphorus chemical warfare agents and related compounds.