1.The relationship between serum C-reactive protein levels in type 2 diabetic patients and periodontal diseases
Tao HAO ; Lin GAO ; Yan YANG ; Xin LIAO
Journal of Practical Stomatology 2001;0(03):-
Objective:To investigate serum high sensitive C-reactive protein levels in type 2 diabetic patients with periodontal diseases,and to explore the relationship between serum high sensitive C-reactive protein levels and periodontal diseases in type 2 diabetic patients.Methods:The study included 20 type 2 diabetic patients with moderate or severe periodontal diseases,20 type 2 diabetic patients without periodontal diseases and 20 normal as control.Serum hs-CRP,HbA1c and other biochemical parameters were detected.Results:Compared with normal control and type 2 diabetic subjects without periodontal diseases,serum hs-CRP levels in type 2 diabetic patients with moderate or severe periodontal diseases were significantly increased.Serum hs-CRP levels were significantly elevated in type 2 diabetic patients without periodontal diseases compared with normal control subjects(P
2.Effect of overexpressing isocitrate lyase on succinate production in ldh(-1) Corynebacterium glutamicum.
Chao YANG ; Ning HAO ; Ming YAN ; Lu GAO ; Lin XU
Chinese Journal of Biotechnology 2013;29(11):1696-1700
Corynebacterium glutamicum SA001 is a mutant with lactate dehydrogenase (ldhA) deletion. In order to increase metabolic flux from isocitrate to succinate, and to improve the production of succinate under anaerobic conditions,we transducted the gene aceA coding isocitrate lyase (ICL) from Escherichia coli K12 into Corynebacterium glutamicum SA001 (SA001/pXMJ19-aceA). After 12 h aerobic induction by adding 0.8 mmol/L of IPTG, the recombinant strain was transferred to anaerobic fermentation for 16 h. Succinate reached 14.84 g/L, with a productivity of 0.83 g/(L x h). Compared to C. glutamicum SA001, the activity of ICL of the recombinant strain was increased 5.8-fold, and the succinate productivity was increased 48%. Overexpression of isocitrate lyase will increase the metabolic flux of glyoxylate bypass flowing to succinate.
Corynebacterium glutamicum
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genetics
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metabolism
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Escherichia coli
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enzymology
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genetics
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Gene Deletion
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Industrial Microbiology
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Isocitrate Lyase
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biosynthesis
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genetics
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L-Lactate Dehydrogenase
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genetics
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Succinic Acid
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metabolism
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Transduction, Genetic
3.Nosocomial Infection: Investigation on 626 Cases
Wei DAI ; Xiutang CAO ; Haili LIN ; Jun GAO ; Lu HAO
Chinese Journal of Nosocomiology 2006;0(12):-
OBJECTIVE To investigate the present situation of the nosocomial infection in our hospital in order to decrease the infection rate. METHODS A total of 40 554 patients were inspected from Oct 2004 to Sept 2005.There were 626 patients with nosocomial infection. RESULTS The nosocomial infection was 1.54%.Old patients were the main components.The longer they were in hosptial,the more risk of infection they had.Elder monitoring department and hematological department were the most common infected units.Respiratory tract was the most common infected site of the body.The entity of disease for the most infection was malignant tumor.Invasive treatment was one of the causes for the infection. CONCLUSIONS The infection rate of our hospital was relatively low.We should establish the system to control the infection.Use the advanced statistical software well be to aid the control and discover the anti-infection deficiency from the statistical view-point.
4.Discussion of the subsidy mechanism of public hospitals in Zhejiang province
Yuhang ZENG ; Hengjin DONG ; Lin GAO ; Qian YANG ; Hao ZHANG
Chinese Journal of Hospital Administration 2017;33(2):96-97,95
Analyzed in the paper is the current subsidy mechanism for public hospitals in Zhejiang province, with analysis of problems found. The authors recommended to build a mass fraction subsidy mechanism to cover up the insufficiency of government financial subsidy; to fully leverage the price compensation of medical services; to subsidize in view of the functional positioning of various medical institutions;and explore more channels for public hospitals′subsidies.
5.Myogenic elephant skin cream combined with Vacuum sealing drainage (VSD) for the treatment of bedsores.
Yi-bin GAO ; Song-lin TONG ; Fang PAN ; Jian-hao YU
China Journal of Orthopaedics and Traumatology 2015;28(2):150-154
OBJECTIVETo discuss the efficacy of the myogenic elephant skin cream combining with VSD for treating severe bedsores.
METHODSTwenty-nine cases of III and IV degree bedsores were treated from June 2009 to June 2013. Among them, 15 cases were treated by myogenic elephant skin cream combined with VSD (VSD group) including 7 males and 8 females with an average age of (69.0±5.3) years old ranging from 17 to 96 years;other 14 cases were treated by the treatment of conventional dressing change (control group) including 6 males and 8 females with an average age of (71.0±4.2) years old ranging from 40 to 86 years. At 7, 14, 21, 28 d after treatments, specimens of wound two groups were respectively taken to examine immunohistochemical CD34 adopted SABC, the number of wild vascular cross were observed as capillary density value under high magnification microscope. It was used to assess the hyperplasia of granulation tissue of wound. The capillary density value and the visual wound observation were indicators for evaluation of clinical efficacy.
RESULTSSeventeen of 19 cases got complete data of specimens of wound at 7, 14, 21, 28 d,included 9 cases of VSD group,8 of control group. In the microscope view, the capillary density of VSD group was higher than that of control group significantly (P<0.05), it showed the application of VSD technology improved hyperplasia of granulation tissue much faster than conventional dressing change. In VSD group, 13 cases with 15 wounds healed, 2 cases with 3 wounds improved; in control group,3 cases with 3 wounds healed,7 cases with 9 wounds improved, 4 cases with 5 wounds were unhealed.
CONCLUSIONUsing the VSD technology with continuous high pressure suction to clean the drainage of wound and lacuna thoroughly can effectively control infection,promote the growth of granulation tissue, and then applying myogenic elephant skin cream to improve partial blood supply while prompting rapid growth of new granulation tissue and epithelial cell. The risk of this treatment is low,and the course of treatment is short, this provides a safe and effective method for treating bedsores.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Drainage ; methods ; Female ; Humans ; Male ; Middle Aged ; Pressure Ulcer ; therapy ; Skin Cream ; therapeutic use
6.Expression and correlation of Lewis y antigen and mucin 1 in ovarian epithelial tumor
Rui HOU ; Jian GAO ; Zhenhua HU ; Dawo LIU ; Yingying HAO ; Bei LIN ; Shulan ZHANG
Clinical Medicine of China 2013;(3):250-255
Objective To investigate the expression and the clinical significance of Lewis y antigen and Mucin 1 (MUC1),as well as to evaluate the correlation between them in epithelial ovarian tumor.Methods The expression of Lewis y antigen and MUC1 in 60 cases of epithelial ovarian malignant tumors,30 cases of borderline ovarian tumors,30 cases of benign ovarian tumors and 20 cases of normal ovarian tissues were detected by immunohistochemical staining.The relationship between Lewis y antigen and MUC1,and their relationship with biology characteristic of ovarian carcinoma were analyzed.An immunofluorescence double labeling methods was performed to detect the correlation between Lewis y antigen and MUC1.Results In malignant epithelial ovarian tumors,the positive rates of Lewis y antigen was 88.33%,which was significantly higher than the positive rates in borderline(60.00%,x2 =9.6405,P <0.01) and benign ovarian tumors(33.33%,x2 =28.8095,P <0.01) and normal ovarian samples (0,x2 =52.3457,P < 0.01).The positive rates of Lewis y antigen had nothing to do with the clinical pathological parameters of ovarian tumor,but the expression intensity of Lewis yantigen was increased with the development of the malignant degree(P < 0.05).The positive rates of MUC1 in malignant epithelial ovarian tumors was also significantly higher than that in borderline,benign ovarian tumors and normal ovarian samples (86.67% vs 53.33%,30.00%,25.00%,x2 =12.0321,29.4064,27.8464 ; P <0.01).And the expression intensity of MUC1 also increased with the development of clinical stage(P <0.01),but had nothing to do with the lymph node metastasis and histological grade(P > 0.05).In ovarian cancer,both Lewis y antigen and MUC1 were highly expressed,and their expression levels were positively correlated (r =0.707,P <0.01),and Lewis y antigen colocalized with MUC1.Conclusion Both Lewis y antigen and MUC1 are associated with the occurrence and development of ovarian cancer.Lewis y antigen and MUC1 might be a sigh of biological behavior in ovarian cancers,and this study provides theoretical evidence of ovarian cancer biological treatment.
7.Preliminary study of MR elastography in brain tumors
Lei XU ; Peiyi GAO ; Yan LIN ; Jiancheng HAN ; Zhinong XI ; Hao SHEN
Chinese Journal of Radiology 2008;42(6):605-608
Objective To investigate the potential values of magnetic resonance elastography (MRE)for evaluating the brain tumor consistency in vivo.Methods Fourteen patients with known solid brain tumor(5 male,9 female;age range:16-63 years)underwent brain MRE studies.Informed consent was obtained from all patients.A dedicated external force actuator for brain MRE study Was developed.The actuator was fixed to the head coil.During scan.one side of the actuator was attached to the patients'head.Low frequency oscillation Was produced by the actuator and caused shear waves propagating into brain tissue.The pulse sequence used in the study Writs phase-contrast gradient-echo sequence.Phase images of the brain were obtained and the shear waves within the brain were directly imaged.Phase images were processed with local frequency estimation (LFE) technique to obtain the elasticity image.Consistency of brain tumors Was evaluated at surgery and Was classified as soft,intermediate,or hard with comparison to the white matter of the brain.Correspondence of MRE evaluation with operative results was studied.Results The elastic modulus of the tumor Was lower than that of white matter in 1 patient,higher inll patients,and similar in 2 patients.At surgery,the tumor manifested a soft consistency in I patient,hard consistency in 11 patients,intermediate consistency in 2 patients.The elasticity of tumors in 14 patients evaluated by MRE was correlated with the tumor consistency on the operation.Conclusion MRE Can noninvasively display the elasticity of brain tumors in vivo,and evaluate the brain tumor consistency before operation.
8.The relationship between Fas expression and lung injury after gut ischemia-reperfusion injury.
Guo-lin GAO ; Yan-min LI ; Hui-bo AN ; Bao-cheng CHEN ; Hao-fu HU
Chinese Journal of Pediatrics 2003;41(10):773-774
Animals
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Lung
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immunology
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pathology
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Male
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Reperfusion Injury
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metabolism
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physiopathology
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Spleen
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immunology
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pathology
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fas Receptor
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analysis
9.Effect of the peri-implantitis on the biological function of osteoblasts obtained from the mandibles.
Hao WU ; Hong-chen LIU ; Lingling E ; Na LIU ; Bo ZHANG ; Yuxuan GAO ; Lin FENG
West China Journal of Stomatology 2014;32(2):130-133
OBJECTIVETo study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts.
METHODSPrimary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen I (Col I) mRNA levels were examined by real-time quantitative polymerase chain reaction. OCN protein levels were determined by Western blot.
RESULTS: After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue ( P <0.05). After 7 d of culture, OCN, Runx2, and Col I mRNA expression decreased ( P <0.05). The OCN protein levels also decreased ( P <0.05).
CONCLUSIONPeri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.
Bone and Bones ; Cell Differentiation ; Humans ; Mandible ; Osteoblasts ; Osteocalcin ; Peri-Implantitis ; RNA, Messenger
10.Case control study on open reduction internal fixation (ORIF) and minimally invasive percutaneous plate osteosynthesis (MIPPO) for the treatment of proximal humerus fractures in aged.
Yi-Bin GAO ; Song-Lin TONG ; Jian-Hao YU ; Wen-Jie LU
China Journal of Orthopaedics and Traumatology 2015;28(4):335-339
OBJECTIVETo investigate the clinical effects of close reduction and minimally invasive percutaneous plate osteosynthesis in treating proximal humerus fractures in the aged.
METHODSFrom February 2012 to December 2013,39 patients with proximal humerus fractures were treated with minimally invasive percutaneous plate osteosynthesis (MIPPO group, 21 cases) and open reduction internal fixation (ORIF group, 18 cases). Including 17 males and 22 females in the study, and aged from 67 to 88 years old with an average of (71.8 ± 5.2) years old. In MIPPO group, there were 11 males and 10 females with an average age of (70.0 ± 5.3) years old;and in ORIF group, there were 10 males and 8 females with an average age of (72.0 ± 4.2) years old. Operation time, blood loss during operation, fracture healing time and postoperative complications were recorded. The functions of the shoulder joints were assessed according to Constant-Murley score at final follow-up.
RESULTSAll the patients were followed up from 11 to 27 months with an average of 18.1 months. The mean blood loss of the MIPPO group was (176.0 ± 57.4) ml,while the ORIF group was (356.0 ± 66.9) ml (t = 7.22,P = 0.01). The operation time of the MIPPO group was (47.4 ± 14.9) min, while the ORIF group was (92.7 ± 15.8) min (t = 0.79, P = 0.03). Fracture healing time in the MIPPO group and ORIF group was (17.6 ± 5.8), ( 21.7 ± 4.9) weeks, respectively (P < 0.05). The mean Constant-Murley score at final follow-up was 89.7 ± 14.5 in MIPPO group, and 81.8 ± 13.2 in ORIF group (P < 0.05).
CONCLUSIONMIPPO has advantages of little trauma, less blood loss, rapid recovery, less vascular damage and so on and can effectively treat the proximal humerus fracture in the aged.
Aged ; Aged, 80 and over ; Bone Plates ; Case-Control Studies ; Female ; Fracture Fixation, Internal ; methods ; Humans ; Male ; Minimally Invasive Surgical Procedures ; Shoulder Fractures ; surgery