Objective To study the effects of protein kinase C (PKC) inhibitor staurosporine (STS) on the proliferation and apoptosis of human cholangiocarcinoma QBC-939 cells and to explore its possible mechanism. Methods CCK-8 was used to detect the effects of PKC inhibitor STS on the proliferation of human cholangiocarcinoma QBC-939 cells. The effects of STS on the ultrastructural characteristics of QBC-939 cells were observed by routine transmission electron microscopy (TEM). The apoptosis rate and the cell cycle distribution of QBC-939 cells were detected by flow cytometry. The expression of cyclin B1,Cdk1 and p-Cdk1 in QBC-939 cells was detected by Western blotting. Results STS could significantly inhibit the proliferation of QBC-939 cells in a dose-dependent manner (P <0. 05) and the half inhibitory concentration ( IC50 ) of QBC-939 cells at 24th and 48th h was 334 nmol·L-1 and 118 nmol · L-1 , respectively. TEM observed that STS could induce typical apoptotic bodies and super-microstructural changes of QBC-939 cells. By Annexin V-FITC/ PI double labeling flow cytometry,we found that the apoptotic rate of QBC-939 cells after treatment with STS for 0,12,24 and 48 h was (10. 16±4. 52)% ,(22. 35±2. 19)% ,(34. 27±2. 30)% and (59. 70±5. 97)% ,respectively. By flow cytometry,compared with the control group,STS could significantly increase apoptosis rate of QBC-939 cells,decrease the percentage of cells in G0 / G1 phase and increase the percentage of cells in G2 / M phase (P<0. 05). Western blotting proved that the expression levels of cyclin B1 and Cdk1 proteins in the STS-treated QBC-939 cells were significantly decreased (P<0. 05),while the expression level of p-Cdk1 protein in the STS-treated QBC-939 cells was significantly increased ( P < 0. 05 ). Conclusion STS can significantly inhibit cell proliferation and induce apoptosis of human cholangiocarcinoma QBC-939 cells and the mechanism may be related to cell cycle arrest at G2 / M phase.

This review article briefly described the interaction and inner link between chemotherapy tolerance and metastasis of breast cancer.The common pathways of occurrence of drug resistance and metastasis were analyzed with formation and outcome of breast cancer epithelial-mesenchymal transition (EMT) and with enrichment and function of breast cancer stem cells.The chemotherapy which play positive role in clinical practice was pointed out that may cause negative effects by changing tumor microenvironment.Those evidences show that the theory on tumor microenvironment will help to formulate the research strategies for preventing and reversing resistance of breast cancer to drug and metastasis.

In view of the imbalance between basic research and clinical research in cancer,and the failure of timely and effective translation from basic achievement to clinical application,new insights to strengthen and accelerate the bidirectional translation between clinical issues and basic achievement have being widely accepted,with the definition of Translational Medicine as a link.With the rapid development of modern molecular biology and cell biology techniques,the strong power of high-throughput deep sequencing,epigenetics,and microenvironment research on exploring cancer biomarkers and interpreting the mechanisms of carcinogenesis and progression,has being taken seriously.Here,we will illuminate the role and further application of deep sequencing,epigenetics,and microenvironment based on analysis from clinical issues or samples in the current cancer research.

Objective To evaluate the selenium nutrition level of Huaibei inhabitants and to supply valid data to Chinese Microelement Nutrition Database. Methods 513 healthy people living in Huaibei for more than 10 years were selected randomly from September 2003 to June 2004. The selenium content in hair was measured by catalytic polarographic method to delimit selenium richness region. According to the selenium content in hair,the intake of selenium from food was estimated. The investigation results were evaluated by the standard of Chinese Dietary Reference Intakes. Results The selenium content in hair was (0.390 3?0.104 5) ?g/g in Huaibei inhabitants,only 51.9% of the people reached the proper level. The average intake of selenium of Huaibei inhabitants was (31.92?9.93) ?g/d,which showed that the selenium nutrition level of Huaibei inhabitants was lower than the standard of Chinese Dietary Reference Intakes except children between 0 to 6 years old. The percent which satisfied RNI and EAR standard occupied 19.9% (102/513) and 32.9% (169/513) respectively,which were also much lower than the 50% of physiological necessary level. Conclusion The selenium intake from food in Huaibei inhabitants was scarce obviously. To increase the selenium intake properly will be a wise selection.

Morphine antagonist naloxone (3mg/kgiv ) induced a rise in blood pressure,pulse pressure & respiratory rate in the rat under hemorrhagic shock. It also improved the survival rate. ?Receptor antagonist pheno-xybenzamine ( 1 mg/kg iv ) or adrenalectomy abolished the beneficial effects of naloxone. Reserpine ( 1 mg/kg ip for 5 d) which depleted catecholamines of periphal sympathetic nervous system could not abolish naloxone effects. Resrpine plus adrenalectomy abolished naloxone actions again. It is suggested that the effect of naloxone on the blood pressure is mainly due to release of catecholamine from adrenalmedu-llary. The results of isolated adrenal perfusion indicated that naloxone could not induce a rise in catecholamine concentration of perfused liquid. It is suggested that the action of naloxone on catecholamine release may not be a result of direct action upontbe chromaffin cells.

In order to further the progress of the quality of practice teaching in medical imageology,different teaching methods in practice teaching should be taken into account such as the features of teaching,knowledge structure and learning ability in medi- cal imageology.

Objective To explore the effect of traditional Chinese medicine direction inductive therapeutical instrument combined with golden bifid in the treatment of infantile diarrhea .Methods 188 infants aged from 1 month to 3 years who suffered from acute diarrhea were randomly divided into the treatment group and the control group ,94 cases in each group .All the patients were treated with continued diet ,prevention and correct water and electrolyte bal-ance and other treatment ,the treatment group received traditional Chinese medicine direction inductive therapeutical instrument combined with oral golden bifid based on the above treatment .The time of temperature returned to normal , stop vomiting and diarrhea and stool frequency were recorded and analyzed .Results The time of symptoms disap-peared,obvious effective rate and total effective rate of the treatment group were better than those of the control group . Conclusion It is safe and effective to treat infantile diarrhea by traditional Chinese medicine direction inductive therapeutical instrument combined with golden bifid .

Computer science is not important enough in the medical university curriculum and students do not like this course. Therefore, game-teaching methods were employed to enrich teaching methods,to make medical teaching more vivid and to promote medical students' learning interests. In this paper,application of game-learning idea in the medical teaching process was discussed from multiple aspects including the story of the game,incentives of the game and the punitive measures of the game.

Cone-beam computed tomography(CBCT) was used to analyze the anatomic morphology of maxillary canine fossa of different sagittal skeletal patterns patients;implants of various dimensions were virtually placed into the maxillary premolars region.The relationships among maxillary canine fossa and implant were analyzed.The depth of the maxillary canine fossa was(4.33±0.73),(3.77±0.58),(5.18±0.93)mm in the 1st premolar region of class Ⅰ,Ⅱ and Ⅲ respectively.The depth of the maxillary canine fossa was(3.20±0.63),(2.81±0.58),(3.90±0.79)mm in the 2nd premolar region of class Ⅰ,Ⅱ and Ⅲ respectively.There were no significant statistical differences in genders,sides,and loss of tooth.And there were significant statistical differences in three classes(P<0.05).The length of the implants was (10.30±1.70)mm(d=3.3 mm) and (8.77±1.58)mm(d=4.1 mm) in the 1st premolar region and (8.09±1.51)mm(d=3.3 mm),(6.69±1.35)mm(d=4.1 mm) in the 2nd premolar region in patients with teeth and buccal perforation;the length of the implants was (8.98±1.54)mm(d=3.3 mm) and (7.67±1.52)mm(d=4.1 mm) in the 1st premolar region and(7.09±1.59)mm(d=3.3 mm) and (5.79±1.34)mm(d=4.1 mm) in the 2nd premolar region in patients with tooth loss,respectively.There were significant statistical differences in loss of tooth(P<0.05).Analyzing of spatial relationships among maxillary canine fossa and implants of different sagittal skeletal patterns patients especially class Ⅲ patients,by using CBCT has important significance in guiding dental implants.

EBP50(ERM-binding phosphoprotein-50),a multifunctional adapter protein with 358 amino acids and two PDZ domains, regulates cell growth and migration. Lines of evidences indicate that it is a potential cancer suppressor protein. Loss of heterozygosity (LOH) and intragenic mutation of the ebp50 gene have been found in both primary breast tumors and breast cancer cell lines. EBP50 suppresses the breast cancer cell proliferation via its interaction with many tumor suppressor protein including PTEN, SYK, MERLIN, etc. Here the molecular structure of EBP50, signal pathway regulated by EBP50, and the relationship between breast cancer development and EBP50 are discussed.