HIV Infections ; prevention & control ; Humans

Objective To establish and verify a multiplex fluorescence quantitative PCR method for detection of CHO host cell residual DNA content and fragmentation degree, so as to provide a reliable method for safety detection of related vaccines.Methods Two sets of primers(Alu-L and Alu) and universal probe were designed for clone250 and clone49c, members of Alu sequence family of CHO cells. The UBI3 gene of capsicum was introduced as external standard gene, and corresponding primers and probe were designed. Multiplex reaction system of Alu sequence and external standard gene was established, and the residual DNA of CHO cells in samples was quantified by the multiplex fluorescence quantitative PCR. The linear range,limit of quantitation(LOQ), specificity, robustness, precision and accuracy of the method were verified. In addition, the DNA fragmentation degree of CHO cells was determined by comparing the △Ct [Ct(Alu-L)-Ct(Alu)] of the amplification curves of long and short primers of Alu sequences. Results The multiplex reaction standard curve had a good linear relationship in the range of 0. 001-0. 1 ng/μL, R~2≥ 0. 99. The addition of external standard gene had no effect on the detection ability for CHO target, and the LOQ was 0. 5 fg/μL. The DNA of human, E.coli, rats and mice had no effect on the detection, the primers targeting residual DNA of CHO cells had no specific amplification in the genomes of seven animal cell lines, and the fragmented DNA samples showed no effect on the detection results. The CVs of precision verification were all less than 15%, and the recoveries of simulated samples in normal saline and PBS containing 1% BSA were in the range of 70%-130%. The△Ct of Alu and Alu-L amplification curves increased with the degree of sample fragmentation, and the fitting curve R~2≥ 0. 99.Conclusion The multiplex fluorescence quantitative PCR detection method established in this study can rapidly and accurately quantify the residual DNA of CHO cells and determine the fragmentation degree of samples according to △Ct, which can be used for the safety detection of the related vaccines.

Liquichip technology utilizes different fluorescent coding microbead as carriers of bio-probes and flow cytometry as optical detection method,to accomplish bio-molecules reaction in suspension liquid system.This article introduces the application of liquidchip technology in the analysis of autoimmune disease,cytokine,contagious disease,endocrinopathy,neural disease,tumor marker detection and so on.With the advantages of high sensitivity,high speed,high flux,multi-analyte,high accuracy and excellent repeatability,liquichip technology has promising prospect to be widely used as advanced biomedical detection platform.

Antipsychotic Agents ; therapeutic use ; Brain ; physiopathology ; Cognition Disorders ; genetics ; Gene-Environment Interaction ; Genetic Variation ; genetics ; Humans ; Schizophrenia ; genetics ; physiopathology ; Schizophrenic Psychology ; Social Behavior ; Thinking ; physiology

Cochlear Implants ; Magnetic Resonance Imaging ; Positron-Emission Tomography

Spine metastasis stereotactic body radiation therapy delivers conformal high radiation dose to the tumor target, and steep falloff dose gradients protect adjacent normal structures, especially spinal cord. Some studies demonstrated stereotactic body radiation therapy for metastatic tumors of the spine is safe and clinically effective. This technique markedly improved local control with an excellent symptomatic response compared with conventional external beam radiation therapy techniques, and is beginning to change the treatment paradigms for metastatic spine disease both as post-operative adjuvant therapy and primary definitive local treatment. Although the technique is being increasingly used in clinical practice, the optimal fractionation schedule remains uncertain. Based on the research of radiobiologic principles, radiological physics and clinical data, multiple session stereotactic body radiation therapy has some advantage in clinic.

CD_(44) adhesion molecule is a cell surface transmembrane glycoprotein.It displays many varinat isoforms generated by alternative splicing of exons, including CD_(44s). and CD_(44v). It has been reported that CD_(44) adhesion molecule is strongly expressed on the surface of leukemic blasts in all acute myeloid leukemia subtypes.It has been found through in vitro study that CD_(44) monoclonal antibodies can inhibit proliferation of all acute myeloid leukemia cell lines and induce terminal differentiate or apoptosis of some cell lines to diverse extent. In this review the relationship between CD_(44v), and leukemia was analysed.

Expansive type of hepatocellular carcinoma (HCC) is a subtype of HCC. Correlation between the preoperative sonography and postoperative pathology was studied in 35 cases of HCC. According to the classification of nakashima, 21 cases belonged to the expansive type whereas the other 14 cases the infiltrative type. Different from the infiltrative type. the expansive type of HCC had four specific sonographic appearances: hypoechoic ring, hypoechcic halo, pedunculated and small nodular echo which had different pathological bases. Expansive tyje of HCC was not so commonly seen as infiltative type but its rate of surgical resection was mu~h higher than that of infiltratine type (JP< 0. 01). Thus, it is the kind of HCC with significant clinical importance. Preoperative ultrasonographic diagnosis help to decide the way of treatment.

HLA (human leukocyte antigen) is critical molecule relevant to immunoresponse, which is always involved in antigen presentation and determining killing activity of immune cells against tumor. The abnormal expression of HLA in lung cancer is believed to be a common phenomena of tumor biology. It has been demonstrated that HLA expression varies in various types of lung cancer, even in differential stages of one type of lung cancer. HLA expression is highly associated with genre, progression and prognosis of lung cancer. The thorough investigations of the relationship between HLA and lung cancer and of mechanism of HLA abnormality will provide useful information for studying of development of lung cancer and tumor immunotherapy.

At present clinic study and use of anti-tumour platinum drug are wide and active. The paper reviews progression of clinic study and use of anti-tumour platinum drug and expects its prospect.