On the basis of the bioluminescent detection technology of the lux gene being a kind of new and progressing environmental detection technology,in this paper,the constitution,function,expression and regulation of the lux gene were introduced,moreover,the recent advance of its application in the study of environmental detection was reviewed

The coming of an aged society in China will surely greatly affect the chief concerns of health care. The Chinese orthopaedists should be prepared for the tasks imposed by the new situation. Once a fracture happens due to osteoporosis in a senile person, a series of problems will follow. This paper discusses the diagnosis and prevention of osteoporosis, preoperative assessment of the patient, proper application of internal fixation and combined treatment of fracture and osteoporosis. The critical point is how to improve the therapeutic effects and to reduce the operative risks.

This paper firstly discusses the concept and manifestation of subhealth state,and reveals that the subhealth state is a low constitutional one between morbid state and health state.The manifestations of subhealth state include two cases,namely symptoms without positive lab or instrument examination results and tiny positive lab or instrument examination results without symptoms.In the diagnosis of subhealth state,traditional Chinese medicine comes down the traditional four diagnostic methods of which tongue-inspection,pulse-taking and complexion-inspection are particularly significant,and mainly makes use of etiological syndrome differentiation and differentiation of syndromes by zang-fu viscera.Traditional Chinese medicine has multifarious and effective therapeutic methods in the treatment of subhealth state,including Chinese herbs as well as acupuncture,massage,leading of Qi,food treatment and Qigong.Adjustment of emotion,regard of living,moderate work and rest,reasonable diet are important in the auxiliary treatment of subhealth state.In the new era,we should actively utilize the advantages of traditional Chinese medicine in the diagnosis and therapy of subhealth so as to contribute more to the health of people.

29 virgin female Wistar rats were divided into experimental and control groupson the 5th day of pseudopregnancy.The experimental animals were injected with200 ?g.of LH-RH-A subcutaneously daily in 5th-8th day,while in the controlgroup physiological saline was used instead.Both experimental and control animalswere killed on 9th,11th,13th days of pseudopregnancy.Histochemical techniques were applied to cryostat sections to show the activityof the following enzymes:SDH,LDH,NE,~5△A-3?-HSD,G6Pase,TPPase,AlPand AcP;PAS,Oil red O,and gallocyanin-chromalum staining methods were usedto show carbohydrates,fats and RNA respectively.The above enzymatic reactions of the decidual cells of the experimental animalswere negative,while those of the stromal cells were positive on 9th day.Incomparison,the results of the decidua of the control group on 13th day are verysimilar to the decidua of the experimental group on the 9th day.The resultssuggest that a high dosage of LH-RH-A only advances degeneration of thedecidua.

Transdermal drug delivery is an administration route which can avoid the first-pass effect,maintain steady plasma concentrations and enhance bioavailability. Drug transporting through the skin by passage through the stratum corneum leads to the viable epidermis and the dermis.With the development of the computer technology,many mathematical models for predic-ting the absorption of drugs have been built according to physical and chemical properties of drugs and physiological characteristics of each skin layer.This article presented provides a summary of the progress of mathematical models for predicting percutaneous absorption of drugs.

Objective To investigate the effect of ATP-binding cassette protein E 1 (ABCE1) gene silencing by electroporation on the survival,cell cycle and invasion of human glioma cells line U87MG.Methods The siRNA against ABCE1 was constructed and transfected into U87MG cells by electroporation.The expression of ABCE1 was detected by real time-polymerase chain reaction (RT-PCR) and Western blot.Flow cytometry was used to detect the cell cycle distribution and apoptosis.The effects of ABCE1 gene silencing by electroporation on proliferation,migration and invasion of U87MG cell line were evaluated by cell counting kit-8 (CCK-8) assay,wound closure assay,chemotactic migration,and cell invasive experiments,respectively.Results Compared to the control and blank groups,the mRNA and protein levels were significantly decreased in the experimental group when ABCE1 gene silencing by electroporation.The cell cycle was arrested at G0/G1 phase,and cell number in S phase was decreased in U87MG cell line (P ＜ 0.05).The cell growth inhibition ratio in the experimental group was significantly higher than that in the control and blank groups (P ＜0.01).Compared to the control and blank groups,the experimental group U87MG cell proliferation was inhibited significantly (P ＜ 0.05).Scratch healing experiments showed the experimental group migration ability was decreased significantly (P ＜ 0.05).Transwell chamber method showed the experimental group U87MG cell invasion ability was decreased significantly (P ＜ 0.05).Conclusions ABCE1 is involved in the progression of human glioma cells,and inhibiting the expression of ABCE1 by electroporation can decrease migration,invasion,and proliferation ability of tumor cells in vitro.

Objective To assess the predictive value of cerebral arteriosclerosis to acute cerebral infarction evaluated by transcranial doppler ultrasound .Methods 170 cases suspected cerebral arteriosclerosis consecutively enrolled were given transcranial doppler ultrasound and were followed up for 6 months,then we assessed the correla-tion between the levels of cerebral arteriosclerosis and acute cerebral infarction within 6 months.Results By Spearman rank correlation analysis ,there was positive correlation between the severity of cerebral arteriosclerosis de-tected by transcranial doppler ultrasound and acute cerebral infarction within 6 months,the correlation coefficient was 0.575(P<0.05).Conclusion Through the detection of transcranial doppler ultrasound ,the higher levels of cere-bral arteriosclerosis,the greater probability of acute cerebral infarction within 6 months.

BACKGROUND:Currently the ultrasound-guided continuous femoral nerve block has been widely used as a postoperative analgesia method after knee replacement surgery, but there are stil some technical and practical details issues to be studied. OBJECTIVE:To evaluate the effect of ultrasound-guided imaging and safety evaluation of different puncture techniques and needle choice in the ultrasound-guided continuous femoral nerve block after total knee arthroplasty.METHODS:One hundred and sixty patients undergoing total knee arthroplasty were randomly divided into four groups, with 40 cases in each group. Continuous femoral nerve block was done after general anesthesia induction. Patients in each group were respectively punctured using 20 G needle (diameter 1.1 mm) out-of-plane approach, 20 G needle (diameter 1.1 mm) in-plane approach, 18 G needle (diameter 1.3 mm) out-of-plane approach, and 18 G needle (diameter 1.3 mm) in-plane approach. Patient-control ed analgesia pump was used 30 minutes before the end of surgery. The operating time of continuous femoral nerve block was recorded. The visual analogue scale scores at rest, active functional exercise and continuous passive movement state on 6, 24, 48, 72 hours after total knee arthroplasty were estimated. The presses of the pump, time of first walk, daily walk times and complication of continuous femoral nerve block were observed. RESULTS AND CONCLUSION:The operating time of continuous femoral nerve block in group D was shorter than that in other groups (P<0.05). There was no difference of visual analogue scale scores, the presses of the pump, time of first walk, and daily walk times among each group. The incidence of puncture site pain in group C was higher than that in other groups (P<0.05). The best ultrasound imaging was obtained by using 18 G needle in-plane approach for ultrasound-guided continuous femoral nerve block after total knee arthroplasty, and the complication incidence was not increased.

Objective To investigate the protective effect of resveratrol ( RES) against hydrogen peroxide ( H2 O2 )-induced oxidative injury to astrocytes and the related mechanism. Methods Subcultured astrocytes were randomly divided into four groups:negative control group ( treated with normal culture medium) , model control group ( treated with 100 μmol·L-1 H2 O2 for 12 h), resveratrol low dose group (treated with 20 μmol·L-1 RES for 24 h H2O2 for 12 h) and resveratrol high dose group ( treated with 40 μmol·L-1 RES for 24 h before H2 O2 for 12 h) . Cell viability was detected by MTT assay, apoptosis rate was detected by flow cytometry, apoptotic cell morphology was detected by hochest33258 staining, and the expression of apoptosis-related factors such as caspses-3 and caspase-9 were measured by colorimetric detection. Results MTT assay showed that after treatment with 5, 10, 20, and 40 μmol·L-1 RES for 24 h, cell viability was (100. 46±3. 17)%, (101. 33± 3.14)%, (101. 33±1. 30)%, and (99. 67±2. 62)%, respectively, and the difference was not statistically significant as compared with the negative control group [(98. 33±2. 13)%, P>0. 05]. RES showed no effect on astrocyte activity, after treatment with 20 and 40 μmol·L-1 RES, astrocyte activity was significantly elevated to (54. 67±4. 11)% and (70.33± 2. 61)% as compared with model control group (t=3. 59, 7. 13, P<0. 05), RES inhibited hydrogen peroxide-induced decrease in cell viability. Flow cytometry results showed that after treatment with 20, 40 μmol·L-1 RES, the apoptosis rate of astrocytes significantly decreased to (35.51±3. 56)% and (14. 12%±3. 19)% (t=4. 26, 6. 33, P<0. 01) as compared with model control group (46. 31±4. 16)%. Hochest 33258 staining showed that RES inhibited hydrogen peroxide-induced cell apoptosis, besides, the RES treatment also could reduce H2 O2-induced expression of caspses-3 and caspase-9 in astrocytesin a time-dependent manner. Conclusion RES can inhibit hydrogen peroxide-induced astrocytes apoptosis through inhibiting the expression of caspses-3 and caspase-9, which can provide experimental evidence for its treatment of central nervous disorders.

Objective:To evaluate color doppler ultrasound diagnosis value of cesarean scar pregnancy.Methods: Using color doppler ultrasound probe belly and vaginal probe, faceted searches in patients with suspected scar pregnancy, observe suspicious gestational sac or clutter echo of the location, size, shape, and presence of complications, and radiography, video records, follow-up.Results: The abdominal and vaginal color to exceed cesarean scar pregnancy can make a definite diagnosis.Conclusion: Color doppler flow imaging (CDFI) in cesarean scar pregnancy has a characteristic ultrasonographic performance, ultrasound can accurately make a clear diagnosis. It is the first choice for clinical diagnosis.