Objective: To study the e ects of Heying Anxin Fang on cardiac function and intracellular Ca2+ concentration in cadiocyte of chronic congestive heart failure rats. Methods: Animal models of heart failure were induced by subcutaneous injection of a large dose of ISO, and were randomly divided into model group, Shenmai injection group, captopril group, high, middle and low dosages of Heyinganxin Fang group and normal group with 9 in each group. To determine the indexes of LVSP, LVEDP, +dp/dtmax ,-dp/dtmax and heart weight, and determine Ca2+ concentration in cardiomyocytes by ow cytometer. Results: Compared with normal group, the indexes of heart weight, Ca2+ concentration and LVEDP in model group increased, but indexes of LVSP, +dp/dtmax, -dp/dtmax decreased obviously(P

Objective:To investigate methylphenidate response to different subtypes of attention deficit hyperactivity disorder(ADHD) boys by measuring the changes of the alpha competitive structure after administration of single dose of methylphenidate,explore the neuropathological mechanism and search for the sensitive index to predict methylphenidate response in this disorder.Methods:The study involved 88 ADHD boys(40 ADHD-C boys,48 ADHD-I boys) who met the DSM-Ⅳ diagnostic criteria of ADHD,and their continuous Electroencephalogram(EEG) data collected before and 2 hours after taking methylphenidate(10 mg) were analyzed with EEG-encephaloflutuographic technology(EEG-ET).Results:After taking methylphenidate,(1) The main frequency of alpha band obviously increased in ADHD-C boys and ADHD-I boys(from 9.05?0.96 Hz up to 9.72?0.99 Hz,P=0.000;from 8.90?0.93 Hz up to 9.25?0.86 Hz,P=0.002;separately),especially in ADHD-C boy group(Z=-2.111,P=0.035).(2) The dominant probability of 8 Hz was significantly decreased in ADHD-C boys and ADHD-I boys(from 24.34%?12.70% down to 20.74%?12.46%,P=0.002;from 28.82%?12.51% down to 25.64%?12.176%,P=0.003;separately).There was no significant difference in the changes of the dominant probability of 8 Hz between the two ADHD groups(Z=-0.494,P= 0.621).(3)The entropy value was significantly decreased in ADHD-I boys group(from 0.74?0.10 down to 0.70?0.13,t =3.579,P=0.001).There was no significant difference in the changes of the entropy value between the two ADHD groups(Z=-1.131 P= 0.258).Conclusion:After administra-tion of single dose of methylphenidate,slow ? wave decreased in ADHD boys,especially in ADHD-Cboy group;Single dose of methylphenidate can ameliorate the brain self-organization of ADHD-I boys.These findings suggest that the main frequency of alpha band may be the sensitive index to predict methylphenidate response in ADHD.

0.05).(2) The entropy value of the ADHD with ODD group was significantly higher than that of the normal controls in the right frontal region,right central region,and bilateral occipital region(P

Objective:To explore the alpha competitive structure in children with attention deficit hyperactivity disorder(ADHD)with/without learning disabilities(LD).Methods:According to DSM-Ⅳ diagnostic criteria,the study involved ADHD children with LD,pure ADHD children and normal controls.Each group consisted of 68 subjects.All subjects were between the ages of 7 and 14 years,and the groups were matched by sex,age and ADHD subtypes.EEG data were recorded during an eye-closed resting period and then were analyzed with EEG-encephaloflutuographic technology(EEG-ET).Results:(1)The pure ADHD children showed significantly more 8 Hz activity(25.84%?14.81%)than that of the normal control group(16.50%?11.42%,P=0.000);The main frequency of alpha band was 10 Hz in the pure ADHD children,while the energy distribution among alpha components was diffuse.(2)ADHD children with LD showed significantly more 8 Hz and 13 Hz activity(25.11%?11.88%,1.14%?1.14%,separately)than that of the normal control(16.50%?11.42%,0.74%?0.97%,P=0.000,P=0.009,separately);The dominant probability of 10 Hz(27.80%?13.28%)in this group was significantly lower than that of the control group(36.06%?17.21%,P=0.011);The energy distribution among alpha components was diffuse in ADHD children with LD,whose main frequency of alpha band was 9 Hz;The entropy value of the ADHD children with LD was significantly higher than that of the control group in the right brain and the left parietal region,temporal region,occipital region(P

OBJECTIVEThis review aims to identify the dimensional structure of the shortened dentine hypersensitivity experience questionnaire (DHEQ-15) using confirmatory factor analysis (CFA).

METHODSThe Chinese language version of DHEQ-15 was applied to 210 patients with dentine hypersensitivity aged 18-70 years old. The three-dimensional construct was confirmed by CFA.

RESULTSAll the questionnaires were fully completed. The item "Because of the sensations, I take longer to finish a meal than others" was the most frequently reported by the parents. The modified three-factor model is suitable for the data. The chi-square fit statistics/degree of freedom was 2.213, the goodness-of-fit index was 0.905, the adjusted goodness-of-fit index was 0.872, the normed fit index was 0.845, the incremental fit index was 0.962, the Tucker-Lewis index was 0.969, the comparative fit index was 0.977, and the root mean square error of approximation was 0.068.

CONCLUSIONSOur findings generally support the three-dimensional construct of the DHEQ-15. Thus, DHEQ-15 can be used as a valid instrument in patients with dentine hypersensitivity in China.

With the development of genome-wide sequencing technology, 195 types of functional, long non-coding RNAs (lncRNAs) have been identified so far, and their cellular roles are gradually being revealed. LncRNAs have now become a hotspot in the field of life sciences. These small molecules exist in almost all higher eukaryotes and play very important regulatory roles in these organisms. This review briefly summarizes the recent progress in research on plasmacytoma variant translocation 1 gene (PVT1), an lncRNA.

A new experiment curriculum system of environmental microbiology was established centering on applied microbiology in environmental protection field and emphasizing on design and research experi- ments to motivate the students’ interests for the course, which helped them to improve their ability of think- ing independently and creatively as well as their practicing ability.

ObjectiveTo determine the risk factors and the optimal management of hepatic artery complications (HAC) after orthotopic liver transplantation.MethodsThe clinical data of 180 orthotopic liver transplantation patients performed between January 2005 and September 2007 was reviewed.The incidence of HAC between primary liver carcinoma and benign diseases of liver was compared.ResultsTwelve (6.7％) episodes of HAC were identified.3 were hepatic artery thrombosis (HAT) and 9 were hepatic artery stenosis (HAS).The incidence of HAC in patients with primary liver carcinoma (6/39) was higher than benign disease (6/141)(P＜0.05).ConclusionsThe keys to management of HAC after orthotopic liver transplantation are to diagnose the complication in time and to select the proper treatment based on the type of HAC.

Objective To observe the effects of individual occupational therapy on cognitive impairment of elderly with stroke. Methods 60 elderly stroke patients with cognitive impairment were divided into treatment group (n=30) and control group (n=30). All the patients accepted routine rehabilitation, and the treatment group accepted individual occupational therapy in addition. They were evaluated with Mini-mental State Examination (MMSE) and modified Barthel index (MBI) before and after treatment. Results The score of MMSE increased significantly in the treatment group after treatment (P<0.001), but was not different significantly in the control group (P>0.05). The score of MBI increased significantly in both groups after treatment (P<0.05), and increased more in the treatment group than in the control group (P<0.001). Conclusion Individual occupational therapy can improve the cognitive function and the activities of daily living for the elderly stroke patients with cognitive impairment.

ObjectiveTo explore the prevalence and spectrum of β-thalassemia mutations in Fujian province,and to provide a reference for prenatal diagnosis and genetic counseling in this population.Methods Two thousand three hundred and one blood samples were randomly selected from 9 different areas of Fujian province from May 2008 to December 2010.PCR and reverse dot blot hybridization (RDB) were adopted for detection of the 17 common types of mutation,and the frequency of each genotype of β-thalassemia mutations was calculated.The β-globin gene of unknown positive samples were analyzed directly with DNA sequencing.Results Three hundred and fifty-nine cases were detected with β-thalassemia mutations of the 2301 copy blood samples submitted,and the detection rate was 15.60％ (359/2301).Of the mutated genes,12 different mutations were identified,namely IVS-2-654(C→T),CD41-42(-TCTT),CD17(A→T),-28(A→G),CD27-28(+C),CD26(G→A),CD71-72(+A),IVS-1-1(G→T),CD43(G→T),-29(A→G),initiation codon ATG→AGG and CD36(-C).Mutation frequencies were 46.54％ (175/376),33.24％ (125/376),9.31％ (35/376),5.05％ (19/376),2.13％(8/376),1.33％(5/376),0.80％(3/376),0.27％(1/376),0.27％(1/376),0.27％(1/376),0.53％(2/376),and 0.27％(1/376),respectively.The most common mutations were IVS-2-654 (C→T) and CD41-42 (-TCTT),which accounted for 79.78％(300/376) of total genetic mutations.In addition,a novel β-globin gene mutation CD36 (-C) allele was detected for the first time,the deletion of a nucleotide C at code 36 within exon 2 lead to a frameshift mutation that could result in a premature termination at code 60.Conclusions β-thalassemia mutations in Fujian province are complex with significant genetic heterogeneity.We present for the first time the detection of a new β-thalassemia mutation in the population:CD36(-C),which provides valuable information for genetic counseling and prenatal diagnosis in Fujian province.