Objective To study the effect and mechanism of a selective aldosterone receptor blocker, eplerenone (EP), on renovascular hypertensive rats. Method Forty-eight Wistar rats were divided into six groups: sham operation group, control model group, early EP group, late EP group, early losartan (LO) group and late LO group. Systolic blood pressure (SBP) and urinary protein excretion (UPE) were measured before and every two weeks after operation. Histopathology and endothelial cell nitric oxide synthase (ecNOS) gene expression were examined in heart, aorta and kidney. Results SBP obviously increased in control model group as compared to sham group at week 10(132. 1 ?2.4 vs. 115.0?0.6 mmHg, P = 0. 0191), while early treatment with LO or EP could decrease SBP significantly as compared to control group. The control group developed significant UPE [ (21. 7 ? 1.9) mg/d] compared with the sham group [(13. 4?0. 8) mg/d] (P

Objective To explore the effect of scarring-moxibustion on Peyer’s patch and T cells subsets of intraepithelial lymphocyte of tumor-bearing mice with cyclophosphamide. Method The mucosal immune deficiency model was made by in site inoculating C-26 colon carcinoma with repeated intragastric administration of cyclophosphamide. The mice were treated with scarring- moxibustion for seventeen days after inoculation. The area of Peyer’s patch were measured, and T cells subsets of Lamina propria lymphocyte (LPL) were separated and detected by immunofluorescence test and flow cytometer. Result Tumor weight of tumor-bearing group was higher than cyclophosphamide group significantly (P

Objective To design and implement the implantable part of cochlear implants, i.e. multi-channel implantable neural stimulator. Methods Based on programmable logic device (PLD), the design of hardware circuit utilized hardware description language through PDM decoding and ETC controlling precept. Results The multi-channel implantable neural stimulator could provide as more as eight outputs of the channel, and the resolution of the outputs were high. Conclusion The miniature stimulator which is portable and efficient, contains perfect error correcting system, and the stimulating waveforms produced by the stimulator accord with the charge balance, current limit and charge limit.

Objective To explore the expression of merlin and cyclins and the nucleocytoplasmic shuttling of merlin in abiogenous vestibular schwannoma tissues. Methods Four paraffin imbedding vestibular Schwannoma samples were included in the study.The cyclins and merlin were analyzed by immunofluorescence at the same time,and the expression and location were synchronously observed with confocal laser microscopy(CLM) in a single schwannoma cell. Results Cyclins and merlin were perspicuously and synchronously presented in a single cell in vestibular schwannoma tissues.In early G1 phase of the tumor cell,merlin was mainly stained in the nucleus,followed by perinucleus and cytoplasm.In G1/S phase,merlin was concentrated in the perinucleus,less in the nucleus and cytoplasm.Merlin staining showed uniform distribution in the nucleus in G2/M phase,while was absent from the nucleus and concentrated in the perinucleus in late G2/M phase. Conclusion The expression of merlin and various kinds of cyclins can be synchronously presented in abiogenous vestibular schwannoma cells with immunofluorescence and CLM.Merlin is shuttling among the nucleus,cytoplasm and perinucleus along with the progression of cell cycle.

To study an improved translabyrithine approach, twenty adult cadaver heads (40 sides) fixed with formalin were dissected by mimicking translabyrithine method under light microscope ,and measurements were performed . The results showed that the rate of meeting with mastoid emissary was 33/40(82. 50%). The distance between the middle part of sigrnoid and the vertical part of facial nerve was 7. 22 ?1.95 mm. The width of the middle part of sigmoid was 10. 5 ? 0. 79mm, the left side was 8. 89 ? 1. 03mm, and the right side was 9. 88 ?2.14mm. The height of jugular bulb was 8. 08?3. 46mm,the left side was 6. 78 ? 3. 17mm,and the right side was 9. 07?3. 04mm. The distance between the highest point of jugular bulb and the base of internal acoustic meatus was 4. 54 ? 2. 81 mm, the left side was 5. 93?3. 12mm, and the right side was 2. 65?1. 87 mm. The distance between the inferior margin of internal acoustic meatus aperture and superrior petrosal sinus was 5. 09?1. 29mm . The diameter of superrior petrosal sinus was 1. 43?0. 08 mm. There were two types of superrior petrosal sinus entering to the sigmoid (or transverse sinus). The angle between superrior petrosal sinus and sigmoid was 52. 17 ?6. 93?. The results suggest that the improved translabyrithine approach can give enough vision for cerebellopontine angle operation.

Objective To investigate the therapeutic effect of ginkgo-damole combined with prostaglandin E1 and explore its influences on clinical evaluation and hemorheology paramaters in treatment of diabetic patients with peripheral arterial disease(PAD). Methods 64 patients were randomly divided into two groups: the therapeutic group was given conventional therapy adding ginkgo-damole combined with prostaglandin E1, while the control group was given ginkgo-damole only. To compare ankle-brachial index(ABI) and hemorheology changes before and after the use of ginkgo-damole combined with prostaglandin E1 in diabetic patients with PAD. Result The total effective rate was 91.2% in therapeutic group, and the significant efficiency in therapeutic group was higher than that in control group ( 67.6% vs 36.7% ) ( P ＜ 0. 05 ). Compared with before the treatment , ABI increased significantly and hemorheological parameters improved significantly in two groups after the treatment( P ＜ 0. 05 or P ＜ 0. 01 ), and the difference were more significant in therapeutic group than in control group( P ＜ 0. 05 or P ＜ 0. 01 ). Conclusion Ginkgo-damole combined with prostaglandin E1 was effective and safe in treatment of diabetic patients with PAD.

Continuous blood purification therapy, one of the most popular multiple organ protection and life support technology, has been widely used in the treatment of critical illness.After the initial introduction of the concept of dialytrauma or CRRT trauma in 2012, the related complications are getting more and more attention.The loss of heat caused by CBP may lead to hypothermia.Howev-er, to critically ill patients, controversy still reigns on the influence of this issue.There is no guideline developed through clinical prac-tice to define such issue.This article reviews the methods as well as results of related clinical studies to discuss how to manage the hy-pothermia during CBP.

Objectives To analyze the mechanisms and implications involved in the generation of platelet associated antibodies in cancer patients.Methods HLA IgG antibody from 116 cancer patients who had received repeated platelet transfusion (more than 3 times) was screened by ELISA and the GTI QUICKSCREEN kit. The positive samples from the initial screening were confirmed for HLA and HPA antibodies using the PAKPLUS reagent. Patients with antibodies were observed for symptoms and clinical effectiveness of platelet transfusions. Results A total of 44 cases of HLA IgG antibody were screened out of 116 samples, with an antibody rate of 37.93%. Among those 44 samples, 18 were confirmed to contain anti-HLA-Ⅰantibody, 8 with anti-HPA antibody, 10 with both anti-HPA and anti-HLA antibodies, and 8 were not identified. The occurrence of antibodies was positively correlated to the times of platelet transfusions and correlated to the improvement of clinical symptoms (P

In order to verify the dependability of 3D data, twenty-four temporal bones of adult head specimen fixed with formaldehyde were scanned by multi-slice helical CT. The technique of shade surface display and volume rendering were used in reconstruction. The 3D data of the surface structure of temporal bone were compared with anatomical specimens. The result showed that the new type CT has more rapid speed in 3D reconstruction and clearer imaging. The technique of shade surface display and volume rendering can give very clear structure of temporal bone. The 3D data makes no difference with anatomical measurement (no statistic difference). It is suggested that the 3d data can instruct the clinic directly.

Objective To investigate morphosis of the epiphyseal plate and the expression of vascular endothelial growth factor (VEGF) in the epiphyseal plate during healing of injured epiphysis.Methods Thirty SD rats aged 4-5 weeks were used to make models of proximal tibia epiphysis injury.These models were equally divided into 5 groups in random,with 6 rats in each group,and these groups of rats were killed separately at 2,4,6,10,21 days postoperatively to harvest the proximal tibia epiphysis,in order to explore the morphosis of the epiphyseal plate and VEGF expression pattern in the epiphyseal plate at different healing phase.The structure of the epiphyseal plate was evaluated by measuring the length of the limb and radiographic examination of the limb.The ratio of length of injured tibia and that of the other side was used to describe the tibia length discrepancy.The micro structure of the epiphyseal plate and situation of the bone bridge in the epiphyseal plate were measured by using Micro CT.By using HE stain and VEGF immunohistochemistry,the changing of chondrocyte and VEGF expression pattern in the epiphyseal plate were observed at different healing phase.Results The limb discrepancy appeared at 4 days postoperatively,became biggest at 10 days postoperatively,and tended to decrease at 21 days postoperatively.Micro CT demonstrated the fibrous bone tissue formation in the epiphyseal plate at about 6 days postoperatively,which became bone bridge finally.HE stain showed chondrocyte in the rest zone had the tendency to aggregate after injury,and the highly ordered structure of chondrocyte in the epiphyseal plate no longer existed,chondrocyte differentiation was accelerated.VEGF immunohistochemistry stain showed the high reaction of VEGF in the epiphyseal plate appeared after surgery,and the positive zone of VEGF expanded through the physis with time,which finally led to angiogenesis and ossification.Conclusion A serial alteration of morphosis of the epiphyseal plate occurred during the healing process.Fibrous bone tissue formation in the epiphyseaL plate could be observed in the early and mid-term of the healing process.With progress of healing,VEGF expression zone gradually expanded across the epiphyseal plate,which is related to the formation of bone bridge.