Objective To describe the clinicopathological features of histiocytic necrotizing lymphadenitis ( HNL). Methods Routine lymph node biopsies of 10 HNL cases were reviewed and their immunophenotyping were performed using S-P immunohistochemical staining. Results Histologically, HNL had discrete or integrated nodules variable in size in the paracortex,expecially in the interfollicular area,which were full of proliferating pleomorphic histocytes,transformed lymphocytes,and karyorrhectic debris without infiltration of the neutrophils, plasma cells and/or eosinophils. Immunohistochemistry revealed CD45RO + for transformed lymphocytes,CD68 +for histiocytes.and CD20 +for lymphocytes in the residual germinal centers. Conclusion The presence of pleomorphic histiocytes, transformed T-cells, and karyor-rhectic debris in the biopsy of lymph nodes, together with the absence of neutrophils support the diagnosis of HNL.

Objective To study the effects of panaxydol(PND) upon neurotrophic factors(NTFs) expression in Schwann cell,and to further reveal the mechanisms that underlie these effects. Methods Schwann cells were treated with PND of different concentrations in the culture medium.NTFs expression was examined through immunoiistochemistry assay.The intracellular cAMP level was assessed by radioimmunologic assay. Results There was an increase in NGF expression after PND treatment within the range of 2.5-20.0?mol/L(P

TIMI Flow as a method to assess myocardial reperfusion has been employed clinically ever since 1980's.TIMI Frame Count grading is a more objective,repeatable and correlative in variablity than those of TIMI Flow.TMBG and TMP provide the reperfusion evaluation on myocardial microvascular level.Doppler contrast echocardiography could be used as an additional index to assess vessel recanalization and myocardial reperfusion.Moreover,MCE,continuous ECG monitoring of ST-segment,isotope imaging of myocardial reperfusion and myocardial-enhanced MRI can all be used as non-invasive evaluating approaches with great clinical value.(J Intervent Radiol,2006,15:250-253)

Objective And effect of psychological intervention observation and analysis of iron combined with erythropoietin in the treatment of anemia in hemodialysis patients and the effect of. Methods 110 cases of Shaoxing Hospital of Traditional Chinese Medicine in anemic hemodialysis patients with January 2015 to February 2016 underwent diagnosis and treatment as the research object of the study, all the patients were randomly divided into 2 groups, respectively for the test group and control group, 2 groups of 55 cases. The control group was treated with erythropoietin receptor activator and psychological intervention treatment, experimental group was treated with ferrous succinate combined with erythropoietin receptor activator for treatment, but also psychological nursing intervention, observation and comparison of the symptoms of anemia, 2 groups of patients after treatment of adverse reaction etc. Results The experimental group patients with red blood cells, serum ferritin, hemoglobin and hematocrit were significantly higher than control group, the difference was statistically significant (P<0.05); side effects in test group than in the control group, the difference was statistically significant (P<0.05). Conclusion Iron combined with erythropoietin treatment in anemic hemodialysis was more effective at the same time, then the implementation of the corresponding psychological intervention, can reduce the adverse reactions in patients with symptoms, improve the safety of the treatment, and has high applicationvalue.

Objective To investigate the effect of hepatocyte growth factor(HGF)on the proliferation of human hepatocellualr carcinoma cells,and the mechanism of HGF-induced proliferation inhibition.Methods Human hepatocellualr carcinoma cell line HepG2 were treated with different concentrations of HGF for different time periods,and the proliferation of these cells was examined by colorimetric BrdU cell proliferation enzyme-linked immunosorbent assay.The expression of S-phase kinase associated protein 2(Skp2)was examined using Western blot and RT-PCR.Plasmids pcDNASkp2 was introduced into HepG2 cells,then the clones showing up-regulation of Skp2 were selected,and the effect of HGF on the proliferation in these clones was investigated.Results HGF inhibited the proliferation of hepatoma cells in a dose and time dependent manner.The expression of Skp2 was significantly suppressed by HGF.Furthermore,HGF did not suppress the proliferation of HepG2 cells transfected with Skp2.Conclusions This study suggests that HGF could inhibit HepG2 cell proliferation,and the down-regulation of Skp2 could be closely related to this suppressed proliferation.

Objective to observe the efficacy and application value of PFNA and Gamma in the treatment of intertrochanteric fractures in elderly. Methods 100 patients with intertrochanteric fractures were divided into PFNA group and Gamma group, who received PFNA treatment and Gamma treatment respectively. And the operation status of patients at different ages,postoperative recovery,and complications were observed. Results The operation time,intraoperatve blood soss of PFNA group were lower than those of Gamma group(P<0. 05). There was no significant difference in fracture healing time. The Harris score of the over 75s in PFNA group was higher than that in Gamma group(P<0. 05),and the complications were less than Gamma group(P<0. 05). Conclusion PFNA was suitable for the patients at differ-ent ages with the advantages of more rigid fixation,good anti-rotation,fewer trauma and fewer complications.

Fifty-two patients with silient myocardial ishcemia (SMI) were categorized into Type Ⅰ SMI group (n=21) and Type HI SMI group (n=31).22 normal subjects were also observed to serve as the control.It was found that the apparent viscosity of whole blood at different shear rate of 192.0 s-1,30.72s-1,and 3.84 s-1,the plasma viscosity,and the inter-viscosity of erythrocytes,and the duration of total myocardial ischemia on Holter monitoring electrocardiogram were higher and longer in Type Ⅲ SMI patients than in Type Ⅰ SMI ones and the normal controls (P

Objective:To evaluate the biocompatibility Abstract of a liquid of propolis. Methods:According to the relative Chinese national standards,the cytotoxicity of the propolis abstract was investigated by in vitro cell culture,agar diffusion test and the oral mucous membrane irritation response test in rabbits. Results:In the in vitro tests the propolis abstract showed grade 0 cytotoxicity. In the in vivo test the abstract did not result in any pathological change in oral mucous membrane.Conclusion:The propolis abstract is biocompatible.

Objective To study the liver damage induced by platelet activating factor (PAF) and the protective effects of the PAF antagonist WEB2170. Methods Experiments in vivo: after the administration of PAF to induce liver damage in rats, serum levels of aspartate transaminase (AST), alanine transaminase (ALT), lactic dehydrogenase (LDH) and tissue malonyldialdehyde (MDA) were determined by biochemical method, the succinate dehydrogenase (SDH) activity in mitochondrion was assessed by histochemical method, and the morphologic changes in hepatic structure were observed. In the treatment group, WEB2170 was used for intervention. Experiments in vitro: the fluidity of cellular velum of liver cells was detected with DPH probe, and the effect of TNF and MDA produced by Kupffer cells cultured by PAF stimulation was observed. Results PAF increased the expression of serum AST, ALT, LDH and tissue MDA (P

Objective To study the mechanisms and expressions of Fas/FasL in the lungs,spleens and lymph nodes of SARS patients.Methods The lungs,spleens and lymph nodes specimens of four autopsy cases and one control subject were studied with immunohistochemical mehod.Results Compared with normal tissues,apoptotic cells increased significantly in the spleens,lungs and lymph nodes of SARS patients.The apoptotic cells of lungs included alveolar epithelial ones,bronchial epithelial ones,monocytes/macrophages and lymphocytes.Apoptosis in the spleens and the lymph nodes was observed primarily in monocytes/macrophages and lymphocytes.Conclusion Over-expression of Fas/FasL is a general phenomenon in SARS,suggesting that Fas/FasL may play a key role in the pathogenesis of SARS.