Leukemia is a kind of cloning disease from malignant hematopoietic stem cells. Its patho-genesis may have relationship with proliferation out of control,dysdiffreentiation and inhibition of apoptosis about leukemia cells. The study confirms that CD44 is expressed in leukemia cells,promoting the occurrence and development of leukemia through participation in cell adhension,proliferation,migration and invasion behavior. In vitro experiment shows that CD44 targeted therapies can effectively kill leukemia cells,and reduce the toxicity to normal tissue cells. Therefore,aiming at the design of the anticancer drugs targeted for CD44 is expected to be used in clinical treatment of leukemia.

Objective To investigate the gene expression of sodium pump α-subunit in aortic smooth muscle of 1-kidney-1-clip (1k1c) hypertensive rats. 　Methods 1k1c hypertensive rats were prepared by partially ligating the left renal artery and removing the right kidney. 4 weeks later, all the rats were killed and sodium pump α1- , α2-, and α3-subunit in aortic smooth muscles were detected with reverse transcription p ol ymerase chain reaction (RT-PCR) method and immunohistochemical assay at both mR NA and protein levels, respectively. 　Resulsts Changes in the expression of sodi um pump α-subunit gene were found in aortic smooth muscles of 1k1c hypertensiv e rats: α1-su bunit increased at both mRNA protein levels, while α2- and α3-subunits r emained without changes. 　Conclusions There were great changes in the gene expression of so dium pump α-subunit in aor tic smooth muscles of 1k1c hypertensive rats, which might be related to the development of hypertension in this hypertensive model.

ObjectiveTo explore the distribution of sodium pump α-subunit isoforms at both mRNA and protein level in normal Sprague-Dawley(SD) rats. MethodsWith RT-PCR (reverse transcription-polymerase chain reaction) and immunohistochemical assay, sodium pump α1-, α2-, andα3-subunit isoforms were detected at mRNA level and protein level in the myocardium, liver, kidney, adrenal gland, aortic smooth muscle, pituitary and hypothalarnus in normal rats. ResultsSodium pump α1-, α2-, and α3-subunit isoforms distribute in a tissue-specific fashion in normal SD rats. α1 and α2 are the main isoforms and α3 is much less in myocardium. α1 is more than α2 or α3 isoform at mRNA level in liver, while all of α1-,α2-, and α3-isoform are less expressed at protein level. α1-isoform is abundantly but α2 and α3 are less expressed both at mRNA and protein levels in kidney. α2isoform is just more than α1 and α3 isoforms in aortic smooth muscle. All of three isoforms are expressed abundantly in pituitary. α2 and α3 are more and α1 is less expressed in hypothalamus. ConclusionThe results of this study have revealed the distribution of sodium pump α-subunit isoforms at both mRNA and protein level in normal SD rats, which might be helpful for the further studying the physiologic and pathologic roles of sodium pump.

Objective　To compare the effects of ouabain and digoxin on both the systolic blood pressure (SBP) and sodium pump α-subunit expression in myocardium of rats. Methods　Normal Sprague-Dawley (SD) rats were injected with ouabain, digoxin or normal saline (NS) everyday and indirect SBP was recorded once a week. Six weeks later, all the rats were killed and sodium pump α1-, α2-, and α3-subunit were detected in the left ventricular myocardium with RT-PCR method and immunohistochemical assay at mRNA and protein levels. Results　At the end of six weeks SBP of rats infused with ouabain increased significantly (132.6±9.0 vs 115.7±8.2 mm Hg， P＜0.01), while no difference of SBP was found between digoxin group and NS group. The effects of ouabain and digoxin on sodium pump α-subunit isoform expression in myocardium were also different: both ouabain and digoxin stimulated expression of α3-isoform whereas α2-isoform unchanged at both mRNA and protein levels. α1-isoform was decreased in ouabain group and α1-isoform unchanged in digoxin group at both mRNA and protein levels. Conclusion　It is suggested that both ouabain and digoxin could regulate sodium pump α-subunit isoform expression, which might be related to the physiological roles of endogenous ouabain and might be responsible for the difference between the pharmacological and toxicological effects of ouabain and digoxin, including their effects on blood pressure.

[Objective]To evaluate the clinical results of the patients with severe kyphosis and paraplegia in differnt spine segment due to tuberculosis of thoracic and lumbar which had been treated by different surgical procedure. [Methods]There were a total of 23 patients, 16 male and 7 female in this stuady. The average age were 25 years, ranging from 12~56 years old. Tuberculosis lesion were located in different spine segment(T1~4)four cases, (T5~10)four cases, (T11~L2) nine cases,(L3~5) six cases, two vertebral bodies were involved in 8 patients and three vertebralbodies involved in 12 patients. Surgical procedure:four patients treated through modified anterior cervico-thoracic approach.Five patients treated through one stageposterior total vertebral osteotomy, other patients combined with anterior-posterior approach, according to the patient's surgical situation, twelve patients accepted anterior and posterior combined operation, two patients were treated by two stages.The period of bone grafting fusion, kyphosis deformity correction degree ,and neurological function recovery record were investigated postoperatively.[Results]All patients got solid fusion in a average of seven months. The kyphosis deformity was corrected 49? in average, which was maintained well within follow-up period. Patients neurological function deficiency achieved completely recovery, the longest time being within 10 months. All patients were cured thoroughly and there was no seroius complication.[Conclusion]Different surgical procedure should be selected, according to different spine segments due to tuberculosis of thoracic and lumbar vertebrae with the extent of severe kyphosis and paraplegia, it can achieve thoroughly debridement of tubereulosis, solid interbody fusion, good correction of deformity and forever stability of spine with anterior or posterior internal fixation.It could get successful rehablitation for patient’s neurological function and improve the patient’s life quality.

Objective To compare the effects of ouabain and digoxin on both the systolic blood pressure and sodium pump α-subunit isoforms gene expression in the aortic smooth muscle of rats. Methods Normal SpragueDawley rats were injected with ouabain (20μg·kg-1 ·d-1 ,i. p),digoxin (32 μg·kg-1 ·d-1,i. p)and normal saline once a day, respectively, and indirect systolic blood pressure was recorded once a week. Six weeks later,all the rats were killed and sodium pump α1-,α2-,and α3-subunit mRNA levels were detected in the aortic smooth muscle with reverse transcription polymerase chain reaction(RT-PCR) method. Results The systolic blood pressure of rats infused with ouabain increased significantly at the end of week 6 [132. 6± 9. 0 mmHg (1 mmHg ＝ 0. 133 kPa)vs 115. 7±8.2mmHg, P ＜0. 01] ,while no difference of blood pressure was found between digoxin group and NS group (P＞0.05).The expression of sodium pump α-subunit isoforms in aortic smooth muscle was regulated by either ouabain or digoxin:both ouabain and digoxin increased α1- and α3-subunit expression, α2-subunit decreased in digoxin group but unchanged in ouabain group. Conclusion These results suggest that both ouabain and digoxin could regulate sodium pump α-subunit isoform expression, which might be related to the physiological roles of endogenous ouabain and might be responsible for the difference between the pharmacological and toxicological effects of ouabain and digoxin,including their effects on blood pressure.

Objective To explore the relationship between endogenous ouabain and the pathogenesis of hyper- tension. Methods ① Sixteen Sprague-Dawley (SD) rats were selected and randomly divided into two groups, and then the rats were injected with ouabain in dosage of 20μg/kg. d and normal saline (NS), respectively. The indirect systolic blood pressure of all the rats were recorded once a week. ②) Twenty-five lk1c (one kidney and one clipped) hypertensive rats were established and injected randomly with anti-ouabain antibody, normal rabbit IgG and normal saline, respectively. The direct blood pressure of all the lklc hypertensive rats were recorded by cannulated carotid artery for 3h after administration. Results The systolic blood pressure of rats injected with ouabain began to increase 2 weeks after ouabain administration and increased significantly at 6 weeks compared with NS group (17.7±1.2)kPa vs .(15.4±1.1)kPa, P＜ 0. 01). Anti-ouabain antibody could significantly decrease the blood pressure of 1 k1c rats, while the normal rabbit IgG could not. ConclusionThe results of this study indicate that endogenous ouabain might be one of the pathogenetic factors of hypertension.

AIM: To explore the role of endogenous ouabain (EO) in the development of hypertension and its secretion character in 1k1c hypertensive rats(HR). METHODS: EO contents of plasma and tissues in 1k1c HR were detected by ELISA. The relationships between plasma and tissues ouabain and blood pressure were analyzed. RESULTS: EO contents of plasma, heart, kidney, adrenal gland, pituitary and hypothalamus in lklc HR were significantly higher than those of normal rats, especially in the adrenal gland and hypothalamus. EO contents of serum, kidney and hypothalamus were correlated with blood pressure. CONCLUSIONS: EO might play an important role in the development of hypertension in 1k1c hypertensive rats. Adrenal gland might be the major source of EO.

Objective To study the expression of telomerase and its possible clinical significance in patients with condyloma acuminatum,bowenoid papulosis,Bowen's disease and vulvar squamous cell car- cinoma.Methods The human telomerase reverse transcriptase (hTERT) was detected by immunohisto- chemistry technique in all patients.Histopathological analysis was done in patients with bowenoid papulosis or Bowen's disease.Results There was a significant difference in the expression of hTERT among normal tissue,condyloma acuminatum and squamous carcinoma,and the expression rate increased successively from normal control to squamous carcinoma.In bowenoid papulosis,the expression of bTERT was significantly higher as compared to that in condyloma acuminatum,significantly lower as compared to that in squamous carcinoma,and was as the same as that in Bowen's disease.Histopathologically,the degree of cell atypia was significantly higher in Bowen's disease than that in bowenoid papulosis.Conclusions The expression of telomerase increases successively from normal skin,benign tumors to malignant carcinomas,which sug- gests that the expression of telomerase may play an important role in both cell proliferation and immortaliza- tion.

Objective To explore the reason for remedy radical operation after endoscopic submucosal dissection (ESD) for early gastric cancer.Methods The clinical data of 26 early gastric cancer cases who received remedy radical gastrectomy after ESD between January 2012 to March 2015 in Nanjing Drum Tower Hospital were analyzed retrospectively.Results All cases were followed up and mid follow-up was 25 months.There was no death nor tumor reccurence cases during the follow-up.Three cases suffered from complications in radical surgery for gastric cancer including afferent jejunal loop obstruction,adhesive intestinal obstruction,stomach paralysis respectively,all were cured by conservative therapy.Postoperative pathology found lymph node metastasis in 3 cases.Conclusions En bloc resection and negative resection margin,as well as free lymphatic metastasis are two important factors determing the survival of early gastric cancer patients undergoing ESD treatment.