Objective To explore the diagnosis,treatment and outcome of testicular torsion in children. Methods The clinical data (including the onset,treatment and prognosis) of 19 children of acute testicular torsion were reviewed.The age ranged from 5 days to 13 years. Results There were 15 cases of left side testicular torsion and 4 cases of right side.All of the 19 cases underwent operation.Seventeen cases were with intravaginal torsion,of these 14 testicles were removed for necrosis and only 3 testicles were salvaged by operative detorsion.Two cases were of extravaginal torsion with testicular necrosis and therefore were removed. Conclusions Testicular torsion is a common emergency of scrotum in children.Immediately operation after admission to hospital is the key treatment for salvage of the testicle.

Objective To observe the effects of carbachol(a cholinergic agent)on gastric emptying and gastric blood flow in resuscitation of early shock with oral administration of glucose-electrolyte solution(GES)in scalded rats.Methods Seventy-eight male Wistar rats were randomly assigned into five groups:shame scald(n=6),scald without fluid resuscitation(n=18),scald resuscitated with oral GES alone(n=18),scald resuscitated with oral carbachol alone(CAL,n=18),and scald resuscitated with oral carbachol plus GES(GES/CAL,n=18).About 35% TBSA scalded injury on back of rats was produced with boiling water(100℃,15s)in the latter four groups.GES(2ml/kg,which is equivalent to half volume of fluid infusion in first 24 hour according to Parkland' formula)was given twice in two-hour intervals by gavage beginning from 30min after the injury in GES and GES/CAL group.Carbachol(60?g/kg)was gavaged 30min after the injury in CAL and GES/CAL group.The gastric emptying rate was determined by phenol red emptying method and gastric blood flow was measured with laser Doppler at 2h,4h and 6h after the scald injury.Results The gastric emptying was obviously delayed and gastric blood flow was significantly reduced in scald animals compared with those in sham group(P

Objective To explore the apoptotic effect of Eqi compound prescription (contained-herb serum) on cute myelocytic leukemia HL-60 cell, which is relative to intracellular Ca2+, Caspase-3 and Bcl-2. Methods According to serum pharmacology, HL-60 cells were exposed to 10% concentrations of contained-herb serum for 24, 48, 72 and 96 hours respectively. Cells were observed under a fluore-scence microscope. SubG1 DNA was examined by flow cytometer. Intracellular Ca2+ concentration was measured by fura-2 fluorescence load method. Caspase-3 enzymatic activity were measured by colorimetry. Bcl-2 gene expression were measured by LSAB. Results The contained-herb serum could induce apoptosis of HL-60 cells. Intracellular Ca2+ concentration of treatment with Eqi was higher evidently than that of control (P

Objective:To produce monoclonal antibodies(McAb) against aspergillus fumigatus and to establish rapid assay for the measurement of aspergillus fumigatus antigen.Methods:Recombinant galactomannoprotein of aspergillus fumigatus(AFMP1) was used to immune BALB/c mice.Monoclonal antibodies against AFMP1 were produced from hybridoma.Results:Three hybridomas producing antibodies against AFMP1 were obtained.IgG isotypes of three McAb were IgG1,IgG2a and IgG2b.The affinity constants(K) were 1.2?10 10 ,4.56?10 9 and 1.81?10 10 mol/L.The antibodies were proved to be specific for aspergillus fumigatus by Western blot and recognized different epitopes on AFMP1 by the additivity assay.An sandwich enzyme-linked immunosorbent assay(ELISA) to detect AFMP1 was established to produce standard curve which showed linearity between 0.1～60.0 ng/ml with a sensitivity of 0.1 ng/ml.Conclusion:These results show three hybridomas producing high specificity and affinity monoclonal antibodies against AFMP1 and can provide for rapid assay for the measurement of aspergillus fumigatus antigen.

Objective To establish blood screening and genetic detection of thalassemia trait in pregnant couples in Chang‐shou area so as to provide guidance for aristogenesis and prenatal diagnosis .Methods A total of 1 760 pregnancy in maternal and child health hospital treated from January 2013 to October 2014 were selected for study .The component of hemoglobin was ana‐lyzed as primary screening and genotype of pregnant couples were ensured in which primary screening result is positive .Results There were 27 cases suspected as α‐thalassemia (positive rate was 1 .53% ) and 25 cases suspected as β‐thalassemia(positive rate was 1 .42% ) in the primary screening(n=1 760) .The positive rate of gene carrier were 31 .51% (n=438) in women and 33 .33%(n=27) in men .Conclusion The routine screening of thalassemia could guide aristogenesis in high incidence area and provide terms of prenatal diagnosis and genetic counseling .

Fifty-four children, aged 1.5-3.5 years, were selected from a nursery and divided into three groups. To the children in group 1 multivitamin fortified formular milk powder of Hai-He brand (70g daily) was given as a supplementary food in addition to the normal diet and whole milk powder of Hai-He brand was given to group 2 as another supplementary food. The third group served as control. This observation was lasted for three months. At the end of the third month, the increments of body weight of the children, tricep skinfold and circumference of upper arm of group 1 were higher than those of group 3 significantly, but there were no significant differences between group 1 and group 2. The hemoglobin content of group 1 increased by 0.8g/dl but group 2 and 3 decreased by 0.53g/dl and 0.72g/dl respectively. At the end of our observation, the concentrations of vitamin B1, C and PP in urine of group 1 were higher than those of other two groups by vitamin load test.Thus, we may consider that the multivitamin fortified milk powder is better than the whole milk powder in improving the vitamin and iron nutritional status of young children.

Objective To compare the therapeutic effect,advantages and disadvantages of 2 different methods on female stress urinary incontinence(SUI),vaginal wall suspension via percutaneous puncture(VWS-PP)and tension-free vaginal tape(TVT).Methods Fourteen SUI cases were treated by VWS-PP and 12 SUI cases were treated by TVT.The patients'age and course in 2 treatment groups were similar,and they were followed up 6 weeks,6 months and 1 year post-operatively.Therapeutic effect was evaluated by presence or absence of urinary incontinence when abdominal pressure increased.Meanwhile,operation time and complication were also compared.Results The therapeutic effect in VWS-PP group resembled as in TVT group,but the complication in VWS-PP was much less than that in TVT.The mean operation time of the VWS-PP group was 80 min(range from 60 to 90 min).while that of TVT group was 35 min(range from 25 to 50 min).The difference between 2 groups was statistically significant(P＜0.01).Conclusions The VWS-PP method is an effective,safe and mini-invasive operation for SUI.similar to TVT.Although the operation time in VWS-PP group is much longer than that in TVT group,the trauma is tinier and the surgeon can observe the anatomy reset by ultrasonic wave.

OBJECTIVE To study the etiological diagnosis of invasive aspergillosis by the monoclonal antibodies against Aspergillus fumigatus. METHODS An animal model of rabbit invasive aspergillosis was established.The antigen of A.fumigatus in serum was detected by ELISA.The antigen of A.fumigatus in tissue was detected by immunochemistry. RESULTS ELISA assay showed positive 24,48 and 72 hours after infection.Immunochemistry was positive 72 hours after infection. CONCLUSIONS The monoclonal antibodies against A.fumigatus has great potency usage.

Objective:To express SARS-CoV nucleocapsid protein in Bac-to-Bac Baculovirus Expression System and analyze the antigenicity of the recombinant protein.Methods: The SARS-CoV nucleocapsid gene was amplified by PCR.The PCR product digested with BamHⅠand SalⅠrestriction endonucleases was cloned into vector pFastBac HTC of Bac-to-Bac Baculovirus expression system.Recombinant plasmid was transformed DH 10Bac cells to obtain the recombinant Bacmid DNA.Recombinant Bacmid DNA was transferred into Sf9 cells which were inducted to express the recombinant protein in High Five cells.After purified by Ni affinity chroma-tography ,the antigenicity of the recombinant protein was analyzed by Western blot and ELISA.Results:Recombinant plasmid was con-structed successfully.The recombinant protein with the relative molecular mass of 48 kD was efficiently expressed in High Five cells and purified successfully by Ni affinity chromatography.Western blot and ELISA analysis showed that the recombinant protein could be spe -cifically recognized by the monoclonal antibody to SARS-CoV N protein and immune serum from rabbits ,respectively.The recombinant protein can specifically reacted with serum from SARS patients ,not with serum from healthy persons and patients infected with hCoV-229 E and hCoV-OC43.Conclusion: SARS-CoV nucleocapsid protein has been expressed successfully in the Bac-to-Bac Baculovirus Expression System ,and obtained good antigenicity.It is preliminary deemed that it can't reacted with serum from patients infected with hCoV-229E and hCoV-OC43.

Objective To compare clinical outcome of microvascular decompression (MVD) and percutaneous balloon compression (PBC) by using a prospective cohort study in order to provide a reliable evidence for the clinical decision-making. Methods Patients with trigeminal neuralgia hospitalized at Hangzhou First People′s Hospital in 2010 were chosen as database for cohort study. The patients were divided into MVD group (30 cases) and PBC group (30 cases). The clinical efficacy was followed by independent observers for 36 months after surgery. Chi-square test for hierarchical data, t test for quantitative data, and Kaplan-Meier plot for clinical outcomes were applied in the research. The endpoint was follow-up accomplishment or severe occurrence. Results Sixty patients were included in the research till the endpoint. The general records before surgery were almost the same with the literature records. By comparing painless period, mild and severe relapse, MVD group was superior to PBC group (P < 0.05). As for the painless survival period, MVD group was 96.7% of pain free after 1 year, 93.3% after 3 years, while PBC group was 90.0% after 1 year and 83.3% after 3 years. Regarding 3 years of follow-up, the relapse seemed occurred after 1 year in both groups. Conclusions As a curative and nondestructive procedure , MVD is more effective and has longer lasting pain free period , which should be considered as the first choice of treatment for trigeminal neuralgia in healthy people.