Objective To observe if IL-2 gene can express in hepatic carcinoma cells by double targeting of hepatitis B virus envelope (HBVE) and alpha fetoprotein (AFP) promoter.Methods HepG2 cells,L02 cells,and HepG2.2.15 cells were cultured in vitro.HBVE was obtained by PEG8000 concentration assay,and the acquired HBVE was used to pack recombintional gene.Human AFP promoter-IL-2 recombinational gene was obtained by PCR.Then HepG2 cells and L02 cells were transfected by transient transfection and the expression of IL-2 was detected by RT-PCR and Western blot.Results IL-2 was detected in HepG2 cells by RT-PCR and Western blot but not in L02 cells.Conclusion By using HBVE as a gene transporter,human AFP promoter-IL-2 recombinational gene can express in hepatic carcinoma cells,thereby it can increase the safety of exogenous gene transfection of hepatic carcinoma cells.

Objective To observe the clinical efficacy of moxibustion plus Cang Gui Tan Xue (dark turtle seeking hole) needling method in treating cervical spondylosis of nerve root type. Method Sixty patients with cervical spondylosis of nerve root type were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by moxibustion plus Cang Gui Tan Xue needling method, and the control group was by simple needling method. The symptom and sign score was observed before and after 2-course treatment, and the clinical efficacy was compared. Result The total effective rate was 90.0%in the treatment group versus 83.3%in the control group, and the difference was statistically significant (P<0.05). After intervention, the symptom and sign scores were significantly changed in both groups (P<0.05). There was a significant difference in comparing the symptom and sign score between the two groups after intervention (P<0.05). Conclusion Moxibustion plus Cang Gui Tan Xue needling method is effective in treating cervical spondylosis of nerve root type.

Clinical communication skills are the one of seven lowest requirements of global medical education.This review emphasizes the importance of cultivating communication skills in general medical,indicates the defections of curriculum installation and discusses the training mode and examination mechanism.

Objective To introduce the possible effect of endogenous angiogenesis inhibitive factors in the therapy of hepatocarcinoma.Methods Recent relevant literatures were reviewed. Results Endogenous angiogenesis inhibitive factors can suppress the growth of tumor blood vessels,which might head off the development and metastasis of hepatocarcinoma effectively.This might provide a new approach to the therapy of hepatocarcinoma.Conclusion Recent studies on endogenous angiogenesis inhibitive factors will be helpful in the prevention and treatment of hepatocarcinoma.

Objective To observe the effect of pulsed electromagnetic fields (PEMFs) of different intensities on the biomechanical properties of the femur in ovariectomized rats so as to determine the intensity for the best therapeutic efficacy. Methods Fifty female Sprague-Dawley rats were randomly divided into (1) a sham-operated control group (no PEMF treatment) , (2) ovariectomized control group (no PEMF treatment) (3) ovariectomized group Ⅰ (PEMF treatment at 8 Hz and 0.77 mT intensity, 40 min daily for 30 days) (4) ovariectomized group Ⅱ (PEMF treatment at 8 Hz and 3.82 mT intensity, 40 min daily for 30 days) and (5) ovariectomized group Ⅲ( PEMF treatment at 8 Hz and 9.87 mT intensity, 40 min daily for 30 days). Except for the 10 rats of the sham-operated control group, all the others received a standard ovariectomy. Serum estradiol (E2) and the biomechanical properties of one femur (peak load, maximum displacement, maximum energy absorption, maximum stress, maximum strain and modulus of elasticity) were assessed after 30 days of PEMF treatment. Results In group Ⅱ the biomechanical properties of the femur were significantly better than in group Ⅰ or the ovariectomized control group. In groups Ⅰ and Ⅲ the biomechanical properties of the femur were not significantly better than in the sham-operated group. In group Ⅱ the biomechanical properties of the femur were significantly better than in groups Ⅰ or Ⅲ. Conclusion PEMFs at 3.82 mT can improve the biomechanical properties of the femur significantly.

Animals ; Genes, T-Cell Receptor ; genetics ; Humans ; Hypoxanthine Phosphoribosyltransferase ; genetics ; Mice ; Mutation

Sleep-wake disturbance is not only a risk factor for stroke,but also one of the serious complications of stroke.The main clinical manifestations of post-stroke sleep-wake disturbance are insomnia,arousal disorders,sleep-related movement disorders and parasomnias.Its mechanism is mainly associated with the sleep-wake center after stroke injury.

Objective To evaluate vagina reconstruction with the use of sigmoid colon. Methods 21 cases underwent vagina reconstruction with the use of sigmoid colon were reviewed of which 13 were male pseudohermaphrodism and 8 congenital devoid of vagina. Results The appearence of the reconstructed vagina has been good in all the 21 patients.15 of the 21 have been followed up for 3～24 months with no overproduction of mucus and no need of routine dilatation. Conclusions Vagina reconstruction with the use of an isolated sigmoid loop is an ideal procedure for juveniles and adults.