Objecitve To observe the relationship between the expressions of the estrogen receptor (ER),progestogen receptor (PR) and tumor infiltration and metastasis in thyroid cancer.Methods By using immunohistochemical method to study the relationship between the expressions of ER and PR in 100 cases of thyroid carcinamas and 28 cases of benign thyroid lesions.Results The positive rates of ER and RP expression were 67 0% and 62 0% respectively in thyroid carcinomas,it had correlation with well differentiation and type of histology but positive expression did not relate to age and sex.The positive rates of ER and PR in the non-metastasized group were 75 4% and 70 5% respectively;significantly higher than that of the metastasized group which were 53 8% and 48 7% respectively (P

Aminoacyl-tRNA synthetase catalyzing the first reaction of protein biosynthesis. In mammalian cells, eight aminoacyl-tRNA synthetases (aaRSs) and three auxiliary protein factors form a macromolecular aminoacyl-tRNA synthetases complex (aaRS complex). The three nonsynthetase protein factors, namely, p43, p38, and p18 were found to be involved in many other important life activities besides their roles in the complex. The auxiliary factor p43 was the precursor of endothelial monocyte activating polypeptideⅡ (EMAPⅡ), which involved in angiogenesis and apoptosis. The auxiliary factor p38 was crucial for the development of lung, and its abnormal accumulation in neuron would be related to the Parkinson’s disease. The auxiliary factor p38 and p18 could promote the repair of DNA damage via different pathways in a highly organized way. All these breakthroughs enhance our understanding about the interaction between the aaRS complex and the macromolecular signaling network and promote the studies on this field.

Objective To evaluate the quality of examination papers of otolaryngology among students of grade 2007 in the first clinical college of Chongqing Medical University.Methods Totally 202 copies of examination papers of otolaryngology from grade 2007 students in our college were taken as research references.SASS 15.0 software was used to statistically analyze total score of every student and score of each question.Mean score,standard deviation,difficulty coefficient and discrimination coefficient of each question were counted and normality test was conducted.Results Scores were in negative skewness distribution,with mean score of 80.9,standard deviation of 10.9,the lowest score of 56.5,the highest score of 98.0,difficulty coefficient of 0.781,and discrimination coefficient of 0.308.Conclusions Examination papers are well designed and discriminated and can objectively reflect students' command of the course.However,distribution of sores is unreasonable and examination paper is relatively easy to complete,indicating the weakness in the teaching process.

Objective To analyze the inducement,clinical manifestations,diagnosis and treatment of diabetes patients with multiple organ failure(MOF).Methods A retrospective analysis of 64 diabetes patients with multiple organ failure was executed,the clinical data were statistically analyzed.Results Heart failure was the most common in 64 cases with a incidence of 100.0％,the rest common failure organ were kidney(92.2％ ),brain( 32.8％ ),lung ( 14.1％ ),gastrointestinal tract (3.1％ ),and liver( 1.6％ ).Cases with the glucose level over 33.5mmol/L illustrated a significantly higher incidence than other groups ( P ＜ 0.05 ),glucose level between 22.5 and 33.4mmol/L group was significantly higher than the 12.2 ～ 22.4mmol/L group( P ＜ 0.05 ) The inducement of MOF were infection (76.6％ ) medication terminate ( 9.4％ ),ketoacidosis ( 7.8％ ),cardiovascular and cerebrovascular accidents (6.3％ ).Conclusion Positive control of blood glucose in the early stage of diabetes,prevent complications,eliminate the incentive could reduce the incidence of MOF,eventually reduce the mortality rate.

Objective To explore the clinical application value of combined detection of serum brain natriuretic peptide (BNP),homocysteine(HCY) and blood lipids in the patients with heart failure.Methods The levels of serum BNP,HCY,TC,TG,HDL-C,LDL-C,ApoA,ApoB and Lpa were detected in 100 patients with heart failure (observation group) and contemporaneous 100 persons (control group) undergoing healthy physical examination.Then the comparative analysis was performed.Results Compared with the control group,the BNP and HCY levels in the observation group were significantly increased(P＜0.05);among 7 indicators of the blood lipid,the Lpa level in the observation group was increased compared with the control group,while the TC,HDL-C and LDL-C levels were significantly decreased(P＜0.05),and the TG,ApoA and ApoB levels had no statistical differences between the two groups(P＞0.05).Conclusion Serum BNP,HCY,TC,HDL-C,LDL-C and Lpa levels have close correlation with heart failure,especially the combined detection of BNP,HCY and Lpa,which has predictive and diagnostic value in heart failure and is worthy of clinical popularization and application.

Objective To evaluate the value of the machine-drawn time-intensity curves with intravenous contrast-enhanced color Doppler imaging in study of perfusion of normal kidneys of the rabbits.Methods A series of sequent pictures of 16 normal kidneys of 8 rabbits with color Doppler flow imaging(CDFI) and color Doppler power imaging(CDPI) were collected,and time-intensity curves were drawn by the ultrasound instrument after intravenous administration of "Quanfuxian" contrast medium.Quantitative analyses were done by moving the "interest line".Results The time-intensity curves showed as single apex with steep ascending and slowly descending branch after contrast enhancement.Conclusions The machine-drawn time-intensity curves with intravenous contrast-enhanced color Doppler imaging could efficiently show the perfusion of kidney.

To investigate the changs in the expressions of cellular adhesion molecules (CAMs) of the myocardium after low dose interleukin 1? (IL 1?) pretreatment and the relationship between CAMs and delayed cardioprotection, we measured the change in the expressions of intercellular adhesion molecule 1 (ICAM 1) mRNA and its proteins, the expressions of leukocyte function antigen 1(CD11a) proteins, and infiltration number of polymorphonuclear leukocytes (PMNs) in the myocardium and determined the infarct size with in situ hybridization(ISH),immunohistochemistry and enzyme methods immediately,12 hours and 24 hours after IL 1? pretreatment in a model of ischemia/reperfusion of myocardium in rats. The results showed that the expressions of ICAM 1 mRNA and its protein, CD11a protein and the PMN infiltration number were significantly higher in the ILPC and NS groups than in the control at 0～24h after IL 1? or NS pretreatment( P 0 05). The results suggested that low dose IL 1? pretreatment might inhibit the expressions in the CAMs of myocardium during the late period of ischemia /reperfusion,resulting in delayed cardioprotection.

Objective To determine the characteristics of early cognitive dysfunction and N300 event-related potentials(ERPs)in patients with acute cerebral infarction(ACI).Methods Thirty patients with ACI and thirty normal healthy elderly people were studied.The two groups were examined with a picture recognition test and EEG waveforms were recorded.The ERPs were analysed statistically.The mini-mental state examination(MMSE) was used to evaluate cognitive function,and the results were correlated with the ERPs. Results The average MMSE scores of the ACI and control groups were significantly different.Reaction times(RTs)in the picture recognition test were(798.63±49.32)ms in the ACI group and(765.21±35.67)ms in the control group,a difference significant at the 5% confidence level.The average accuracy rates,(59.75±8.45)%and(65.26±9.28)%,were also significantly different.Average N300 ERPs the in the ACI group were significantly lower than those in the control group,and the average latencies were significantly longer.Both latency and amplitude in the ACI group showed a linear correlation with MMSE scores. Conclusion The ERPs of ACI patients have some clinical significance,and can be used as a scale-based assessment of cognitive function.

Aim To figure out whether Lonicera mac-ranthoides could induce hemolysis. Methods In vitro, macroscopic observation and spectrophotometry were used to observe whether the solutions of extracts from Lonicera macranthoides, MacranthoidinB and Dipsa-cosideB could induce hemolysis in 2% red cell suspen-sion of New Zealand white rabbits. And the three test-ed materials were prepared in concentration gradient of 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 mg· L - 1; and in vivo, mice were respectively treated with MacranthoidinB (0. 110 g·kg - 1 , 0. 055 g·kg - 1 ), DipsacosideB(0. 020 g·kg - 1 , 0. 010 g·kg - 1 ), ex-tracts (2. 275 g·kg - 1 , 1. 137 g·kg - 1 , crude drugs) once per day for 7 days, and all of the tested doses de-pended on the clinical doses. Then, RBC, RET and MCHC before and after administration were tested. Re-sults The hemolytic ratio in each treated group was below 5% in vitro. And in vivo, the three materials did not induce hemolysis and had no significant influence on RBC,RET and MCHC(P > 0. 05). Conclusions Extracts from flower bud of Lonicera macranthoides, MacranthoidinB and DipsacosideB, have not caused hemolysis in vivo and in vitro in this research.

Objective To observe the recent clinical efficacy of the sequential therapy hormone in the treatment of active rheumatoid arthritis.Methods In accordance with the principle of digital sheet,160 patients with active rheumatoid arthritis were randomly divided into the observation group and the control group,80 cases in each group.On the basis of methotrexate and leflunomide in both groups,the hormone sequential therapy was given in the observation group,but prednisone was given in the control group.The clinical efficacy of treatment after 1 week and 3 months were compared in two groups.Results In the observation group,the indicators in 7 d after treatment were significantly reduced,compared with untreated(t =19.90,7.63,14.73,7.58,6.84,14.09,all P ＜0.01),In the control group,three indicators of the duration of morning stiffness,joint tenderness index and joint swelling index in 7d after treatment were significantly reduced,compared with untreated (t =13.42,3.34,7.24,all P ＜ 0.01),Compared the indicators in the two groups in 7 d after treatment,there were statistically significant differences (t =13.07,4.92,10.51,5.23,5.74,15.03,all P ＜ 0.01).The indicators in the 3 months after treatment in both groups were signifi cantly decreased,buttherewasnosignificantdifferencebetweenthetwogroups (t =1.80,1.73,1.59,1.22,1.21,1.35,all P ＞ 0.05).The total effective rate was 80% in the observation group; but the rate was 75 % in the control group;there was no statistically significant difference in the two groups(x2 =0.57,P ＞ 0.05).Conclusion The sequential hormone therapy is an effective means for the treatment of active rheumatoid arthritis,by controlled the symptoms of rheumatoid arthritis effectively and alleviated the patient's condition.