1.Psychological stress among nurses in Department of Emergency of Beijing Jishuitan Hospital
Xiaojie LIU ; Yang GOU ; Yan HAO
Journal of Preventive Medicine 2022;34(12):1279-1282
Objective:
To investigate the psychological stress among nurses in the Department of Emergency, Beijing Jishuitan Hospital, so as to provide the evidence for formulating targeted psychological health interventions.
Methods:
All nurses from Department of Emergency, Beijing Jishuitan Hospital were enrolled, and participants' demographics, professional title, working duration, family support and participation in public health emergency responses were collected using questionnaire surveys. The development of psychological stress during the latest one week was evaluated using the Symptom Checklist-90 (SCL-90) scale, and factors affecting psychological stress were identified.
Results:
A total of 121 valid questionnaires were recovered, and the participants included 6 men (4.96%) and 115 women (95.04%) and had a mean age of (41.49±4.61) years; and mean service length of (12.14±2.61) years. There were 83 participants that were married (68.60%), 71 with a bachelor degree (58.68%), 60 with a medium-grade professional title (49.59%). The mean score of psychological stress symptoms was 213.43±18.58, which was significantly higher than national norm (124.57±14.31; t=12.194, P<0.001). The detection of psychological stress was 31.40% among the participants, and the presence of psychological stress correlated with age of 40 years and older (χ2=4.826, P=0.028), no family support (χ2=40.420, P<0.001), and no participation in public health emergency responses (χ2=33.767, P<0.001).
Conclusions
A high degree of psychological stress is detected among nurses in Department of Emergency of Beijing Jishuitan Hospital, and age, family support and emergency response capability are factors affecting psychological stress.
3.An Exploration into Offering Functional Experiment Course in Medical College
Hong SUN ; Kexin DU ; Hao HU ; Wei GOU
Chinese Journal of Medical Education Research 2003;0(04):-
Medical college of Xi`an Jiaotong university offered the functional experiment course in 1997. The courseincludes the basic knowledge of medical scientific research, the experimental design and the implementation of theexperiment, totally 24 hours. The course determines the key role of students in teaching, cultivates their ability to studyindependently and raises their comprehensive ability. The course assessment shows a good result.[
4.Nucleosome Induced Systemic Lupus Erythematosus-like Disease in BALB/c Mice
Jin HAO ; Fei HAO ; Baiyu ZHONG ; Shuqian TANG ; Feng PAN ; Jun GOU ; Yunsheng XU
Chinese Journal of Dermatology 1995;0(01):-
Objective To investigate the role of nucleosome in the pathogenesis of systemic lupus erythematosus (SLE). Methods BALB/c mice were immunized with nucleosome, and then serum dsDNA and ANA autoantibodies were detected by ELISA. Kidney specimens were observed by immunofluorescence and histological examination. Results High titers of IgG dsDNA and ANA autoantibodies in sera of BALB/c mice were observed at the 14th day after immunization with nucleosome. Nephritis and immune complex deposition in renal glomeruli were observed at the 35th day. Conclusion Nucleosome could induce SLE-like disease in BALB/c mice, and may play a critical role in the pathogenesis of SLE.
5.THE GROWTH PROPERTY OF NEURAL STEM CELLS ISOLATED FROM DIFFERENT PARTS OF MICE CNS——AN IN VITRO STUDY
Hanpeng XU ; Lin GOU ; Hao YANG ; Chunting WANG ; Yumei WU ; Gon JU
Acta Anatomica Sinica 1957;0(04):-
Objective To compare the growth property of the stem cells taken from different brain regions at the same developmental stage. Methods Mice embryos at the same development stage were isolated under sterile conditions, cortex, striatum, diencephalon, mid-hind brain and spinal cord were collected and pooled separately, after single-cell suspension obtained, different regions' cell suspensions were seeded in FGF supplemented serum-free culture medium. Followed the neural stem cell clone(neurospheres) fromation, immuno-cytochemistry method was utilized to identify the cell characteristics, all these clones were passaged under same conditions, clone formation and cell migration were observed under phase-contrast microscope. Results In the FGF added serum-free medium, neural cells experienced a large scale death within 48h after being seeded, then few single cells began to proliferate and formed the floating cell clones in the medium. These clones (neurospheres) could form new clones when seeded as single cell suspension. If these clones were seeded on poly-orithine, they could differentiate into neurons and glia cells. Compare the clone formation and cell migration, we found that: cortex, striatum, diencephalon all could form floating clones with different rate, the cortex formed clones at the highest rate, striatum and diencephalon at lower rate; few neurospheres formed from cortex adhered to the culture plate substrate and few cells were found migrating out from the adhered clones, striatum and diencephalon derived neurospheres adhered the plate more easily, and there were apparent cell migration. Mid-hind brain and spinal cord formed clones at the lowest rate, floating clones were scarce, and the clones adhered to the substrate readily, there were large amount of cell migrating out from these adhered clones. Conclusion Neural stem cells could proliferate and be passaged in vitro in serum-free medium, and they could be induced to differentiate under certain conditions into major cells types of CNS, there were differences in clone forming rate and cell migration between neural stem cells derived from different CNC regions, nonetheless they were at the same development stage, this may reflect that, in some degree, these cells can keep some of their region-specified developmental intrinsic property in vitro.
6.An immunohistochemical study of the effects of excessive fluoride on type I collagen in rat developmental dentine.
Yu-qing HAO ; Gou-quan WANG ; Zhong-ying NIU ; Xue-dong ZHOU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(6):429-431
OBJECTIVETo study the effects of excessive fluoride on type I collagen in rat developmental dentine.
METHODSEighty SD rats, 5 days old, were divided into experimental and control groups, 40 in each group. The experimental group received subcutaneous injection of 0.2% NaF every 4 days (the dose was 2 mg NaF per kg body wt). The same volume of 0.9% NaCl was used in the control. Twenty rats in each group were killed 4 days after the second and the seventh injection respectively. The expression of type I collagen was assayed with immunohistochemical technique.
RESULTS4 out of 20 rats after two injections showed abnormal distribution of type I collagen (dense stain of collagen in the odontoblast, aggregation of collagen in the dentine and disordered arrangement of collagen in the predentine; All 20 rats after seven injections showed abnormal distribution of type I collagen.
CONCLUSIONExcessive fluoride may affect the metabolism of type I collagen in rat developmental dentine.
Animals ; Animals, Newborn ; Collagen Type I ; analysis ; Dentin ; chemistry ; drug effects ; growth & development ; Fluorides ; toxicity ; Immunohistochemistry ; Rats ; Rats, Sprague-Dawley
7.Dose-effect relationship and time-effect relationship of T cell receptor gene mutation induced by γ-rays in human lymphocytes of peripheral blood
Xuesong QI ; Huimin Lü ; Chunyan WANG ; Wei ZHANG ; Shuxia HAO ; Qiao GOU ; Peng TONG ; Qingjie LIU ; Xu SU
Chinese Journal of Radiological Medicine and Protection 2011;31(3):286-289
Objective To study the dose-effect relationship and time-effect relationship of T cell receptor (TCR) gene mutation induced by γ-rays in lymphocytes of human peripheral blood.Methods Samples of peripheral blood were collected from 10 healthy adults and lymphocytes were separated.Four samples from males used to fit time-effect curve were exposed to γ-rays at the doses of 0,0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0,and 5.0 Gy,respectively,and 6 samples from 3 males and 3 females used to fit dose-effect curves were exposed to γ-rays of the dose of 2 Gy.Flow cytometry was used to detect the mutation frequency of TCR gene (TCR MF).Radiation dose-effect curves and time-effect curves were fitted and optimal mathematical models were selected respectively.Results The optimal mathematical model for radiation dose-effect was quadratic equation model:TCR MF = 92.14 + 22.61D2 (R2adj = 0.65).The optimal mathematical model for radiation time-effect was quadratic polynomial equation model:TCR MF = 3.74 + 743.66T + 308.64T2 (R2adj = 0.79).Conclusions TCR MF is increased as the γ-rayirradiation dose increases within the range of 0-5 Gy,and TCR MF is increased with the lapse of time within the range of 4 days after γ-ray radiation.
8.Expression of matrixmetalloproteinase-8 on the bell-stage in human and rat tooth development.
Yu-qing HAO ; Zhong-ying NIU ; Gou-quan WANG ; Xue-dong ZHOU ; Tao HU
West China Journal of Stomatology 2004;22(1):26-28
OBJECTIVETo study the expression of MMP-8 in human and rat tooth development.
METHODSImmunohistochemistry was used to detect the localization of MMP-8 protein while in situ hybridization was used to examine the expression of MMP-8 mRNA.
RESULTSThe expression of MMP-8 protein was localized in odontoblast and dentin matrix at the later bell stage in human tooth germ. The dentin was denser close to the pulp cavity. The expression of MMP-8 mRNA was found in very few polarized odontoblast at the early bell stage and all polarized odontoblast at the later bell stage in rat tooth germ.
CONCLUSIONThe results suggested that MMP-8 involved in dentin matrix rebuilding in the process of dentin formation in human and rat dental development.
Animals ; Animals, Newborn ; Dentin ; enzymology ; Embryo, Mammalian ; In Situ Hybridization ; Matrix Metalloproteinase 8 ; biosynthesis ; genetics ; Maxilla ; enzymology ; Odontogenesis ; RNA, Messenger ; biosynthesis ; genetics ; Rats ; Rats, Sprague-Dawley ; Tooth Germ ; embryology ; enzymology
10.Expression change and clinical significance of plasma miR-128-3p in patients with severe pancreatitis
Jiandong WEI ; Wei SHI ; Xiaojun GOU ; Hao JIANG
Chinese Journal of Endocrine Surgery 2022;16(5):611-615
Objective:To detect the expression and clinical significance of microRNA-128-3p (miR-128-3p) in the plasma of patients with severe pancreatitis (SAP) .Methods:A total of 175 patients with acute pancreatitis who were treated in our hospital from Jun. 2017 to Dec. 2020 were selected as observation objects. According to the severity of the patients, the patients were divided into 78 cases in mild acute pancreatitis (MAP) group, 50 cases in moderate to severe acute pancreatitis (MSAP) group and 47 cases in severe acute pancreatitis (SAP) group; according to the patient’s prognosis, the SAP group was divided into 28 cases in the survival group and 19 cases in the death group. The level of miR-128-3p was detected by qRT-PCR method, and patients were evaluated with Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) score and Ranson score, the correlation between plasma miR-128-3p level and APACHEⅡ, Ranson score was analyzed by Pearson, the predictive value of plasma miR-128-3p for poor prognosis of SAP patients was analyzed by ROC curve.Results:There were statistically significant differences in APACHE II[ (3.41±1.56) , (5.63±1.78) , (6.57±1.83) points], Ranson [ (2.58±1.34) , (4.95±1.47) , (6.06±1.92) points] and plasma CRP [ (39.73) ±12.31) , (70.25± 24.38) , (142.51±40.55) mg/L], serum calcium [ (1.95±0.31) , (13.26±5.24) , (14.21±6.32) mmol/L], among the MAP group, MSAP group and SAP group ( P<0.05) ; Compared with the MAP group (1.05±0.12) , the plasma miR-128-3p expression levels in the MSAP group (0.83±0.08) and the SAP group (0.57±0.05) were significantly decreased ( P<0.05) ; compared with the MSAP group (0.83±0.08) Compared with the SAP group (0.57±0.05) , the plasma miR-128-3p expression level was significantly decreased ( P<0.05) ; Compared with the survival group [ (0.65±0.08) , (5.91±1.64) points, (5.45±1.25) points, (120.43±30.56) mg/L], the plasma miR-128-3p of SAP patients in the death group was (0.43±0.05) expression levels were significantly reduced, APACHE II [ (7.43±2.21) points], Ranson score [ (7.22±1.59) points] and plasma CRP level [ (171.52±42.38) mg/L] were significantly increased ( P<0.05) ; the results of correlation analysis showed that plasma miR-128-3p level was negatively correlated with APACHEⅡ and Ranson scores ( r=-0.531, -0.609, P<0.05) ; The diagnostic efficacy of plasma miR-128-3p in predicting poor prognosis of SAP patients is better than APACHEⅡ, Ranson score, and CRP. Conclusion:Plasma miR-128-3p level is elevated in patients with severe pancreatitis, which is of certain value for the diagnosis and prognostic evaluation of SAP.