1.Effects of thyroid hormone on expression of 11?-hydroxysteroid dehydrogenase type 1 in hippocampus of newborn rats
Academic Journal of Second Military Medical University 2000;0(10):-
Objective:To observe the effect of thyroid hormones on 11?-hydroxysteroid dehydrogenase type 1(11?-HSD1) in newborn rats' hippocampus. Methods: Hypothyroid model was induced with propylthiouracil(PTU, 50 mg/d) in SD rats in the last week of pregnancy and was confirmed by measuring the levels of thyroid hormones and thyrotropin(TSH) in the newborn rats' blood. Western blot was used to determine the expression of 11?-HSD1 in hippocampus in newborn rats. Effects of triiodothyronine(T 3) and synthetic glucocorticoid dexamethasone(Dex) on expression of 11?-HSD1 in primarily- cultured hippocampus were studied with thin layer chromatography(TLC) and Western blot. Results: Thyroid hormone levels decreased and TSH level increased in the newborn rats' blood. The expression of 11?-HSD1 decreased by 41.6% in the hippocampus of newborn rats born to the mothers treated with PTU. T 3(10 -9 ,10 -8 ,10 -7 mol/L) and Dex(10 -7 mol/L) up-regulated the activity of 11?-HSD1 by 29.4%,45.6%,60.9% and 39.8%,respectively. T 3(10 -9 ,10 -8 ,10 -7 mol/L) and Dex(10 -7 mol/L) increased the expression of 11?-HSD1 protein by 26.0%,43.2%,64.9% and 41.1%,respectively.Synergistic effect of T 3 and DEX was observed in terms of regulating 11?-HSD1: the expression of 11?-HSD1 increased by 123.3% in the hippocampal neurons exposed to both Dex(10 -7 mol/L) and T 3 (10 -7 mol/L) and the activity increased by 114.1%. Conclusion:Thyroid hormone can up-regulate the expression of 11?-HSD1 in the hippocampus during brain maturation either by itself or synergistically with glucocorticoid, thereby enhancing the action of glucocorticoids on the brain.
2.Study on the Breeding of L-Arginine-producing Strain
Microbiology 1992;0(03):-
A L-arginine-producing mutant UN100-12(SG~(r),AE~(r)) was derived from Corynebacterium glutamicum ATCC138761 by combination treatment with ultraviolet(UV) and N-methyl-N-nitro-N-nitrosoguanidine(NTG). It could accumulate 16.6g/L L-arginine in the medium containing glucose as carbon source and ammonium sulfate as nitrogen source, when it was culrured at 30℃ for 4 days. And also this mutant has good stability of descendiblity of L-arginine.
3.Eeffect of transurethral bipolar and resection bicyclol Plasma cutting on treatment of high-risk BPH
Chinese Journal of Primary Medicine and Pharmacy 2009;16(8):1404-1405
Objective To explore the high-risk patients with benign prostatic hypeiplasia using bipolar transurethral resection of prostate for the treatment of dual-loop electrosurgical excision of the safety .effectiveness.Methods To retrospectively analyze of 56 cases with high-risk BPH patients with United States bicyclol CIRCON ion bipolar TURP TURP mirror line.Results Operation time was 50~100 minutes,an average of 70 minutes.1 case of mild postoperative incontinence,1PSS score from(28.2 ±1.5)to(7.0 ±1.0).voiding patency,fewer complications.Conclusion 1.Lavage using normal saline to avoid TURS syndrome,operative time can be extended accordingly.2.The current through the body,suitable for patients with special diseases.3.Reducing the incidence of capsularperforation.4.Less bleeding and reduce the occurrence of postoperative complications.5.TURP plasma removal of prostate tissue without charring,suitable for histopathological examination,to avoid the TURP and TUVP undetected pathological examination,increased prostate cancer detection rate.
4.Clinical characteristics and therapeutic strategy of severe craniocerebral injury in children
Chinese Journal of Applied Clinical Pediatrics 2014;29(6):413-415
The chance of craniocerebral injury in children is high.Severe craniocerebral injury is still the difficult point in current clinical treatment.Children severe head trauma is different from adults.It is characteristic of severe primary injury,rapid progressing secondary injury,confusing vital signs,less contrecoup injury,high incidence of epilepsy,cerebral ischemia in familiar,and good prognosis.This article focused on 2 hot issues in recent therapeutic strategy of severe craniocerebral injury in children——intracranial pressure monitoring and hypothermia therapy.The former is simple,convenient,safe and practical,is worth popularizing widely.
5.The study of estrogen receptor in bladder cancer and prostate cancer
Hao FU ; Zhi-Gang LUO ;
Cancer Research and Clinic 1997;0(03):-
Estrogen receptor(ER),an important transcription factor belonging to the nuclear receptor superfamily,comprises two subtypes ER?and ER?.Estrogen receptor is expressed in prostate cancer and ladder cancer and has a compacted relationship with them.In this review,we summarized the structure,distri- bution,function of different estrogen receptor subtypes and progress in study on relationship between different estrogen receptor subtypes and prostate or bladder cancer.
6.Clone, Expression and Characterization of the Thermostable ?-Galactosidase from Soil Metagenomic DNA of Taishan Mountain
Ping WEI ; Wen-Gang SONG ; Gang-Ping HAO ;
Microbiology 2008;0(11):-
A possible ?-Galactosidase gene(pwtsA) was discovered from soil metagenomic DNA of Taishan Mountain.PwtsA gene was inserted into the expression vector pET30a and transferred into E.coli BL21(DE3).Recombinant protein PWTSA was expressed as a soluble form at high level through IPTG induction,with a molecular mass of 57 kD analyzed by SDS-PAGE.PWTSA can produce o-nitrophenol from o-nitrophenol-?-D-galactopyranoside(ONPG),and its specific activity was determined as 13.6 U/mg.The enzymatic studies demonstrated that the recombinant protein PWTSA was a thermostable ?-Galactosidase,its optimum temperature and pH were 85?C~95?C and 6.5 respectively.In standard assays,the Km for ONPG was 0.83 mmol/L.
7.Research on the application of BioImp Vet Body composition in of the second class in the experiment teaching of medical function
Xiaomin DONG ; Xuehong TONG ; Gang HAO
China Medical Equipment 2013;(11):66-67,68
Objective:Through the introduction of BioImp Vet Body Composition principle and application of the second classroom experimental teaching practice in medical function, analyzer application characteristics of BioImp Vet Body Composition. Methods:We studied the distribution of body fluid and fat content determination of rats using BioImp Vet Body Composition. The device using the complex impedance measurement technique in vivo, within the second 4 k-1000 kHz scope, scanned the 256 frequency bands. We measured body water content (TBW), extracellular fluid (ECF) and intracellular fluid (ICF), in addition to the amount of fat (FFM) and fat mass (FM). Results:This measurement technique is very convenient, reliable and quick data acquisition in our second classroom teaching and laboratory. Conclusion:the measurement of BioImp Vet Body Composition can be used in the field of medical experimental teaching, has the application and characteristics of portable and feasible of the second class in the experimental teaching of Medical Function.
8.Relationship between paraoxonase 2 A148G polymorphism and diabetic nephropathy
Yunling HAO ; Lixiang UN ; Gang CHEN
Chinese Journal of Nephrology 1997;0(06):-
Objective To determine whether the paraoxonase2 (PON2) gene A148G polymorphism is associated with the pathogenesis of nephropathy in type 2 diabetes mellitus patients (DN) . Methods PON2 genotype distribution and allele frequency were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) . Serum immune reactive insulin (IRI) and C-peptide (C-P) concentrations were measured by radioimmunity. Results GG genotype and G allele frequency were higher in DN group than that in simple type 2 diabetes mellitus group (x2 =4. 26 P
9.Molecular mechanisms of brain development regulated by thyroid hormone
Rusong HAO ; Yue HOU ; Gang SUN
Chinese Journal of Endocrinology and Metabolism 1985;0(02):-
The pathologic changes of cerebral tissues in hypothyroidism and the molecular mechanism of brain development influenced by lower thyroid hormone levels are reviewed.
10.Relation between paraoxonase 1 gene Q191R polymorphism and type 2 diabetes mellitus complicated with hypertension
Yunling HAO ; Lixiang LIN ; Gang CHEN
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
Objective To investigate the relation between paraoxonase (PON) 1 Q191R gene polymorphism and type 2 diabetes mellitus complicated with hypertension. Methods Genomic DNA was extracted from peripheral blood leukocytes. The studied DNA was amplified by PCR, and then digested with the restriction endonuclease AlwⅠ and separated on 2% agarose gel electrophoresis. PON activity was estimated by spectrophotometer. Serum insulin and C-peptide were measured by radioimmunoassay. Results The frequencies of QQ genotype and Q allele in type 2 diabetes mellitus with hypertension group were significantly higher than those in type 2 diabetes mellitus group (P