The distribution of factor H of serum phenotypes were studied using the ultra-thinpolyacrylamide gel isoelectric focusing (PAGIEF) followed by Immunoblotting technique in 203Chinese Han population in Liaoning area. The gene frequencies were as follows: HFA0. 4828,HFB0.5172. The observed numbers of the phenotype were in agreement with the expected numbers underthe Hardy-Weinberg equilibrium. The gene frequencies among Chinese and other population werecompared.

Objective To evaluate the accuracy of preoperative diagnosis on the anatomy of coronary artery of patients with arterial switch operation(ASO) using echocardiography, to conclude the diagnostic methods. Methods From March 2007 to March 2008,50 patients were performed ASO after the diagnosis with eehoeardiography. Prior to the operation, evaluation was conducted to all of anatomical pattern of the coronary artery,and the comparison was made to the observation in the operation. There were 23 patients with complete transposition of great artery(TGA), 20 patients with double outlet of right ventricle of abnormal great artery(DORV), 2 patients with corrected transposition of the great arteries(CTGA), 1 patients with double outlet of left ventricle of abnormal great artery (DOLV), 2 patients with TGA complicated with total anomalous pulmonary venous connection (TGA + TAPVC), 2 patients with TGA complicated with pulmonary valve stenosis(TGA + PS). Results Among 50 patents,there were 40 patients with normal coronary artery,and 10 patients with abnormal coronary arteries. Except for 2 patients failing to being successfully diagnosed due to bad condition of image,48 patients could be revealed the origin and the direction of the major coronary arteries, the visibility proportion was 96.0%. Compared to the observation in the operation,the total echocardiography accuracy for 48 patients was 95.8%. Among the 40 patients with normal coronary arteries,39 patients were diagnosed accurately except 1 patient failed to be showed clearly by echo. In the 10 patients with abnormal coronary arteries,4 patients with single abnormal coronary artery, 2 patients with 2 coronary artery originating from the same coronary sinus, 2 patients with left anterior descending coronary artery originating from right coronary artery, 2 patients with 2 coronary artery originating from two adjacent coronary sinus. One patient failed to be diagnosed by echo for the bad image,2 patients was misdiagnosed by echo, the accuracy of echo was 77.8%. Conclusions Through multi-section scanning,echocardiography can accurately diagnose anatomical pattern of coronary artery for most of ASO,thus providing comprehensive and accurate diagnosis information for ASO.

Objective To investigate the clinical effects and operation key-points of interphalangeal joint replacement arthroplasty for the treatment of traumatic arthritis.Methods From March 2005 to December 2010,5 patients with post-traumatic arthritis undergoing interphalangeal joint replacement arthroplasty in our hospital were selected.The operation indications,treatment of intraoperative flexor and extensor tendon lesion and postoperative rehabilitation were evaluated.Results Operative incisions of 5 cases were primary healing in class I grade A.Stitches were taken out 2 weeks after the operation.Patients were followed up for 1.5 years.The range of interphalangeal joint motion was 0 to 90 (extension/flexion) with no pain at end of follow-up.The hand function was excellent in 3 cases and good in 2 cases according to total active motion (TAM)hand function evaluation.Conclusions Interphalangeal joint replacement arthroplasty is an effective treatment for traumatic arthritis.Preoperative indications evaluation and postoperative rehabilitation should be focused on the treatment.

Dry eye is one of the common ocular surface diseases in the world and in our country. This article investigated the prophylaxis and treatment for dry eye according to the TCM concept of preventing possible disease, emphasized preventing disease by health preserving, improving environment, rational use of drugs, reasonable diet. The cases with dry eye symptom andocular surface damage should be treated on the principle of treating disease in the early stage, preventing the disease progression at the onset of disease. It emphasized the importance of internal care and external nursing.

Objective To investigate the efficacy of normative ankle balance training on chronic ankle instability (CAI).Methods Ninety patients were randomized into control group (17 males,28 females;13-26 years,mean 19.6 years) and experiment group (18 males,27 females;14-27 years,mean 20.3 years) according to the random number table.There were 19 left ankles and 26 right ankles injured in control group,and 19 left ankles and 26 right ankles injured in experiment group.Patients in control group only completed routine muscle strength training.Besides the routine muscle strength training,balance training (rubber band,spongy cushion,balance pedal,rubber balance ball and jump ball) were added in experiment group.The two groups were compared with respect to foot and ankle disability index (FADI),anterior,posterior-median and 45° to posterolateral direction reach distance of star excursion balance test (SEBT) and visual analogue score (VAS).Results After training,comparison of experiment and control groups showed significant differences in FADI (35.16 ± 3.23 and 22.33 ± 1.36),SEBT distance in posterior median direction [(78.59 ± 7.22) ％ and (71.89 ±7.11)％],SEBT distance in 45° to posterolateral direction [(75.79 ± 8.27)％ and (68.95 ±8.03) ％] and VAS [(3.01 ± 0.91) scores and (3.42 ± 1.02) scores] (P ＜ 0.05).For intragroup comparison,FADI,SEBT and VAS in experimental group showed significant differences before and after training (P ＜ 0.05),but only FADI in control group differed significantly before and after training (P ＜0.05).Conclusion Normative balance training can improve CAI patients' self-feelings of dynamic balance and instability with satisfactory therapeutic efficacy,and is worthy to be popularized.

Objective To explore the value of echocardiography on diagnosis and differential diagnosis of aortico-left ventricular tunnel(AOLVT).Methods Echocardiographic features of 9 patients with AOLVT were reviewed and analyzed.The diagnosis methodology was summarized;the reasons for missed diagnosis and misdiagnosis by echocardiography were analyzed.Results Among 9 patients with AOLVT,one was diagnosed through CT examination prior to the operation and died from the cardiac arrest.The others all received the surgical operation and were diagnosed as AOLVT.Five patients were accurately diagnosed by echocardiography prior to the operation,4 patients were misdiagnosed,in which 3 patients were diagnosed as aortic valve regurgitation,one patient was diagnosed as aortic dissecting aneurysm.The diameter of left ventricle(LV)decreased and LV ejection fraction increased distinctively for 7 operated patients(P＜0.001)in six months after operation.Only one operated patient failed to recover from the LV dysfunction and died of aortic valve perivalvular leak in two monthes.Conclusions The diagnosis of the AOLVT mainly depends on the echocardiography.By enhancing the understanding on such disease and investigating on the structure and hemodynamics will be contributive to the accurate diagnosis of echocardiography.

Fibroblast growth factor receptor 3(FGFR3)plays important roles in cell proliferation,diffe rentiation,and angiogenesis.Recent studies have demonstrated that FGFR3 is associated with progression of breast cancer and has effects in endocrine therapy resistance breast cancer.It has also been showed that FGFR3 is correlated with breast cancer prognosis.

Objective To explore the water quality of the product water from the centralized water supply system in Hunan, 2006. Methods The sanitary quality of the product water samples collected from 28 centralized water supply system in cities and 39 centralized water supply system in counties in Hunan, 2006, which distributed among the area of Xiangjiang River, Zishui River, Yuanjiang River and Lishui River, were determined and evaluated according to the Standards for Drinking Water Quality (GB5749—2006). Results The eligible rate of the water sample was 70.1%. Among 74 water quality indexes, 12 indexes (such as antimony,aluminum,pH value,manganese and so on) exceeded the standard limits in degrees in some centralized water supply systems. Most of the routine indices of water quality were unqualified and the reference indices were qualified. The quality of some water samples presented statistical difference in some indices (such as aluminum, sulphate, arsenic, et al) among 4 different river areas in Hunan province (P

Objective To study effect of Shikonin on human cervical cancer Hela cell growth suppression in vitro and its mechanism. Methods MTT assay was used to examine the growth inhibition of Shikonin in Hela cells.And then, the measurement of both ROS Levels and Mitochondrial Membrane Potential (ΔΨm ) were performed to clarify the mechanism of antitumor in Hela cells by Shikonin.Results Shikonin significantly inhibited the growth of Hela cells in a concentration-dependent manner.Shikonin increased generation of en-dogenous reactive oxygen species ( ROS) and decreased mitochondrial membrane potential.Furthermore, anti-oxidants N-acetylcysteine ( NAC) could significantly reduce the antitumor activity of SK in Hela cells.Conclusion These results suggest that mitochondrial aerobic respiration shift and endogenous ROS augmentation contribute to the action of Shikonin against Hela cells.

Objective To study whether post-operative radiotherapy is necessary for patients with early breast cancer after radical mastectomy. Methods In 1998, 270 early breast cancer patients with 0 -3 pathologically confirmed positive axillary lymph nodes after radical mastectomy were retrospectively ana-lyzed. There were 156 patients with negative lymph node and 114 with 1 -3 positive lymph nodes. The prog-nostic index (PI) was defined as the sum of scores of the tumor size, number of positive axillary lymph nodes, receptor status, surgical margin status, lymphatic thrombi status, pathological grading and age. The PI≥ 4 was considered as high-risk, and PI <4 as the low-risk. Numerical variables were compared using t test and categorical variables were compared using chi-square test. Kaplan-Meier method was used to calcu-late the survival rates, and the Log-rank test was used for the comparison of the survival curves between dif-ferent groups. Results Of the patients with lymph node negative and 1 - 3 positive, the survival rates were 75.0% and 63.2% (χ~2 = 4.40 ,P =0.036), respectively. The corresponding disease-free survival rate, lo-cal recurrence rate, distant metastasis rate were 71.2% and 9.6% (χ~2 = 3.90, P = 0.048), 7.7% and 16.7%(χ~2 =5.22,P=0.022),12.8% and 21.1%(χ~2=3.27,P=0.070), respectively. The mean dis-ease-free survival time of the two groups was 97.03 ± 2.53 months and 87.01 ± 3.80 months, respectively. In the high-risk group, the 10-year survival rates of patients with and without radiotherapy were 72% and 56% (χ~2 = 4.07, P = 0.044), the local recurrence rates were 5% and 24% (χ~2= 11.16, P = 0. 001), and the distant metastasis rates were 16% and 26% (χ~2= 2.18 ,P = 0. 140). In the low-risk group, the survival rate of patients with and without radiotherapy were 81% and 71% (χ~2 = 1.57 ,P = 0.210), the local recur-rence rates were both 11% (χ~2=0.01 ,P=0.975), and the distant metastasis rates were both 13% (χ~2 = 0.00,P = 1. 000). Conclusions Early breast cancer patients with 1 -3 positive axiilary lymph nodes should receive post-operative radiotherapy after radical mastectomy. The prognostic index may decrease the chance of unnecessary radiation by distinguishing the patients under low risk of recurrence from those under high risk.