Objective To study the relationship between carotid intima media thicknes(IMT) and cerebral infarction in patients with type 2 diabetes.Methods The IMT of the carotid artery was measured by high resolution B mode ultrasound, the relationships between carotid IMT and risk factors of cerebrovascular disease were evaluated.Results The serum levels of fibrinogen, total cholesterol and low density lipoprotein cholesterol were significantly higher in diabetic subjects with cerebral infarction than those in control group ( P

At present, Chinese medical field faces with an important problem of how to correctly handle the relationship between medical and scientific research. Academician Li Lianda advocates doctors doing scientific research under the premise of putting the medical work first. He points out that there are many problems in the process of doctors doing scientific research at present such as paying more attention to scientific research than medical care, excessively promoting building scientific research hospital, only paying attention to training scientific talents, research direction be flashy without substance, the medical evaluation system should be improved and so on. Medical, scientific research and teaching are inseparable because improving medical standards depends on scientific research and personnel training. But not all doctors need to take into account of medical treatment, scientific research and teaching in the same degree while not all hospitals need to turn into three-in-one hospital, scientific research hospital or teaching hospital. It must be treated differently according to the actual situation.
Altitude ; Biomedical Research ; education ; manpower ; trends ; Humans ; Physicians ; psychology ; statistics & numerical data

Objective To investigate the reliability of appraising biofilm of Klebsiella with silver staining method.Methods Apply improved flat culture to make biofilm of Klebsiella,adopting silver staining method and scanning electron microscopy to observe and appraise.Results Observation with general optical microscope after silver staining to appraise biofolm is identical with that using scanning electron microscope.Conclusions Silver staining method used to appraise biofilm of Klebsiella is reliable and simple.

Objective To investigate the common problems and countermeasure of using ultrasound-guided modified Seldinger technique in peripherally inserted central catheter (PICC) placement Method The clinical data of 140 patients who underwent PICC placement using ultrasound-guided modified Seldinger technique were retrospectively analyzed. Results The success rate of catheterization was 98. 6% among these 140 patients,with 135 (96. 4% ) succeeded after a single attempt and 5 (3. 6% ) after 2 attempts. Besides, 7. 1% (n = 10) had difficulty in inserting the guide wire, 3. 6% (n =5) had difficult catheteration, 2. 1% (n =3) met resistance when inserting catheter into ipsilateral subclavian vein, 2. 1 % (n = 3 ) experienced dystopia of inserting catheter into internal jugulular vein, and 1.4% (n = 2) experienced the bending of PICC in the superior vena cava and axillary vein. All of these problems were handled successfully. Conclusions The common problems of PICC placement include puncture failure, difficulty in inserting guide wire, and difficult catheteration. A good knowledge of these problems will help increase the success rate.

OBJECTIVE To discuss how to strengthen detail management to control hospital infection in operating room. METHODS The consciousness of controlling hospital infection,detail management as the key,integration of daily supervision examination with periodic inspection test and assessment,perfecting the detail management mechanism of hospital infection,and executing operation rules strictly were carried out. RESULTS The knowledge of hospital infection in staff members was improved,working efficiency raised,nursing level increased obviously,and the sterile operation infection rate dropped down. CONCLUSIONS Strengthening detail management is good for hospital infection control in operating room.

Objective To investigate the significance of the expression levels of humans telomerase reverse transcriptase(hTERT) gene in peripheral blood mononuclear cells with lung cancer patients. Methods From Mar.2002 to Feb.2005,the expression levels of hTERT were detected in 30 lung cancer patients and 15 normal humans by quantitative measurement with RT-PCR. Results The expression levels of hTERT mRNA in lung cancer patients (0.906?0.112) were significant as compared with those health controls(0.407?0.104). Conclusion It is effective to diagnose lung cancer by the quantitative measurement with RT-PCR for the expression levels of hTERT mRNA.

Objective evaluate the mechanism that bacteria growing in biofilm always resist to antimicrobial agents,and to provide the theoretical basis for selecting antimicrobial agents in clinic.Methods Model of klebsiella pneumoniae bacterial biofilm were built up with the modified flat-board method and identified with the method staining with AgNO3 and confocal scanning laser microscopy.We used imipenem to induce the ESBLs production of BF bacteria.ESBLs production was performed by the standard disk diffusion method.Results The isolation rate of ESBLs producting strains in klebsiella planktonically planktonically were 20.0%(8/40).The isolation rate of ESBLs producting strains in klebsiella biofilm were 42.5%(17/40).The isolation rate of ESBLs producting strains in klebsiella biofilm being induced by imipenem were 65.0%(26/40).The isolation rate of group A and group B,groupB and group C was different from each other significantly with the statistic method of ?2-test.Conclusion One of the main reasons that klebsiella and escherichia coli resist to antibiotics is the synergetic effect of BF and ESBLs.

Objective To investigate the relationship between IPF and IL 8.Method Apply ELISA method to determine IL 8 produced by PBMCs in 24 IPF patients and 15 healthy persons.Result IL 8 produced by PBMCs in PIF patients was (265 66?35 32)ng/L,which was higher than that in healthy group (180 09?32 46)ng/L(P

Objective To study the effects of ? carotene on rat liver fibrosis induced by carbon tetrachloride (CCl 4). Methods ① After being treated with CCl 4 for 3 weeks to induce the experimental rat liver fibrosis, rats were treated with ? carotene 250 000 U/kg body weight by oral feeding twice a week. The normal and the CCl 4 control group were also set. ② The liver pathological and ultrastructual change were studied. The content of rat liver hydrooxylproline, the expression of ? 2 (Ⅰ) collagen RNA and transforming growth facter ? 1 (TGF ? 1) were studied at the same time. Results In the treatment group, the liver fibrosis scores were fewer significantly than those in the CCl 4 group ( P

Objective To explore the levels of serum angiotensin Ⅱand their clinical significance on pheochromocytomas. Methods Fifty-eight patients were randomly divided into three groups: Group Ⅰwith normal blood pressure; Group Ⅱ are essential hypertension; Group Ⅲ are pheochromocytomas. The levels of serum angiotensin Ⅱ (ATⅡ) in each group and at eleven time points: were measured. Results The levels of serum ATⅡ of groupⅡand Ⅲ were significantly higher than those of groupⅠ(P