Antipsychotic Agents ; therapeutic use ; Brain ; physiopathology ; Cognition Disorders ; genetics ; Gene-Environment Interaction ; Genetic Variation ; genetics ; Humans ; Schizophrenia ; genetics ; physiopathology ; Schizophrenic Psychology ; Social Behavior ; Thinking ; physiology

Objective To observe the effect of several antihistamines,including epinastine,on the release of leukotriene B4(LTB4),interleukin 4(IL-4)and interleukin 5(IL-5)from spleen lymphocytes of allergic mice challenged by ovalbumin(OVA).Methods OVA-sensitized BALB/c mice were used as animal model of allergy.Spleen lymphocytes isolated from these mice were treated with epinastine(0.1,1.0,10 μmol/L),ebastine(10 μmol/L),cetirizine(10 μmol/L),and dexamethasone(1.0 μmol/L),respectively,followed by incubation with OVA.Those cells without pretreatment with antihistamines served as the control.One hour after the challenge,enzyme-linked immunosorbent assay(ELISA)was applied to measure the level of LTB4,IL-4 and IL-5 in the cell culture supernates.Results Following the incubation with OVA,the levels of LTB4,IL-4 and IL-5 increased significantly in culture supernates of control spleen lymphocytes.Epinastine significantly inhibited the release of LTB4,IL-4 and IL-5 in a dose-dependent manner.At the concentration of 10 μmol/L,the inhibitory effect of epinastine was more potent than that of ebastine and cetirizine for IL-5 and LTB4 release(all P＜0.05):for IL-4,the efrect of epinastine was similar to that of ebastine (P＞0.05)but more potent than that of cetirizine(P＜0.05).Conclusion Epinastine has a potent anti-inflammatory effect.

Objective To explore the application of thinprep cytology test thinprep cytologic test ( TCT) and the serum squa-mous cell carcinoma antigen ( SCC-Ag) measurement in the cervical lesions screening .Methods TCT and serum SCC-Ag measure-ment were performed in 58 patients with cervical lesions .Based on pathology diagnosis , specificity and sensitivity of these two screen-ing methods were calculated .Results Sensitivity and specificity of TCT detection were 75%and 65.8%, the sensitivity and specific-ity of SCC-Ag detection was 60%and 86.9%, respectively, and specificity of combined detection were 55%and 89.4%, respective-ly.There was statistical significance in TCT test and SCC-Ag test ( P <0.05 ) , the difference had statistical significance of combined detection of both with a single TCT to detect differences ( P <0.05 ) .Conclusions The combination of TCT and SCC-Ag detection can improve specificity and there was a certain clinical application value in the screening of cervical lesions .

Dust ; prevention & control ; Germany ; Humans ; Metallurgy ; Occupational Exposure ; prevention & control ; Occupational Health ; Population Surveillance ; Silicon ; adverse effects

Acute Disease ; Adult ; Aged ; Dengue ; diagnosis ; Diagnosis, Differential ; HIV Infections ; diagnosis ; HIV-1 ; immunology ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Singapore ; Young Adult

Objective To evaluate the therapeutic effeet of transurethral non-contact laser in the bladder neck stricture. Methods Q-9000 laser generator was used.The Nd-YAG laser was located to the bladder neck by laser fiber(USA).The bladder neck was cut on the point of 12 and 6 in all 39 cases. Results The maximum urinary flow was increased from (8.2?2.4)ml/s to (16.5?3.2)ml/s after operation. Conclusions Transurethral non-contact laser is effective for the treatment of bladder neck stricture.

Objective To evaluate the safety and efficacy of thoracoscopic lobectomy for pulmonary benign diseases.Methods Between July 2002 and September 2007,35 cases with pulmonary benign diseases underwent thoracoscopic lobectomy in our hospital.Of the patients,video-assisted thoracoscopic lobectomy was performed on 12 cases,and totally endoscopic lobectomy was carried out in 23.Results The operation was completed in all of the cases except in one who was converted to open surgery because of massive hemorrhage.In this series,no peri-operational death occurred,however,complications occurred in 3 cases(morbidity rate: 8.6%),including 2 cases of persistent air leak and 1 case of pneumonia.The mean duration of chest tube drainage was 3.6 days(2 to 7 days),and average hospital stay after operation was 7.7 days(2 to 14 days).Postoperative pathological diagnosis included bronchiectasis in 15 patients,pulmonary inflammatory pseudo-tumor in 6,tuberculosis in 5,fungal infection in 5,pulmonary sequestration in 2,and bronchogenic cyst in 2.Conclusions Thoracoscopic lobectomy is safe and effective for pulmonary benign disease.

The morbidity and nortality of esophageal carcinoma showed an upward trend in recent years.Neoadjuvant radiochemotherapy is the nain mode of treatment for advanced esophageal carcinoma,including neoadjuvant chemotherapy,neoadjuvant radiotherapy and neoadjuvant radiochemotherapy.However,there are still many controversies on efficacies and advantages or disadvantages of treatment mode.In this article,authors will investigate the present situations and future direction of neoadjuvant therapy for locally advanced esophageal carcinoma.

Acupuncture has become a popular alternative modality in the treatment of back pain. However, few have reported complications related to this treatment modality. This study reports a rare case of septic arthritis of the lumbar facet joints, developing after acupuncture, and its devastating effects upon the patient. A 68-year-old female developed septic arthritis of the lumbar facet joints after acupuncture for symptomatic control of lumbar spondylosis and stenosis while waiting for surgery. Subsequently, successful control of the infection required a total of 4 hospital admissions over a 2-year period, a CT guided spinal biopsy and 6 months of antibiotics.

Objective To investigate the transfection and expression of pcDNA3 1-VEGF 165 plasmid in mammalian cells. Methods pcDNA3 1-VEGF 165 plasmid was transfected into CHO cells by electroporation and the positive clones were screened by G418. The expression of VEGF 165 gene in the transfected CHO cells were detected by RT-PCR, ELISA and western blotting analysis. The biologic activities of VEGF 165 secreted by the transfected CHO cells were measured by Mile's assay and MTT method. Results G418-resistant CHO cell clones were obtained. RT-PCR, ELISA and western blotting analysis showed that there were the transcription and translation of VEGF 165 gene in anti-G418 CHO cells. The mile's assay showed that the expressed VEGF 165 could increase the vessel permeability, and MTT assay proved that it could promote the proliferation of endothelial cells. Conclusion pcDNA3 1-VEGF 165 plasmid could express VEGF 165 with biological activities in mammalian cells.