Objective HBV DNA with two pairs of semi-hepatitis B treatment in the role.Methods ECLIA assay of serum hepatitis B two pairs of semi content was detected by PCR serum HBV DNA concentrations.Results Three positive-HBsAg,HBeAb,HBcAb (Ⅰ group) in patients with HBV DNA positive rate is 61.3％,Three positive-HBsAg,HBeAg HBcAb (Ⅱ group)in patients with HBV DNA positive rate is 84.3％,The positive rate of HBV DNA is 33.3％ in Ⅲ patients,Ⅳ groups of patients without HBV DNA positive.Conclusion HBV DNA with two pairs of semi-diagnosis and treatment for hepatitis B have complementary roles that can guide hepatitis B treatment.