1.Comparison of placement of peripherally inserted central catheters using vascular ultrasound guidance system and traditional method in 938 tumor patients
Yongzhi QI ; Yan GUO ; Xiaoxia XU ; Hao ZHANG ; Li LI
Chinese Journal of Clinical Nutrition 2012;20(4):253-255
Objective To compare the placement of peripherally inserted central catheters(PICC)by using vascular ultrasound guidance system and traditional method.Methods Totally 938 patients undergoing PICC were divided into the traditional method group and the vascular ultrasound system and microintroducer techniques group according to the puncture time.The differences in the one-attempt success rate and the overall success rate of the placement and the incidences of complications were compared between the two groups.Results The one-attempt success rate was 93.01% in the traditional method group and 98.76% in the vascular ultrasound system and microintroducer techniques group(P =0.005).The successful rate of PICC placement was 100%.The incidence of complication was 11.29% in the traditional method group and 2.47% in the vascular ultrasound system and microintroducer technique group(P =0.000).Conclusion The PICC placement using vascular ultrasound guidance system and microintroducer techniques can increase one-attempt success rate and decrease complications.
2.Study on pharmacologic action characteristics of traditional Chinese medicines distributed along liver meridian based on medicinal properties combinations.
Hong-Ling GUO ; Hao GU ; Yun WANG ; Yan-Jiang QIAO
China Journal of Chinese Materia Medica 2014;39(13):2409-2412
OBJECTIVETo establish a characterization system of traditional Chinese medicinal properties in line with modern scientific cognition regularity, in order to reveal properties of traditional Chinese medicines distributed along liver meridian and relations of effects of medicinal properties.
METHODBy collecting data about traditional Chinese medicinal properties recorded in the Pharmacopoeia of the People's Republic of China (2005 Edition), literature and data about pharmacological effects of traditional Chinese medicines recorded in the Chinese Materia Medica, by using the method of association rules, the authors dug pharmacological effect rules corresponds to relevant medicinal property combinations, with the medicinal property combination of traditional Chinese medicines distributed along liver meridian as the target.
RESULTIt was found that either obvious different pharmacological effects or identical pharmacological characteristics existed in traditional Chinese medicines distributed along liver meridian.
CONCLUSIONWith the aim to explore the correlations between traditional Chinese medicine medicinal properties and pharmacological effects, the authors linked the traditional Chinese medicine theory with modern research achievements, in order to provide the ideas and methods for interpreting mechanisms of medicinal properties.
Databases, Factual ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Humans ; Liver ; drug effects ; Liver Diseases ; drug therapy ; Meridians
3.Optimization of Saccharomyces cerevisiae sp.Strain by1.1b Culture Conditions for Efficient Biosynthesis of D-(-)-mandelate Dehydrogenase
Fen YAN ; Qian WANG ; Zi-Lin LIN ; Yang-Hao GUO ;
Microbiology 1992;0(01):-
The culture conditions of Saccharomyces cerevisiae sp.strain by 1.1 b were optimized for the production of D-(-)-mandelate dehydrogenase which is useful for the asymmetric bioreduction of benzoylformate to form D-(-)-mandelate.The optimum medium(per liter)consistes of 60 g peptone,30 g maltose, 0.5 g MgSO_4,0.01 g ZnSO_4,1.0 g KCl.After optimization of the culture medium,the enzyme production in shake flasks is enhanced from 2.56 to 20.21 U/L.The optimum fermentation conditions were determined as follows:medium volume 100 mL(i.e.,40%for a 250-mL shake flask),pH 6.5,inoculum size 10%,temperature 30℃,and cultivation time 25 h.
4.Chronic kidney disease in 5 708 people receiving physical examination
Guo XU ; Zhiheng CHEN ; Hao ZHANG ; Ni GONG ; Yan WANG
Journal of Central South University(Medical Sciences) 2014;(4):408-415
Objective: To investigate chronic kidney disease (CKD) and its risk factors in people receiving physical examination. Methods: hTis retrospective study included people over 20 years old who had physical examination in the Health Management Center of Third Xiangya Hospital from Janurary 2008 to June 2011. CKD and its risk factors as well as questionnaire were recorded. hTe risk factors were analyzed by multivariate logistic analysis. CKD was deifned by kidney damage (microalbuminuria≥30 mg/L) and/or hematuria and/or reduced kidney function [evaluate glomerular ifltration rate (eGFR)<60mL/(min.1.73 m2)]. We counted eGFR according to the modiifcation of diet in renal disease (MDRD). Results: A total of 5 708 physical examination reports were included. The detection rate of albuminuria, reduced renal function and hematuria was 25.0%, 1.7% and 1.1%. hTe detection rate of CKD was 25.6%, and detection rate of CKD stage 1-5 was 17.8%, 6.7%, 1.1%, 0 and 0, respectively. Multivariate logistic analysis indicated that diabetes mellitus, hypertension, hypercholesterolemia, male, age, and smoking were the risk factors for CKD. Increasing physical activity was the protective factor against CKD. Conclusion: High prevalence of CKD in people receiving physical examination is found in Changsha, especially stage 1 and 2 CKD. Physical examination is important to screen CKD. Stopping smoking, control of blood glucose, blood pressure, blood lipids and increasing physical activity may help reduce the prevalence of CKD.
5.Association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women:A Meta-analysis
Hao ZHANG ; Lu GAN ; Yan GUO ; Ruiheng WANG
Chinese Journal of Tissue Engineering Research 2013;(37):6695-6700
BACKGROUND:Genetic studies have shown that there may be association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women, but there are some differences in the conclusions.
OBJECTIVE:To study the association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women through Meta-analysis.
METHODS:The PubMed database, Embase database, CNKI database and Wanfang database were searched for the observational studies about the association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women. The standardized mean difference was considered as the effect indicator and calculated with random or fixed effect models according to the significance of heterogeneity.
RESULTS AND CONCLUSION:A total of 9 articles were included with a total of 8 799 and 9 365 participants for which vertebral and spine bone mineral density had been measured, respectively. The analysis results showed that the bone mineral density of the postmenopausal women with the G al ele of G1181C was significantly lower than that of the postmenopausal women with the C al ele. Similar results were obtained in Caucasian population subgroup analysis. The Caucasian population subgroup analysis showed that the bone mineral density of the postmenopausal women with GG genotype of G1181C was lower than that of the postmenopausal women with GC and CC genotype. The results indicate that there is association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women, and G al ele is a risk factor for lower bone mineral density.
6.Effect of nerve invasion on long term survival of middle and distal cholangiocarcinoma
Peng LI ; Hao GUO ; Lanbin LI ; Yan LIU
International Journal of Surgery 2017;44(1):23-26,封3
Objective To analyze the long-term survival of patients with middle and distal cholangiocarcinoma,and to explore the influence of nerve invasion on the survival time.Methods We retrospectively analyzed the clinical data of 161 patients with middle or distal cholangiocarcinoma in General Hospital of Fushun Mining Bureau between January 2012 and June 2016.All the patients were divided into nerve group and control group according to the nerve invasion.And then the perioperative and prognosis data of the two groups were analyzed and compared.Results In our research,all patients were successfully completed surgery,the incidence of neural invasion was 47.2% (76/161),more common of the nerve invasion in distal cholangiocarcinoma and phase Ⅱ cholangiocarcinoma.The perioperative mortality rate was 3.7% (6/161),and the incidence of complications was 27.3% (44/161).The 1 years,2 years and 3 years survival rates of middle and distal cholangiocarcinoma were 78.7%,42.1%,20.0%,the average survival time was(23.8 ±1.2) months.The 1 years,2 years and 3 years survival rates of nerve group and control group were 67.1%,29.8%,9.4% and 89.0%,52.6%,26.8%,the average survival time were (18.6 ± 1.4) months and (27.3 ± 1.6) months.The prognosis of nerve group was significantly poor than the control group (P < 0.05).Conclusions Nerve invasion is a common way of invasion and metastasis of cholangiocarcinoma.And it has a poor prognosis in cholangiocarcinoma patients with nerve invasion.
7.The correlation analysis of the heart rate variability and executive function in patients with insomnia
Yan WANG ; Xianju GUO ; Xinghua HAO ; Jinghua WANG ; Yaohui CUI
Chinese Journal of Behavioral Medicine and Brain Science 2014;23(8):684-686
Objective To investigate status about the heart rate variability and executive function in patients with insomnia.Methods The study involves 165 cases of patients with insomnia and 92 cases normal.The Pittsburgh Sleep Quality Index (PSQI) screening for insomnia,Wisconsin Card Sorting Test (WCST) for the assessment of executive function and Self-generate Physiological Coherence System (SPCS) were used to collect the data of heart rate variability (HRV).Results The SDNN,RMSSD,TP,LF,HF of HRV of the insomnia group were significantly higher than these factors of control group (t=2.712,2.726,2.427,2.542,2.338,P<0.05 or P< 0.01).In the WCST,number of completed categories,conceptualization percentage of the group of patients with insomnia were significantly lower than the control group (t=-2.368,-2.165,all P<0.05),persistent errors,persistent error percentages,continuous number of responses of patients' group were significantly higher than the control group (t=2.712,2.991,2.690,all P<0.01).TP,LF,HF,LF/HF of HRV and the number of error responses,persistent errors,persistent error percentages,continuous number of responses of WCST had significant negative correlations (r=-0.171--0.245,P<0.05 or P<0.01),while the conceptualization percentage were significantly positively correlated (r=0.198-0.227,P<0.05 or P<0.01).Conclusion The results show that insomnia not only cause individual autonomic disorders,but damage the executive function of prefrontal.HRV and executive function in patients with insomnia have close relationships.
8.Establishment of mice breast cancer model in BALB/c mice with 4T1 cells
Li YAN ; Li LI ; Fang HAO ; Wei SHEN ; Linlei ZHANG ; Hao GUO
Chinese Journal of Immunology 2014;(6):794-796
Objective:To establish a model of mice breast cancer of 4T1 cells in BALB/c mice and choose the best model making method.Methods:Ninety mice were divided into three groups randomly ,with 30 in each group injected by 4T1 cells suspension of 1×106 ml-1 ,1×107 ml-1 ,1×108 ml-1 respectively.Each group of mice were randomly divided into two groups which were inoculated on the chest wall and lateral abdominal wall respectively.Tumor formation time ,tumor growth rate and the 8 week survival rate in each group were compared ,and pathological character was observed by C-erbB-2 immunohistochemistry staining.Results:Tumor growth rate in cells suspension of 1×107 ml-1 was high and grow steadily.Tumor growth rate wasn′t correlative with 4T1 cells injection in different parts.The result of C-erbB-2 immunohistochemistry staining was positive.Conclusion: Injection of 4T1 cells suspension with 1 ×107 ml-1 is the best way to male tumor model in three suspension.
9.Preliminary investigation in critical care medicine contents and methods for standardized training residents
Qindong SHI ; Hao LI ; Lan GAO ; Qinyue GUO ; Litao GUO ; Jinqi YAN
Chinese Journal of Medical Education Research 2017;16(6):601-604
Resident standardization training is an important means of clinical physician training in our country. Critical care medicine has important status in the training process. It is the important link to ensure the quality of resident standardization training. Residents should grasp the identification and early detection of critical ill patients. Residents should also get the ability of general basic management for critical condition and the doctor-patient communication ability. In practice, we have explored the training mode of standardized training of resident doctors in critical care medicine by developing detailed training outline, a variety of teaching methods and emphasizing the cultivation of clinical work ability.
10.Discovery of topiramate's new functions based on medicinal property combinations and study on its mechanism.
Guang-Ji LI ; Hao GU ; Wei-Jia GUO ; Yan-Ling ZHANG ; Yun WANG ; Yan-Jiang QIAO
China Journal of Chinese Materia Medica 2014;39(13):2396-2399
OBJECTIVETo study topiramate's new functions according to the medicinal property combinations, in order to apply the traditional Chinese medicinal theory in discovering new purposes of old drugs.
METHODAccording to New Traditional Chinese Medicinal Families--Chemical Traditional Chinese Medicines, the authors found out topiramate's property. Then based on the therapeutic principle of diabetes, hypertension, epilepsy and lung cancer, as well as the relations of efficacies and medicinal property combinations, they summarized the corresponding medicinal property combination modes, compared topiramate's medicinal property combination mode with corresponding medicinal property combination modes of these diseases, and predict topiramate's new functions.
RESULTAccording to the comparison, the corresponding medicinal property combinations were consistent with topiramate's medicinal property combinations as evidenced by corresponding literatures, whereas other medicinal property combinations were not.
CONCLUSIONBased on medicinal property combination modes, the authors screened topiramate's new functions according to e of TCM clinical experience, discovered topiramate's therapeutic effects on diabetes, hypertension and lung cancer in addition to epilepsy, and explore new drug function according to medicinal property combination modes, which could help greatly shorten the new drug R&D period.
Diabetes Mellitus ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Fructose ; analogs & derivatives ; chemistry ; therapeutic use ; Humans ; Hypertension ; drug therapy ; Lung Neoplasms ; drug therapy ; Phytotherapy