Objective To analyze risk factors related to nosocomial infection in severe drug rash patients, in order to take nursing countermeasures to reduce the incidence of nosocomial infection in the future during nursing work. Methods A total of 158 severe drug rash patients hospitalized in Dermatological Department the First Affiliated Hospital of Wenzhou Medical University from January 2012 to March 2014 and were selected for the retrospection of general information, clinical treatments condition and analyzed with statistical methods. Results A total 18 of 158 severe drug rash patients were infected during hospitalized with infection rate of 11. 4%. Albumin levels, ulcer of skin mucous membrane, invasive operation, and diabetes were related risk factors to cause hospital infection (P<0. 05), but age, gender and hemoglobin levels were not (P > 0. 05). With logistic regression analysis, albumin levels less than 30g/L, ulcer of skin mucous membrane, invasive operation, and diabetes were severe drug rash patients′independent risk factors to hospital infection (P<0. 01). Conclusions Severe drug rash patients have multiple risk factors to hospital infection, so the countermeasures should be taking in clinical in order to decline the incidence of hospital infections.

This study aims to investigate the function of lmo2363 gene in stress resistance of Liste-ria monocytogenes strain LM83-1.In this study,the lmo2363 gene deletion strain and complement-ation strain of Listeria monocytogenes were constructed using overlapping extended PCR and ho-mologous recombination techniques,and the growth ability,stress survival rate and biofilm forma-tion ability of wild,deletion strain and complementation strain were compared under different stress environments.lmo2363 gene deletion strain and complementation strain of Listeria monocy-togenes were successfully constructed in this experiment.The growth curves showed that the growth capacity of the deletion strain was weaker than the wild strain LM83-1 under 4 ℃,7％NaCl,10％NaCl,3.5％ethanol,4.0％ethanol and pH5 stress(P＜0.001).The results of stress survival test showed that the survival rate of the deletion strain was significantly lower than the wild strain after 1 h treatment with pH3 and 10 mmol/L H2 O2 stress(P＜0.010).The biofilm forming ability of the deletion strain was decreased compared with that of the wild strain(P＜0.050).This study confirmed that lmo2363 gene mediated the adaptation of LM to low temperature,high osmotic pressure,ethanol and acid stress environment and affected the formation of LM bio-film.This study laid a foundation for further exploring the function of lmo2363 gene in the stress resistance process of Listeria monocytogenes.