1.Role of Apoptosis in Acute Rejection of Pancreaticoduodenal Transplantation in Rats
Chinese Journal of Bases and Clinics in General Surgery 2004;0(01):-
Objective To investigate the roles of cell apoptosis and the gene expressions of Fas, FasL, bcl-2 and bax in acute rejection of pancreaticoduodenal transplantation and to evaluate the function of duodenum biopsy for early detection of rejection in rats. Methods Wistar and SD rats were divided into two groups: ①Wistar rats that underwent allogenic pancreaticoduodenal transplantation from the organs of SD rats; ②Wistar rats that received homogenic transplantation. The grafts were then harvested on day 3, 5 and 7 after the transplantation, and all graft samples were observed with HE staining and TUNEL was also used to detect apoptotic cells. The expressions of Fas, FasL, bcl-2 and bax were measured by immunochemical method. According to Nakhleh’s score, pathologic features of transplanted pancreas and duodenum were ranged from one to three scores in order. Results The percentage of same or different scores between the pathological scores of pancreas and duodenum in allogenic pancreaticoduodenal transplantation group were 61.1% (11/18) and 38.9% (7/18) respectively, and there were 6 specimens of pancreatic tissue got higher scores with only one higher score for duodenum. There were significant differences of histopathologic rejection scores and apoptotic indices between the two groups, respectively (P
2.Effect of relation between ionization chamber and photographic position on X-ray image quality
Yong HAO ; Xiancheng CUI ; Jun LUO
Chinese Medical Equipment Journal 1993;0(05):-
With the introduction of the principle of automatic exposure control system,this paper discusses the relation between ionization chamber and photographic position,and analyzes its effect on X-ray image quality.
3.Diagnostic Value of MR Diffusion Weighted Imaging and CT and MR Perfusion Imaging in Patients with Different Degrees of Liver Cirrhosis
Jun LI ; Xuewei HUANG ; Lin YANG ; Peng LUO ; Hao CHEN
Progress in Modern Biomedicine 2017;17(25):4940-4943,4895
Objective:To study the diagnostic value of MR diffusion weighted imaging (DWI) and CT and MR perfusion imaging in patients with different degrees of liver cirrhosis.Methods:A total of 60 patients with liver cirrhosis,who were treated in Ziyang Hospital of West China Hospital of Sichuan University from August 2015 to February 2017 were selected.According to the Child-Pugh classification,32 cases of grade A were mild cirrhosis (denoted as group A),16 cases of grade B and 12 cases of grade C were moderate to severe cirrhosis (denoted as group B),30 healthy volunteers were selected as group C in the same period.All the subjects in the three groups were examined by DWI,CT and MR perfusion imaging.The ADC value,hepatic portal perfusion ratio [SSr (CT) and SSr (MR)] were compared among the three groups,and Spearman correlation analysis was used to analyze the correlation between the indexes.Results:There was no significant difference in ADC values among the three groups (P>0.05),and the ADC values of group A and B were lower than those of group C,but the difference was not statistically significant (P>0.05).There was no significant difference in ADC values between group A and B (P>0.05).The levels of SSr (CT) and SSr (MR) in the three groups were compared,the difference was statistically significant (P<0.05);the levels of SSr (CT) and SSr (MR) in group B were significantly higher than those in group A and C,the difference was statistically significant (P<0.05).There was no significant difference in the levels of SSr (CT) and SSr (MR) in group A and C (P>0.05).Spearman correlation analysis showed that SSr (CT) was positively correlated with SSr (MR) in the patients with liver cirrhosis (r=0.687,P=0.000).Conclusion:CT and MR perfusion imaging can reflect the lesion degree of liver cirrhosis,and diagnostic effects of the two are better than DWI imaging,which is worthy of clinical promotion.
4.IPS e. max Press porcelain crown in esthetic restoration of anterior teeth:a follow-up of marginal adaptation and color match
Yuan LUO ; Juan LUO ; Jun ZHOU ; Xueheng LIU ; Yanhong HAO ; Zhen FANG
Chinese Journal of Tissue Engineering Research 2015;(25):4017-4021
BACKGROUND:IPS e. max Press porcelain crown has been widely used in clinical restoration because of its aesthetic effect and biological properties. OBJECTIVE:To explore the effect of IPS e. max Press porcelain crown in esthetic restoration of anterior teeth in terms of marginal adaptation and color match. METHODS:Total y 52 patients with 138 defective anterior teeth (20 males and 32 females, 18-45 years old, including 62 anterior tooth defects, 46 tooth traumas, 30 denticles) were divided into two groups randomly:treatment group (26 cases) and control group (26 cases) were given IPS e.max Press porcelain crown and conditional Co-Cr al oy handcuffed ceramic crowns, respectively. Al the cases were asked for a second visit in 1 week after restoration, and the integrity of ceramic veneer, marginal adaptation, gingival health and color match were evaluated during 6-month and 2-year fol ow-ups. RESULTS AND CONCLUSION:The degrees of satisfaction on the color, shape and adaptation in the treatment group were al higher than those in the control group (P<0.05). At 6 months after restoration, the marginal adaptation, gingival health and color match in the treatment group were al better than those in the control group (P<0.05). At 2 years after restoration, there was also a significant difference in the marginal adaptation, gingival health and color match between the two groups (P<0.05). IPS e.max Press porcelain crown has the advantages of aesthetics and biological characteristics, so it can be used as an ideal restoration method for esthetic restoration of anterior teeth.
5.The study of cognitive function and synaptic plasticity in depressed rats following electroconvulsive shock
Ping LI ; Xuechao HAO ; Feng LV ; Ke WEI ; Jie LUO ; Jun CAO ; Jun DONG ; Su MIN
Chinese Journal of Nervous and Mental Diseases 2014;(12):715-720
Objective To investigate the role of autophagy and synaptophysin (SYP) in cognitive impairment in de?pressed rats receiving electroconvulsive shock (ECS). Methods Clean and healthy adult male Sprague-Dawley rats were acclimatized to a standard laboratory environment for 7 days. The chronic unpredictable mild stress (CUMS) was used to establish the rat model of depression. Behavior tests were conducted before and after CUMS to evaluate the depression and cognition level of rats. After establishment of the model, 24 rats were randomly divided into ESC group (group E) and depression group (group D) with 12 rats in each group. The rats in group E were administered 80 mg/kg of propofol (10 mg/mL) by intraperitoneal injection, followed by ECS treatment. The rats in group D were administered propofol by intra?peritoneal injection, followed by sham-ECS treatments. The above interventions were conducted daily for 7 consecutive days. After the interventions, rats underwent behavior tests as before. Subsequently, rats were killed and specimens were collected for measurements. Immunohistochemistry was performed to examine autophagy markers such as Beclin 1 and LC3Ⅱand ELISA was used to detect SYP in the hippocampus. Results Group E after ECS significantly increased the percentage of sucrose preference (68.2%±8.7%), rearing times (7.0±1.9), total horizontal distance [(569.5±70.0) cm], es? cape latency [(21.9±5.3)s] and space exploration time [(20.5±3.9)s] compared with group D or group E before ECS. There was no significant difference in these index between groups before ECS or in group E between before and after ECS(P>0.05). Compared with group D, group E had upregulated protein expression levels of Beclin 1 and LC3Ⅱin CA1, CA3, DG as well as the area near the hippocampus and increased SYP contents (P<0.05). Conclusions Cognitive impairment in depression rats following ECS correlates with activated autophagy and increased SYP by ECS.
6.Construction of automatic elucidation platform for mechanism of traditional Chinese medicine.
Bai-xia ZHANG ; Si-jun LUO ; Jing YAN ; Hao GU ; Ji LUO ; Yan-ling ZHANG ; Ou TAO ; Yun WANG
China Journal of Chinese Materia Medica 2015;40(19):3697-3702
Aim at the two problems in the field of traditional Chinese medicine (TCM) mechanism elucidation, one is the lack of detailed biological processes information, next is the low efficient in constructing network models, we constructed an auxiliary elucidation system for the TCM mechanism and realize the automatic establishment of biological network model. This study used the Entity Grammar Systems (EGS) as the theoretical framework, integrated the data of formulae, herbs, chemical components, targets of component, biological reactions, signaling pathways and disease related proteins, established the formal models, wrote the reasoning engine, constructed the auxiliary elucidation system for the TCM mechanism elucidation. The platform provides an automatic modeling method for biological network model of TCM mechanism. It would be benefit to perform the in-depth research on TCM theory of natures and combination and provides the scientific references for R&D of TCM.
Animals
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Automation
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instrumentation
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methods
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Databases, Factual
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Gene Regulatory Networks
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Humans
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Medicine, Chinese Traditional
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Plants, Medicinal
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chemistry
7.Relationship of IL-8/-251 gene polymorphisms with incidence of sepsis in patients with severe traumatic injury
Jiang HAO ; Qi WENG ; Jishen LUO ; Jun LIU ; Minghao YANG ; Ping ZHANG
Chinese Journal of Trauma 2013;29(12):1220-1223
Objective To assess the clinical relevance of polymorphisms at position-251 in the promoter region of IL-8 gene and the incidence of sepsis in patients with severe trauma.Methods A total of 296 patients with severe trauma were included in the prospective cohort study.Incidence of sepsis was decided on the basis of the clinical manifestations and blood culture results.Muhiple organ dysfunction score (MODS) was performed using Marshall' s standard.IL-8/-251 gene polymorphisms were genotyped using restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP).IL-8 plasma level was determined using ELISA method.Results Genotype frequency at IL-8/-251 locus in trauma patients was in accord with Hardy-Weinberg equilibrium (P > 0.05).Sepsis incidence in trauma patients with TT,TA,and AA genotypes at IL-8/-251 locus was 58.1%,49.6%,and 25.0% respectively.Multiple regression analysis showed inverse correlation between sepsis incidence and quantity of A alleles [OR =0.637,95% CI (0.421,0.963),P < 0.05].Carriers of AA genotype presented lower MODS score than those of other two genotypes (P < 0.05).IL-8 plasma level presented significant difference among carriers of the three genotypes (P < 0.01) and A alleles were associated with the down-regulation of IL-8 (P < 0.01).Conclusion IL-8/-251T/A polymorphisms are implicated in the development of posttraumatic sepsis and AA genotype is protective against posttraumatic sepsis.
8.Efficacy of laparoscopy-assisted radical gastrectomy for gastric cancer: a report of 726 cases
Peiwu YU ; Feng QIAN ; Yingxue HAO ; Yongliang ZHAO ; Yan SHI ; Bo TANG ; Huaxing LUO ; Jun CHEN
Chinese Journal of Digestive Surgery 2011;10(1):44-47
Objective To explore the efficacy of laparoscopy-assisted radical gastrectomy for patients with gastric cancer. Methods The clinical data of 726 patients who received laparoscopy-assisted radical gastrectomy for gastric cancer at the Southwest Hospital from January 2004 to April 2010 were retrospectively analyzed. The operation time, operative blood loss, number of lymph nodes dissected, length of hospital stay and mobidity were evaluated using t test or chi-square test. The survival of the patients were evaluated by Kaplan-Meier method.Results Laparoscopy-assisted radical gastrectomy was successfully carried out on 707 patients, and 19 patients were converted to open surgery. The mean operation time, operative blood loss, number of lymph nodes dissected were (179 ±52)minutes, (87 ±51) ml and 33 ± 14, respectively. The average distances of proximal and distal resection margin to the tumors were (6.3 ± 1.9)cm and (5.6 ± 1.7)cm, respectively. The average time to flatus, time to fluid diet and length of hospital stay were (2.9 ± 1.4) days, (3.1 ± 1.7) days and (7.9 ± 3.5) days,respectively. The peri- and postoperative mobidities were 2.2% (16/726) and 4.0% (29/726), respectively. A total of 685 patients were followed up for 6-82 months (mean, 48.3 months), and the 5-year survival rate was 58.4%. Conclusions Laparoscopy-assisted radical gastrectomy is a feasible procedure with minimal trauma, low morbidity and quick recovery of patients.
9.Expression and clinical significance of survivin in transitional cell carcinoma of bladder
Baiye JIN ; Jindan LUO ; Jun FAN ; Hao PAN ; Dan XIA ; Hainu GAO ; Songliang CAI
Chinese Journal of Urology 2009;30(9):606-608
cuses tumors. Survivin seems to be a promising marker for analyzing clinical stages and predicting the prognosis of TCC.
10.Procedure of seed quality testing and seed grading standard of Prunus humilis.
Hao WEN ; Guang-Xi REN ; Ya GAO ; Jun LUO ; Chun-Sheng LIU ; Wei-Dong LI
China Journal of Chinese Materia Medica 2014;39(21):4191-4196
So far there exists no corresponding quality test procedures and grading standards for the seed of Prunus humilis, which is one of the important source of base of semen pruni. Therefor we set up test procedures that are adapt to characteristics of the P. humilis seed through the study of the test of sampling, seed purity, thousand-grain weight, seed moisture, seed viability and germination percentage. 50 cases of seed specimens of P. humilis tested. The related data were analyzed by cluster analysis. Through this research, the seed quality test procedure was developed, and the seed quality grading standard was formulated. The seed quality of each grade should meet the following requirements: for first grade seeds, germination percentage ≥ 68%, thousand-grain weight 383 g, purity ≥ 93%, seed moisture ≤ 5%; for second grade seeds, germination percentage ≥ 26%, thousand-grain weight ≥ 266 g, purity ≥ 73%, seed moisture ≤9%; for third grade seeds, germination percentage ≥ 10%, purity ≥ 50%, thousand-grain weight ≥ 08 g, seed moisture ≤ 13%.
Cluster Analysis
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Germination
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Prunus
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growth & development
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Seeds
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physiology