Objective: To investigate the neuroendocrine properties of adrenalcortical tumors. Methods: We retrospectively reviewed the clinical data of 99 adrenalcortical tumor patients, who were treated in Changzheng Hospital form June 1999 to June 2005. Expression of neuron specific enolase (NSE), chromogranin A (CgA) and synaptophysin (Syn) proteins were examined by immunohistochemistry (S-P method) using monoclonal antibodies. The general data of patients, including the age, symptoms, laboratory findings, and pathological types, were collected and subjected to statistical analysis with SAS v6. 12 software. Results: The expression of all the above 3 proteins was found in adrenalcortical adenoma tissues, with the positive rate of NSE being 80%, the positive rate of CgA being 48.9%, and the positive rate of Syn being 75.6%; the positive rates in the adrenalcortical carcinoma tissues were 77.8%, 22.2%, and 77.8%, respectively; and those in the normal adrenal tissues was 20%, 0%, and 10%, respectively. The positive rates of 3 proteins in adrenalcortical tumors was significantly higher than those in the normal adrenal tissues (P

Objective To summarize the complications after cervico-occipital fusion and to explore the methods of the prevention and treatment of complications. Methods Three hungred and eighteen patients were fused in cervico-occipital region from January 1985 to January 2003. Of them, 65 cases with postoperative complications were reviewed. The complications in perioperative period comprised vertebral artery injury during the operation, temporary injury of nerve root, leakage of cerebrospinal fluid, spinal cord injury or deterioration, infection and asphyxiation. The complications in mid- to long-term included bone graft nonunion or delayed fusion, vertebral body shifting, internal fixation implants breaking or loosening, slipping of screws and pseudojoint formation. The complications in bone graft donor region were haematoma, pain and infection. Two physicians were assigned to follow-up the patients. Results Three hungred cases were followed up from 18 months to 18 years with an average of 6 years and 8 months. There were 15 cases with complications after surgery in single fusion group, the incidence was 24.4%. 1 case deteriorated of spinal function after surgery, 2 with bone graft displacement, 1 with bone graft absorption, 1 with bone graft nonunion who recovered after reconstruction. 50 complications presented in internal fixation group, the incidence was 19.1%, Foramen magnum was decompressed in 1 case with vertebral displacement and fixed with Cervifix. Odontoid process was resected in 1 case with atlanto-axis dislocation and deterioration of spinal function through oral approach and cervico-occipital region was fused, the symptom was improved obviously after operation. Spinous process fracture was found in 1 case 3 months later post-fixation with Simmons wire, the atlanto-odontoid vertebra was fused again and fixed with Vetex, the bone graft was fused completely. The temporary injury of nerve root due to wrong directions of the screws, were adjusted during the operation. 4 patients who felt pain caused by screw, which were then extruded out. 2 cases with vertebral artery injury were ligated or bleeding ceased after screw fixation. Conclusion The key points to reduce the complications are familiarity with dissection and operative skills. Appropriate internal fixation also can reduce the complications. Pressure dressing was useful to eliminate the haematoma in bone graft donor region.

[Objective]To summary the methods and results of free hand insertion with the lateral mass screw of atlas and vertebra dentata pedicle screw.[Method]Fifty samples of fresh adult atlas and dentate were used.There were 25 males and 25 females samlpes.Then rongeur forceps was used to bite the bone of entrance.The way of screw was made by Kirschner wire of 1.5mm and 2.5mm in diameter.Guide pin was inserted along the way.The screws were placed in lateral mass of atlas beside the posterior arch of atlas about 18～20mm and the point of intersection above 2mm inferior boarder of posterior arch.Vertical with coronal plane,the tip of the screw was made 5? to side of head in sagittal plane.The site of the screws placed in vertebra dentate was divided equally the inferior articular process of axis,made 15?with sagittal plane and 30?with cross section.There were 26 patients with Atlantoaxial vertebra unsteadiness,15 males and 11 females,with an average age of 39.5 years.Seventeen cases were used with screws and rod system and 9 patients were used screws and plate system.Preoperative JOA scores were from 5.1 to(10.6),with an average of 7.6.[Result]There were no cases with spinal cord and vertebral artery injury after surgery.Follow-up duration was from 10 to 25 months,with an average of 14 months.The postoperative JOA scores were from 12.9 to 16.5,with an average of 14.8.The improve rate was 87.6 percent.The plant bones all fused and there were no internal fixation rupture and mobiled.[Conclusion]The technique of free hand insertion with the lateral mass screw of atlas and vertebra dentata pedicle screw is simple,fixation is reliability and is well worth to spreading.

Objective To study the intracellular calcium ([Ca2+ ]i) in myocardial cell of obesity rats induced by high-fat diet. Methods Male Sprague-Dawley (SD) rats were divided into obesity resistant (OR, n=15), normal (Nor, n=15) and obesity prone (OP, n=15) group after fed with high-fat diet for 10 weeks. Their body fat and serum lipids were measured. Myocardial cells were isolated with Langendorff perfusion and [Ca2+]i was measured with calcium indicator Fluo-3/AM and laser scanning confocal microscope after KCl depolarization and caffeine- induced. Results Compared with those in Nor and OR rats, the epididymal fat, perirenal fat, omental fat and body fat increased in OP rats (P<0.05), as well as the the level of total cholesterol, triglyceride and low density lipoprotein (P<0.05); the vary of [Ca2+]i elevation and restoration were lower (P<0.05). Conclusion The vary of [Ca2+ ]i elevation decreases in OP rats after KCl depolarization and caffeine-induced, that may associated with arrhythmia in obesity rats.

This paper designs a heart sound processing analog circuit, in which the amplification circuit, the filter circuit and the lifting of electrical level circuit are involved. It also gives a safety design problem analysis using an electret capacitor microphone to pick-up the heart sound signals. It is proved that the circuit is reliable and effective.

Automation workstation refers to a kind of fully automatic platform,which use the automatic technology to analysis,processe batches of biological samples.It can finish liquid handling,transfering,mixing and other operatious in short time,particularly suitable for PCR templates'preparation of large amount of biological samples.This article mainly discusses the basic patterns,methods and forensic applications of automated DNA extraction workstation,aimed at making the technology more widely applied in forensic DNA analysis.

Data is the basis and soul of clinical trials. To obtain accurate data, strict and standard data management is essential, which can be effectively supported by quality control in statistical analysis. In this paper, we briefly introduce the concept of the quality control in clinical trials, and describe its contents and methods. We hope that this work will be helpful to the application of statistical quality control in data management of clinical trials.

Objective To investigate the compliance with medical therapy in elderly patients with benign prostatic hyperplasia (BPH).Methods The BPH patients aged 66 to 93 treated with 5α-reductase inhibitor (finasteride) and/or α-blockers (tamsulosin,doxazosin or terazosin) were followed up for 6 months.The retrospective data,including international prostatic symptom score (IPSS),quality of life (QOL),maximum flow rate (Qmax),prostate volume (PV),the information of medical types,total number of days of medication supplied,discontinuation and switching of therapy,and adverse effects were collected and analyzed.Results In all 103 subjects,30 patients (29.1％) received α-blockers,25 patients (24.3％) received finasteride,and 48 patients (46.6％)received combination therapy.After a follow-up of 6 months,the medication possession ratio (MPR)was 0.89±0.23 with a good-compliance rate (MPR≥0.8),discontinuation rate and switching rate were 79.6％ (82 cases),5.8％ (6 cases) and 6.8％ (7 cases),respectively,while the efficacy rate,the adverse reaction rate,incidence of acute urinary retention (AUR) and incidence of BPH related operations were 73.8％ (76 cases),12.6％ (13 cases),15.5％ (16 cases) and 4.9％ (5 cases),separately.The MPR (0.90±0.24) and good-compliance rate (84.0％) in patients treated with finasteride were both greater than those with α-blockers (0.87 ± 0.25,76.7％) and combination therapy (0.89 ±0.22,79.2％),but with no significant difference.Compared with patients with badcompliance,the efficacy ratio of medical therapy was significant higher in patients with goodcompliance (81.7％ vs.42.9％),while the incidence of adverse effects and AUR were significant fewer (7.3％ vs.33.3％ and 9.8％ vs.38.1％).Conclusions Elderly patients received either monotherapy or combination therapy with finasteride and α-blockers have good-compliance,which might be associated with the good effect of medical therapy and fewer adverse effects.

Objective To express functional haemegglutinin(HA)protein in two different bacularvirus expression systems.Methods The whole open reading frame of A/Sichuan/1/2009(H1N1)HA was obtained by synthesis,and the HA protein were expressed in insect cells by two different bacularvius expression systems:BaculoGold system and Bac-to-Bac system. Soluble HA protein was identified by Western blot and haemegglutination test. Results The correct full length of HA gene was obtained and cloned into pAcGP67B and pFAST Bacl vectors,respectively.After 3 rounds of virus amplifyjng by re-infection of Sf9 cells,the HA protein was detected in supematant of BaculoGold system and in intracellular of Bac-to-Bac system which is much better than the former.Purified HA protein was positive not only identified by Western blot,but also detected by haemegglutinin test. Conclusion Functional HA protein was successfully expressed in two distinct bacularvirus expression systems,of which the Bac-to-Bac bacularvirus expression system is more suitable for expression of A/Sichuan/1/2009(H1N1)HA protein.