Cervical cancer is the third leading cause of cancer death among females in less developedcountries. Drugs used in the treatment of cervical cancer were reported to exert a cytotoxic effect on thenormal cells. This study aimed to determine the effectiveness of Quercus infectoria (QI) vaginal creamtowards cervical cancer cell, HeLa and its toxicity effect on the female rat model. Methods: MTT assayswere utilized to determine the median concentration (IC50) for cell cytotoxicity of QIA and QI vaginal creamagainst cervical cancer cells, HeLa. Expression of HPV E6 and E7 protein in HeLa cells treated with QI vaginalcream for 24 hours were conducted by Western blot analysis. In separate experiments, the toxicity of QIvaginal cream on a lower reproductive tract of the female rat model has been assessed by histopathologicalexamination after application for three weeks. The antioxidant activity of QIA extract and QI vaginal creamwere assessed by DPPH radical scavenging assay. Results: A moderate cytotoxicity activity exerted by QIAextract and QI vaginal cream against HeLa cell with IC50 values of 13.90 ± 2.27, and 20.80 ± 1.94respectively. Furthermore, QI vaginal cream suppressed the expression of HPV E6 and E7. Daily applicationof QI vaginal did not exert any inflammation to the vaginal mucosa and cervix. QIA extract and QI vaginalcream demonstrated high DPPH radical scavenging activity. Conclusion: Formulated QI vaginal cream hascytotoxic effect on HeLa cells without causing an adverse effect on the lower reproductive tract in femalerat mode