[Objective]To summarize the clinical experience of Professor FAN Binghua in treating sacroiliac joint dysfunction. [Method] From following Professor FAN Binghua's clinical and studying experienced cases ,summarizing the academic viewpoints and methods of Professor FAN Binghua in treating sacroiliac joint dysfunction, and with one classical case for experiencing details and methods when Professor FAN Binghua cures sacroiliac joint dysfunction. [Result]Based on theory of relationship between symptoms and etiology, Professor FAN comes up with concept of where there is symptom, there is reason and curing etiology should be put forward,starting with clinical symptoms to cure sacroiliac joint dysfunction, applying examinations of specialty to find out the internal close relationship between symptom and etiology and eliminate the similar disease, then curing the etiology in right way.[Conclusion]Pro.FAN has flexible thoughts, clear pertinence, and strong practicality, and histhree principls in cliniccan be guidence in clinic, and it is worth drawing reference and learning.

Objective To evaluate the growing condition of human umbilical vein endothelial cells (HUVECs), which were cultured on the membrane of different component, such as Chinese medicine, before or after the alkali treatment of 3-hydroxybutyrate-co-3-hydroxyhexanoate copolyesters (PHBHHx) and the biocompatibility between PHBHHx flim and endothelial cell. Methods The HUVECs were harvested by Baudine method,and identified by immunohistochemical method.Then the HUVECs of third passage were inoculated on the material surface and cultured for 8 hours,12 hours and 24 hours. After that, the morphology of HUVECs on different surfaces were observed by scanning electron microscopy, the distribution condition on different membranes were compared by cell-labeling immunofluorescence, and the cell viabilities of all groups were detected by MTT method. Results The HUVECs were successfully separated,and immunohistochemistry staining of FLK-1 and factor Ⅷ was positive.The result of HUVECs culture showed that cells on the material surface growed well, and proliferated significantly. The MTT analysis showed that the PHBHHx film of surface modification and adding some certain proportion of Chinese medicine could promote the growth and proliferation of HUVECs in vitro,and the cells were thriving, full shape, distribution on the surface by scanning electron microscopy and fluorescence microscopy.Conclusion The PHBHHx film of surface modification and containing certain proportion of Chinese medicine coating had good compatibility of HUVECs, which was favourable to cell growth, adherence and proliferation in vitro.

Objective:To investigate the similarities and differences of chondrolaryngoplasty between female and transgender women.Methods:We enrolled 23 patients with chondrolaryngoplasty in Peking University Third Hospital from March 2016 to September 2019, with age from 18- 37 y/o (average at 26. 87±5. 54 y/o) ; employing eleven-point Likert scale to conduct an average 14mths follow-up. We assigned patients into female (n=11) and transgender women(n=12) groups, using SPSS 22. 0 tool to process and analyze data. Paired sample t test is conducted to compare pre- and postoperative differences, while independent sample t test is to compare between groups; p value less than 0. 05 is considered to have statistical significance.Results:Satisfaction with surgery is 95. 65% (22/23 patients) ; satisfaction with appearance of laryngeal prominence is 82. 61% (19/23 patients ). After operation, the Self-Estimate Laryngeal Prominence Score improves from 6. 57±2. 45 to 2. 22±2. 09; the Social Ability Restriction Score improves from 6. 30±3. 02 to 1. 52±2. 54; the Phycological Burden Score decrease from 7. 70±2. 44 to 1. 83±2. 62; all data have statistical significances ( P<0. 01). The improvement of previous two scores is greater in transgender women group with statistical significances ( P< 0. 05). Conclusions:Chondrolaryngoplasty can remarkably improve the appearance of laryngeal prominence of patients; Transgenders women can obtain better social and phycological improvement than female patients after chondrolaryngoplasty.

Objective:To investigate the similarities and differences of chondrolaryngoplasty between female and transgender women.Methods:We enrolled 23 patients with chondrolaryngoplasty in Peking University Third Hospital from March 2016 to September 2019, with age from 18- 37 y/o (average at 26. 87±5. 54 y/o) ; employing eleven-point Likert scale to conduct an average 14mths follow-up. We assigned patients into female (n=11) and transgender women(n=12) groups, using SPSS 22. 0 tool to process and analyze data. Paired sample t test is conducted to compare pre- and postoperative differences, while independent sample t test is to compare between groups; p value less than 0. 05 is considered to have statistical significance.Results:Satisfaction with surgery is 95. 65% (22/23 patients) ; satisfaction with appearance of laryngeal prominence is 82. 61% (19/23 patients ). After operation, the Self-Estimate Laryngeal Prominence Score improves from 6. 57±2. 45 to 2. 22±2. 09; the Social Ability Restriction Score improves from 6. 30±3. 02 to 1. 52±2. 54; the Phycological Burden Score decrease from 7. 70±2. 44 to 1. 83±2. 62; all data have statistical significances ( P<0. 01). The improvement of previous two scores is greater in transgender women group with statistical significances ( P< 0. 05). Conclusions:Chondrolaryngoplasty can remarkably improve the appearance of laryngeal prominence of patients; Transgenders women can obtain better social and phycological improvement than female patients after chondrolaryngoplasty.

Objective: To study the effect and mechanism of Shuanghua aerosol (SHA) in treating infantile upper respiratory tract infection. Methods: In the clinical study, 276 patients were randomly divided into two groups. The treated group was treated with SHA and the control group was treated with Shuanghuanglian aerosol (SHLA, 双黄连气雾剂). In the experimental study, the effects of SHA on anti-inflammation and anti-virus were observed. Results: The clinical total effective rate of SHA was 99.03% and its cure rate 65.38%, while those of the SHLA were 94.11% and 44.12% respectively, significant difference was shown between the two groups (P<0.01). Experimental study showed that SHA could inhibit the xylol induced ear swelling and the egg white induced paw swelling in mice obviously, and inhibit the proliferation of influenza virus in rat's lung. Conclusion: SHA has obvious anti-inflammatory and anti-viral effect, and has good curative effect in treating infantile upper respiratory tract infection.

Biomarkers, Tumor ; analysis ; Fatty Acid-Binding Proteins ; analysis ; Humans ; Receptors, Retinoic Acid ; analysis ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Two-Dimensional Difference Gel Electrophoresis ; methods ; Up-Regulation ; Urinary Bladder Neoplasms ; chemistry

OBJECTIVETo explore the method of predicting the stage of prostate cancer with serum prostate-specific antigen (PSA) and pathological grade.

METHODSOne hundred and eighty-seven patients were studied retrospectively with prostate cancer diagnosed by systemic biopsy in our hospital. The rank correlation analysis, rank sum test and stepwise discriminant multivariate analysis were used to assess the correlation of serum PSA level, ratio of free PSA to total PSA (FPSA/TPSA ratio) with Gleason score (GS) and stage.

RESULTSSerum PSA level increased with GS for prostate cancer patients (r = 0.369, P < 0.001). With increasing stage, serum PSA level and GS increased (r = 0.398, 0.530, P < 0.001). Overall, FPSA/TPSA ratio was not correlated with stage (P > 0.70), but a significant negative correlation was demonstrated between them when serum PSA < or = 10 microg/L (r = -0.600, P < 0.05). When serum PSA > 20 microg/L, 67% - 87% patients with prostate cancer may be stage C or D. The equation using serum PSA and GS to predict the stage of patients with prostate cancer was: x = -3.488 + 0.041 x PSA + 0.428 x GS.

CONCLUSIONSSerum PSA level is positively correlated with GS for prostate cancer patients. Serum PSA level and GS are positively correlated with stage. A negative correlation between FPSA/TPSA ratio and stage is demonstrated when serum PSA < or = 10 microg/L. The combination of serum PSA and GS may predict the stage of patients with prostate cancer.

Adult ; Aged ; Aged, 80 and over ; Humans ; Male ; Middle Aged ; Multivariate Analysis ; Neoplasm Staging ; methods ; Prostate-Specific Antigen ; blood ; Prostatic Neoplasms ; blood ; pathology ; ROC Curve ; Retrospective Studies ; Sensitivity and Specificity

This study was purposed to detect the expression of autophagy-related gene Beclin1 and microtubule-associated protein 1 light chain 3 (MAPLC3) in bone marrow mononuclear cells (BMMNC) isolated from acute leukemia (AL) patients, and to explore its significance. Transmission electron microscopy and RT-PCR were used to detect the autophagy activity and the expression level of Beclin1 and MAPLC3 mRNA in BMMNC isolated from 27 AL patients with de novo, refractory or relapse AL and completely remission and 31 normal persons respectively. The results showed that autophagy activity and expression levels of Beclin1 and MAPLC3 mRNA in BMMNC from de novo AL patients were 80%, 0.68 ± 0.18, 0.24 ± 0.06, respectively; those in BMMNC from refractory or relapse AL patients were 100%, 0.79 ± 0.09, 0.30 ± 0.07, respectively; those in BMMNC from CR patients were 40%, 0.52 ± 0.15, 0.16 ± 0.04, respectively, while those in BMMNC from normal persons were 20%, 0.57 ± 0.13, 0.16 ± 0.05, respectively. The autophagic activity and expression levels of Beclin1 and MAPLC3 mRNA in de novo and refractory or relapse AL patients were higher than those in normal persons, with statistical significance (p < 0.05), while the comparison between CR patients and normal control showed no statistical difference (p > 0.05). It is concluded that autophagy activity and Beclin1 and MAPLC3 mRNA expression level of in de novo and refractory or relapse patients are higher than those in normal control, and the up-regulation of autophagy activity and expression of Beclin1 and MAPLC3 mRNA in refractory or relapse patients is especially significant. This may be related to the genesis, development and drug resistance of AL.
Acute Disease ; Adolescent ; Adult ; Aged ; Apoptosis Regulatory Proteins ; metabolism ; Beclin-1 ; Bone Marrow Cells ; metabolism ; Child ; Female ; Humans ; Leukemia ; metabolism ; pathology ; Male ; Membrane Proteins ; metabolism ; Microtubule-Associated Proteins ; metabolism ; Middle Aged ; RNA, Messenger ; genetics ; Young Adult

Objective To develop a system to rescue virus by intracellular expression of T7 RNA Polymerase. Methods The gene of T7 RNA Polymerase was amplified and cloned to VR1012 by molecular biological technology. The expression plasmid VR-1a was then identified. VR-1a and EV71 infectious plasmid were co-transfected in Veto cell. CPE was observed and viral gene viral antigen were detected.Results The gene of T7 RNA Polymerase was successfully cloned into vector VR1012. Vere cell developed to CPE after being transfected VR-1a and EV71 infectious plasmid. EV71 gene was amplified by RT-PCR from the culture. EV71 antigen was also detected by ELISA. Conclusion The method can be used to rescue virus. It could apply to immunologic research of EV71 DNA vaccine.

Objective To construct the recombinant plasmid containing S1 gene of new type of reovirus,and to study the expression of protein σ1 in Vero cells.Methods The recombinant plasmid,named pC-S,was constructed by cloning S1 gene into vector pCAGGS/MCS.Then Vero cells were transfected with pC-S and collected at 24,48,72 h post transfection followed by SDS-PAGE and Western-Blot assay.Results Results Both SDS-PAGE and Western-Blot assay indicated that σ1 protein could be expressed well and the highest expression level was 72 h post transfection.Conclusions σ1 protein could be expressed well in Vero cells by transfected with recombinant plasmid containing S1 gene,and could give some implications for subsequent research on virus-host interactions.