2.Effect of rosiglitazone on SREBP-1 and TGF-β1 expressions and accumulation of ECM in renal tubular cells of Wistar rats treated with high fat diet
Jun HAO ; Yanping CAO ; Lin ZHU ; Shuxia LIU ; Huijun DUAN
Chinese Journal of Pathophysiology 2009;25(12):2430-2435
AIM: To study the effect of high fat diet on the expression of sterol regulatory element biding protein-1 (SREBP-1) and transforming growth factor β_1 (TGF-β_1) in renal tubular cells and rosiglitazone intervention. METHODS: Wistar rats were treated with high fat diet and rosiglitazone for 3 months. The serum glucose, serum insulin and serum triglyceride were detected. Oil Red O staining was used to observe the renal lipid deposit and Masson staining was for the detection of ECM accumulation. SREBP-1, TGF-β_1 and FN protein were determined by the methods of immunohistochemistry and Western blotting. SREBP-1 mRNA was detected by in situ hybridization. RESULTS: Rosiglitazone prevented effectively the increase in serum glucose, serum insulin and serum triglyceride resulted from high fat diet. High fat diet led to lipid droplet formation in renal tubular cells and interstitial ECM accumulation, which was decreased by rosiglitazone treatment. Compared to normal rats, SREBP-1 protein and SREBP-1 mRNA showed high expressions in high fat diet rats that were lowered by rosiglitazone. The precursor segment and mature segment of SREBP-1 protein were decreased by 27.39% and 27.32%. Similarly, the high expressions of TGF-β_1 and FN protein in kidney of high fat diet rats were also prevented by rosiglitazone intervention. Compared to high fat diet rats, the expression of TGF-β_1 in rosiglitazone treatment rats was lowered by 19.14%. CONCLUSION: Rosiglitazone prevents effectively the over-expression of SREBP-1 and TGF-β_1 in renal tubular cells, and decreases lipid accumulation and ECM production in rats fed with high fat diet.
3.Expression of SREBP-1 in kidney of type 1 diabetic rats and insulin intervention
Jun HAO ; Lin ZHU ; Zanhua RONG ; Huijun DUAN
Chinese Pharmacological Bulletin 1987;0(01):-
Aim To investigate the expression of SREBP-1(sterol regulatory element binding protein-1) in the kidney of type 1 diabetic rats and the effect of insulin.Methods The type 1 diabetic models were induced by high dose of STZ and rats were randomly divided into three groups: normal control group,diabetes control group and insulin treated group.At the 2nd week end,the triglyceride(TG) content in the kidney of experimental rats was measured by the assay kit and oil Red O staining.Furthermore,the expression of SREBP-1 protein was detected by the methods of Western blot and immunohistochemistry.The analysis of SREBP-1 mRNA was performed by in situ hybridization.Results Compared with the control group,the type 1 diabetic rats' renal triglyceride content markedly increased,and the result of Oil Red O showed that lipid deposited in the renal tubular epithelium.Triglyceride content markedly decreased after insulin treatment.The difference had statistic meaning,compared with the diabetes model group.Immunohistochemistry presented the results that SREBP-1 protein was up-regulated in renal tubular epithelium of diabetic rats and insulin treatment suppressed the increasing.The results of western blot showed that the precursor and mature segments of SREBP-1 protein in kidney of diabetes group rats were about 1.86 times and 1.77 times respectively of that of normal control group rats.In situ hybridization confirmed the increasing of SREBP-1 mRNA in renal tubular epithelium in diabetic rats.The effect of insulin treatment on SREBP-1 expression was detected by the methods of Western blot and in situ hybridization and it was found that the SREBP-1 mRNA and protein of kidney were down-regulated.Compared with the normal group,the difference has statistic meaning(P
4.The value of procalcitonin in the diagnosis and prognosis of stoke-associated pneumonia
Hao ZHU ; Jijun XU ; Jun HU ; Yingshuo WANG
Chongqing Medicine 2013;(31):3738-3739,3743
Objective To observe the value of procalcitonin (PCT ) in the diagnosis ,treatment and prognosis judgement of stoke-associated pneumonia(SAP) .Methods The serum procalcitonin ,hs-CRP and clinical pulmonary infection scores (CPSI) of two groups were detected :SAP group(including 27 patients of stoke-associated pneumonia) and control group(including 31 healthy ob-jects) .Results The CPSI ,hs-CRP and serum concentration of PCT in SAP group were significantly higher than control group (P<0 .01) ,and decreased significantly when get better after treatment .The serum concentration of PCT in SAP group was significantly higher than control group(P<0 .01) .In SAP group ,the serum concentration of PTC was positively associated with CPSI scorers . Moreover ,the results of ROC analysis showed that the cut-off of serum procalcitonin for the diagnosis of SAP was 0 .9 ng/mL .The sensitivety was 88 .6% and the specifity was 79 .3% ,which was superior to hs-CRP .Conclusion The serum concentration of PCT in SAP patients is higher and it is associated with the prognosis .It might be a better clinical marker for the diagnosis and prognosis of SAP .
5.Effect of increased serum TNF-αon lipid accumulation in kidney of diabetes
Song ZHANG ; Lin ZHU ; Jun HAO ; Shushen ZHENG
Chinese Pharmacological Bulletin 2014;(8):1161-1164,1165
Aim Todeterminetherelationshipbe-tween serum TNF-α and renal abnormal lipid metabo-lismindiabeticmice.Methods CD1micewerein-jected intraperitoneally with STZ (150 mg·kg-1 ) and the type 1 diabetic mice were determined with high fasting blood glucose ( >16. 7 mmol · L-1 ) 72 hours after injection. After fed for one month, normal control mice and diabetic mice were sacrificed. The serum TNF-α level was detected by the method of ELISA. Immunohistochemistry and Western blot were used to explore the expression of SREBP-1 and ADRP. Re-sults TheresultsofELISAshowedthatserumTNF-αwas increased by 7 . 73 times in diabetic mice compared with normal mice. SREBP-1 and ADRP expressions were located in renal tubular cells. Again, it was con-firmed by Western blot that SREBP-1 precursor seg-ment, SREBP-1 mature segment and ADRP were re-spectively enhanced by 2. 31 times, 1. 74 times and 1. 72 times in diabetic mice in comparison with normal control mice . In addition , the data analysis revealed a
positive correlation ( correlation coefficient 0. 914 ) be-tween serum TNF-αand renal ADRP expression. Con-clusion TheincreasedserumTNF-αmaybeoneof factors involved in renal lipid accumulation of diabe-tes.
6.The effects of gossypol acetate on the morphological features and the gene expression in the bone tissues of the femoral head of Sprague-Dawley rat in vivo after treated with dexamethasone
Hao LIN ; Jun LIU ; Bo WEI ; Rong ZENG ; Peiyong WANG ; Hao XIANG ; Weixiong GUO ; Zhaobo ZHU
Chongqing Medicine 2014;(22):2893-2896
Objective To explore the effects of gossypol acetate on the morphological features and the gene expression in the femoral head of Sprague-Dawley rat in vivo after treated with dexamethasone .Methods Dexamethasone(Dex) was injected into the abdominal cavity of SD rats at an dose of 10 mg/kg ,twice a week ,and feed gossypol acetate 5 mg · kg -1 · d-1 .The controls re-ceived saline 2 mL injection .The treatment lasted for 12 and 20 weeks .The slices of the femoral head were made for HE and immu-nohistochemical study .The total mRNA was extracted for RT-PCR assessment .Results The cancellous bone trabecular became sparse ,trabecular bone area ratio decreased ,bone marrow fat tissue increased .These changes were fitted for pathological character of bone necrosis .The gossypol acetate could not affect the pathological changes .The proportion of the positive stained osteoblasts increased ,adipocytes decreased .PPARγ,C/EBPα,11β-HSD1 expression enhanced ,Runx2 down regulated in the treatment groups and GAA group .Conclusion Dex can induce evident pathological changes conform to the characters of femoral head necrosis .They may have closed correlation between 11β-HSD1 and the gene expression .But GAA could not affected the pathological changes and abnormality of the gene expression .
7.Expression and significance of IL-10 and TGF-β1 in serum of patients with systemic lupus erythematosus
Qiang ZHU ; Minghua ZHU ; Jun LUO ; Hao WANG ; Jingzhi HE ; Hua LIU ; Xuyan WANG
Military Medical Sciences 2015;(4):276-278
Objective To investigate the serum levels of IL-10 and TGF-β1 and their significance for patients with sys-temic lupus erythematosus( SLE) .Methods A total of 135 cases were available for the study including 63 cases in primary group(A), 40 cases in remission group(B) and 32 cases in healthy control group(C).ELISA method was used to deter-mine the concentrations of IL-10 and TGF-β1 in serum.Finally, the results of group A were analyzed on the basis of System-ic Lupus Erythematosus Disease Activity Index( SLEDAI) .Results The level of IL-10 in serum of group A was significant-ly higher than that of group B and group C.The level of IL-10 in serum of group B was significantly higher than that of group C(P<0.05).The level of IL-10 in group A had active correlation with SLEDAI.The level of TGF-β1 in serum of group A was significantly lower than that of group B and group C.There was no statistical difference between group B and group C(P>0.05).The level of TGF-β1 in serum of group A had negative correlation with SLEDAI.The cases in group A were divided into three subgroups by SLEDAI:21 cases in mild activities subgroup, 30 cases in moderate activities sub-group,and 12 cases in severe activities subgroup, the levels of IL-10 and TGF-β1 in the three subgroups were significantly different(P<0.05).Conclusions IL-10 and TGF-β1 are involved in activity and severity of SLE.
8.Detection of Foxp3+ CD4+ CD25+ regulatory T cells in the peripheral blood of patients with condyloma acuminatum and its significance
Yan XU ; Jun YE ; Kejian ZHU ; Hao CHENG ; Ning ZHU ; Xianzhen CHEN
Chinese Journal of Infectious Diseases 2009;27(8):484-486
Objective To observe the levels of Foxp3+ CD+ CD25+ regulatory T cells in the peripheral blood of condyloma acuminatum (CA) patients and investigate their roles in the pathogenesis of CA. Methods The peripheral blood was collected from 30 CA patients (including 15 with relapsing and 15 with first onset) and 20 healthy controls. Peripheral blood mononuclear ceils (PBMC) were isolated and stained with anti-human CD4-PE-Cy5 and anti-human CD25-fluorescein isothiocyanate (FITC) monoclonal antibodies on cell membrane, followed by intraeellular staining with anti-human Foxp3-PE. The percentage of Foxp3+ CD4+-CD25+ regulatory T cells was detected by three-color flow cytometry. Comparison between groups was done by ANOVA test. Results The percentages of Foxp3+ CD4+ CD25+ regulatory T cells among total CD4 + T cells in CA patients and relapsing CA group were (3.4 ± 1.0) % and (4.7 ±+ 1.2) %, respectively, which were both significantly higher than that in healthy control group [(1.2±0. 5)%, P<0.01]. Furthermore, that in first onset CA group was (2. 1 ± 1.0) %, which was higher than that in healthy control group, but without statistical significance; but that in relapsing CA group was significantly higher than that in first onset group (P<0.05). Conclusions The number of Foxp3+ CD4+ CD25+ regulatory T cells increases in the peripheral blood of CA patients. The disorder of cellular immunity may be involved in the immunopathogenesis of CA.
9.The effects of DR2 on myocardial ischemic postconditioning and its underlying mechanisms.
Hong-Zhu LI ; Jun GAO ; Xiao-Min HAO ; Li-Min ZHANG ; Jun-Ting CHEN
Chinese Journal of Applied Physiology 2014;30(4):301-305
OBJECTIVETo study the effects of dopamin receptors-2 (DR2) on myocardial ischemic postconditioning and explore its underlying mechanisms.
METHODSThe myocardial ischemic postconditioning (PC) model was established in cultured primary rat neonatal cardiomyocytes which were then randomly assigned in the following groups: Nomial control group, Isehemia/reperfusion (L'R) group, PC (ischemic postconditioning) group, PC + Bro (Bromocriptine, a DB2 antagonist) group, PC + Hal (Haloperidol, a DB2 repressor) and PC + Hal + Bro groups. The lactate dehydrogenase (LDH) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in cell medium were analyzed by colorunetry. The cell ultrastructure changes were observed by transmission electron microscope. The cell apoptosis was analyzed using flowcytometiy. The protein expression level of D112 and activity of p-p38 and p-JNK were detected by Western blot.
RESULTSCompared with the nonnal control group, hR increased the protein expression level of DB2, enhanced LDH activity and MDA content, promoted cell injury and apoptosis, decreased SOD activity, up-regulated the activity of p-p38 and p-JNK. Compared with the hR group, although PC further increased the expression of DR2 protein, it decreased LDH activity and MDA content, cell injury and apoptosis, increased SOD activity, down-regulated activity of p-p38 and p-JNK. Bromocriptine treatment further enhanced PC-induced canlioprotective effect, yet Hal addition attenuated this enhancing effect exerted by bromocriptine.
CONCLUSIONThe activation of DB2 is involved in the protective effect of ischemic postconditioning on myocardial ischemia/reperfusion injury through down-regulating the activity of p-p38 and p-JNK.
Animals ; Apoptosis ; Cells, Cultured ; Ischemic Postconditioning ; JNK Mitogen-Activated Protein Kinases ; metabolism ; Myocardial Reperfusion Injury ; prevention & control ; Myocytes, Cardiac ; pathology ; Rats ; Rats, Wistar ; Receptors, Dopamine D2 ; physiology ; p38 Mitogen-Activated Protein Kinases ; metabolism
10.Voice Fatigue test in Primary School Teachers in Chengdu and the Potential Impacts on the Voice Health Care
Dan LV ; Hui YANG ; Yanan XU ; Jiao ZHU ; Jian ZOU ; Jun LIU ; Yitao ZHENG ; Hao XIAO
Journal of Audiology and Speech Pathology 2016;24(5):465-468
Objective To investigate the prevalence of voice disorder and voice fatigue mental state in the pri-mary school teachers in a district of Chengdu.Methods A random sampling survey included 389 teachers from a dis-trict of Chengdu after they filled out the throat symptoms questionnaire,carried out voice fatigue tests,and strobo-scopic laryngoscopy.Results The most common voice of discomfort symptoms was hoarseness,followed by sore throat,dry throat and vocal fatigue.There were 189 teachers,48.5%,with voice disorders as the voice disorder group)and 200 teachers without throat and voice disorders as the control group.The failure rate was significantly higher at 80 dB than 75 dB,and for the study group,the failure rate was higher than that of the control group.The difference was statistically significant in 1,2 and 10 minutes between the two groups(P <0.05).Conclusion Voice fatigue is very common in the primary school teachers and when high volumes are required,the voice fatigue is more serious.To reduce voice fatigue and the incidence of primary school teachers'voice disease,we should strengthen the teacher's voice health care.