BACKGROUND: The development of hip joint in children with developmental dislocation of the hip (DDH) has been evaluated by X-ray plain film, which mainly presents Shen Tong's line continuity and epiphyseal nucleus position. There is still a lack of quantitative and objective evaluation methods. OBJECTIVE: To evaluate the rotation center and dislocation degree in DDH children by using three-dimensional (3D) computed tomography (CT).METHODS: Preoperative 3D CT was performed for 16 unilateral DDH from December 2010 to December 2014 in Tongji Hospital of Huazhong University of Science and Technology, with 4 males and 12 females, at the mean age of (4.42±2.59) years. There were 10 cases on the left side and 6 cases on the right side. 3D digital models were constructed by analysis. The 3D coordinate system was established with reverse engineering software. In 3D coordinatesystem, using inverse solution method of sphere fitting engineering, the rotation center of the acetabulum, the rotationalcenter of the femoral head, and the radius of ossification were constructed. Ossific radius ratio and dislocation lengthwere calculated. RESULTS AND CONCLUSION: (1) The acetabulum has the same point as the rotation center with the head of femur,and no significant difference in X, Y, and Z coordinates was detected (Px > 0.05, Py > 0.05, Pz > 0.05). However, it is notthe same condition in ipsilateral acetabular rotation center and femoral head rotation center, showing significant differences (Px=0.052, Py < 0.05, Pz < 0.05). (2) There were no significant differences in ossific radius between the healthy and affected sides (P > 0.05). The ossific radius was (21.37±4.42) mm and (20.14±3.14) mm on the healthy and affected sides of the femoral head (P < 0.05). (3) There was no significant difference in ossific radius ratio between healthy and affected sides (0.544±0.069 and 0.522±0.088; P > 0.05). (4) The dislocation length was 8.64-35.28 mm, mean (19.47±7.84) mm. (5) These findings suggest that 3D CT reconstruction can construct 3D digital models of DDHchildren. Thus, the accurate rotation center of the hip can be identified so as to precisely measure the dislocation length.

BACKGROUND:Studies have shown that the physical development potential is often associates with certain combinations of hand print features.
OBJECTIVE:To analyze the body shape and structure characteristics of national women’s handbal team athletes.
METHODS:Fifty national women’s handbal team athletes were col ected from Shanghai, Beijing, Tianjin and Anhui provinces and PLA. The body shapes of the athletes were measured by the Research Institute of National Sports Council, and the hand prints were col ected. The common factors of body shape and structure characteristics were analyzed with factor analysis method, including height, weight, forearm length, wrist circumference, hand length, hand width and index finger length and large-handedness index.
RESULTS AND CONCLUSION:The body shape and structure characteristics of national women’s handbal team athletes could be divided into four types:long trunk lean type indicator, hand skeleton and muscle fiber development level index, flexible quality indicators as wel as the alertness and mental level indicators. The long trunk lean type indicator was the main factor to affect body shape and structure characteristics of national women’s handbal team athletes.

A L-arginine-producing mutant UN100-12(SG~(r),AE~(r)) was derived from Corynebacterium glutamicum ATCC138761 by combination treatment with ultraviolet(UV) and N-methyl-N-nitro-N-nitrosoguanidine(NTG). It could accumulate 16.6g/L L-arginine in the medium containing glucose as carbon source and ammonium sulfate as nitrogen source, when it was culrured at 30℃ for 4 days. And also this mutant has good stability of descendiblity of L-arginine.

As one of the most serious hereditary neuromuscular disease, spinal muscular atrophy (SMA) is caused by the loss or mutation of survival motor neuron 1 (SMN1) gene. It leads to a decrease in the level of SMN protein and a consequent loss of alpha neurons and progressive muscle atrophy resulting in the progressive muscle weakness, the significant disability and the shortened lifespan. Up till now, only three drugs have been approved for SMA, including the gene therapy drug onasemnogene abeparvovec. The antisense oligonucleotide drug nusinersen and and the small molecule chemical drug risdiplam were briefly introduced. Some representative samples of the small molecule chemical drugs and antisense oligonucleotide drugs targeting SMN2 in the clinical trial or preclinical research phases were also reviewed.

Objective: To investigate the neuroendocrine properties of adrenalcortical tumors. Methods: We retrospectively reviewed the clinical data of 99 adrenalcortical tumor patients, who were treated in Changzheng Hospital form June 1999 to June 2005. Expression of neuron specific enolase (NSE), chromogranin A (CgA) and synaptophysin (Syn) proteins were examined by immunohistochemistry (S-P method) using monoclonal antibodies. The general data of patients, including the age, symptoms, laboratory findings, and pathological types, were collected and subjected to statistical analysis with SAS v6. 12 software. Results: The expression of all the above 3 proteins was found in adrenalcortical adenoma tissues, with the positive rate of NSE being 80%, the positive rate of CgA being 48.9%, and the positive rate of Syn being 75.6%; the positive rates in the adrenalcortical carcinoma tissues were 77.8%, 22.2%, and 77.8%, respectively; and those in the normal adrenal tissues was 20%, 0%, and 10%, respectively. The positive rates of 3 proteins in adrenalcortical tumors was significantly higher than those in the normal adrenal tissues (P

ObjectiveTo evaluate the relief of pain and daily activities in patients with osteolytic tumor of thoracic lumbar vertebrae by percutaneous kyphoplasty (PKP).MethodsA retrospective study was conducted to review 35 cases of osteolytic tumor of vertebrae treated with PKP.All patients had a refractories back pain while CT and MRI showed osteolytic changes in their vertebrae. The operation involved percutaneously inserting inflatable vertebral body and creating a cavity filled with bone cement.The visual analogue scales (VAS),WHO standards for pain relief and owestry disability index (ODI) were recorded after the procedures.The change of height in abnormal vertebrae and status of postoperative leakage of PMMA were also recorded.These data were followed up after 1 day,1 month and 6 months and analyzed via t student test.ResultsThe total 35 operations were done successfully.All patients got a conspicuous pain relief at 24 h after operation,and no spinal injury or compression was found.The X-ray showed that the vertebrates treated were filled well by cerment.There were significant differences at pain levels[(7.52±0.81)vs(4.31±0.32)],locomotor activity scales (ODI) [(69.45±0.87) vs (54.45±0.85)] and the height of vertebral bodies [(2.0±0.5) mm vs (2.7±0.4) mm] before and after operation (t value was -7.34,-5.32 and -3.36,respectively,P ＜ 0.05).At 6 months,follow up studies showed that clinic therapeutic effects were stabled for odynolysis and locomotor activity scales.The rates of pain relief in 24 h,1 month and 6 months were 80.0 ％ (28/35),85.7 ％ (30/35),82.8 ％ (29/35),respectively.ConclusionPKP is a single safe and effective way to treat osteolytic tumor of thoracic lumbar vertebrae.It can simply,quickly,and effectively relief the pain caused by osteolytic spinal tumor,recover height of the abnormal vertebra,and improve patient's life quality safely with less complications.

Objective To evaluate the efficiency of Ginkgo biloba extract 50 (GBE50) on learning memory ability and inflammatory response in hippocampus of natural aging mice, and explore the underlying anti-aging mechanisms.Methods 16-month-old ICR mice were randomly divided into three groups:model group, GBE50 low and high dose groups. 1 month mice were as normal group. The mice in GBE50 low and high dose groups received GBE50. The mice in the normal and model groups received solvent (1%CMC-Na+) by intragastric administration. The Morris water maze test and step-down test were used to assess behaviors of the mice. Immunofluorescent staining was used to detect the number of Iba-1 positive cells. The enzyme linked immunosorbent assay was used to determine the expression of TNF-α and IL-1β.Results Compared with normal group, escape latency and swimming distance in the Morris water maze test in the model group increased (P<0.05);latency shorted and error times decreased in the step-down test (P<0.05);the number of Iba-1 positive cell in the hippocampus CA1 in the model group increased considerably (P<0.01);TNF-α expression was in a general upward trend while IL-1β increased apparently (P<0.01). Compared with model group, escape latency and swimming distance decreased in the Morris water maze test in GBE50 high dose group;latency and the error times increased in the step-down test (P<0.05);the number of Iba-1 positive cells decreased (P<0.05). TNF-α expression showed a downward trend and IL-1β expression decreased in GBE50 low dose group (P<0.05). Conclusion GBE50 can delay aging and increase learning memory of natural aging mice.

Objective To investigate the surgery outcomes between patients with different recovery states measured by visual analog scores (VAS),explore the relationships among surgery outcomes,bone cement,and degrees of pain relief,and to find out the best combination of cement volume and cement distribution for Percutaneous kyphoplasty (PKP).Methods There were 220 patients with 220 vertebra,from January 2012 to January 2014,who received PKP in our hospital.Patients were divided into two groups,on the basis of different VAS.The epidemiological data,operative effect,and surgical complications were compared between two groups.Characteristic curve (ROC) was used to analyze the effect of bone cement dosage and distribution on the diagnosis of patients with different recovery state.The correlation was analyzed between the dose and distribution of bone cement and surgery outcomes.Moreover,logistic regression analysis was used to assess the safety of cement implantation.Results A total of 77 recuperators and 143 non-recuperators were included in the study.Two groups of patients in epidemiological data showed no significant difference.The surgery duration,bone cement injection dose,cement distribution,vertebral height restoration rate and improvement of kyphotic angle in the recuperator group were all significantly higher than those in the non-recuperator group.Whether small dose and large dose of bone cement volume and expanded the bone cement distribution area could significantly improve the patient of operation after the recovery rate and bone cement dose with extensive bone cement distribution area and large bone cement dose with limited bone cement distribution area had the same operation after the recovery effect (x2 =2.880,P =0.090).When the cement volume was constant,cement distribution was positively correlated with the restoration rate of vertebral height and improvement of the kyphotic angle (r2 =0.207,P ＜ 0.01;r2 =0.159,P =0.02).Conclusions The value of cement distribution is better than cement volume in relieving patient pain for diagnostic.For PKP,cement distribution above 0.49 with small cement should be suggested.