Oct4 gene,a member of POU transcription factor family,is expressed in embryonic stem(ES) cells and embryonic carcinoma(EC) cells,but not in cells of differentiated tissues.It plays a critical role in maintaining pluripotent and self-renewing state of embryonic stem cells.The molecular structure,function,regulation passageway and the relationship with tumors of Oct4 gene have been briefly reviewed.

Objective To study the expression of Oct4 gene, an embryonic stem cell marker, in cervical carcinoma cell lines and tissues for the purpose of finding the relationship between this gene and cervical cancer. Methods The expression of Oct4 in normal cervical tissues and cervical carcinoma tissue and cell lines was detected by the immunohistochemistry and RT-PCR. Results ① The expression of Oct4 mRNA in cervical carcinomas (73.3%, 22/30) was significantly higher (P<0.01) than that in normal cervical tissues (25%, 3/12); ② The rate of Oct4 protein expression in cervical cancer (53.7%, 22/41) was higher (P<0.01) than that in normal cervical tissues (7.7%, 1/13); ③ Oct4 protein was expressed in cervical carcinoma cell lines and primary tumorspheres. ④ The expression of Oct4 in tumorspheres was down-regulated during cell differentiation. Conclusion Our results imply that Oct4 may be one of the cervical cancer stem cell markers, but this still needs further research data to support.

Objective:To investigate the application of PDCA cycle management combined with "three-step" bedside teaching in respiratory nursing teaching.Methods:A total of 80 nursing students who studies in Department of Respiratory Medicine, Beijing Chaoyang Hospital Affiliated to Capital Medical University, were included as subjects, and they were divided into control group and observation group using a simple random number table, with 40 students in each group. The students in the control group received traditional teaching, while those in the observation group received PDCA cycle management combined with "three-step" bedside teaching. The two groups were compared in terms of the scores of theoretical knowledge, operation skills, and physical examination, comprehensive clinical nursing ability, and degree of satisfaction with teaching. SPSS 22.0 was used to perform the chi-square test and t-test. Results:The observation group had significantly higher scores of theoretical knowledge, operation skills, and physical examination than the control group ( P<0.05). Compared with the control group, the observation group had significantly higher scores of nursing consultation, physical examination, diagnosis, nursing measures, health consultation, humanistic care, organizational effectiveness, and overall evaluation ( P<0.05). The observation group had a significantly higher degree of satisfaction with teaching than the control group ( P<0.05). Conclusion:PDCA cycle management combined with "three-step" bedside teaching in respiratory nursing teaching can improve the assessment scores of theoretical knowledge, operation skills, and physical examination among nursing students, enhance their comprehensive clinical nursing abilities, and increase the degree of satisfaction.

Objective To investigate the clinical efficacy of drug eluting stents vs metal bare stents for femoral popliteal arteriosclerosis obliterans.Methods The clinical data of 47 patients with femoral popliteal arte-riosclerosis obliterans receiving endovascular therapy from October 2020 to June 2021 were retrospectively ana-lyzed.A total of 24 cases received drug-eluting stents(DES group)and 23 cases underwent metal bare stents(BMS group).Results All patients successfully completed the operation without any adverse events.There was no statistical difference in ABI between the DES group and the BMS group at 7 days and 3 months after surgery,and ABI growth value of the DES group was higher than that of the BMS group at 6,12 and 24 months after surgery(P<0.05).There was no significant difference in the primary patency rate at 6 months after surgery,However,the primary patency rate in DES group was higher than that in BMS group at 12 and 24 months after surgery(91.7%vs.65.2%,83.3%vs.56.5%,P<0.05).For the target lesion revascularization rate of the two groups,DES group had a significant advantage over BMS group(4.0%vs.26.1%,P<0.05).Conclusion DES had better clinical efficacy and advantage over bare metal stent for the treatment of femoral popliteal arteriosclerosis obliterans.