Objective:To explore the diagnostic value of combined detection of tumor markers on elderly patients with cholangiocarcinoma.Methods:Radioimmunoassay and immunoradiometricassay were adopted to detect CEA,CA19-9 and CA50 in venous serum of 35 elderly patients with cholangiocarcinoma and 31 cases of benign bile duct disease.Results:CA19-9and CA50 in serum had better sensitivity in diagnosing cholangiocarcinoma,and the rates were 80.00% and 91.48% respectively.CEA in serum had the best specificity with the rate as 87.10%.Parallel testing was superior to series testing in combined detection with the sensitivity remaining as 88.64% and the accuracy promoted.Conclusion:The combined detection of tumor markers had cooperative function in diagnosing the elderly patients with chola ngiocarcinoma,with the accuracy promoted,and the rates of missed diagnosis and misdiagnosis lowered.

Objective:To discuss the effect of bone marrow stem cell mobilization on density and function of macrophages in deep vein thrombosis.Methods:Adult SD rats were divided randomly into sham operation group,thrombus group and rhG-CSF group.Analyzed the density change of macrophages in thrombus on the 7th and 14th day after operation and observed the function of macrophages in thrombus by electron microscope.Results:Density of macrophages in thrombus significantly increased in rhG-CSF group,compared with that in thrombus group(P

Objective: To discuss the effect of bone marrow mobilization on chemotaxis of aggregation of macrophages in venous thrombosis. Methods:SD adult rats were divided randomly into normal group,sham operation group,thrombus group and rhG-CSF group.Peripheral blood was harvested at 30min before operation and on the 7th and 14th day after operation.Level of MCP-3 in peripheral blood was detected by ELISA.Inferior vena cava specimens of thromus group and rhG-CSF group were collected at postoperative 7th and 14th day. With the application of RT-PCR,expression of MCP-3mRNA in thrombus was detected.Results:The level of MCP-3 in peripheral blood of rhG-CSF group was significantly higher than that before operation and also higher than the levels of MCP-3 in peripheral blood of sham operation group and thrombus group on the 14th day after operation(P<0.05),and the difference was significant.On the 7th and 14th day after operation,compared with thrombus group,the expression of MCP-3 mRNA in rhG-CSF group remarkablely increased,and the difference Was significant beween these two groups(P<0.01).Conclusion:Brone marrow cell mobilization enhances the chemotaxis of MCP-3 on macrophages.

Proton magnetic resonance spectroscopy (HMRS) is the only way to study noninvasively the metabolic and biochemical signature of the human tissues and organs.It can also quantify the metabolites within the volume of interest.The metabolic information about the lesions provided by the HMRS can greatly improve the diagnostic,grading accuracy,treatment planning such as target delineation and posttreatment follow-up of the glioma.

Objective:To discuss the effect of bone marrow on chemotaxis of aggregation of macrophages in venous thrombosis.Methods:SD adult rats were divided randomly into normal group,sham operation group,thrombus group and rhG-CSF group.Peripheral blood was harvested at 30min before operation and on the 7th and 14th day after operation.Level of MCP-3 in peripheral blood was detected with ELISA.Inferior vena cava specimens of thromus group and rhG-CSF group were collected at postoperative 7th and 14th day.With the application of RT-PCR,expression of MCP-3mRNA in thrombus was detected.Results:The level of MCP-3 in peripheral blood of rhG-CSF group was significantly higher than that before operation and also higher than the levels of MCP-3 in peripheral blood of sham operation group and thrombus group on the 14th day after operation,(P

Objective:To observe the effect of thyroid hormones on 11?-hydroxysteroid dehydrogenase type 1(11?-HSD1) in newborn rats' hippocampus. Methods: Hypothyroid model was induced with propylthiouracil(PTU, 50 mg/d) in SD rats in the last week of pregnancy and was confirmed by measuring the levels of thyroid hormones and thyrotropin(TSH) in the newborn rats' blood. Western blot was used to determine the expression of 11?-HSD1 in hippocampus in newborn rats. Effects of triiodothyronine(T 3) and synthetic glucocorticoid dexamethasone(Dex) on expression of 11?-HSD1 in primarily- cultured hippocampus were studied with thin layer chromatography(TLC) and Western blot. Results: Thyroid hormone levels decreased and TSH level increased in the newborn rats' blood. The expression of 11?-HSD1 decreased by 41.6% in the hippocampus of newborn rats born to the mothers treated with PTU. T 3(10 -9 ,10 -8 ,10 -7 mol/L) and Dex(10 -7 mol/L) up-regulated the activity of 11?-HSD1 by 29.4%,45.6%,60.9% and 39.8%,respectively. T 3(10 -9 ,10 -8 ,10 -7 mol/L) and Dex(10 -7 mol/L) increased the expression of 11?-HSD1 protein by 26.0%,43.2%,64.9% and 41.1%,respectively.Synergistic effect of T 3 and DEX was observed in terms of regulating 11?-HSD1: the expression of 11?-HSD1 increased by 123.3% in the hippocampal neurons exposed to both Dex(10 -7 mol/L) and T 3 (10 -7 mol/L) and the activity increased by 114.1%. Conclusion:Thyroid hormone can up-regulate the expression of 11?-HSD1 in the hippocampus during brain maturation either by itself or synergistically with glucocorticoid, thereby enhancing the action of glucocorticoids on the brain.

0.05).There was significant difference in expression of CerbB2 in breast cancer with different histology class and lymphatic metastasisi numbe(rP

AIM:To evaluate the economic effectiveness of different therapeutic schemes for benign prostatic hypertrophy METHODS:175 patients with benign prostatic hypertrophy were divided into 4 groups:A,B,C and D They received different drugs,A:tamsulosin(34),B:prostat(45),C:tedenan(46) and D:qiangliexin(50) RESULTS:The costs of A,B,C,D schemes were RMB 492 80,499 52,526 40 and 664 16 yuans,respectively,and the effective ratios were 85 3%,82 2%,80 4% and 80 0%,respectively CONCLUSION:According to the evaluation with pharmacoeconomic cost-effectiveness analysis,A is the best scheme

Objective To evaluate the clinical application of autologous fat and acellular dermal matrix (ADM ) in vocal fold medialization .Methods A total of 16 New Zealand white rabbits of both sexes were divided into two study groups (8 rabbits in each group) ,after unilateral recurrent laryngeal nerve section ,autologous fat or ADM were injected into paralyzed vocal folds .Laryngoscopic exams were performed 2 ,4 ,8 ,and 12 weeks after implanta‐tion ;then larynx specimens were sampled and serially sectioned in axial plane from the false vocal fold to the sub ‐glottis .After section was stained with HE ,histologic behavior of the graft were evaluated by optical microscope , volume of remaining material were calculated by autoCAD software .Results The remaining volume of autologous fat and ADM reduced gradually ,there were no statistically significant differences between the 2 groups .In 2 weeks , fat cells survival was mass distribution ,as its necrosis and absorption ,intraimplant resulted in a plurality of cavitati‐on ,encapsulated by fibrous connective ,mixed with a little survival fat cells .In 12 weeks ,obvious capsule formation surrounded lipoma composed of fiber connective tissue .In the ADM group ,2 weeks after injection ,intra - implant fibroblast activity and neovascularization was noted ,later fibroblast infiltration increased gradually .In 12 weeks ,the implantation maintaun the basic outline and have compact organizational structure ,but not forming obvious bounda‐ries with the surrounding tissue .Conclusion Comparing degree of absorption ,there were no statistically significant differences between autologous fat and ADM in the study ,but histological outcome of ADM is closer to the original organization .In future ,ADM optimization or combination with other materials ,will have a larger development and clinical application value .