Objective To investigate the necessity of dilution in samples with high concentrations of D-Dimer and optimum dilution multiple.Methods Quality control products and calibration were detected by using Sysmex CS5100 for precision evaluation,including within-batch and between-run precision.Calibration were detected for validation of linear range and clinical reportable.Samples with D-Dimer<5 mg/L and fibrinogen degradation products(FDP)<20 μg/mL were serially diluted and detected to calculate recovery rate.Samples with D-Dimer>5 mg/L and FDP>20 μg/mL were also serially diluted and detected to calculated recovery rate.Results Within-batch and between-run coefficients of variation were both less than 3%.Within the scope of 0.207-5.170 mg/L,the linear distribution was fine.The clinical reportable range was 0.207-165.440 mg/L.For samples with D-Dimer<5 mg/L and FDP<20 μg/mL,no antigen excess phenomenon was found,and gradient dilution was not necessary.For samples with D-Dimer>5 mg/L and FDP>20 μg/mL,there was obvious antigen excess phenomenon,and gradient dilution was required.Conclusion For samples with D-Dimer>5 mg/L and FDP>20 μg/mL,dilution should be performed to ensure the accuracy of detected results.

Adolescent ; Adult ; Aged ; Female ; Humans ; Immunophenotyping ; Leukocyte Common Antigens ; immunology ; Male ; Middle Aged ; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma ; immunology ; Young Adult

OBJECTIVETo study the relationship between Chinese medicine syndrome and serum level of high-sensitivity C-reactive protein (hs-CRP) in patients with stable coronary heart disease (CHD).

METHODSSerum hs-CRP level was determined in 346 patients with stable CHD, whose diagnoses were confirmed by coronary angiography, and the correlation between patients' syndrome and serum hs-CRP level was analyzed.

RESULTSIn all the CHD patients enrolled, the most commonly encountered Chinese medicine complex syndromes were the qi deficiency and blood-stasis syndrome, the yang deficiency and blood-stasis syndrome, and the qi deficiency with blood-stasis and turbid phlegm syndrome; the dominant syndrome types were blood-stasis, qi deficiency and phlegm-turbidity, which revealed in 324 patients (93.6%), 189 patients (54.6%) and 140 patients (40.5%), respectively. Comparisons of hs-CRP level between different complex syndromes and syndrome elements showed no significant difference (P > 0.05). However, for patients with phlegm-turbidity syndrome, the hs-CRP level was much higher in patients tended to heat than in those tended to cold (2.23 +/- 2.12 mg/L vs 1.59 +/- 1.27 mg/L, P < 0.05). Besides, the score of blood-stasis syndrome showed no correlation with hs-CRP level (Person correlation coefficient: 0.069, P = 0.203).

CONCLUSIONThe elevation of hs-CRP level is closely cor related with phlegm-heat syndrome in stable CHD patients. As an inflammatory biomarker, hs-CRP may be regarded as one of the microcosmic indices of toxin in Chinese medicine, which provides an objective evidence for the pathogenesis hinge of accumulated heat to transform toxin, so it is worthy of further study.

Adult ; Aged ; C-Reactive Protein ; metabolism ; Coronary Angiography ; Coronary Disease ; blood ; diagnosis ; Diagnosis, Differential ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged

Objective To compare high-frequency ultrasonography and MRI in diagnosis of medial patellofemoral ligament (MPFL) injury after acute lateral traumatic patellar dislocation(LTPD).Methods According to surgical data of 35 patients with acute LTPD,the incidence and sites of MPFL injury were analyzed.Further,based on high frequency ultrasonography and MRI data,the sensitivity,specificity and accuracy of high-frequency ultrasonography and MRI in different MPFL injuries were analyzed and compared.Results Among 35 patients,there were 21 cases with complete MPFL tear and 14 cases with partial MPFL tear.Tear of MPFL at the femoral attachment in 19 cases,at the patellar attachment in 15 cases,and at the mid-substance in 1 case.The sensitivity,specificity,accuracy of high-frequency ultrasonography diagnosis were 78.6％,95.2％,88.6％ respectively on partial MPFL tear and 95.2％,78.6 ％,88.6 ％ respectively on complete MPFL tear.The sensitivity,specificity,accuracy of MRI diagnosis were 85.7％,90.5％,88.6％ respectively on partial MPFL tear and 90.5％,85.7％,88.6％ respectively on complete MPFL tear.There were no significant difference between high-frequency ultrasonography and MRI in diagnosis of partial and complete MPFL injury (x2 =0.243,0.267; P =0.622,0.599).Conclusions MPFL formoral attachment is most vulnerable to damage,followed by patellar attachment.High-frequency ultrasonography,as well as MRI,can be used clearly to diagnose MPFL injury and accurately classify the damage,and it is also an easy,reliable,rapid method,which can be applied routinely to diagnose MPFL injury after actue LTPD.

BACKGROUND: Gastrodin (GAS) is widely used as adjuvant therapy for vertigo, headache and hypertension. However, it is recently noticed that GAS might be used as an agent for treating obesity.OBJECTIVE: To set up obese rats of high-fat diet to observe the effects of different concentrations of GAS on body mass and serum metabolite levels and to analyze its possible mechanism.DESIGN: A randomized and controlled animal experiment.SETTING: Institute of Physiology and Psychology, School of Basic Medical Science, Lanzhou University.MATERIALS: This study was performed at the Institute of Physiology and Psychology, School of Basic Medical Science, Lanzhou University and Gansu Provincial Key Laboratory of Pre-clinical Study for New Drugs from June to August in 2007. Forty-four healthy one-week-old male SD rats, weighing (99.57±2.13)g, were purchased from Shanghai SILAIKE Laboratory Animal Co., Ltd. Disposal of animals was in accordance with the animal ethics standards. Basic animal feed was provided by Suzhou Shuangshi Laboratory Animal Feed Science and Technology Co., Ltd. High-fat forage were self-made in the authors' laboratory. Each 100 gram of high-fat forage consisted of basic feed (57.5g), egg yolk powder (11.79g), lard (10g), pig bile salt (0.2g), casein (7g), milk power (13g), salt(0.085g), and yeast powder (0.425g), and the 100 gram of high-fat forage contained of fat (22.07g), protein (23.7g), carbohydrate (39g), and quantity of heat (472.16 calorie). GAS (98% in purity) was purchased from Shaanxi Xuhuang Botanical Science & Technology Development Co., Ltd. Malondialdehyde (MDA) and total antioxidative capability (T-AOC) kits were purchased from Jiancheng Bioengineering Institute, Nanjng, Jiangsu Province.MAIN OUTCOME MEASURES: The body mass was measured every seven days. The food intake in each group was monitored in every morning. At the end of the experiment, femoral artery blood samples were collected to determine the blood glucose, the serum levels of MDA, T-AOC, Insulin, free fatty acid (FFA), glutamate-pyruvate transaminase (GPT), glutamic oxalacetic transaminase (GOT) and blood lipid profile. Insulin resistance index (IRI) and insulin sensitivity index (ISI) were calculated as IRI=(FBG×FINS)/22.5 and ISI=1/(FINS×FBG).RESULTS: All 44 rats were included in the final analysis. Body mass: The body mass in the HFFC group was significantly higher than in the NC group from 4th-8th weeks (P＜0.01), while the body mass in GAS groups was lower compared to HFFC group (P＜0.05-0.01). There were no significant differences among the GAS-H, GAS-M, and GAS-L groups (P＞0.05). Therefore, GAS had no dose-dependent relationship in inhibiting the body mass of obese rats of high-fat diet. Caloric intake: The caloric intake was significantly higher in the HFFC group than in the NC group (P＜0.01), and was significantly decreased in GAS group compared to NFFC group from the 4th week (P＜0.05-0.01). Serum levels of MDA, T-AOC, GPT and GOT: The serum level of T-AOC was decreased and that of MDA, GPT were increased significantly in the HFFC group compared with NC group (P＜0.01, P＜0.05). In the GAS-L group, T-AOC, level was significantly increased and MDA level was significantly decreased compared to HFFC group (both P＜0.01). Levels of blood glucose and insulin: In the HFFC group, blood glucose level and IRI were significantly increased, and ISI was obviously decreased compared to NC group (P＜0.05-0.01). In the GAS-L group, blood glucose level and IRI were significantly decreased, and ISI was significantly increased compared to HFFC group (P＜0.05-0.01). FFA and lipoprotein cholesterol levels: In the HFFC group, FFA and low-density lipoprotein cholesterol levels were increased and high-density lipoprotein cholesterol level was decreased compared to NC group (P＜0.05-0.01).CONCLUSION: GAS may play an important role in inhibiting rats' body mass of high-fat diet. The mechanism of action may be related to GAS regulating the metabolism of blood glucose and FFA, improving IRI and elevating T-AOC.

Objective To explore the influence of tranSCription factor FOXC2 in angiogenesis of breast cancer MCF-7 cells and to clarify the action mechanism of FOXC2 in promoting tumor angiogenesis.Methods FOXC2 gene and empty vector gene were transfected into breast cancer of MCF-7 cell line with FOXC2 lentivirus gene transfection technique to obtain stable transfection cell line. The MCF-7 cells were devided into non-transfected group,empty-vector group and over-expression group.Matrigel assay and Transwell chamber test were used to observe the changes of tube formation and migration ability of human umbilical vein endothelial cells (HUVECs)in MCF-7 cells supernatant in various groups. PT-PCR and Western blotting methods were applied to detect the expressions of FOXC2,DLL4 and Notch1 mRNA and protein.Results Compared with non-tranfected group and empty-vector group,the tube formation and the migration number of HUVECs in FOXC2 over-expression group were increased(P<0.05);the expressions of FOXC2,DLL4 and Notch1 mRNA and proteins were significantly increased(P<0.05).Conclusion The FOXC2 over-expression in MCF-7 cells can increase the tube formation ability and migration ability of HUVECs,and its mechanism may be related to Notch signaling pathway.

OBJECTIVETo explore the clinical manifestations of "blood-stasis and toxin" (BST) in patients with stable coronary heart disease (CHD) on the basis of "BST Causing Catastrophe" hypothesis, by analyzing the occurrence of acute cardiovascular events (ACEs) in a one-year follow-up period.

METHODSTwo hundred and fifty-four CHD patients in stable stage, whose diagnosis confirmed by coronary arteriography, were followed-up for one year with their clinical data recorded detailedly. The relationship between the occurrence of ACEs and personal history, body constitution, past history of illness, family history, clinical symptoms, physical signs, syndrome types and laboratory indexes were analyzed by single-variate and multivariate logistic stepwise regression method.

RESULTSDuring the follow-up period, two cases out of the 254 patients were lost, no case of death or acute myocardial infarction was seen, three cases underwent percutaneous coronary intervention, and 25 cases were hospitalized for unstable angina. Multivariate logistic regression analysis showed that hesitant or intermittent pulse, retrosternal pain, usual pharyngodynia and headache were the most important influencing factors for occurrence of ACEs (P < 0.05). High-sensitivity C-reactive protein (hs-CRP) > or =3 mg/L also indicated a predictive trend for ACEs occurrence, although logistic analysis showed no statistics difference (P = 0.094).

CONCLUSIONRetrosternal pain, headache, hesitant or intermittent pulse, usual pharyngodynia and increased hs-CRP might be taken as the clinical manifestations of BST, which provides a basis for applying Chinese medicine intervention (activating blood circulation and detoxifying) on high-risk CHD patients. Further demonstration is needed.

Adult ; Aged ; C-Reactive Protein ; metabolism ; Coronary Artery Disease ; diagnosis ; metabolism ; pathology ; Female ; Humans ; Inflammation ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged

Objective To study the prognosis and pre-procedural independent risk factors for pa-tients with no-reflow (NR) phenomenon during percutaneous coronary intervention (PCI). Methods Pa-tients with or without NR phenomenon during PCI procedures from January 2000 to January 2005 were studied retrospectively. The clinical data preoperative and the incidence of major adverse cardiovascular events (MACE) between the two groups were compared. Univariate analysis and multivariate Logistic analysis were used to select the risk factors for NR phenomenon. Retrospectively was reviewed for (35.8 ± 15.3)months. Results The NR group had more significant incidence of MACE. Multivariate Logistic analysis showed that the predictive factors for NR were (1) Smoke index ≥ 300(OR = 2.81,95%CI: 1.61-4.38 ,P =0.007). (2) Fasting blood glucose level before PCI ≥ 11.1 mmol/L (OR = 3.39,95%CI: 1.51-4.89,P = 0.000 ). (3) Absence of angina pectoris attack within one month before PCI (OR = 2.39,95%CI: 1.22-3.78,P = 0.009). Conclusions The prognosis is poor for the PCI patients with NR phenomenon. Those patients whose fasting blood glucose level before PCI ≥ 11.1 mm01/L, smoke index ≥ 300 and absence of angina pec-toffs attack within one month before PCI have higher incidence of NR phenomenon.

Objective To observe the morphologic changes of peripheral blood lupus cell in patients with systemic lupus erythematosus and investigate its relationship with disease activity in systemic lupus ery- thematosus.Methods Modified classical blood clotting method to observe the morphological changes of pe- ripheral blood lupus cells in 80 cases with systemic lupus erythematosus.Fifty cases were in active stage,and 30 cases were in stable stage.Comparison of serum autoantibody,complement and SLEDAI were also carried out.Results There was significant association between lupus cells in special morphous and autoantibody, such as anti-double-stranded DNA antibody,anti-nucleosome antibodies,complement C3、C4 and SLEDAI(r= 0.588,P=0.056:r=0.759,P=0.135;r=-0.648,P=0.058;r=-0.589,P=0.057,r=0.686,P

OBJECTIVETo explore the tongue manifestations for the blood-stasis and toxin syndrome in the stable patients of coronary heart disease (CHD) according to the acute cardiovascular events (ACEs) in one-year follow-up which based on the pathogenesis hypothesis of "blood-stasis and toxin causing catastrophe".

METHODSTotally 254 stable CHD cases were enrolled after diagnosed by coronary angiography, their tongue appearances were recorded by the digital camera of uniform type, 29 cases with ACEs during one-year follow-up were assigned in ACEs group. The non-ACEs were matched in proportion of 2:1 according to the gender, age (±2.5 years), diabetes mellitus history and previous acute coronary syndrome hospitalization history in the non-ACEs group, and 54 cases were eligibly included. The differences of tongue appearance between the ACEs and non-ACEs group were compared.

RESULTSFifteen cases manifested with bluish tongue (including bluish-grounding or bluish purple tongue), among which 11 cases (37.9%) in the ACEs group and four cases (7.4%) in the non-ACEs group, and there was significant difference (P<0.002). Twenty six cases showed yellow tongue coating in the non-ACEs group, which was significantly higher than that in the ACEs group (48.1 vs. 10.3%, P=0.001). The tongue of sticky greasy coating was more frequently occurred in the non-ACEs group than that in the ACEs group (66.7% vs. 41.4%, P=0.026). The proportion of purplish-red sublingual vessel was higher in the ACEs group than that in the non-ACEs group (41.4% vs. 20.4%, P=0.041). Odd ratio (OR) analysis showed that the patients with bluish tongue, purplish-red sublingual vessel, dry-greasy or dirty greasy coating were more likely to experience ACEs during one-year follow-up (OR: 11.67, 95%CI: 3.34 year 3.34-40.81, P<0.001; OR: 2.76, 95%CI: 1.02 1.02-7.44, P<0.05; OR: 3.12, 95%, CI: 0.89 0.89-10.92, P=0.066).

CONCLUSIONSThe bluish tongue (including bluish-grounding or bluish purple tongue) and purplish-red sublingual vessel were potential tongue manifestations of blood-stasis and toxin. The tongue coating changing from sticky greasy to dry greasy or dirty greasy was also probably a tongue manifestation of "transforming toxin", which need demonstration by further study.

Adult ; Aged ; Case-Control Studies ; Coronary Disease ; blood ; pathology ; Female ; Humans ; Male ; Middle Aged ; Risk Factors ; Syndrome ; Tongue ; pathology