Objective To investigate the effect of protection motivation intervention on patients with diabetic cataract after implantable collamer lens implantation. Methods A total of 118 diabetic cataract patients were divided into 2 groups (59 cases each) by random digits table method. The control group received routine ophthalmic nursing and the observation group was given protection motivation intervention based on the routine nursing. The change of the plasma glucose, postoperative visual acuity and the quality of life were evaluated. Results The blood glucose on the morning of the operation day and one day after operation in the observation group was (8.27±1.25) mmol/L, (9.52±2.44) mmol/L, which was significantly lower than that of the control group, (10.49 ± 1.48) mmol/L, (12.77 ± 3.36) mmol/L, the difference between the groups was statistically significant (t=-5.392,-6.755, P<0.01). After 1 month and 3 months, the vision of the observation group was 0.64±0.22, 0.67±0.30, which was higher than that of the control group 0.56± 0.18, 0.58 ± 0.20, in which the postoperative blindness or low vision rate was 3.95%(3 eyes) in the observation group, and 13.51%(10 eyes) in the control group(t/χ2=2.492, 2.209, 4.013,P<0.05). The quality of life in the observation group was 7.70±1.13 and 5.22±1.48 in the control group, there was statistical significance between the two groups(t=3.660, P < 0.05). Conclusions Application of protection motivation intervention on diabetic cataract patients can improve their postoperative vision and quality of life.

Measurement of intraocular pressure is a crucial task of ophthalmic nursing and it is important for diagnosing or curing ophthalmic diseases. In recent years,an increasing number of studies concerning technologies of monitoring intraocular pressure bring with us many new-type tonometers. In comparison with traditional tonometers,the new-type tonometers has made a progress in operative difficulty index,portability and accuracy. However,the application of these new-type tonometers in clinic also puts more challenges for ophthalmic nursing and ophthalmic nurses. Only by a comprehensive understanding of the working principle and application scope of different tonometers,could we measure intraocular pressure better and more accurate. For this reason,working principles,benefits and disadvantages of tonometers applied in clinic are reviewed as follow in order to better guide the ophthalmic nurse in clinical used correctly.

OBJECTIVE: To observe the dynamic changes of hematopoietic reconstitution and multiple lineages differentiation at early phase after transplantation. METHODS: Whole bone marrow mononuclear cells (wBMMNC, 5×10) and enriched c-Kit hematopoietic stem/progenitor cells (HSPC, 3×10) from the BM of B6-Ly5.1 mice were transplanted into lethally irradiated B6-Ly5.2 mice, the frequencies and absolute numbers of donor-derived cells (including LKS and LKS) were detected by flow cytometry. The multiple lineages differentiation of donor-derived cells was also monitored by flow cytometry. The homing and early phase proliferations of donor-derived cells were observed by two-photon microscope. RESULTS: The donor-derived cells started to proliferation from 5-7 days after transplantation and reached the peak value at 2-3 weeks after wBMMNC transplantation. The donor-derived cells proliferated from 1-2 weeks and maintained until 4 weeks after c-kitHSPC transplantation. At 1 week after transplantation, the donor-derived cells mainly differentiated into myeloid cells with a few lymphoid cells production (B cells) but the production of T cells was not observed at most in wBMMNC transplanted group, while myeloid cells occupied the majority of donor-derived cells at 2-4 weeks; donor-derived cells almost totally differentiated into myeloid cells at 1-3 weeks after transplantation in c-Kit transplanted group and donor-derived B cells appeared at 4 weeks. The absolute number of donor-derived LKS and LKS cells in the BM of c-Kit transplanted group were much higher than that of wBMMNC group (P＜0.001) at 2 weeks respectively. The clustering proliferation of cKit cells at 4-5 days after transplantation was observed by two photon microscope. CONCLUSION The dynamical rate of proliferation and reconstitution of donor-derived cells are much earlier and quicker in c-Kit group than those of wBMMNC group. c-Kit cells mainly differentiate into myeloid cells within 1-3 weeks and the lymphoid cell differentiation starts at 4 weeks after transplantation. The immediate proliferation and differentiation of c-Kit cells within 1 week maybe due to the urgent needs of hematopoietic regeneration under the myeloablated hosts.
Animals ; Bone Marrow Transplantation ; Cell Differentiation ; Cell Proliferation ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells ; Mice ; Mice, Inbred C57BL