No abstract available.
Haplorhini ; Humans

Background: Group A Rotavirus is the main cause of diarrhea in human especially in children under 5 years old. Rotavirus master seeds were established from group A Rotavirus (mainly 3 strains: G1P8, G1P4 and G4P6) causing acute diarrhea for children in Vietnam. The master seeds must meet the potency and safety in the laboratory as well as animal experiments under the guidance of WHO. Objectives: To determine the safety and immune response of rotavirus master seeds in monkeys to confirm their safety and effect in preclinical stage. Subjects and method: Baby Macacca mulatta monkeys had average weight of 1.5 kg (provided by monkey ranch in Reu island in Quang Ninh province) were tested and determined neutral antibody by immunofluorescence technique. Results: The rotavirus master seeds: G1P8 (KH0118); G1P4 (2001019210) and G4P6 (2001019203) had good safety and immune response with high neutral antibody after 3 dose vaccination in baby Macacca mulatta monkeys. Conclusion: The rotavirus master seeds would be a base for diarrhea vaccine production in Viet Nam under the guidance of the World Health Organization.
Rotavirus/ immunology ; Haplorhini

Trypsinization method in our modification was harvested high yields of dissociated cells. The total of cells is obtained 19.47 X 108 per pair of monkey kidney weighted 10 gram. Whereas rate of available cell reach 83.9% and rate of single cell and small group (<5 cells) collected was about 90%.
Trypsin ; Haplorhini ; Kidney ; Cells

Chromosomes of 45 samples of fetal monkey kidney cells from primitive to 9th passages was examined. Results showed the quantity of 2n = 42 chromosome reached 89,86-96%. The polyploidy phenomenon including internal polyploidy trended to increase from the 4th passage and the most number of chromosomes was obtained from 6th to 9th passage. In every passage, both numeral mutation and aberration were at normal range
Chromosomes ; cells ; Haplorhini ; Kidney

At the dose of 4 and 8 mg/kg/day for 30 consecutive days, the monkeys appeared to have normal living actions taking meals and drink. Their body weights did not change significantly. At the dose of 4mg/kg/day for 30 consecutive days, changes of SGOT and creatinine indices were not significant but erythrocyte, reticulocyte, leucocyte and SGPT indices changed significantly. These indices became normal 15 days after interruption of the drug administration. At the dose of 8 mg/kg/day for consecutive 30 days, the changes of SGOT index was not significant during the administration. Haemoglobin, erythrocyte, reticulocyte, leucocyte, SGPT and creatinine indices were significantly changed during the 30 days administration. Haemoglobin, erythrocyte, reticulocyte, SGPT and creatinine indices changed significantly but erythrocyte and leucocyte indices became normal 15 days after the drug administration
Toxicity ; animals ; Haplorhini ; Artemisinins

Aquaporin (AQP) is a water channel protein that is of critical importance in the urinary concentrating process and the regulation of water balance in the kidney, and at least seven AQPs are expressed at distinct sites in the kidney. The common marmoset monkey is widely used as an experimental animal included in the primate order in the filed of renal system. However, nothing is known about the expression AQP in the common marmoset monkey kidney. The purpose of this study was to establish the distribution of AQP-1, AQP-2, AQP-3 and AQP-4 in the common marmoset monkey kidney. We used three male common marmoset monkeys (Callithrix jacchus) ranging in age from 2 to 3 years. AQP-1 was expressed in segments 1, 2 and 3 of the proximal tubule, particularly abundant in segment 1, and also observed in the descending thin limb of the medulla. AQP-2 immunoreactivity was observed in the apical plasma membrane of principal cells in the cortical and medullary collecting ducts. AQP-3 immunostaining was intense in the basolateral plasma membrane of connecting tubules as well as in the cortical and outer medullary collecting ducts. AQP-4 was expressed mainly in the cytoplasm of inner medullary collecting duct cells. These data suggest that AQPs of the common marmoset monkey kidney may play a similar role in urinary concentrating processes and the regulation of water balance to that of AQPs in rats, mice and humans.
Animals ; Aquaporins* ; Callithrix* ; Cell Membrane ; Cytoplasm ; Extremities ; Haplorhini* ; Humans ; Immunohistochemistry ; Kidney* ; Male ; Mice ; Primates ; Rats

To assess the safety of playground equipments in the elementary schools of Taegu, a site visit was made to 117 elementary schools out of 119 schools between 1st and 30th April 1991. Safety criteria were developed on the bases of Massachusetts' Playground Safety Check List, standard height of Korean children of 6-12 years old, and source book for designing playground equipments. There were eleven different kinds of playground equipments installed in 117 schools but the number of equipments was about 50-60% of the minimum requirement set by the Ministry of Education except chin-up bar. Among the installed equipments, 47.3% of swings, 16.6% of parallel bars, 20.0% of monkey bars, and 16.0% of slides were broken down. None of the swings, slides, seesaws, monkey bars, and sandboxes met the safety criteria to the full but 59.0 of chin-up bars, 31.4% of parallel bars, and 13.5% of stumps met the criteria fully. The proportions of equipments that were dangerous for children to play on were 26.4% for slides, 20.0% for monkey bars, 11.6% for seesaws, 10.4% for parallel bars, 9.85 for sandboxes, 7.4% for swings and stumps, and 3.9% for chin-up bars. The rests were either in need of repair of broken down. It was revealed by this survey that the playground equipments were too short in number to meet the minimum requirement, designs and size were not standardized, and many of the equipments were involved with the risk of child accidents. Therefore, a safety standard for the playground equipments should be developed and the existing equipments should be repaired immediately.
Child ; Daegu* ; Education ; Haplorhini ; Humans

Acute toxicity of injectable doses of 40mg/kg, 80mg/kg, 120mg/kg, 160mg/kg of BB101 (trifluoromethyl hydroartemisinine) corresponding to 1/10,1/5, 1/4, 1/3 of LD50 by injection on monkey was studied. With the doses of 40mg/kg and 80mg/kg, on monkey, normal living activity was still manifested except anorexia, weight loss, hair fall...Bad conditions had been aggravating with the increasing doses. At the dose of 120mg/kg, monkey died 2 days after interruption of toxic substance and at the dose of 160mg/kg, monkey died on the day N5.As regards biochemistry indices, BB101 in 5 consecutive days with the daily dose of 40mg/kg and 80mg/kg enhanced SGOT, SGPT, proteine and creatinine (in permitted intervals);with 120mg/kg and 160mg/kg enhanced SGOT and SGPT by 3-5 folds higher, other indices were in permissible intervals. As regards hematological indices; white blood count, red blood count and haemoglobine reduced
toxicity ; Haplorhini ; animals ; Animal Experimentation

The study on using dispase and trypsine 0.25% to detach cells from fetal monkey kidneys of Macaca mulatta showed that: the detach ability of dispase was much higher than trypsin. Some characteristics of dispase were suitable for detaching fetal cell in cold condition or serum condition. However, dispase has not been used as commonly as trypsin. Dispase can not be a good solution to detach cells without the effect of trypsin
Endopeptidase ; cells ; Kidney ; Haplorhini ; Fetus

A coxsackievirus B strain was successfully isolated by cells culture from cardiac muscle tissues of a dead Sichuan golden monkey with myocarditis from a zoo of Changchun in China. The isolate was consistent with CVB by morphology, physicochemistry test, animal regression test and RT-PCR. Analysis of VP1 partial gene sequence and detection of mice specific serum IgG showed that the strain isolated was a coxsackievirus B3. It was the first CVB case report in Sichuan golden monkey and the strain isolated was named CVB/SGM-05.
Animals ; Cercopithecus aethiops ; Enterovirus B, Human ; isolation & purification ; Haplorhini ; virology ; Heart ; virology ; Mice ; Reverse Transcriptase Polymerase Chain Reaction ; Vero Cells ; Viral Structural Proteins ; genetics