Pancreatic cancer was one of common malignant tumors of the digestive tract, early diagnosis of which was difficult and surgical resection rate was low. The prognosis was poor, and its incidence increased year by year. Conventional treatment of pancreatic cancer includes surgery, radiotherapy and chemotherapy, but 90％ of the patients had lost the surgical opportunity when they see a doctor, and not sensitive to radiotherapy and chemotherapy. Relative to these traditional treatments, gene therapy is an innovative approach for the treatment of pancreatic cancer and had attracted wide attention in recent years. Many gene therapy drugs for pancreatic cancer had now entered Ⅰ / Ⅱ clinical trials. The gene therapy techniques for pancreatic cancer included antisense gene therapy, suicide gene therapy, immune gene therapy, and oncolytic virus therapy. In this article, we reviewed recent advances in gene therapy for pancreatic cancer.

Objective To investigate the effect of budesonide inhalation combined with azithromycin in treatment of children with mycoplasma pneumonia and the corresponding clinical nursing pathway.Methods From February 2010 to 2011,100 cases of children patients with mycoplasma pneumonia (all with budesonide inhalation combined with azithromycin) were randomly divided into the experimental group and the control group with 50 patients in each group.The experimental group adopted clinical nursing pathway,while the control group underwent routine care.After one or three-month follow-up,forced vital capacity (FVC),forced vital capacity 1s (FEV1),peak expiratory flow values (PEF) and other pulmonary functional indexes and clinical efficacy were compared between two groups.Results The clinical effective rate of the experimental group was 94％,higher than the control group.FVC,FEV1,PEF changes in the experimental group were better than the control group.Conclusions Budesonide inhalation combined with azithromycin in treatment of mycoplasma pneumonia in children has good effect,coupled with the clinical nursing pathway can effectively improve lung function,reduce the incidence of adverse reactions during treatment,significantly improve the prognosis,should be used widely in clinic.

Objective To compile an applicable inventory of academic diligence for middle school students. Method By means of literature analysis and in-deep interview with teachers, an academic diligence inventory was constructed and used for 393 high school students. Such methods as correlation analysis, exploratory factor analysis and reliability analysis were analyzed by SPSS11. 0 for windows XP. Results ① Five principal components of academic diligence were selected, which could be called target monitoring, time investment, task commitment, persistence and concentration. ②Internal consistency reliability and split-half reliability of total scale are 0. 9126 and 0. 9003. Reliability of each subscale are above 0. 7615. Correlation coefficient between subscales and total scale ranged from 0. 672 to 0. 820. That of among subscales ranged from 0. 248 to 0. 521. Conclusion Reliability and validity of the inventory of academic diligence for middle school students are both satisfactory enough to meet psychometric indexes.

Objective To explore the effect of cryo/cuff system at different durations on postoperative swelling and pain in patients with knee arthroscopy.Methods Ninety-two patients having undergone knee arthroscopy were divided into 3 groups: the study group(n=31),the control group(n=31)and the blank control group(n=30).The study group were managed with circulating cold compress therapy for 30 mins once every 8 hours 24 hours after operation,the control group with circulating cold compress therapy for 30 mins once every 8 hours 48 hours after operation and the blank control group with routine nursing.The degree of pains was assessed using visual analogue scale and the level of swelling using the difference in the shank circle between the diseased and healthy limbs.Results The study group and control group were both better than the blank group in swelling and pain control(P<0.05). The study group showed no difference in postoperative pains and swelling than the control group(P>0.05).Conclusion Postoperative knee arthroscopy,using cryo/cuff system can alleviate pains and swelling of joints,which is conducive for the early ambulation and promoted rehabilitation.

The duration of QRS wave,P wave and T wave of ECG can be computerized auto-detected with digital signal process and waveform recognition technology. It can diagnose 15 kinds of abnormal ECG such as ventricular presystole automatically and print out corresponding diagnosis reports. In order to verify the systems stability and creditability, we used American MIT-BIH database to test our algorithms and got a good result.

Aim To study whether or not the green tea catechins(EGCG)has physiological benefits and the underling protective mechanism.Methods The subunit protein levels of ?4、?7 of nAChR were detected by BCA protein assay,Dot Blot assay and MTT assay.Results The results showed that the green tea catechins can significantly reduce the subunit protein levels of nAChR and decrease the cell activity induced by A?1-40.Conclusions EGCG can provide neuroprotection in vitro by up-regulating nAChR sununit levels and inhibiting the neurotoxin of A?1-40.

OBJECTIVETo compare the difference among the combined method of oral administration of forlaxand acupoint embedding therapy, the simple acupoint embedding therapy and the simple oral administration of for-lax in the clinical efficacy on diabetic constipation.

METHODSOne hundred and fifty patients were randomized intoa comprehensive group, an acupoint embedding group and a forlax group, 50 cases in each one. In the acupointembedding group, the embedding therapy was applied to bilateral Tianshu (ST 25), Daheng (SP 15), Shangjuxu(ST 37) and Dachangshu (BL 25), once a week. In the forlax group, forlax (polyethylene glycol) was prescribedfor oral administration, once a day, 10 g each time. In the comprehensive group, the acupoint embedding therapyand forlax were combined and the methods were the same as the first two groups. The treatment for 4 weeks wasas one session, and 2 sessions were required in the three groups. Separately, in 4 weeks, 8 weeks of treatment and2 months after treatment, the constipation symptom scores were compared among the three groups. At the end of2 sessions of treatment, the clinical efficacy and adverse reactions were compared among the three groups. In2 months after treatment, the recurrence rate was compared among the three groups.

RESULTSThe total effectiverate was 98. 0% (49/50) in the comprehensive group, better than 86. 0% (43/50) in the acupoint embeddinggroup and 78. 0% (11/50) in the forlax group (both P<0. 01). In the 4 weeks and 8 weeks of treatment, the con-stipation symptom scores were reduced significantly as compared with those before treatment in the three groups(all P<0. 05). The results in the comprehensive group were lower than those in the other two groups (all P<0. 05). In the 4 weeks of treatment, the scores were not different significantly between the acupoint embedding group and the forlax group (P>0.05). In 8 weeks of treatment and 2 months after treatment, the scores in the acupoint embedding group were better tan those in the forlax group (all p<0.05). There were 2 cases of drug adverse reaction in the comprehensive group, 6 cases in the forlax group and 0 case in the acupoint embedding group. The recurrence rate was 8.1% (4/49) in the comprehensive group, lower than 32.6% (14/43) in the acupoint embedding group and 59.0% (23/39) in the forlax group (both P<0.01).

CONCLUSIONthe combined therapy of acupoint embedding and forlax achieves the better clinical efficacy on diabetic constipation and constipation symptom scores as compared with the simple acupoint embedding therapy and the oral administration of forlax the short-term efficacy of the simple acupoint embedding therapy is not different significantly from the simple forlax medication, but the long-term efficacy and safety are better than those of simple forlax medicaiton.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Combined Modality Therapy ; Constipation ; drug therapy ; etiology ; physiopathology ; therapy ; Defecation ; Diabetes Complications ; complications ; physiopathology ; Female ; Humans ; Laxatives ; administration & dosage ; Male ; Middle Aged ; Polyethylene Glycols ; administration & dosage ; Treatment Outcome

11?-hydroxylase deficiency is one of the main causes of congenital adrenal hyperplasia (CAH),which is caused by the mutation of CYP11B1 gene that encodes the enzyme.Researches have shown that mutations of CYP11B1 gene would result in decreased activity or inactivation of the enzyme in classical 11?- hydroxylase deficiency,and their relationship between genotype and phenotype of 11?-hydroxylase deficiency is not clear.

Objective To observe the effect of nursing intervention on children with hepatitis C receiving Kurorinone treatment.Methods 98 cases of children patients with hepatitis C admired from February 2008 to May 2010 were randomly divided into the intervention group (50 patients)and the control group(48 patients),all patients were treated with kurorinone for 3 months (one course of treatment),besides the intervention group received additional nursing.The liver function and serum HCV-RNA changes were compared between the two groups.Results Recovery of liver function in the intervention group was significantly better,negative rate of HCV-RNA in the intervention group was significantly higher than that of the control group.Conclusions Nursing intervention can improve treatment effect of kurorinone for children with hepatitis C.

Objective To investigate the clinical efficacy, the toxicity and side reactions of interventional chemoembolization with FOLFOX4 regimen through both hepatic artery and superior mesenteric artery, i.e. dual access technique, in treating primary hepatocellular carcinoma. Methods Between November 2010 and March 2013 at authors’ hospital, a total of 21 patients with advanced primary hepatocellular carcinoma (the study group) were treated with FOLFOX4 regimen by using dual access interventional technique. FOLFOX4 regimen included hepatic arterial infusion of 5-fluorouracil 400 mg/m2, hepatic arterial chemoembolization with iodipin and oxaliplatin 85 mg/m 2, intravenous administration of calcium folinate 200 mg/m2 IV on the first and second day, trans-superior mesenteric artery continuous infusion (lasting for 22 hours) of 5 -Fuorouracil 600 mg/m2 on the first and second day. During the same period other 21 patients with primary hepatocellular carcinoma were selected (used as the control group) to receive conventional hepatic arterial chemoembolization. In both groups, the treatment was repeated after 4-6 weeks. The therapeutic effect and the toxicity and side reactions were evaluated after the second treatment. Results The effective rate for the study group and the control group was 61.9% and 28.6% respectively, and the median survival time for the study group and the control group was 14.7 months and 9.4 months respectively. The differences in the effective rate and the median survival time between the two groups were statistically significant (P = 0.030 and P = 0.034). The occurrence of toxicity and side reactions, such as digestive tract reactions and the damage of liver function, in the study group were strikingly lower than those in the control group. Conclusion Through dual approach of hepatic artery and superior mesenteric artery catheterization, interventional chemoembolization with FOLFOX4 regimen is outstandingly effective for primary hepatocellular carcinoma, meanwhile, the side effects are very slight.