Aim To investigate the reversal effect of PARP-1 inhibitor PJ34 on cisplatin-resistance in hu-man lung adenocarcinoma A549/DDP cells and the mechanism. Methods A549/DDP cells were treated with PJ34 alone or combined with cisplatin. The effects of proliferation inhibition were assayed by MTT meth-
od. The apoptosis ratios of cells were analyzed by flow cytometry. The protein expression of PARP-1 and LRP, GST-π were measured by Western blot assay. Results PJ34 could inhibit the proliferation of A549/DDP cells alone. The non-toxic dose of PJ34 could signifi-cantly resensitize A 5 4 9/ DDP cells to cisplatin , induce
apoptotic,lower the expression of PARP-1 and resist-ance-associated protein LRP and GST-π. Conclusion PJ34 could inhibit the proliferation of A549/DDP cells and resensitize A549/DDP cells,partially reverse cisplatin-resistance in A549/DDP cells, with a proba-
ble mechanism relating to increased apoptotic rate,and lowered expression of PARP-1 and resistance-associat-ed protein LRP and GST-π.

BACKGROUND:The implantable artificial pacemaker that needs long-term wear is in direct contact with human body tissue, and thus, biocompatibility of the materials is very important. OBJECTIVE:To investigate the material progress and material-related complications of implantable artificial pacemakers. METHODS:Articles related to implantable artificial pacemaker materials and related complications were searched in Wanfang database and PubMed database from 2000 to 2015. The keywords were pacemaker; materials; pacemaker leads; pacemaker electrode; complication in Chinese and English, respectively. Finaly, 27 articles were included and summarized. RESULTS AND CONCLUSION: The implantable artificial pacemakers are in direct contact with the body tissues through the titanium alloy shell covering the pulser, the electrode wire covering the silicone, polyurethane, epoxy resin, and the electrode materials (including carbon, platinum-iridium alloy, Elgilog alloy). These materials have good physical and chemical properties as well as good cytocompatibility, electrophysiological compatibility and blood compatibility with the human body that have been confirmed in the past decades. However, material-related complications often occur, such as contact dermatitis, wire breakage, dislocation, cardiac perforation, venous thrombosis and tricuspid regurgitation along the wire, capsular infection and hematoma when placing the pulser. With the rapid development of today's microelectronic technology, research on the modification of pacemaker materials and the pacing system has been ongoing and has made considerable progress. Moreover, wireless pacemaker development and clinical application may be the future direction of development.

Objective To determine the effect of using cytomegalovirus-seronegative blood components in preventing transfusion-acquired cytomegalovirus infection,which laid foundation for the application of blood antibody screening of cytomegalovirus.Methods The documents of studies about the comparison in transfusion-acquired cytomegalovirus ratio between using cytomegalovirus-seronegative blood components with using cytomegalovirus-unscreened /non-WBC-reduced blood were retrieved from the databases of PubMed,MEDLINE,Ovid,ProQuest,EBSCO,Cochrane Library,EMbase,CNKI,VIP,CBM and WanFang Library,and the reference in studies were retrieved by hands at the same time.The documents were screened,extracted and evaluated according to inclusion and exclusion criteria,and then given a Meta-analysis by using Rev Man 5.1 software.Results There were totally 7 controlled studies(430 patients) included.The results of Meta-analysis showed that compared with using cytomegalovirus-unscreened/non-WBC-reduced blood,the effect of using cytomegalovirus-seronegative blood components in preventing transfusion-acquired cytomegalovirus infection had a statistical difference(OR=0.07,95%CI:0.03-0.18,P<0.01).Conclusion Application of blood antibody screening of cytomegalovirus is effective in preventing transfusion-acquired cytomegalovirus infection,especially organ transplantation and neonate patients.

BACKGROUND:Transforming growth factor-βhas been shown to exert an obvious induction effect on the differentiation of bone marrow mesenchymal stem cells into chondrocytes. Cyclical tensile strain simulates mechanical environment of chondrocytes in the body, and plays an important regulatory role in cellproliferation and differentiation. OBJECTIVE:To discuss the synergy of transforming growth factor-βand cyclical tensile strain in inducing the differentiation of bone marrow mesenchymal stem cells into chondrocyte-like cells. METHODS:A total of 10 2-month-old New Zealand rabbits were selected. Bone needle was used to penetrate the medul ary cavity of bone. 3.0-4.0 mL of bone marrow was extracted for isolation and culture of bone marrow mesenchymal stem cells. Passage 3 cells were randomly assigned to four groups:blank, transforming growth factor-β, cyclical tensile strain and cyclical tensile strain+transforming growth factor-βgroups. After 1, 3 and 6 days, cells were obtained. General morphology was observed using safranin O staining. Glycosaminoglycan levels were detected by alcian blue staining. Matrix metal oproteinase-13 and tissue inhibitor of metal oproteinase-1 levels in supernatant were measured using ELISA. Type II col agen, matrix metal oproteinase-13 and tissue inhibitor of metal oproteinase-1 mRNA relative expression was detected using RT-PCR. RESULTS AND CONCLUSION:Safranin O staining showed fusiform or irregular triangular cells. cellnumber
and matrix secretion increased in each experimental group than in blank group. Glycosaminoglycan levels in the supernatant were greater in the transforming growth factor-βand cyclical tensile strain+transforming growth factor-βgroups than in the blank group (P<0.05). Type II col agen mRNA relative expression was higher in the cyclical tensile strain+transforming growth factor-βgroup than in the blank group (P<0.05). Results indicated that transforming growth factor-βand cyclical tensile strain could induce the differentiation of bone marrow mesenchymal stem cells into chondrocytes, showing an apparent cooperative action.

To measure the relative affinity and type Specificity of neutralizing monoclonal antibodies against the hexon antigen of adenovirus type 7,a simple ELISA double antibody binding system has been developed.By this method,ralative affinity of nine MAbs was estimated from the antibody concentrations at approximately 50% of plateau binding and were ranked.The theoretical basis for this method was discussed. Hybridomas secreting antibodies of desired affinity can be selected at an early stage after fussion by measuring relative affinity of hybridomas supernatants.The type specificity was judged by the difference between end-point concentration against adeno- virus type 7 and type 3.And the relation between affinity and specificity of MAbs was discussed.

Objective Of all the knee ligament,the anterior cruciate was totally disrupted the most frequently.Although a high degree of success and satisfactory results of replacement of anterior cruciate liga-ment with autograft tendon by arthroscopy were demonstrated,the objective of the investigation was to retro-spectively analyze miscellaneous pitfalls in anterior cruciate ligament reconstruction procedure and present its treatment.Methods Between January1995and June2002,consecutive194male and female patients between thirteen and fifty-eight years of age who sought treatment for a chronic tear of the anterior cruciate ligament were treated by arthroscopic assisted anterior cruciate ligament reconstruction.The time-interval between the index injury and the surgery was from1.5to37months.The diagnosis of anterior cruciate liga-ment injury was established by Lachman test,pivot-shift examination and MRI.The procedures included au-tograft bone-patellar tendon-bone replacement fixed with Kurosaka interference screws in75cases,allograft bone-patellar tendon-bone with Kurosaka interference screws in13cases,autograft bone-patellar tendon-bone fixed with Aesculap Endobutton suture plates in11cases,allograft bone-patellar tendon-bone with Aesculap Endobutton suture plates in6cases,hamstring autografts fixed by Aesculap Endobutton suture plate in89cases.Results There were39intraoperative pitfalls happened in36cases,in which2intraop-erative complications were unsalvageable.The causes of pitfalls could be classified into tunnel,graft materi-als,fixation and other problems.The most common pitfalls were the mistakes of position and direction of guide wires for bone tunnel occurred in8cases.The interference screw was loosened in2cases.Bending of the guide wire happened in3cases.Short and bifurcated of harvested grafts were the most common problem in reconstruction with hamstring autografts and Aesculap Endobutton suture plate technique.In the present,there were no complications of patellar fracture and breakage of reconstructive materials.Conclusion A deep knowledge and technique are necessary to determine and perform in anterior cruciate ligament recon-struction procedure,only if mastering the essentials of different anterior cruciate ligament reconstruction methods and handling flexibly,could an orthopedist cope with miscellaneous pitfalls rightly.

Objective To study the correlation between senile mild cognitive impairment (MCI) and metabolize of oxygen-derived free radicals, acetylcholinesterase(AchE), blood lipids and inflammatory mediator. Methods Memory,the levels of superoxide dismutase (SOD), malondialdehyde (MDA), AchE, blood lipids, IL-1? and IL-6 of MIC group(45 cases) and elder healthy group(45 controls) were measured, funther compare and correlative analysis were made.Results Compared with elder healthy group, memory score in each item and memory quotient in MCI group were significantly lower (allP

Objective To explore the causes and treatments for complications of laparoscopic incisional hernia repair.Methods A total of 110 patients with incisional hernia(42 men and 68 women,aged 33 to 89 years with a mean of 65)were treated with laparoscopic mesh repair in our hospital from March 2004 to May 2007.The clinical records of the patients were analyzed retrospectively.Results The laparoscopic repair was completed in all of the patients expect in one,who was converted to open surgery because of intraperitoneal adhesion.After the operation,15(13.6%)patients developed abdominal distension,86(78.2%)had severe pain in the operative area(not less than 6 weeks in 2 cases),23(20.9%)had seroma,6(5.4%)had urinary retention,2(1.8%)had acute gastric dilation,2(1.8%)had intestinal fistula,2(1.8%)had respiratory dysfunction,and 2(1.8%)showed recurrence of the hernia.Conclusion Most of the complications of laparoscopic incisional hernia repair are caused by insufficient preoperative preparation,intraperitoneal adhesion,difficulty in exposure of the visual field,improper use of electrocoagulation,small-sized masses,or weak fixation.

ObjectiveTo observe the changes of expression of aquaporin-1(AQP-1)and AQP-4 in drowned and postmortem immersed rats’lungs.MethodsThirty healthy male Wistar rats were randomly divided into drowning group, postmortem immersion group and cervical dislocation group. The morpho-logical changes of rats’lungs were observed using HE staining. The mRNA and protein expressions of AQP-1 and AQP-4 in rats’lungs were detected by real-time PCR, immunohistochemistry and Western blotting, respectively.ResultsThe results of immunohistochemistry and the Western blotting showed that the protein expression of AQP-1 of the drowning group was higher than the postmortem immersion group and the cervical dislocation group(P<0.05). The result of immunohistochemistry showed that the protein expression of AQP-4 of the drowning group was higher than the postmortem immersion group and the cervical dislocation group(P<0.05)while no difference were detected among the three of them by Western blotting(P>0.05). The mRNA expressions of AQP-1 and AQP-4 in rats’lungs of the drown-ing group was significantly higher than the postmortem immersion group(P<0.05).ConclusionThe in-crease of mRNA and protein expressions of AQP-1 and AQP-4 in lungs of rats with cute lung injury of the drowning group would be useful for differentiating vital drowning from postmortem immersion.

Objective To describe the surgical technique of arthroscopic reconstruction of anterior cruciate ligament with quadrupled stranded semitendinosus tendon and suture plate, and to review the short term outcome of its clinical application. Methods Fifty one cases of chronic anterior cruciate ligament ruptures were reconstructed arthroscopically with quadrupled stranded semitendinosus tendon. During the operation the harvested semitendinosus tendon was cut into two segments of equal length, and then folded to four strands. The proximal end of the graft was fixed to a small plate that lied over the orifice of the femoral tunnel, and the distal end was fixed to a button that covered the orifice of the tibial tunnel. The patients were followed up for 12-24 months and were rehabilitated according to a special plan. The range of motion and stability of the knee, the muscle function of the thigh, the knee function in daily activities, and the ability for running were evaluated periodically. The influence of the combined meniscal and cartilage lesion on the restoration of knee function was also studied. Results The ranges of motion of the injured knee were regained in all cases three months after operation. The quadriceps/hamstrings peak torque ratio reached more than 90% of the uninjured leg two years after operation. The results of KT-1000 examination showed that the anterior stability of the knee after anterior cruciate ligament reconstruction decreased slightly with time, but in 73.8 percent of all cases the anterior stability of the injured knee were equal or better than the uninjured knee. According to Lysholm knee scale score, the average knee score were 56.7?7.4 and 98.5?0.3 respectively before and two years after operation, and the difference was of statistical significance. The average knee score of the group with cartilage lesion was 96.44?2.36, and that without cartilage lesion was 99.47?0.24 two years after operation. The data showed a significant statistical difference between the two groups. The accelerating and decelerating running ability were regained in all cases without limping one year after operation. Conclusion Arthroscopic reconstruction of anterior cruciate ligament with quadrupled stranded semitendinosus tendon and suture plate is a reliable way to restore the knee function. The combined cartilage lesion has an obvious influence on the knee function.