As highly active anti-retroviral therapy continues, large numbers of drug-resistant strains appeared in human immunodeficiency virus ( HIV) infectors, which always leads to a decline of treatment results or even a treatment failure.The genotypic resistance analysis technique of the bioinformatics is a powerful tool to forecast the HIV resistance and also determines the success or failure of antiviral therapy.This review will be focus on the advantages and disadvantages, influence factor of the genotype resistance prediction and improve measure of the primary HIV drug resistance genotype interpretation system, depending on the principle and characteristics of the genotypic drug resistance analysis as the breakthrough point, in order to provide guidance for reasonable application of different genotypes drug resistance analysis system in China.

Objective To observe the clinical effects of Jianpi Yishen granule in the relief for toxic and negative effects of chemotherapy in treating cancer.Methods Five hundred and twenty eight cases were randomized into two groups:the treatment group in which Jianpi Yishen granule and chemotherapy were used and the control group in which chemotherapy only was given.Total two cycles were performed for two groups.The effective rate,the changes of Karnofsky scores and immunity,the adverse reaction to chemotherapy in the two groups were observed before and after the treatment.Results The effective rate (CR+PR) in the treatment group and the control group was 61.4% and 60.5% respectively,and the improvement in Karnofsky scores in the fomer was higher than in the latter (P

Objective To detect the expressions of p53, vascular endothelial growth factor (VEGF) and microvessel density (MVD) in breast cancer and tumor adjacent tissues to explore their relationship and roles in the pathogenesis of breat cancer. Methods The expression levels of p53,VEGF and MVD were assessed in 110 cases of breast cancer and tumor adjacent tissues with SP immunohistochemical method. Results The positive expression rate of VEGF and p53 was 90.0%(99/110) and 42.7%(47/110) in the breast cancer tissues, and 35.5%(39/110) and 4.5%(5/110) in the tumor adjacent tissues, respectively, which had highly significant difference between two groups (P

Objective To investigate the expression of inducible nitric oxide synthase(iNOS) in the cochlear stria vascularis(SV) of guinea pigs after streptomycin(SM).Methods Auditory brainstem response(ABR) was used as measurement index,and combining with transmission electron microscope(TEM),immunohistochemical staining and image quantitative analysis technique, to detect iNOS expression in the cochlea stria vascularis of guinea pigs and the change of morphology after SM.Results 10 days after adminitration, ABR threshold of SM group increased more significantly than that of the control group(P

Objective:To explore the multi-dimensional method of medical equipment management based on comprehensive index assessment, and improve quality of medical equipment management. Methods: This essay proposed a comprehensive-index-analysis medical equipment management approach within hospital theoretic work and medical equipment management. Equipment management and project application in each clinical department were treated as evaluation criteria which conducted a multi-equality index for medical equipment operation management, using the pattern of quantitative substitute qualitative method.Results: The new method helps to quantize ability of clinical department, equipment utility and management. It refers to scientific equipment management data after procurement, which reflects differences between each department and equipments. Moreover, it helps to prevent blind procurement effectively by ranking department earning capacity.Conclusion: The new method is benefit for equipments’ evaluation and to provide reference for managers in decision-making.

OBJECTIVETo observe the effects of bloodletting pricking, cupping and surrounding acupuncture on blood inflammation-related indices in patients with acute herpes zoster (HZ), and to explore the mechanism of pain control and treatment.

METHODSA total of 60 patients were randomly divided into an observation group and a control group, 30 cases in each one. In the observation group, the patients were treated with bloodletting pricking at herpes, followed by cupping treatment; also the surrounding acupuncture was performed at injured skin. The treatment was given once a day and once every other day after the first 3 days; totally one-week treatment was given. In the control group, the patients were treated with intravenous drip of acyclovir and oral administration of vitamin B1 and B12, once a day for total one week. The visual analogue scale (VAS) and percentages of neutrophil, lymphocyte in peripheral and local blood were observed before and after treatment in the two groups.

RESULTSAfter treatment, the score of VAS was significantly reduced in both groups (both P < 0.05); compared with the control group, the score of VAS and the time of pain relieve were significantly improved in the observation group (P < 0.01, P < 0.05). Compared before treatment, the percentages of lymphocyte in peripheral and local blood were reduced after treatment (both P < 0.05) and the percentages of neutrophil in local blood were increased (both P < 0.05). The lymphocyte in local blood was also reduced after treatment in the control group (P < 0.05); compared with peripheral blood in the observation group and local blood in the control group, the percentages of lymphocyte in local blood were reduced (both P < 0.05).

CONCLUSIONThe efficacy of bloodletting pricking, cupping and surrounding acupuncture on acute herpes zoster is positive, and it can significantly lower the number of lymphocytes in the local blood and increase the number of neutrophil, which is likely to be one of the anti-virus mechanisms.

Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Bloodletting ; Combined Modality Therapy ; Female ; Herpes Zoster ; blood ; immunology ; therapy ; Humans ; Male ; Middle Aged ; Neutrophils ; immunology ; Pain Management ; Young Adult

Objective To review the safety and efficacy of fast track surgery in gastrectomy for gastric cancer.Methods The computer retrieved databases, including Pubmed, Medline, Cochrane library and Web of science, to collect randomized controlled trials (RCT) or controlled clinical trials (CCTS) on FTS was used in gastrectomy for gastric cancer between January 1994 and march 2014, and manual retrieval in Google.Using RevMan5.0 software analysis data that extract from collect literature.Results A total of five RCTs and two CCTs, involving 636 patients,were included, there were 309 cases in experimental group (FTS group) and 327 cases in control group.Meta-analysis showed: the FTS group had earlier postoperative flatus [WMD =-18.74, 95％ CI (-34.31,-3.17), P ＜ 0.05], shorter postoperative hospital stay [WMD =-2.46, 95％ CI (-3.75,-1.17), P=0.000 2], and lower hospital charge [SMD =-0.67, 95％ CI (-1.00,-0.34), P ＜ 0.000 1].However, there were no statistically significant differences in operation time, intraoperative blood loss, the number of retrieved lymph node intraoperative, the time of catheter removal and postoperative complication rate (P ＞ 0.05).Conclusion FTS in gastrectomy for gastric cancer can promote postoperative bowel function recovery, decrease postoperative hospital stay and reduce hospital charge.

Acupuncture Therapy ; Aged ; Female ; Humans ; Male ; Middle Aged ; Pterygium ; therapy ; Yin Deficiency ; therapy

Objective To construct replication deficient recombinant adenovirus of human monocyte chemoattractant protein-1(MCP-1) by homologous recombination.Methods The cDNA of MCP-1 gene was obtained from human liver tissue by using RT-PCR,and was subcloned into a transfer plasmid pAdTrack-CMV.The linearized recombinant transfer plasmid pAdTrack-CMV-MCP-1 was co-transformed with backbone vector pAdEasy-1 into bacteria BJ5183 for recombinant adenoviral plasmid.The recombinant adenoviral plasmid was linearized and then transfected into HEK293 packing cells to produce virus particles.The recombinant adenovirus was detected by using PCR.Results The recombinant adenoviral plasmid was successfully established and confirmed by restriction endonuclease digestion.The expression of green fluorescent protein(GFP) was observed on the 5th day after transfection.The fragment of MCP1 gene was amplified by PCR.Conclusion The achievement of recombinant adenoviral plasmid and recombinant adenovirus of MCP-1 lay a foundation for further investigation of the function and application of MCP-1.

Objective To evaluate thoracic endovascular aortic repair for type B aortic dissection.Methods Chnical data were reviewed on 126 cases with type B aortic dissection undergoing endovascular aortic repair in our hospital from January 2006 to April 2011.There were 86 male patients and 40 female patients,age from 32 to 82 years.The stent- grafts were introduced via femoral arteriotomy.Stent-grafts were implanted to blockade tears of aortic dissection under the guidance of DSA.Postoperatively patients were followed-up by angiography and imiging for endoleak,stent migration,and fracture of stent-graft.Results In all cases,the aortic dissection tears were closed,true lumens were opened,and organ function was restored.There were not complications such as internal hemorrhage due to trauma or stent displacement on CTA from 3 to 63 months after endovascular therapy.Procedure was successful in all 126 cases,157 stents were released,2 cases died in the perioperative period,1 case died during the followed-up.A breach was found at the end of the stent in 12 cases,endovascular aortic repair redone successfully with a retrograde type A dissection found during follow-up.Conclusions Endovascular aortic repair is safe and effective for patients with Stanford type B aortic dissection with a favorable outcomes.